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Proteomics course: gel based protein separation by twodimensional
gel
electrophoresis
and
protein
characterization by MALDI-TOF/TOF mass spectrometry
15 – 18 June 2015
CIIMAR – Interdisciplinary Centre of Marine and Environmental Research, Univ. Porto
Ipatimup - Institute of Molecular Pathology and Immunology of the University of Porto
i3S - Instituto de Investigação e Inovação em Saúde
FCUP - Faculty of Sciences, University of Porto
ESTSP-IPP – School of Allied Health Technologies, Polytechnic Institute of Porto
Introduction: Proteomics is a research area devoted to the analysis of proteins from
complex biological samples. It has been applied to evaluate protein expression, to identify
proteins and characterize their post-translational modifications (PTMs). Examples of such
applications include the analysis of biochemical changes in organisms when exposed to an
adverse environmental condition, allowing a better understanding on organism’s response
and adaptation. Another major application of Proteomics is the discovery of novel diseaseassociated biomarkers with relevant clinical purposes. Two main approaches to undertake
proteomic studies are protein separation by two-dimensional gel electrophoresis (2DE)
and protein identification and characterization by MALDI-TOF/TOF mass spectrometry.
Course aims: This course aims to describe the principles of 2DE and MALDI-TOF/TOF mass
spectrometry as well as to provide basic training on protein separation by 2DE, peptide
analysis, protein identification and characterization of post-translational modifications by
mass spectrometry. English will be the speaking language.
Participants: graduated students and researchers that have interests in the field of
proteomics, 2DE and MALDI-TOF/TOF mass spectrometry analyses. Participants are
limited to 20 but a minimum of 10 participants will be required to start the course.
Equivalency to 3 ECTS can be solicited by the students following post-graduate courses at
Porto
University
(for
more
information
contact
Alexandre
Campos,
[email protected]).
Coordination:
The course will be held in CIIMAR and Ipatimup/i3S research institutes and coordinated by
Alexandre Campos (CIIMAR), Hugo Osório (Ipatimup/i3S), Marisa Freitas (Department of
Environmental Health, School of Allied Health Technologies, Polytechnic Institute of Porto)
and Vitor Vasconcelos (Faculty of Sciences, University of Porto and CIIMAR).
Registration: the registration fee is 200 euros. To register please contact Marisa Freitas:
email: [email protected]
Tel: +351 223401813
Preliminary Programme:
Monday, June 15th:
9.00 am – 13.00 pm: lectures (CIIMAR)
9.00 Welcome reception
9.30 Introduction to proteomics
10.10 Principles of protein sample preparation and 2DE
10.50 Coffee break
11.10 Principles of MALDI-TOF/TOF MS and protein identification
11.50 Protein quantification in proteomics
12.30 General discussion
13.00 Lunch
14.30 pm – 18.00 pm: laboratory work (CIIMAR)
Sample preparation and protein separation by Isoelectric Focusing (IEF) (First dimension)
Tuesday, June 16th:
8.30 am – 13.00 pm: laboratory work (Ipatimup / i3S)
- Protein isolation and “in gel” digestion
- Sample application in the MALDI plate
- Mass spectra acquisition in MS and MS/MS modes
- Mass spectra interpretation and protein identification
14.30 pm – 18.00 pm: lectures (CIIMAR)
Presentations provided by guest researchers, focusing the research in proteomics and the
different subjects covered by this discipline.
Wednesday, June 17th:
8.30 am – 13.00 pm: laboratory work (CIIMAR/Ipatimup/i3S)
- Second dimension SDS-PAGE
- Protein identification by homology search
14.30 pm – 18.00 pm: lectures (CIIMAR)
Second course of presentations provided by guest researchers, focusing the research in
proteomics and the different subjects covered.
Thursday, June 18th:
8.30 am – 13.00 pm: Lectures (CIIMAR)

2DE gel analysis using the software PDquest (Biorad)

Free software tools for the analysis of MS data
Sponsors: