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TECHNICAL
BULLETIN
An evaluation of the effectiveness of sanitation procedures using an accelerated
hydrogen peroxide (Accel) disinfectant to reduce virus transmission via livestock
transport vehicles
(Schneider, P., Holtkamp, D., Ramirez, A., & Zhang, J., 2013 )
Abstract
Disease outbreaks within the swine industry can be devastating with mass economical costs. With the
constant threat of disease emergence, industry is looking to new chemical formulations, vetted
protocols, and scientific support as a front line of defence for their biosecurity. This study tests the
efficacy of a relatively new yet proven chemistry that is broadly used in healthcare facilities known as
Accelerated Hydrogen Peroxide® (AHP®), recognized for its superior cleaning ability and realistic contact
times, ensuring disinfection has taken place.
Background
Today's pork production industry is characterized by frequent movement of swine. With varying health
statuses of transported swine and the necessity to transport multiple groups in a short period of time,
make transportation vehicles a risk for spreading infectious diseases. Porcine reproductive and
respiratory syndrome virus (PRRSV) and transmissible gastroenteritis virus (TGEV) are two viruses with
large economic consequences that are capable of being transmitted by transport vehicles. Sanitation
procedures to decrease pathogen load in standard equipment used in swine transportation are not
always thoroughly cleaned between every transport event and can pose a risk to negative herds.
Study
This study compared the efficacy of Accel Concentrate disinfectant and Synergize disinfectant against
PRRSV and TGEV in model livestock trailers under conditions similar to those experienced in commercial
pork production. Additionally, this study used a bioassy to determine whether or not a sufficient
quantity of live PRRSV and TGEV were present in the model trailers to infect pigs when inoculated
intramuscularly (IM).
Model livestock trailers were contaminated with a slurry mixture containing PRRSV and TGEV and
allowed to sit in a 4° Celsius/39° Fahrenheit cooler for sixty minutes. The trailers were decontaminated
using a high-pressure washer with cold water for 90 seconds. Trailers were then disinfected using Accel
and/or Synergize. Four samples at 5 time points were collected for each trailer replicate. Time points
included pre-wash, immediately post-wash, and at 15, 30 and 60 minutes post disinfection. For each
replicate, samples from each time point were pooled and tested for the presence of PRRSV and TGEV.
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In order to test whether a sufficient quantity of live PRRSV and TGEV were present in model trailers to
infect pigs when inoculated intramuscularly, 24 10-week old PRRSV and TGEV negative pigs were
challenged with a minimum of 4mL of supernatant by IM injection and 7mL by oral gavage. Bioassy
groups were monitored for 14 days. Blood and fecal samples were collected and tested for the presence
of PRRSV or TGEV at days 7 and 14.
Results
Results from this study show that Accel disinfectant decreased the amount of PRRSV genomic copies on
board the contaminated model trailers quicker than Synergize disinfectant or no disinfectant when
paired with a 90-second cold-water, high-pressure wash. Both Accel and Synergize were effective at
inactivating PRRSV by 15 minutes post-application of disinfectant. A cold-water, high-pressure wash
alone was not effective at preventing inoculated bioassy pigs from becoming infected with PRRSV with
samples collected 15 minutes post-washing. The efficacy of Accel and Synergize against TGEV could not
be assessed due to the inability to maintain positive control groups throughout the course of the study.
However, no bioassy pigs tested positive for TGEV.
Conclusions
When time constraints prevent the gold standard of cleaning, disinfecting and drying of trailers, a
limited washing procedure combined with the proper use of a disinfectant, such as AHP is effective at
inactivating the presence of PRRSV in the environment.
Implications for AHP
1. AHP Disinfectants are One-Step Disinfectant Cleaners
 One-Step Disinfectant Cleaners provide you with added confidence – you clean while
you disinfect, and disinfect while you clean.
2. AHP Disinfectants have realistic contact times
 Short contact times ensure surfaces remain wet for the required contact time –
providing comfort and confidence that disinfection has occurred.
3. AHP Disinfectants provide the perfect balance between safety and efficacy
 All AHP products at in-use solutions do not require the use of Personal Protective
Equipment (PPE) without sacrificing germicidal potency against pathogens such as
PRRSV
4. AHP Disinfectants are compatible
 AHP formulations are tested to ensure compatibility that preserve your investments in
equipment, and building surfaces
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5. AHP Disinfectants are environmentally sustainable
 AHP’s active ingredient, hydrogen peroxide, breaks down into water and oxygen leaving

no active residues
AHP products have been inherently proven to be biodegradable indicating that properly
maintained septic systems and/or manure pits can handle normal wastes generated
from the use of AHP formulations
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