Prokaryotes 1. Bacteria are classified into two kingdoms: Eubacteria (true bacteria) and Archaebacteria (Ancient Bacteria). 2. They lack of a cell nucleus and other organelles with double membrane 3. Region called the NUCLEOID which has a single circular chromosome, accessory rings of DNA called PLASMIDS binary transverse fission and budding Each bacterium divides following unequal cell growth; the mother cell is retained, and a new daughter cell is formed. Most bacteria reproduce by a process of binary transverse fission Sporulation – endospores in Bacillus and Clostridium – cysts in Azotobacter – heterocysts in some cyanobacteria. Bacterial gene exchange pilius CONJUGATION (exchange of DNA) TRANSFORMATION- bacteria incorporate genes from dead bacteria TRANSDUCTION - viruses insert new genes into bacterial cells Inclusions Inclusions are reserve deposits found in prokaryotic and eukaryotic cells. • • • • • inorganic phosphate polysaccharide granules lipids reserve carbon gas vacuoles (cyanobacteria) Acidocalcisomes Acidocalcisomes are electron-dense acidic organelles rich in calcium and polyphosphate described in several parasites, algae, and slime molds. » Functions • storage of cations and phosphorus • polyphosphate metabolism • calcium homeostasis • maintenance of intracellular pH • osmoregulation. Acidocalcisomes Agrobacterium tumefaciens have flagella, it lives in the soil and accumulates in the rhizosphere around roots. Induces root tumors, and carries the distinct Ri (root-inducing) plasmid. Acidocalcisomes Agrobacterium tumefaciens attacks a broad spectrum of woody and herbaceous plants Euonymoum Brambles Grape Maple Rose Willow Cyanobacteria the blue-green algae Photosynthetic Chlorophyll a Phycobilins – Phycocyanin – Phycoerithrin Thylacoids Glycogen Cyanobacteria the blue-green algae Each cell is individual Joined by walls or mucilaginous sheaths Cyanobacteria the blue-green algae Specialized cells Necridia Hormogonia Cyanobacteria the blue-green algae Akinetes • Asexual propagules derived from vegetative cells. • Very thick walls. • Survive unfavorable conditions • Their thylakoids are not organized for photosynthesis. • Contain cyanophycin granules (Amino Acids) and lipids Cyanobacteria the blue-green algae Heterocyst • • • • • • N2 fixation N ammonium (NH4+) Thick cell wall Low oxygen concentration Photosystem II inactive Connected to vegetative cells by cell wall pores Symbiotic associations Azolla is a small water fern that assimilates nitrogen in symbiotic association with Anabaena azollae. The algal symbiont resides in the leaf cavity of Azolla in exchange for nutrients and carbon sources. Rhizobium Soil bacteria that fix nitrogen Endosymbiotic association with legumes root nodules Prochlorophytes prokaryotes that carry out oxygenic photosynthesis using chlorophylls a and b, but lack phycobili-proteins as light-harvesting pigments Prochloron Prochlorothrix Prochlorococcus Mycroplasma No cell walls Can assume various forms Spiroplasma citri causes stubborn disease in grapefruit and sweet oranges Phytoplasm They are mycoplasmalike organisms Parasites Passive or active Attack the sieve tube elements in the phloem Transmitted from plant to plant by insects Pseudomonas Xanthomonas Viruses Obligate intracellular parasites of • Plants • Animals • Fungi • Bacteria protein coat nucleic acid core DNA or RNA They cannot carry out metabolism Plant viruses 600 kinds of plant viruses 2000 plant diseases Common symptoms: Chlorotic or necrotic tissues Plant viruses DNA viruses Geminiviruses (groups of two) Badnaviruses (Bananas) Caulimoviruses (Cauliflower) Transmission from plant to plant Vectors with piercing and sucking mouthparts wounds From infected pollen to the ovule Inside the plant via plasmodesmata Phloem Movement proteins
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