Monoclonal Antibody to Progesterone Receptor 11175 Flintkote Ave., Suite E, San Diego, CA 92121 Tel: (858) 642-0978 Fax (858) 642-0937 Toll free: 1-888-723-GENE E-mail: [email protected] Website: http://www.imgenex.com Monoclonal Antibody to Progesterone Receptor Catalog No : ABX-140A-0.1MG Contents : Progesterone receptor antibody at 0.2 mg/ml in PBS with 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. Isotype : Mouse IgG1, Kappa Clone : PR62 Protein G Chromatography was used to purify this Purification : progesterone receptor antibody Species React : Human Predicted React : Other species not tested Host : Mouse Application IHC (p): use antibody at 1:500 Storage Store antibody at 4°C, stable for 2 years. For long-term storage, aliquot and store at -20°C. Background The progesterone receptor (also known as NR3C3) is a member of the steroid or nuclear hormone receptor superfamily family of proteins. Family members are involved in diverse physiological functions including embryonic development, cell differentiation, and homeostasis. The receptors function by binding to DNA in the nucleus and regulating the transcription of target genes. The progesterone receptor is activated by its ligand progesterone, which is a steroid hormone involved in the menstrual cycle, pregnancy and embryogenesis. Progesterone receptor expression status is commonly used as a marker for classifying breast cancers and for predicting prognosis and response to endocrine therapy. That is, antibody positive versus antibody negative tumors can have potential implications with respect to predicted outcome, treatment, or disease monitoring. The antibody reacts with the progesterone receptor. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). These isoforms are generated from alternative transcriptional start sites within the progesterone receptor gene. Both forms are ligand activated by progesterone although they differ in their relative ability to activate target gene transcription. Antibody to progesterone receptor is widely used to study breast and other progesterone receptor containing tissues such as the endometrium. Researchers are encouraged to consult the scientific literature for additional information regarding the use of antibody to measure progesterone receptor as part of assessing breast or other tumor types. Antigen A progesterone receptor recombinant protein was used as the immunogen for the antibody. The specific epitope has not been mapped. Application Notes The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator. Formalin-fixed, paraffin-embedded normal human breast (left) and invasive ductal carcinoma (right) stained with progesterone receptor antibody (1:500), peroxidase-conjugate and DAB chromogen. 1 Monoclonal Antibody to Progesterone Receptor Reference 1. Cserni G, M Francz, E Kalman, G Kelemen, DC Komjathy, I Kovacs, J Kulka, L Sarkadi, N Udvarhelyi, L Vass, A Voros. Pathol Oncol Res 17:663-668 (2011). 2. Mote P, JF Johnston, T Manninen, P Tuohimaa, CL Clarke. J Clin Pathol 54:624-630 (2001). 3. Mylonas I, J Makovitzky, K Firese, U Jeschke. Acta Histochemica 111:349-359 (2009). Copyright © IMGENEX Corporation. All Rights Reserved Toll free: 1-888-723-4363 Fax: 1-858-642-0937 www.imgenex.com [email protected] Research purposes only. Not for diagnostic or in vivo use. 2