Thermo Scientific Lunchtime Workshop Monday Date/Time: Monday, March 23, 12:45–13:45hrs Room: Hall 2 Of Proteins, Peptides and Others Big and Small with Orbitraps Big and Small This workshop will describe recent innovation in the analysis of both intact and digested proteins and other molecules on different Orbitrap mass spectrometer platforms. Shannon Eliuk will describe the sophisticated workflows possible on the Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer enabling discovery in the fields of proteomics, glycomics and glycoproteomics. Prof Albert Heck will discuss his experience in the field of analysis of intact proteins and proteins complexes as well as his recent authoritative work in the area of the proteomics of cellular reprogramming. From Proteins to Omes: Supreme Versatility for Qualitative and Quantitative Analysis Dr. Shannon Eliuk, Thermo Fisher Scientific Proteomics of Cellular Reprogramming Prof. Dr. Albert J. R. Heck, Biomolecular Mass Spectrometry and Proteomics; Utrecht, The Netherlands Exploring the Benefits of Unrestrained Mass Range and High Resolving Power on an Orbitrap (Jumbo) for Top-Down and Native Analysis of Proteins and Protein Complexes Prof. Dr. Albert J. R. Heck, Biomolecular Mass Spectrometry and Proteomics; Utrecht, The Netherlands Thermo Scientific Lunchtime Workshop Tuesday Date/Time: Tuesday, March 24, 12:45–13:45hrs Room: Hall 2 High Throughput, Optimized Shotgun Proteomics with DDA and DIA on the Thermo Scientific™ Q Exactive™ For most of the short history of proteomics mass spectrometry-based workflows have operated via data dependent LCMS experiments. However, in the last several years there has been growing interest in workflows absent of data dependent mass spectral acquisition. These approaches have come to be known as data independent analysis (DIA). In addition, a great deal of development has occurred up-front of the mass spectrometer to optimize and simplify proteomics workflows. In this workshop two speakers, Prof Matthias Mann and Dr Yue Xuan, will discuss recent developments in optimizing shotgun proteomics experiments. Prof Mann will focus on achievements up-front of the mass spectrometer aimed at maximizing up-time and throughput while Dr Xuan will focus on the data acquisition approach. DIA Aalysis on the OrbitrapPlatform - Using Novel Data Independent Analysis Methods to Obtain Characterization of a Biological Pathway Yue Xuan, Thermo Fisher Scientific Technology Development towards High-Throughput Proteomics Prof. Dr. Matthias Mann, MPI of Biochemistry, Munich, Germany