How to use the API Strips 2012 Quiz: What can you remember about API system? (Answers are listed at the end). 1. What type of bacteria may be identified using the API 20E strip? a. b. c. d. Enterobacteriaceae Staphylococci Anaerobes All of the above 2. What two tests are usually performed prior to API 20 E strip to confirm the microbe is a member of the Enterobacteriaceae? a. b. c. d. Oxidase and catalase Gram’s staining and oxidase Oxidase and coagulase Catalase and coagulase 3. What is the purpose of filling the bottom tray of the API strip holder with water? a. b. c. d. To dissolve reagents To provide a humidified chamber for the biochemical tests The bottom tray of the API strip holder does not need to be filled with water None of the above How to use the API Strips 2012 4. Why do some of the biochemical tests require oil to be added to the cupule? a. b. c. d. Introduces anaerobic conditions Stops the end gaseous products of certain reactions to be released To prevent unwanted reactions All of the above 5. In what conditions are the API 20E strips incubated? a. b. c. d. 37°C for 18-24h aerobically 32°C for 24-48h aerobically 37°C for 18-24h anaerobically 22°C for 2-3 days aerobically 6. What does the API abbreviation stand for? a. b. c. d. Advance Profile Index Applied Profile Information Analytical Profile Index None of the above 7. What may the negative result for glucose test on the API 20E strip indicate? a. b. c. d. The tested organism did not grow in this microtube The tested bacterium is not from the Enterobacteriaceae family The tested bacterium belongs to Enterobacteriaceae Both a and b are correct 8. How would you interpret the result for the IND test on the following API 20E strip? a. Positive (+) b. Negative (-) c. Invalid (required to be repeated) How to use the API Strips 2012 9. How would you interpret the result for the VP tests on the following API 20E strips? 1 2 a. b. c. d. Both Positive (+) 1 Positive and 2 Negative (-) Both Negative None of the above 10. How would you interpret the result for the GEL test on the following API 20E strip? a. Positive (+) b. Negative (-) c. Invalid (requires to be repeated) How to use the API Strips 2012 11. How would you interpret the result for the ODC tests on the following API 20E strip? a. Negative (-) b. Positive (+) c. Invalid (requires to be repeated) 12. Some of the tubes of the API 20E strip need to be filled with the bacterial suspension right to the very top end including the cupule area. Which are these tubes? a. b. c. d. CIT, H2S, VP ADH, CIT, VP ADH, LDC, ODC CIT, VP, GEL 13. The following tubes (ADH, LDC, ODC, H2S, URE) are underlined. Why? a. These tubes are required to be filled with the bacterial suspension at the top area (cupule) b. These tubes are required to have oil added at the top (cupule) c. The tests within these tubes require anaerobic conditions d. Both b and c are correct 14. What colour change may indicate a positive (+) reaction for the glucose test on the API 20E strip? a. b. c. d. Blue to orange Blue to yellow Yellow to blue Green to blue How to use the API Strips 2012 15. The results for the API 20E strip test are recorded on the results sheet and the final profile number is obtained. How many digits does that profile number have? a. b. c. d. 7 5 6 8 ANSWERS 1. What type of bacteria may be identified using the API 20E strip? e. f. g. h. Enterobacteriaceae (correct answer) Staphylococci Anaerobes All of the above 2. What two tests are usually performed prior to API 20 E strip to confirm the microbe is a member of the Enterobacteriaceae? e. f. g. h. Oxidase and catalase Gram’s staining and oxidase (correct answer) Oxidase and coagulase Catalase and coagulase 3. What is the purpose of filling the bottom tray of the API strip holder with water? e. To dissolve reagents f. To provide a humidified chamber for the biochemical tests (correct answer) g. The bottom tray of the API strip holder does not need to be filled with water h. None of the above 4. Why do some of the biochemical tests require oil to be added to the cupule? e. f. g. h. Introduces anaerobic conditions Stops the end gaseous products of certain reactions to be released To prevent unwanted reactions All of the above (correct answer) 5. In what conditions are the API 20E strips incubated? How to use the API Strips 2012 e. f. g. h. 37°C for 18-24h aerobically (correct answer) 32°C for 24-48h aerobically 37°C for 18-24h anaerobically 22°C for 2-3 days aerobically 6. What does the API abbreviation stand for? e. f. g. h. Advance Profile Index Applied Profile Information Analytical Profile Index (correct answer) None of the above 7. What may the negative result for glucose test on the API 20E strip indicate? e. f. g. h. The tested organism did not grow in this microtube The tested bacterium is not from the Enterobacteriaceae family The tested bacterium belongs to Enterobacteriaceae Both a and b are correct (correct answer) 8. How would you interpret the result for the IND test on the following API 20E strip? d. Positive (+) (correct answer) e. Negative (-) f. Invalid (required to be repeated) 9. How would you interpret the result for the VP tests on the following API 20E strips? 1 How to use the API Strips 2012 2 e. f. g. h. Both Positive (+) 1 Positive and 2 Negative (-)(correct answer) Both Negative None of the above 10. How would you interpret the result for the GEL test on the following API 20E strip? d. Positive (+) (correct answer) e. Negative (-) f. Invalid (requires to be repeated) 11. How would you interpret the result for the ODC tests on the following API 20E strip? How to use the API Strips 2012 d. Negative (-) e. Positive (+) (correct answer) f. Invalid (requires to be repeated) 12. Some of the tubes of the API 20E strip need to be filled with the bacterial suspension right to the very top end including the cupule area. Which are these tubes? e. f. g. h. CIT, H2S, VP ADH, CIT, VP ADH, LDC, ODC CIT, VP, GEL (correct answer) 13. The following tubes (ADH, LDC, ODC, H2S, URE) are underlined. Why? e. These tubes are required to be filled with the bacterial suspension at the top area (cupule) f. These tubes are required to have oil added at the top (cupule) g. The tests within these tubes require anaerobic conditions h. Both b and c are correct (correct answer) 14. What colour change may indicate a positive (+) reaction for the glucose test on the API 20E strip? e. f. g. h. Blue to orange Blue to yellow (correct answer) Yellow to blue Green to blue 15. The results for the API 20E strip test are recorded on the results sheet and the final profile number is obtained. How many digits does that profile number have? How to use the API Strips 2012 e. f. g. h. 7 (correct answer) 5 6 8