e-Newsletter Since 1994 Primary Cells Primary Cell Specialists Media & Reagents Issue 6 Cell Biology Products Advances Continue in Vascular Smooth Muscle Cell Research Kikuchi et al. - Smooth Muscle Cell Medium About 30% of 300,000 annual human saphenous vein grafts fail within a year, and lmiting their injury during preparation could reduce this statistic. In Vascular Surgery, Kikuchi et al. report that crystal violet, routinely used in surgical marking pens, inhibits vein cell proliferation & migration, and increases cell death in grafts. Such dyes could inhibit vein graft healing and function, so other marking techniques should be considered. Smooth Muscle Cell Medium from Cell Applications, Inc was used to culture human cells isolated from leg bypass surgeries. Human Aortic Smooth Muscle Cells Ueda et al. - Rat Aortic Smooth Muscle Cells Most patients who die of abdominal aortic aneurysm (AAA) have no symptoms leading up to the event. Physicians strive to prevent aneurysmal rupture and improve prognosis by non-surgical treatments and drugs. AAA is characterized by activation of proinflammatory mediators, chronic inflammation and pathological tissue remodeling by proteolytic enzymes like matrix metalloproteinase (MMP)-9, which degrades extracellular matrix (ECM). Reporting in the journal PLoS ONE, Ueda et al. studied AAA pathogenesis mediated by Decorin, a small leucine-rich proteoglycan known to regulate vascular biology, immune responses, inflammation and collagen fibrillogenesis. The work, which utilized Rat Aortic Smooth Muscle Cells from CAI, suggests adventitial decorin in normal aorta may protect against AAA development, while macrophages expressing decorin in AAA walls may facilitate AAA progression by up-regulating MMP-9 secretion. González-Miguel et al. - Canine Aortic Smooth Muscle Cells Blood-borne parasitic pathogens release fibrinolysis-activating antigens that lead to plasmin overproduction, proliferation and migration of arterial wall cells, and ECM degradation. An in vitro culture model of endothelial and smooth muscle cells helped decipher mechanisms parasite antigens use to activate fibrinolysis. Also releasing data in PLoS ONE, the authors used Canine Aortic Endothelial and Smooth Muscle Cells, as well as Attachment Factor Solution and Trypsin/EDTA from Cell Applications. Late-Breaking Study World J Diabetes, 6: 662–672. Advanced glycation end-product expression is upregulated in the gastrointestinal tract of type 2 diabetic rats Sensory-motor abnormalities are common in the GI tract of diabetes mellitus patients. Previous work demonstrated changes in the morphological and biomechanical properties of the GI tract during diabetes. This study reveals that expression of advanced glycation end products (AGEs) and their receptor (RAGE) are up-regulated in the GI tract of GK diabetic rats and may contribute to GI dysfunction in type 2 diabetic patients. Receptor of advanced glycation end products immunestaining in villi (A) and crypt (B) of duodenum Anti-AGER Ab Hot Tech: Cell Bio in 3 Dimensions 1 1. Nonlinear 3D projection printing Concave hydrogel microstructures for long-term multicellular spheroid and embryoid body culture 2 3 from CAI was used in this study. 2. Label-Free 3D-Tissue Imaging 3rd Harmonic gen microscopy, non-fluorescent multi-photon technique combines label-free imaging & restriction of signal generation 3. In Vitro 3D Co-Culture Patterning Model Effect of Vascular Formed Endothelial Cell Network on the Invasive Capacity of Melanoma 4 4. 3D view of airways New technology finds a target for potential therapy of asthma and cardio obstructive pulmonary disease 5. Nondestructive 3-D imaging of cells with sound Emerging technique of "picosecond ultrasonics," a type of acoustic imaging, virtually slices tissues 6 5 6. Live-Cell 3D Imaging New and improved systems offer detailed, accurate spatial visualization of cells and their components CAI Support, Downloads and Literature Cell Types Poster Protocols, Instructions Product Flyers Brochure Trending: Cell Signal Transduction Pathways 1. Genetic 2. La Jolla Inst 3. U California Engineering News Allergy & Immunol San Diego 1. Deciphering Immune Cell Chatter 4. J Experimental Medicine Signal paths for Immune surveillance, environment response, homeostasis A Q Rat Astrocytes: RA 2. NFAT promotes T Cell exhaustion Transcription factor tires overstimulated CD8 cells & lowers immune response 3. G protein, RTK signaling Tuning cells by manipulating G proteins through receptor tyrosine kinases 4. Shear stress signals hematopoiesis Embryonic Blood flow triggers molecular cascade leading to stem cell birth Where can I find instructions for culturing the different cell types offered by CAI? F Cool Images from CAI On www.cellapplications.com, click the "Literature" tab near the upper right. Then, at the bottom of the Literature page, select and click the cell of your choice to open or download the cell type-specific “General Instructions for Culturing.” The protocols include important steps for safety, cell storage, reagent preparation, culturing, thawing, cell culture ware, incubation, plating, subculturing, differentiation and more. Rat Cardiomyocytes: RCm Current Promo: 20% Off Endothelial Cell Media Now Through July 31, 2015 Promotion Code ecmedia073115 (Mention when ordering) www.cellapplications.com News from the Cell Industry The CAI Book Club Sonic Hedgehog & Netrin-1 Blood Brain Barrier Immunology: A Short Course 7th Edition Complete Review Small Molecule Epothilone B “All the critical topics of modern immunology” Tight Junction Protein LSR “Clear and succinct yet comprehensive” Cell Applications, Inc. Modern text covers innate immunity, clinical aspects, and targeted antibody therapy Companion Website Interactive flashcards, figures, case-based material to help understand clinical applications June, 2015
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