CLINICAL ENDOCRINOLOGY LABORATORY Sample Instructions & Pricing Instruction for samples: PLEASE SEND SERUM ONLY, no whole blood or red cells. Also, do not use Becton-Dickinson serum separator tubes; they will degrade the analyte, particularly progesterone, and invalidate results if sample is stored in these tubes. Overnight is OK if unable to transfer immediately. Volume Required: Testosterone: 2 mL serum each AMH, Estrone Sulfate, Progesterone or Inhibin: 1 mL serum each Cryptorchid Panel: 2mL serum Pregnancy Panel: 1 mL serum Granulosa Cell Tumor Panel: 3 mL serum. Shipping Instructions: Overnight on an icepack is highly recommended for inhibin assay or for any panel which includes inhibin. Overnight or second day air in winter without ice is OK to save on shipping costs. Progesterone, estrone sulfate, testosterone, and estradiol are all very stable and can be shipped regular mail (if not in a hurry for results). FedEx or UPS will deliver directly to the lab (building and room), while regular mail (US Postal Service) will go through the University Mail Division and may take up to one week until received in the lab, which may delay the results. Please complete the submission form and include with your shipment. SHIP TO: ENDOCRINOLOGY LABORATORY DEPT. of POPULATION HEALTH & REPRODUCTION 4206 VM3A 1 SHIELDS AVENUE DAVIS, CA 95616-5270 Frequency of Testing:  All tests are run on a weekly basis, except AMH, which will be run every other week for the time being.  Samples must be received in the lab by Wednesday of each week in order to receive the results that same week.  Results are reported on Thursday (progesterone and estrone sulfate only) and Friday (all others) if the sample was received by Wednesday.  Cost per assay includes interpretation and phone/fax charge. Credit card payment is required before the assays can be run. (Exceptions if approved include billing or credit card number held on file.)  Consultation with a veterinarian as required or requested. ***Please note that as of November 2012, we will no longer be offering individual estradiol testing as it has become cost prohibitive to run small sample sizes. If you have a large research project (>50 samples) and would like to request ad hoc pricing for the estradiol assay, please contact [email protected] for information. LABORATORY REFERENCE RANGES PROGESTERONE: Female: Absence of active luteal tissue: 0.1 to 0.5 ng/mL Borderline for the presence of luteal tissue: 0.5 to 1.0 ng/mL Presence of luteal tissue: > 1.0 ng/mL Adequate for the maintenance of early equine pregnancy: > 4.0ng/mL Indirect determination of pregnancy for camelids >2.0 ng/mL. ESTRONE SULFATE: Female: Standard size mares: non pregnant or pregnant (first 45 days of pregnancy) 0.1-6.0 ng/mL Pregnancy of 45 days or greater > 6.0 ng/mL Miniature mares: non pregnant or pregnant (first 80 days of pregnancy) 0.1-60.0 ng/mL Pregnancy of 80 days or greater > 60.0 ng/mL Males: Cryptorchidism (not recommended, suggest use testosterone serum levels) Estrone Sulfate laboratory reference ranges for equine: Gelding <0.1 ng/mL; Cryptorchid 35-60 ng/mL; Stallion 140 – 200 ng/mL TESTOSTERONE: Male: Cryptorchidism determination From single serum sample: Equine laboratory reference ranges: Gelding < 50 pg/mL; Cryptorchid 100-500 pg/mL; Mature stallion 800-2000 pg/mL. hCG stimulation test Protocol: Equine: Baseline blood sample followed by the administration of 2500 IU of hCG IM or IV. 2 hours post-stimulation draw a second blood sample. Small animals: Baseline blood sample followed by the administration of 50 IU/Kg of hCG IM or IV. 2 hours post-stimulation draw a second blood sample. INHIBIN: EQUINE ONLY Mare: Normal ranges for a non-pregnant mare 0.1 – 0.7 ng/mL. Mares with inhibin levels > 0.7 ng/mL consistent with granulosa cell tumor or late term pregnancy (usually greater than 7 months). Stallion: Normal range 2.2 – 3.4 ng/mL AMH: EQUINE ONLY Mare: Normal ranges for a non-pregnant mare 0.1 – 3.8 ng/mL Borderline for a granulosa cell tumor 3.8 – 4.2 ng/mL Levels consistent with granulosa cell tumor > 4.2 ng/mL Cryptorchid levels > 0.15 ng/mL SUBMITTAL FORM AND PRICE LIST (effective 11/1/12) CLINICAL ENDOCRINOLOGY LABORATORY VM: Population Health & Reproduction 4206 VM3A, 1 Shields Avenue, Davis, CA 95616-5270 Phone: 530-752-0298 FAX: 530-752-6318 www.vetmed.ucdavis.edu/phr/endolab □ Pregnancy Panel ($40.00) □ Cryptorchid Panel □ GCT Panel ($90.00) ($75.00) (Prog / Estrogen Sulfate) (Testosterone / AMH) (Inhibin / Testo / Prog) □ Progesterone ($22.00) □ Testosterone ($29.00) □ AMH / Inhibin / Testo ($130.00) □ Estrogen Sulfate ($25.00) □ Inhibin ($57.00) □ AMH / Inhibin ($100.00) □ AMH ($60.00) Client Information (PLEASE print clearly) Customer No: 3-VPHR__________________________ Clinic/Hospital Name: __________________________________________________________ Address: _____________________________________________________________________ City: ______________________________ State: ________ Zip Code: ______________ Contact: Dr. ______________________________ Phone: ____________________________ Patient Information: Owner: ___________________________ Animal name: _____________________________ Animal/Reference #:__________________________ Species: _________ Age: _______ Sex: □ Male □ Female □ Intact □ Castrated □ Spayed □ Mini-equine Date collected: ____________ Last breeding date: _____________ History: ___________________________________________________________________________________________________________ _____________________________________________________________________________________________________________________ _____________________________________________________________________________________________________________________ Receive Results: □ Fax: ___________________________ □ Email: _____________________________________ Payment Information Signature: ___________________________Date:_____________ □ MasterCard □ Visa Credit Card # - - - Expiration Date: __________