1. No category

1. Describe the three steps and molecules
involved in the semi-conservative replication
of DNA (be sure to include the enzymes
involved):
 “unzipping”
 Complementary Base Pairing
 Joining of Adjacent Nucleotides
2.
Describe the purpose of DNA replication.
Identify the site of DNA replication within the cell.
Define recombinant DNA (rDNA).
3. Identify the roles of DNA, mRNA, tRNA, and
ribosomes in the process of transcription and
translation including:
 Initiation
 Elongation
 Termination
4. Complete the following chart:
Template
strand of DNA
mRNA (codons)
tRNA
(anticodons)
Amino acid
sequence
TAC GGT CCC CGC ATT
5.
An error occurred when DNA was being replicated. The
original DNA sequence was
TAC ATA GGG and a “T” was placed in the sixth nucleotide
position instead of an “A”.
a) What kind of genetic mutation is this?
HINT: you
must convert the DNA sequence to a mRNA sequence in order to
read the codon
chart._________________________________
b) What is the result of this type of mutation?
6. Sketch the cell membrane – including
phospholipids, proteins, glycoproteins,
glycolipids, carbohydrates, and cholesterol
 Identify the hydrophobic and hydrophilic regions
of the phospholipid bilayer.
 Explain why the cell membrane is described as
“selectively permeable”
7.
 What is diffusion?
 Give an example of diffusion in the body.
 Provide 3 factors that affect the rate of diffusion
across a cell membrane.
8.
In an experiment, the bottom of a thistle tube is covered with a membrane
permeable only to water. The thistle tube is filled with a 10% sucrose
solution and placed in a beaker containing a 5% sucrose solution. What will
happen to the solution in the tube after one hour?
A. The concentration of sucrose will decrease and become isotonic to the
solution in the beaker.
B. The concentration of sucrose will increase and become isotonic to the
solution in the beaker.
C. The concentration of sucrose will decrease and become hypotonic to the
solution in the beaker.
D. The concentration of sucrose will increase and become hypotonic to the
solution in the beaker.
On the diagram above – label HYPERTONIC and HYPOTONIC
9.
Label the following diagram of the Lock and Key
Model of Enzymatic Action.
What is activation energy? How do enzymes affect it?
Explain how the following affect enzyme activity:
 High temperatures
 Low temperatures
 Exposing the enzyme to lead
 Changing the pH from the optimal pH