David W Reid, PhD Research Fellow Duke-NUS Medical School National University of Singapore 8 College Rd Singapore 169857 Email: [email protected] Tel: +1 541.206.5222 Citizenship: USA Education Duke University, Durham, NC 2014 PhD, Biochemistry Thesis: Segregation of protein synthesis between the cytoplasm and endoplasmic reticulum of eukaryotic cells Advisor: Christopher V Nicchitta University of Oregon, Eugene, OR 2009 BS with Honors in Biology, minor in Biochemistry Thesis: Identification of Drosophila genes that modify the activity of Helicobacter pylori CagA Advisor: Karen J Guillemin Research experience Research Fellow, Duke-NUS Medical School, National University of Singapore 2014- Advisor: Shirish Shenolikar Investigated the changes in protein synthesis that are associated with disruptions in proteostasis, particularly in models relevant to Parkinson’s Disease. Graduate student, Department of Biochemistry, Duke University 2010-2014 Advisor: Christopher V Nicchitta Integrated biochemical and computational tools to probe the role of mRNA localization in translational regulation and cell stress responses Rotation student, Department of Biochemistry, Duke University 2009 Advisor: Lorena S Beese Purified and crystallized farnesyltransferases from pathogenic fungi, obtaining highresolution structures Advisor: Jack D Keene Developed computational techniques to analyze high-throughput sequencing data for RNA binding protein footprinting experiments Research Assistant, Institute of Molecular Biology, University of Oregon Advisor: Karen J Guillemin Developed and employed a Drosophila model of Helicobacter pylori pathogenesis, focusing on the effect of the pathogenicity factor CagA upon cell junction integrity Page 1 of 4 2006-2009 David W Reid – Curriculum vitae Year Cited 2015 1 Comment on "Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling" 2015 1 The Unfolded Protein Response Triggers Selective mRNA Release from the Endoplasmic Reticulum 2014 9 A novel ribosomopathy caused by dysfunction of RPL10 disrupts neurodevelopment and causes X-linked microcephaly in humans. 2014 6 De novo translation initiation on membrane-bound ribosomes as a mechanism for localization of cytosolic protein mRNAs to the endoplasmic reticulum 2014 2 Multifunctional roles for the protein translocation machinery in RNA anchoring to the endoplasmic reticulum 2014 3 Premature translational termination products are rapidly degraded substrates for MHC Class I presentation 2012 3 The enduring enigma of nuclear translation 2012 5 Primary role for endoplasmic reticulum-bound ribosomes in cellular translation identified by ribosome profiling 2012 60 Hierarchical regulation of mRNA localization to the endoplasmic reticulum 2011 30 Integrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA splicing and mRNA stability 2011 226 Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila 2012 5 Publications: 12 (7 first-author) | Total citations: 341| h-index: 5 Simple and inexpensive ribosome profiling analysis of mRNA translation Reid DW, Shenolikar S, Nicchitta CV In revision, Methods Diversity and selectivity in mRNA Translation on the Endoplasmic Reticulum Reid DW, Nicchitta CV Nature Reviews Molecular Cell Biology. 2015 Apr;16(4):221-231 Featured Article, April 2015 issue Reid DW, Nicchitta CV Science. 348, 1217 Reid DW, Chen Q, Ling TS, Shenolikar S, Nicchitta CV Cell. 158(6):1362-74 Featured in Nature Chemical Biology Brooks SS, Wall AL, Golzio C, Reid DW, Kondyles A, Willer JR, Botti C, Nicchitta CV, Katsanis N, Davis EE Genetics. 198(2):723-33 Jagannathan S, Reid DW, Cox AH, Nicchitta CV RNA. 2014(20):1489-98 Jagannathan S, Hsu JC, Reid DW, Chen Q, Thompson WJ, Moseley AM, Nicchitta CV Journal of Biological Chemistry. 289(37):25907-24 Lacsina JR, Marks OA, Liu X, Reid DW, Jagannathan S, Nicchitta CV PLoS ONE. 7(12):e51968 Reid DW, Nicchitta CV Journal of Cell Biology. 197(1):7-9 Reid DW, Nicchitta CV Journal of Biological Chemistry. 287(8):5518-27. Featured in F1000 Chen Q*, Jagannathan S*, Reid DW*, Zheng T, Nicchitta CV Molecular Biology of the Cell. 22(14):2646-58 Mukherjee N, Nusbaum J, Corcoran D, Reid DW, Georgiev S, Hafner M, Ascano M, Tuschl T, Ohler U, Keene JD Molecular Cell. 43(3):327-39 Reid DW*, Muyskens JB*, Neal JT*, Gaddini GW, Cho LY, Wandler AM, Botham CM, Guillemin K. Frontiers in Microbiology. 2012(2):24 Page 2 of 4 David W Reid – Curriculum vitae Presentations Gordon Conference: Translational Machinery in Health and Disease, Ventura, CA The unfolded protein response triggers selective mRNA release from the ER 2015 Duke-NUS Annual Retreat, Singapore Selected talk: Release of mRNAs from the ER as a stress response mechanism Awarded best selected presentation 2014 ASBMB: The Endoplasmic Reticulum in Health and Disease, Warrenton, VA Selected talk: A primary role for ER-bound ribosomes in cellular protein synthesis 2013 Triangle Center for RNA Biology, Chapel Hill, NC A primary role for ER-bound ribosomes in cellular protein synthesis ASCB Annual Meeting, Denver, CO A primary role for the endoplasmic reticulum in protein synthesis 2012 2011 Awards Barry M Goldwater Scholarship, University of Oregon nominee 2008 William L Martin Scholarship 2006 Dean’s Scholarship, University of Oregon 2006 Volunteering and community involvement Board of Trustees Member, Duke University, Facilities & Environment Oversaw University strategy, planning, and operations for construction and environmental initiatives Graduate student representative, Duke Committee on Facilities & Environment Advised the Board of Trustees on construction, transportation, and finances. Particularly active in alternative transportation Demonstrator, Science Night, Scroggs Elementary, Carrboro, NC Demonstrated properties and principles of acids and bases to third through fifth graders at an annual science night Advisor, Little Flower High School, Philadelphia, PA Advised high school students interested in science about education and careers Member, Durham Bike Co-op, Durham, NC Helped community members build and repair their own bikes Executive Board Member, National Intercollegiate Running Club Association Led club recruitment, marketing, and competition efforts for the national running club organization, helping the group to double in size Coordinator, University of Oregon Running Club Directed fund raising, budgeting, team selection, and travel, culminating in a national championship Page 3 of 4 2013-2014 2012-2013 2012-2014 2011-2012 2011-2014 2008-2010 2007-2009 David W Reid – Curriculum vitae Technical expertise Deep sequencing techniques: Ribosome profiling, mRNA-seq, small RNA-seq Computational analysis: Python, R, LINUX, Bowtie, standard deep sequencing toolsets Biochemical techniques: Ultracentrifugation, metabolic labelling, cell culture, PCR Model organisms: Mammalian cell culture (including primary and neurons), fruit fly, zebrafish Teaching and mentoring High school student mentor Hosted and advised a high school student over two summers, introducing her to biochemical and computational techniques and career choices 2013-2014 Teaching Assistant, Department of Biochemistry, Duke University Course: Biochemistry II Advisor: Michael D Been Designed and presented weekly lectures to reinforce material 2011 Teaching Assistant, Department of Biology, University of Oregon Course: Microbiology Laboratory Advisor: Alan J Kelly Helped students perform and interpret experiments, developed new curricula 2009 References Shirish Shenolikar – Professor, Duke-NUS Graduate Medical School Post-doctoral advisor Phone: +65.6516.2588 Email: [email protected] Christopher Nicchitta – Professor of Cell Biology and Biochemistry, Duke University Graduate advisor Phone: 919.684.8948 Email: [email protected] Karen Guillemin – Professor of Biology, University of Oregon Undergraduate advisor Phone: 541.346.5999 Email: [email protected] Jack Keene – Professor of Molecular Genetics and Microbiology, Duke University Collaborator, thesis committee member Phone: 919.684.5138 Email: [email protected] Page 4 of 4
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