SIF Powder FaSSIF-V2 How To Use Document Name: SIF Powder FaSSIF-V2 How To Use Document Version: 1.0 This User Guide explains how to make 1 Litre of FaSSIF-V2. For any other volume use the Calculator on our website http://biorelevant.com/fassif-v2/how-to-make. How to make 1 litre of FaSSIF-V2 Step 1 Preparation of maleate buffer for FaSSIF-V2 (1 Litre): Dissolve: 1.39 g NaOH (pellets) 2.23 g of maleic acid 4.01 g of NaCl in 0.9 L of purified water Adjust the pH to 6.5 with either 1 N NaOH or 1 N HCl and make up to volume (1 L) with purified water Step 2 To make 1 litre of FaSSIF-V2: • Add 1.79 g of SIF Powder FaSSIF-V2 to about 500 mL of buffer at room temperature • Stir until SIF Powder has dissolved • Make up to volume (1 L) with the buffer Let FaSSIF stand for 1 hour. FaSSIF-V2 is ready for use. Storage and Handling • Refrigerate SIF Powder FaSSIF-V2 bottle at 2-8°C • Before opening, equilibrate SIF Powder FaSSIF-V2 bottle to room temperature • Use the FaSSIF-V2 you have made within 48 hours at room temperature and 24 hours at 37°C Drug Analysis *Use HPLC to measure drug content* Any questions? Contact us: [email protected] Instant Powders for the preparation of biorelevant media are covered by PCT/EP2006/010809. b i o r e l e v a n t . c o m , U n i t 2 0 , C a p i t a l B u s i n e s s C e n t r e , 2 2 C a r l t o n R d . , C R 2 0 B S , U n i t e d K i n g d o m Tel: +44 (0)20 7609 3499 E-Mail: [email protected] Webpage: http://www.biorelevant.com © 2013 biorelevant.com Page 1 of 1
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