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MICROBIOLOGY 20
LABORATORY SCHEDULE
SPRING 2015 - SECTION #5141 WEDNESDAY LAB)
BERN PREGERSON
LECTURE TEXT: Alcamo’s Fundamentals of Microbiology. Pommerville, 9th Ed., 2011
LABORATORY MANUAL: Benson, Harold. Microbiological Applications: Customized Version.
McGraw-Hill.
WEEK OF:
TUESDAY
WED.
FEB. 11
FEB. 18
LAB MANUAL
Exercise numbers in
lab manual
EXERCISES
S1=session 1, S2=session 2, etc.
Handout
Handout & Ex. 1
Laboratory protocol
Microscopy
Hay infusion (Demonstration)
Handout
Neisseria gonorrheae
Ex. 6
The Protozoa
Handout
A Parasitic protozoan, Toxoplasma gondii
LECTURE AND LAB QUIZ #1
Handout, (My Web
Pages & Pommerville
Textbook,p.66-69)
Biofilms
6
Bacteria-S1 (Session 1)
Handout
Bacillus anthracis (Lecture and Demonstration Slides)
FEB. 25
LECTURE AND LAB QUIZ #2
MARCH 4
6
10,11
Handout
Bacteria–S2
Smear Preparation and Simple Stain:
Corynebacterium diphtheriae
NOTE: LECTURE AND LAB EXAM #1, TUES. MARCH 10
MARCH 11
9
10,14
Pure culture technique-S1
Gram stain
MARCH 18
9
Handout
16
LECTURE AND LAB QUIZ #3
Pure culture technique-S2
Lytic action of tears, Lysozyme S1
Acid fast stain: Mycobacterium tuberculosis and Mycobacterium leprae
LECTURE AND LAB QUIZ #4
MARCH 25
Handout
Lytic action of tears, S2
36,37
37
17
10, 14
Bacterial unknowns-S1
a. Temperature
b. Nutrient Gelatin deep
c. Motility
d. Gram Stain
Handout
Clostridium botulinum (Demonstration Slide)
Demonstration slide of motility
LECTURE AND LAB QUIZ #5
36, 37
17
24
Bacterial unknowns-S2
a. Temperature
b. Nutrient Gelatin deep
c. Motility
With unknown: S1
a. Effects of oxygen on growth (the use of the anaerobe jar)
With unknown:S1 (Are these enzymes present)?
APRIL 1
38
38
38
38
b. Oxidase test
c. Catalase test
With unknown: S1 (Can your unknown ferment these sugars)?
d. PR Glucose
e. PR Lactose
With unknown: S1 The results will be determined when you return.(2
possible waste products if cells ferment glucose)
f. MR (methyl red test) – S1
g. VP (Voges-Proskauer) – S1
38
38
APRIL 4-10
SPRING BREAK
38
38
38
APRIL 15
39
39
39
With unknown:(2 possible waste products if cells ferment glucose)
a. MR (methyl red) – S2
b. VP (Voges - Proskauer) – S2
With unknown:
c. Nitrate test S1
With unknown: (Presence of hydrolytic enzymes)
d. Starch plate - S1
e. Urea broth - S1
f. Tryptone broth - S1
With Unknown: (Miscellaneous tests)
g. Kliger's iron agar deep - S1
h. Simmon's Citrate slant - S1
40
Alcohol + Disinfectants evaluation - S1
32
Bacterial population counts:
The plate count-S1
20
APRIL 22
38
39
39
40
40
40
NOTE: LECTURE AND LAB EXAM #3, TUES., APRIL 21
With unknowns:
a. Nitrate Test - S2
b. Urea broth - S2
c. Tryptone broth - S2
d. Kliger's iron agar deep - S2
e. Simmon's Citrate slant - S2
f. Starch plate
32
Alcohol + Disinfectants evaluation – S2
33
Antimicrobic sensitivity testing:
Kirby Bauer Method-S1
20
Bacterial population counts:
The plate count-S2
LECTURE AND LAB QUIZ #6
33
Antimicrobic sensitivity testing:
Kirby Bauer Method-S2
47, Handout
Water bacteriology – S1
Handout
Genetic engineering: Bacterial Transformation-S1
APRIL 29
LECTURE AND LAB QUIZ #7
MAY 6
MAY 13
47, Handout
Water bacteriology – S2
Handout
Genetic engineering: Bacterial Transformation-S2
Handout
Pyogenic gram positive cocci:
The staphylococci and the Streptococci-S1
47, Handout
Water bacteriology – S3
Handout
Pyogenic gram positive cocci:
The staphylococci and the Streptococci-S2 (Lecture and do Coagulase
Test for Staph.S1)
Handout
Handout
58
MAY 20
MAY 27
LECTURE AND LAB OPTIONAL QUIZ
The staphylococci – The coagulase Test S2
Nosocomial infections
White Blood Cell Study
59
ABO blood grouping
QUIZ SCHEDULE
QUIZ #1 WED. FEB. 25
QUIZ #2 WED. MARCH 4
QUIZ #3 WED. MARCH 18
QUIZ #4 WED. MARCH 25
QUIZ #5 WED. APRIL 1
QUIZ #6 WED. APRIL 29
QUIZ #7 WED. MAY 6
OPTIONAL QUIZ WED. MAY 20
EXAMS
EXAM #1 TUES. MARCH 10
EXAM #2 TUES. APRIL 21
EXAM #3 TUES. MAY 12
FINAL EXAM - INCLUDES BOTH LECTURE AND LAB MATERIAL
TUESDAY JUNE 2, 5-7 P.M.