1. technology and computing

Bioseparations
PolyBind™ Affinity Resins for Purification/Enrichment
Achieve Superior Purification/Enrichment of
your Target with the New Range of PolyBind
BioBead™ Affinity Resins.
PolyBind™ is a family of disposable affinity resins,
easily customized with a wide range of scaffolds
and ligands.
Application Highlights:
• Single-use economy and multi-cycle durability.
• Faster production turnaround times; saves time
in cleanup and validation.
• Customizable—Displays one or more polypeptide
ligands; improved binding kinetics and affinity.
• Surface display of the protein ligand means faster
process kinetics.
• Adaptable to multiple formats: e.g. cross-flow
filtration, packed bed and expanded bed columns.
• Suitable for protein, nucleic acid, or whole cell
enrichment/purification.
Bioseparations
What is the PolyBatics™ Platform?
Platform Applications
The range of proteins that can be displayed on PolyBind
Single-step Production of Protein-Displaying
BioBeads™
BioBeads™ creates the potential for plug and play
PolyBatics’ BioBeads™ are manufactured via a one-step
company has demonstrated the platform’s potential in
in vivo process that produces proteins immobilized on
industrial biotechnology, bioremediation, API produc-
biopolymer beads. This technology platform eliminates
tion and vaccine delivery. As we take this game changing
the need for secondary cross-linking steps to bind your
technology to users around the world, new applications
protein to a solid support.
are being discovered.
PolyBatics scientists have engineered a specific enzyme
Compelling Features of the PolyBatics’ Platform
that enables the production of functionalized biopolymer
•R
apid prototyping enables fast screening and evaluation
solutions across a number of applications. To date the
beads, BioBeads™. The gene(s) for desired proteinmediated functions e.g. ligand, enzyme, reporter molecule, etc. are linked to the gene for the bead-forming
of possible functional bead constructs.
•D
isplayed proteins are consistently oriented in the same
direction on the bead surface ensuring optimal perfor-
enzyme and the resulting hybrid gene fusion is inserted
mance per unit of protein.
into the production host. The result is a unique biological
manufacturing system that produces polymer BioBeads™
•M
ultiple copies of a functional domain or different func-
displaying covalently attached, fully-functional proteins on
tions may be combined on a single bead for optimal
their surfaces.
performance.
Traditional methods of protein immobilization result in
• The bead core can be loaded with drug or dye and
random orientation display of the functional protein or
delivered to specific cells via targeting proteins on the
ligand on the surface of a solid support, possibly occluding
core surface.
the functional domain, e.g. binding or catalytic site, and
compromising performance. PolyBatics’ protein display
•B
eads self-assemble to produce consistent, uniform,
high-density display on their surfaces.
is the result of hybrid gene expression in the host cell, so
all of the functional domains are displayed in their proper
orientation, properly folded every time.
Hybrid Gene Expression and
Self-Assembly of BioBeads™
Conventional Multi-Step Process
Chemicals
Solvents
Energy
Bacterial Cell
Cross-link
Polymer
zz domain displayed
at polymer granule
ZZ domain
Linker
Synthase
Functional Protein:
enzyme, ligand,
antigen, etc.
Synthase
Antibody
Beads
Solvents
Energy
Solvents
Energy
Wash
Protein
Functional Bead
The conventional process requires several steps: resin
production, ligand production, cross-linking (toxic), and excess
cross-linker removal. Random orientation of the ligand on the
resin can affect performance.
Bioseparations
PolyBind™-Superior Separation Performance
PolyBind™ versus Competitors
100
Protein Purification
Market Need: Demand for Higher Efficiency and
75
• Growing protein therapeutics pipeline: Monoclonal
antibodies (over 290 in development), Fc fusion
therapeutics, other protein drugs in development.
• Higher titers from cell line production (>10g/L): process
bottleneck migrating downstream.
mg/ml
Lower Cost Methods
50
25
0
PolyBind BioBead
Competitor 1
Competitor 2
Binding capacity from eluted human IgG
• Smaller production runs, use of disposables, multi-drug
facilities: process flexibility required.
Relative Binding Capacity
The Superior Solution: PolyBind™
• PolyBatics’ technology enables ultra low-cost, in vivo
1 2
3
4 5
6
7
8 9 10
production of a wide variety of protein-displaying
2. Pure IgG (5mg/ml)
BioBeads™ for bioseparations uses.
3. Wild Type Unbound
• Displayed proteins are natively folded, functionally
4. Wild Type Elution
oriented on the BioBead™ surface.
5. Pure IgG (5mg/ml)
• More rapid binding: Greater than 90% of available IgG
6. GE MabSelect Unbound
within two minutes (batch adsorption).
7. GE MabSelect Elution
• Beads are orders of magnitude smaller (300-400nm)
than agarose, providing larger surface binding area.
• Higher per-unit bead capacity than traditional resins.
PolyBatics’ One-Step Process
1. BioLabs Ladder
8. Pure IgG (5mg/ml)
This gel demonstrates the relative
IgG purification by PolyBind-Z™
versus wild type BioBeads™ and
GE MabSelect Sure.
9. PolyBind-Z™ Unbound
10. PolyBind-Z™ Elution
Applications
separations
Nutrients
Buffers
Cell
microbe
Functional Bead
The PolyBatics’ one-step process occurs in vivo resulting
in a superior resin with optimal ligand orientation at a
fraction of the cost.
protein
production
enzyme
delivery
drug
delivery
vaccines
diagnostics
Filling a Critical Gap in Disposable Processing
PolyBind™ is cost-effective by orders of magnitude and
addresses the growing industry trend towards disposable
manufacturing systems. Responding to increased CMO
outsourcing, smaller production runs, the emergence
of biosimilars, and flexible manufacturing systems, the
PolyBind™ solution offers the convenience of disposability
without compromising quality or performance. Suitable
for single use, but robust enough for multiple bind:elute
cycles, PolyBind is designed to improve process economics
in the capture and purification of therapeutic targets.
Other PolyBind™ Targets
In addition to PolyBatics’ work in protein purification,
PolyBind BioBeads™ have been applied to the separation
and enrichment of other important biological targets.
Nucleic Acids
PolyBind BioBeads™ are being developed for enrichment
of methylated DNA for use in epigenetics research. Using
our magnetic PolyBind™ methyl binding peptide (MBP)
displaying beads, methylated DNA can be enriched for
subsequent bisulfite analysis.
Using streptavidin-displaying magnetic PolyBind
BioBeads™, any biotinylated capture probe can be linked
and separated from a complex. Examples are for the
isolation of specific histones or nucleic acid sequences for
PCR amplification of the targeted sequence.
Cell Enrichment
PolyBind BioBeads™ provide excellent separation and
isolation of pure, viable and functional cells, resulting in
higher yields. The consistent orientation of the BioBead™
surface ligand display ensures that cells are not exposed
to process stress. Whether you’re pursuing positive Isolation, negative isolation or depletion of your cells, PolyBind
magnetic BioBeads™ provide the ideal solution.
Bio Commerce Centre
PO Box 1210
Palmerston North 4440
New Zealand
www.polybatics.com
US Phone +1 (415) 287-3821
NZ Phone +64 (6) 352 0200