e-Newsletter
Since 1994
Primary Cells
Primary Cell Specialists
Media & Reagents
Issue 6
Cell Biology Products
Advances Continue in Vascular Smooth Muscle Cell Research
Kikuchi et al. - Smooth Muscle Cell Medium
About 30% of 300,000 annual human saphenous vein grafts fail within a year, and lmiting their
injury during preparation could reduce this statistic. In Vascular Surgery, Kikuchi et al. report
that crystal violet, routinely used in surgical marking pens, inhibits vein cell proliferation &
migration, and increases cell death in grafts. Such dyes could inhibit vein graft healing and
function, so other marking techniques should be considered. Smooth Muscle Cell Medium from
Cell Applications, Inc was used to culture human cells isolated from leg bypass surgeries.
Human Aortic Smooth Muscle Cells
Ueda et al. - Rat Aortic Smooth Muscle Cells
Most patients who die of abdominal aortic aneurysm (AAA) have no symptoms leading up to the event. Physicians strive to prevent
aneurysmal rupture and improve prognosis by non-surgical treatments and drugs. AAA is characterized by activation of proinflammatory
mediators, chronic inflammation and pathological tissue remodeling by proteolytic enzymes like matrix metalloproteinase (MMP)-9, which
degrades extracellular matrix (ECM). Reporting in the journal PLoS ONE, Ueda et al. studied AAA pathogenesis mediated by Decorin, a
small leucine-rich proteoglycan known to regulate vascular biology, immune responses, inflammation and collagen fibrillogenesis. The
work, which utilized Rat Aortic Smooth Muscle Cells from CAI, suggests adventitial decorin in normal aorta may protect against AAA
development, while macrophages expressing decorin in AAA walls may facilitate AAA progression by up-regulating MMP-9 secretion.
González-Miguel et al. - Canine Aortic Smooth Muscle Cells
Blood-borne parasitic pathogens release fibrinolysis-activating antigens that lead to plasmin overproduction, proliferation and migration
of arterial wall cells, and ECM degradation. An in vitro culture model of endothelial and smooth muscle cells helped decipher
mechanisms parasite antigens use to activate fibrinolysis. Also releasing data in PLoS ONE, the authors used Canine Aortic Endothelial
and Smooth Muscle Cells, as well as Attachment Factor Solution and Trypsin/EDTA from Cell Applications.
Late-Breaking Study
World J Diabetes, 6: 662–672.
Advanced glycation end-product expression is upregulated
in the gastrointestinal tract of type 2 diabetic rats
Sensory-motor abnormalities are common in the GI tract of
diabetes mellitus patients. Previous work demonstrated
changes in the morphological and biomechanical properties of
the GI tract during diabetes. This study reveals that expression
of advanced glycation end products (AGEs) and their receptor
(RAGE) are up-regulated in the GI tract of GK diabetic rats and
may contribute to GI dysfunction in type 2 diabetic patients.
Receptor of advanced glycation end products immunestaining in villi (A) and crypt (B) of duodenum
Anti-AGER Ab
Hot Tech: Cell Bio in 3 Dimensions
1
1. Nonlinear 3D projection printing
Concave hydrogel microstructures for long-term
multicellular spheroid and embryoid body culture
2
3
from CAI was used in this study.
2. Label-Free 3D-Tissue Imaging
3rd Harmonic gen microscopy, non-fluorescent multi-photon technique
combines label-free imaging & restriction of signal generation
3. In Vitro 3D Co-Culture Patterning Model
Effect of Vascular Formed Endothelial Cell
Network on the Invasive Capacity of Melanoma
4
4. 3D view of airways
New technology finds a target for potential therapy of
asthma and cardio obstructive pulmonary disease
5. Nondestructive 3-D imaging of cells with sound
Emerging technique of "picosecond ultrasonics," a
type of acoustic imaging, virtually slices tissues
6
5
6. Live-Cell 3D Imaging
New and improved systems offer detailed, accurate
spatial visualization of cells and their components
CAI Support, Downloads and Literature
Cell Types Poster
Protocols, Instructions
Product Flyers
Brochure
Trending: Cell Signal Transduction Pathways
1. Genetic
2. La Jolla Inst 3. U California
Engineering News Allergy & Immunol San Diego
1. Deciphering
Immune Cell Chatter
4. J Experimental
Medicine
Signal paths for Immune surveillance,
environment response, homeostasis
A
Q
Rat Astrocytes: RA
2. NFAT promotes
T Cell exhaustion
Transcription factor tires overstimulated
CD8 cells & lowers immune response
3. G protein, RTK
signaling
Tuning cells by manipulating G proteins
through receptor tyrosine kinases
4. Shear stress signals
hematopoiesis
Embryonic Blood flow triggers molecular
cascade leading to stem cell birth
Where can I find instructions for culturing
the different cell types offered by CAI?
F
Cool Images from CAI
On www.cellapplications.com, click the "Literature"
tab near the upper right. Then, at the bottom of the
Literature page, select and click the cell of your
choice to open or download the cell type-specific
“General Instructions for Culturing.”
The protocols include important steps for safety, cell
storage, reagent preparation, culturing, thawing, cell
culture ware, incubation, plating, subculturing,
differentiation and more.
Rat Cardiomyocytes: RCm
Current Promo: 20% Off Endothelial Cell Media
Now Through
July 31, 2015
Promotion Code
ecmedia073115
(Mention when ordering)
www.cellapplications.com
News from the Cell Industry
The CAI Book Club
Sonic Hedgehog
& Netrin-1
Blood Brain Barrier
Immunology: A Short Course
7th Edition
Complete Review
Small Molecule
Epothilone B
“All the critical topics
of modern
immunology”
Tight Junction
Protein LSR
“Clear and succinct
yet comprehensive”
Cell Applications, Inc.
Modern text covers innate immunity, clinical
aspects, and targeted antibody therapy
Companion Website
Interactive flashcards, figures, case-based
material to help understand clinical applications
June, 2015