The HIG Dual Injector
Upgrade Kit
®
Installation & Owner’s Manual
Overview
This user’s guide will describe the procedure for assembling, installing, aligning,
and using the HIG Dual Injector Upgrade Kit. Please keep in mind that not all GC’s are
identical, and there may possibly be some adjustments that need be made to your
individual instrument before this kit can be installed or used. In most cases, these
adjustments are minimal and can be done in a matter of minutes. If however, you happen
upon a problem that is a little more serious in nature, or if you need some sort of
technical assistance, you can call Horizon Instrument Group at (225)296-4966. Our hours
of operation are Monday – Friday, 8:00am to 5:00pm Central time. If there is an
emergency after hours or while our office is closed then please leave voice mail and one
of our on-call technicians will be in touch shortly afterwards.
NOTE: This kit is designed for use with the Agilent® 5890 series GC, Shimadzu® AOC20i Auto Injector Tower, and the AOC-20s Auto Sampler. It is not intended for use with
any other types of instruments. Horizon Instrument Group assumes no responsibility
whatsoever for any damages that might occur as a result of misuse of this product.
Shimadzu® is a registered trademark of Shimadzu Corporation.
AOC-20® is a registered trademark of Shimadzu Corporation.
Agilent® is a registered trademark of Agilent Technologies, Inc.
Horizon Instrument Group® is a registered trademark of Horizon Instrument Group.
HI-1200® is a registered trademark of Horizon Instrument Group.
Diplomat® is a registered trademark of Horizon Instrument Group.
©2002 Horizon Instrument Group – Patent Pending
Parts List
Qty.
1
1
1
1
2
2
2
2
2
2
1*
1*
1*
Reference #
A
B
C
D
E
F
 1
 2
 3
 4
G
H
I
See Appendix I
* = Optional Item
Part Number
HI1201-1
HI1201-2
HI1201-3
HI1002-1
HI1003-1
See Appendix II
HI1000-1
HI1000-2
HI1000-3
HI1000-4
HI1000-AL
DIPLOMAT
HI1201-T
HI1000-S
Description
Main Plate Assembly
Front Mounting Plate Assy.
Rear Mounting Plate Assy.
Start Out/Ready In Cable
Standoff
Mounting Pin #1
Mounting Pin #2
Mounting Pin #3
Mounting Pin #4
Alignment Tool
The Diplomat Translator
AOC20s Tray Bracket
Single Mounting Plate
Installation
NOTE: Before beginning installation, please check to make sure that the kit arrived with
all of the components. If there are any damaged or missing components, please contact
Horizon Instrument Group.
Step 1 – Mounting the Base Plate
To mount the plate to the instrument, you will need a Phillips screwdriver, 4
mounting screws, the Base Plate Assembly, and two standoffs (Also provided.).
Remove the existing injector plate if there is one, in a manor consistent with the
manufacturer’s instructions. If there are standoffs being used to support the original plate,
they may be used to support the HI1200 Base Plate Assembly as well. If there were no
standoffs or no previous plate, then screw in the standoffs (HI1003-1) provided in the kit
in the appropriate holes on the top of the instrument as illustrated below.
Stand-Off Positioning
Set the Base Plate Assembly in the proper position so that the injection ports sit
directly in the center of the large holes in the plate. The mounting screw holes in the plate
should directly line up with the holes in the instrument and in the standoffs. Use the
provided mounting screws to fasten the base plate to the instrument. Do not put screws
into the stand-offs yet. They will be added later in the installation. (NOTE: If there is any
split inlet tubing routed to the injection port(s), it should be removed until later in the
installation.)
Step 2 – Preparing And Installing The Mounting
Plate Assemblies
You are now ready to install the two mounting plate assemblies. For this
procedure you will need a Phillips screwdriver, 8 fastening/mounting screws, 2 alignment
tools (HI1000-AL), both front and rear mounting plate assemblies, and mounting pins.
First, the sliding plates must be prepared for installation. Secure the mounting
pins in the correct places on each of the plates as shown in the picture below.
Correct Pin Layout
(see Appendix I for alternative model)
NOTE: Pins 2 and 3 on both plates are very similar. They can be distinguished by closer
examination and noting that pin #2 has a slightly larger diameter than pin #3 has.
Once the pins are correctly positioned, the plates can be mounted to the base plate
and properly aligned. Due to the placement of the injectors and limited walk-around
space with most GC’s, it is suggested that the rear plate is installed first. Start by setting
the rear mounting plate assembly on top of the base plate at the rear position. Once the
rear plate is in place the alignment tool can then be used. Place the alignment tool so that
the septa nut fits closely inside the inner diameter of the tool. The alignment tool should
also seat in the mounting plate and at this point will not move. Make sure the opening in
the bottom of the alignment tool is aligned with the channel in the mounting plate so that
gas lines may be routed through if needed. The illustration on the next page shows what
the plates should look like once they are in place with the alignment tools.
Correct Plate Installation
Step 3 – Mounting The Injectors
The auto injectors are now ready to be installed. For this procedure, you will not
need any tools. However, it is recommended that you install the injectors from rear to
front. This will simplify the procedure a great deal.
Mount the rear injector on the rear mounting plate with the injector facing the rear
of the GC. The injector should sit all the way down on the alignment tool and pins. The
top of the alignment tool should fit tightly into the bottom of the injector. Once this is
achieved, no further means of alignment or installation is necessary. Keep in mind this
will be a tight fit due to the precision dimensions of the alignment tool, mounting pins,
and the injector. Refer to the following illustration to get an idea of what it should look
like and how these components should mate up to each other.
To mount the front injector, repeat the previous steps. The only difference is that
you will need to have the front injector facing the direction as the GC. As above, once the
injector is correctly seated, no further alignment is necessary. You are now ready to begin
connecting the cables to the system.
Step 4 – Connecting The Cables
NOTE: BEFORE CONNECTING ANY CABLES TO THE SYSTEM, MAKE SURE
THAT THE POWER IS TURNED OFF.
First you will need to find a suitable place to put the injector controller. Once this
is done, you will need to connect the controller cables from the front and rear ports of the
controller to the front and rear injectors, respectively.
NOTE: Make sure that the front and rear controller cables are connected to the correct
corresponding injectors. Failure to do so may result in improper operation of the auto
sampling system.
Next, connect the Ready In/Start Out cable between the controller and the
corresponding plug on the GC which is located on the top-right hand side. You may need
to remove the panel to access this area.
You are now ready to begin installation and configuration of the Diplomat®
Translator Device. For this installation, you will need to refer Step 5 – Installing and
Configuring The Diplomat® Translator Device.
Step 5 - Installing and Configuring The
Diplomat® Translation Device
The Diplomat® Translator Device allows your chromatography software package
to communicate via RS-232 to the Shimadzu® controller module. This is accomplished
by accepting the Agilent® control commands and translating them to the appropriate
Shimadzu commands. This means that if you are using a LIM system, you can use any
chromatography software that is compatible with the Agilent 7673® Auto Sampling
System thru RS-232 communications protocols. If your system uses GPIB or HPIB as a
protocol, the Diplomat can be configured to function with these as well by switching
DIP-switch #5 to the ON position (See next page).
One configuration setting that needs to be made by the user is for baud rate. This
is the speed at which the PC communicates with the Diplomat® Translator Device. This
is set up through a series of DIP-switches that are located on end of the device. The baud
rate on the Diplomat should be set to match that of the work station it is connected to.
The following table illustrates the different combination of switch positions and their
corresponding baud rates.
NOTE: If you’re not sure what the baud rate is on your workstation, consult the
appropriate technical personnel or the manufacturer’s software manual to learn how to
view this setting.
HPIB
1
2
3
4
Address
Position
5 on DIP-switch
= OFF (RS-232
Mode)
Baud Rate Settings (0 = OFF, 1 = ON)
Switch Position
1
2
3
Baud Rate
0
0
0
300
1
0
0
600
0
1
0
1200
1
1
0
2400
0
0
1
4800
1
0
1
9600
0
1
1
19200
1
1
1
28800
Switch 4: 1 = No Parity, 8 Data Bits, 1 Stop Bit
0 = Even Parity, 7 Data Bits, 1 Stop Bit
HPIB Mode
If your system utilizes the HPIB/GPIB protocol as a means of communication,
then the following will apply to you. To set the Diplomat for HPIB mode set position 5
on the DIP-switch to ON. This will allow the Diplomat to receive commands from an
HPIB to RS-232 converter. When switch 5 is set to ON, then the other positions
determine the HPIB address of your instrument, rather than the baud rate and parity
settings. The following table describes how to adjust the address setting for HPIB mode.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
0
1
0
1
0
1
0
1
0
1
0
1
0
1
0
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
Other Configuration Notes
In many cases, it may be necessary to make some changes to the configuration of
the Shimadzu injector tower. Below is a small table listing some of these functions and
what they do. You should refer to the Shimadzu AOC20i Instruction Manual for
directions on how to change these settings.
Function
F80
Set To
004
Description
Baud Rate (1=1200, 2=2400, 3= 4800, 4=9600)
F81
F82
F83
F84
F91
0
0
0
0
1
Parity (0=No Parity, 1=Even Parity)
Stop Bit (0=1 Stop Bit, 1=2 Stop Bits)
Protocol (0= Simple Procedure, 1=Level 2)
0=Transmission By Simple Procedure, 1=AOC-17 Compatable
Ready Signal Polarity (0=Open, 1= Closed)
A Note On Injection Volume
Due to differences in the way that an Agilent 7673 system and the Diplomat handle
certain information, you must make sure that the nano-liter adapter option in your
software configuration settings is enabled. Failure to do so will result in the injection
volumes being inaccurate. As long as the nano-liter adapter option is enabled, then the
volume specified in the software package will be the volume injected.
A Note About The Sample Racks…
In the event that you are using an AOC-20s Auto sampler tray, there are a few
things you will need to keep in mind. In order for the AOC-20s to work properly, the
sample racks for the injectors had to be slightly modified. This is so that the AOC-20s
will reach the sample vial position. The two figures below show the proper places for the
sample module (1) and the Wash/Rinse vial modules (2), depending on whether you are
using them on the front or rear.
Note: If you are using one injector for both ports on the GC by moving it from front to
back (requires plate #HI1000-S), then the front rack configuration will be used for
regardless of whether the injector is on the front or rear position.
Installing the Auto Sampler Tray (Optional)
In applications that use more than six samples, it becomes necessary to use an
auto sampler to handle the vials. If this is the case, Horizon Instrument Group has
developed a bracket that will allow for use of the AOC-20s Auto sampler. Below is a
summary of how to install this piece of equipment.


Mount the bracket onto the bottom of the Shimadzu AOC-20s Sampler.
Remove the screws on the left side of the Main Plate Assembly.






Mount the bracket assembly to the GC so that the screw holes correspond to the
holes in the bracket.
Replace the mounting screws so that they stabilize both the main plate assembly
and the auto sampler tray bracket.
Make sure the auto sampler tray is resting at a level position by adjusting the
cushioned levelers on the underside of the bracket.
Plug the auto sampler cable into the back of the Shimadzu controller.
Refer to the Shimadzu AOC-20s manual for further preparations.
Refer to the page titled Training The AOC-20s Of Vial Locations for
instructions on how to train the auto sampler of where to pick up and drop the
vials..
Training The AOC-20s Of Vial Locations
1) Turn off power to AOC controller module.
2) Depress and hold both the [MONITOR] and [FUNCTION] buttons on the main
AOC-20i injector for 10-15 seconds as the controller is powered up. *Before
doing anything else, allow the AOC to complete the self-test which usually lasts
50-55 seconds.
3) Set FUNCTION 78 on the main AOC-20i unit to “000”, then press enter.
4) Place sample vial (assembled with septum and cap) in the left-most position of the
AOC-20i rack.
5) Center the AOC-20s gripper over the vial, without raising or lowering the
gripper’s arm assembly.
6) On the main AOC-20i, use the [SAMPLE WASH] button to lift, or the
[NUMBER OF INJECTIONS] button to lower the gripper’s arm assembly. Use
the [SOLVENT WASH] button to stop the raising or lowering motion. Position
the lower tips of the gripper approximately 2-4mm below the top of the vial cap.
7) Set FUNCTION 78 on the main AOC-20i unit to “001”, then press [ENTER].
*Before continuing allow the AOC to complete the programming process which
lasts ~5 seconds.
SUB-Injector
8) Set FUNCTION 78 on the main AOC-20i unit to “002” then press [ENTER].
(Allow the sub AOC-20i to position it’s vial rack toward the AOC-20s.)
9) Remove the sample vial from the main AOC-20i rack and place it in the left-most
position of the sub AOC-20i vial rack.
10) Center the gripper over the vial, once again making sure not to raise or lower the
gripper’s arm assembly.
11) On the main AOC-20i, use the [SAMPLE WASH] button to raise or the
[NUMBER OF INJECTIONS] button to lower the gripper’s arm assembly. Use
the [SOLVENT WASH] button to stop the raising or lowering motion. Position
the tips of the gripper to approximately 2-4mm below the vial cap.
12) Set FUNCTION 78 on the main AOC-20i unit to “003” then press [ENTER].
(Once again, allow the AOC to complete the programming process which lasts ~5
seconds.)
13) Recycle the power on the entire AOC/GC/Data processing system.
Note: If you are using only one injector and switching it between the front and rear ports
on the GC, then use steps 1-7 of each position. If you are using a dual injector
configuration use steps 1-7 for the front and steps 8-13 for the rear.
Appendix I
For those customers that wish to use only one tower so that they may move it
from the front to the rear injection port, we have designed a plate that will allow such an
application. If you have this plate, then both of the autosamplers will be facing the front
of the GC rather than opposite ways. Below is an illustration showing the pin
configuration for this model.
****NOTE: You will notice that for the rear configuration, there isn’t a threaded hole
for pin 2A. We have included a special pin with no threads for this position. Simply fasten
the other pins in their proper positions, then, as you’re setting the autosampler on the
pins, place pin 2A in the proper spot, and let the autosampler rest on it. If you should
happen to misplace or loose pin 2A or any of the others, call Horizon Instrument Group
to order replacements.
Appendix II
Below is an illustration of the mounting pins so that you may be able to distinguish pins
1-4 from each other.
WARRANTY INFORMATION:
Installation & Use: Before beginning installation, please verify that the HIG1300 Sampler Controller arrived with all of the components. If there are any damaged or
missing components, please contact the Horizon Instrument Group ®.
Limit of Liability: The HIG Diplomat™ is designed for use with the Shimadzu
AOC 20i/s ALS and the 5890 GC. Horizon Instrument Group ® assumes no responsibility
or liability for any damages that may occur as a result of normal use, misuse, or abuse of
the HIG-1300 (HIG Diplomat™) Sampler Controller.
Warranty: The HIG-1300 (HIG Diplomat™) includes a One Year Warranty
sampler controller exchange. We do not warrant your autosampler or gas chromatograph.
Support: Please keep in mind that not all GC’s are identical, and it is possible
some adjustments are needed for your individual instrument before the HIG-1300 can be
installed and used. In most cases, these adjustments are minimal and can be done in a
matter of minutes; however, if you happen upon a problem that is more complicated and
need technical assistance, please contact the Horizon Instrument Group ® for support.
Service: Horizon Instrument Group® offers an exchange program for the HIG1300 during the warranty period. Post one year “Extended Warranty” Exchange
Agreements may be purchased after the original product warranty expires.