MSDS# 7898 COVER SHEET 8404902 Whole Cell Stain Toolbox Kit Component # 8403202 1860101 1860288 1860289 1860290 1860291 Description Cellomics Whole Cell Stain Hoechst Dye Wash Buffer 10X Wash Buffer II Permeabilization Buffer 10X Blocking Buffer 10X Life Science Research PO Box 117 Rockford, IL (815) 968-0747 Pierce Biotechnology Inc. 3747 N. Meridian Road 61105 (815) 968-7316 fax www.thermo.com % $"#$ $1 0 4+5&$ $$ 6 ! 0 4+5&$ $$ 6 ! 4 4+5&$ $$ 6 ! 9 4+5&$ $$ 6 ! '48503 ) 42$ ! )0 42$ ! , 42$ ! 7#2## $5&@ 2 5 & "#$ %& %!'!() *+$,-. / !!)) !)!) / "#$ %& %!'!() *+$,-. / !!)) !)!) 00448 5 4+5! 50 67 ) ! ! 012 , !""#$#%&"" '-. '( )( $"$#!&*++ ! &3 0 ( : 2 ; ? ? : 2 ; ? ? 0-.-,-0 . 14<0 $1 #9 044 0- 1 '23 ! 7*4-489 7/33:3-**-"7"-'4! 7 5! & $ &! "# 6 ;'<7< 6 ## =*!>! 3 !- !-&! ) )0 , 0 , ) )0 4+5&$ $$ 6 ! 4+5&$ $$ 6 ! 4+5&$ $$ 6 ! &!*+$ # &! 8 : 4+5&$ $$ 6 ! 4+5&$ $$ 6 ! 3$+3$" .$* %'()*+$,-.=>?))555!#!# %&-!))4!0 * =>?) 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(.,>,?A'.& >< :6-1(1"+(6%(;(6:67<+7+((+6+%7/6;16(/-+ 56%51:%76+7/61(+65+:/6;:6-11(+618171+: 11:*61%7+7=+(%66 1 6*27 H 9 1 " ! :9 26*27 (3&%3&$ 24627B7 46!7B6##0 .#&0 C4B7 D4B7 :.E4 6:E7B6##0 .#&0 467B7 (3&%3&$ ! (."%&'()!*+>,?A''444 " #$ + ''7/. (.,>,?A'.& =) 9 34 (3&%3&$ 4 70@@.#. /0.# 44 : " -./ .4/48 / /.4 9 2 -.1.21/. 15 (3&%3&$ ! (."%&'()!*+>,?A''444 " #$ + ''7/. (.,>,?A'.& Part of Thermo Fisher Scientific Wash Buffer, 10X Dulbecco's PBS 2. Material Safety Data Sheet 1. Product and company identification : Wash Buffer, 10X Dulbecco's PBS : Env Sensors Subassembly; Wash Buffer 10X Dulbecco's PBS Manufacturer : Thermo Fisher Scientific : Thermo Fisher Scientific Pierce Biotechnology Pierce Biotechnology P.O. Box 117 P.O. Box 117 Rockford, IL 61105 Rockford, IL 61105 United States United States 815.968.0747 or 815.968.0747 800.874.3723 800.874.3723 Product name Synonym Supplier Product No. MSDS # Validation date Print date Responsible name In case of emergency 2. : : : : : : 1860288 1862330 1901715 8408200 7512 7/26/2010. 7/26/2010. MSDS Specialist CHEMTREC: Use of 800.424.9300 Substance/Preparation OUTSIDE US: 202.483.7616 Emergency overview Ingestion Skin Eyes Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. : Liquid. : Odorless. : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). : WARNING ! CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. Irritating to eyes, respiratory system and skin. Avoid exposure - obtain special instructions before use. Do not breathe vapor or mist. Avoid contact with eyes, skin and clothing. Contains material that can cause target organ damage. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. (815) 968-0747 (815) 968-7316 Fax : Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments. Classification : Not classified. See toxicological information (section 11) 3. Composition/information on ingredients United States Name Sodium Chloride Sodium Phosphate, Dibasic Europe Substance/preparation Ingredient name CAS number 7647-14-5 7558-79-4 % 7 - 10 1-3 : Preparation CAS number 7558-79-4 % EC number 1-3 231-448-7 Classification [1] Xi; R36/37/38 There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit Occupational exposure limits, if available, are listed in section 8. 1/10 Rockford, IL 61105 Medical conditions aggravated by overexposure Sodium Phosphate, Dibasic 7/26/2010. 3747 N. Meridian Rd. PO Box 117 No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. Contains material which causes damage to the following organs: mucous membranes, skin. Contains material which may cause damage to the following organs: eyes, stomach. : Adverse symptoms may include the following: respiratory tract irritation coughing : No specific data. : Adverse symptoms may include the following: irritation redness : Adverse symptoms may include the following: pain or irritation watering redness Inhalation : Eye contact. Inhalation. Ingestion. Routes of entry Potential acute health effects Inhalation : Irritating to respiratory system. : No known significant effects or critical hazards. Ingestion : Irritating to skin. Skin : Irritating to eyes. Eyes Potential chronic health effects Chronic effects : Contains material that can cause target organ damage. Carcinogenicity : No known significant effects or critical hazards. Biosciences Division Pierce : : : : : Over-exposure signs/symptoms Hazards identification Physical state Odor OSHA/HCS status Hazards identification Mutagenicity Teratogenicity Developmental effects Fertility effects Target organs www.thermo.com/pierce 2/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce Wash Buffer, 10X Dulbecco's PBS Wash Buffer, 10X Dulbecco's PBS 4. 6. First aid measures : Move exposed person to fresh air. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. Keep person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Inhalation : Wash out mouth with water. Remove dentures if any. Move exposed person to fresh air. Keep person warm and at rest. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Ingestion Skin contact Eye contact Protection of first-aiders : Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing before reuse. Clean shoes thoroughly before reuse. : Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention. : No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Accidental release measures Personal precautions : No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). Environmental precautions : Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). : Stop leak if without risk. Move containers from spill area. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see section 13). Dispose of via a licensed waste disposal contractor. Contaminated absorbent material may pose the same hazard as the spilled product. Note: see section 1 for emergency contact information and section 13 for waste disposal. Large spill : Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble or absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Small spill 7. Handling and storage Handling : Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. : In a fire or if heated, a pressure increase will occur and the container may burst. Storage : Use an extinguishing agent suitable for the surrounding fire. : None known. : Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. : Decomposition products may include the following materials: phosphorus oxides halogenated compounds metal oxide/oxides : Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. 8. See section 11 for more detailed information on health effects and symptoms. 5. Fire-fighting measures Flammability of the product Extinguishing media Suitable Not suitable Special exposure hazards Hazardous combustion products Special protective equipment for fire-fighters : Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. 3/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce Exposure controls/personal protection Europe No exposure limit value known. Consult local authorities for acceptable exposure limits. Recommended monitoring : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation procedures or other control measures and/or the necessity to use respiratory protective equipment. Engineering measures : Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. 4/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce Wash Buffer, 10X Dulbecco's PBS Wash Buffer, 10X Dulbecco's PBS 8. 11 . Toxicological information Exposure controls/personal protection Hygiene measures Personal protection Respiratory Hands Eyes Skin Environmental exposure controls 9. : Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. : Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. : Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. : Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. : Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. : Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Physical and chemical properties : : : : : Physical state Color Odor pH Solubility United States Acute toxicity Product/ingredient name sodium phosphate, dibasic Liquid. Colorless. Odorless. 6.6 to 7 Easily soluble in the following materials: cold water and hot water. sodium chloride Conclusion/Summary Chronic toxicity Conclusion/Summary Carcinogenicity Conclusion/Summary Classification Product/ingredient name sodium chloride sodium phosphate, dibasic Mutagenicity Product/ingredient name sodium chloride 10 . Stability and reactivity Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Result LD50 Oral LDLo Intraperitoneal LDLo Intramuscular LDLo Subcutaneous LD50 Dermal LD50 Intraperitoneal LD50 Oral LDLo Intraperitoneal LDLo Subcutaneous LC50 Inhalation Dusts and mists : Not available. Species Rat Rat Dose 17000 mg/kg 1 g/kg Exposure - Rat 1 g/kg - Rat 1 g/kg - Rabbit Rat 10000 mg/kg 2600 mg/kg - Rat Rat 3000 mg/kg 3.72 g/kg - Rat 3500 mg/kg - Rat >42 gm/m3 1 hours : Not available. : Not available. ACGIH - IARC - Test DNA Inhibition Unscheduled DNA Synthesis (UDS) Other Mutation Test Systems Cytogenetic Analysis DNA Damage : The product is stable. Under normal conditions of storage and use, hazardous polymerization will not occur. : No specific data. : No specific data. : Under normal conditions of storage and use, hazardous decomposition products should not be produced. - : Will not occur. Micronucleus Test DNA Damage Cytogenetic Analysis Cytogenetic Analysis 5/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce EPA - NIOSH None. None. NTP - Experiment Mammalian-Human; Somatic Mammalian-Animal Result Positive Mammalian-Animal Positive Mammalian-Animal Mammalian-Animal; Somatic Mammalian-Animal; Somatic Mammalian-Animal; Somatic Mammalian-Animal; Somatic Mammalian-Animal; Somatic Mammalian-Animal; Somatic Positive Positive OSHA None. None. Positive Positive Positive Positive Positive Positive 6/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce Wash Buffer, 10X Dulbecco's PBS Wash Buffer, 10X Dulbecco's PBS 11 . Toxicological information 11 . Toxicological information Conclusion/Summary Teratogenicity Product/ingredient name sodium chloride Conclusion/Summary Reproductive toxicity Product/ingredient name sodium chloride : Not available. Europe Result Positive Intraperitoneal Positive Subcutaneous Positive Subcutaneous Positive Subcutaneous Not available. : Maternal toxicity - - Fertility Species Rat Dose 1710 mg/kg Mouse 2500 mg/kg Mouse 1900 mg/kg Mouse 1900 mg/kg Exposure 13 days During Pregnancy 10 days During Pregnancy 10 days During Pregnancy 11 days During Pregnancy Species Dose - Development toxin - Rat Intraperitoneal: Positive - Mouse 10 g/kg During Exposure - 13440 mg/kg During Positive - Positive - - Positive - - - - - Mammal - Unreported: 6 g/kg species unspecified Parenteral: Rat 10 mg/kg Mammal - Unreported: 480 species unspecified mg/kg Rat Oral: 145 g/kg During - 18 weeks During Pregnancy 1 days During Pregnancy 48 days During Pregnancy - Pregnancy Positive - - Rat Oral: 56400 mg/kg During - Positive - Woman Female Unreported: - Positive - Rat Unreported: - Positive - Rat Unreported: No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. No known significant effects or critical hazards. 12 . Ecological information Environmental effects United States Aquatic ecotoxicity Conclusion/Summary Other adverse effects : No known significant effects or critical hazards. : Not available. : No known significant effects or critical hazards. Waste disposal : The generation of waste should be avoided or minimized wherever possible. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Hazardous waste : Within the present knowledge of the supplier, this product is not regarded as hazardous waste, as defined by EU Directive 91/689/EEC. Pregnancy - : : : : : : 13 . Disposal considerations Pregnancy Subcutaneous: Chronic effects Carcinogenicity Mutagenicity Teratogenicity Developmental effects Fertility effects - Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. 14 . Transport information Regulatory information UN number Proper shipping name Classes PG* DOT Classification Not regulated. - - - IATA-DGR Class Not available. Not available. Not available. - Pregnancy Conclusion/Summary 27 mg/kg 500 mg/kg 50 mg/kg 15 weeks During Pregnancy 4 days During Pregnancy 6 days During Pregnancy : Not available. 7/10 7/26/2010. Biosciences Division Pierce PG* : Packing group 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce 8/10 7/26/2010. Biosciences Division Pierce 3747 N. Meridian Rd. PO Box 117 Rockford, IL 61105 (815) 968-0747 (815) 968-7316 Fax www.thermo.com/pierce Wash Buffer, 10X Dulbecco's PBS Wash Buffer, 10X Dulbecco's PBS 15 . Regulatory information 16 . Other information United States HCS Classification The customer is responsible for determining the PPE code for this material. : National Fire Protection Association (U.S.A.) U.S. Federal regulations : Irritating material Target organ effects : United States inventory (TSCA 8b): All components are listed or exempted. SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: sodium chloride; sodium phosphate, dibasic SARA 311/312 MSDS distribution - chemical inventory - hazard identification: sodium chloride: Immediate (acute) health hazard; sodium phosphate, dibasic: Immediate (acute) health hazard Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: sodium phosphate, dibasic Clean Air Act (CAA) 112 accidental release prevention: No products were found. Clean Air Act (CAA) 112 regulated flammable substances: No products were found. Clean Air Act (CAA) 112 regulated toxic substances: No products were found. Canada WHMIS (Canada) Canadian lists Canada inventory EU regulations : Class D-2B: Material causing other toxic effects (Toxic). : CEPA Toxic substances: None of the components are listed. Canadian ARET: None of the components are listed. Canadian NPRI: None of the components are listed. Alberta Designated Substances: None of the components are listed. Ontario Designated Substances: None of the components are listed. Quebec Designated Substances: None of the components are listed. International regulations International lists Health 2 0 Instability Special Date of printing : 7/26/2010. : 7/26/2010. Date of issue Date of previous issue : 6/9/2008. Version : 1.04 Indicates information that has changed from previously issued version. Full text of R-phrases : R36/37/38- Irritating to eyes, respiratory system and skin. referred to in sections 2 and 3 - Europe : Xi - Irritant Full text of classifications referred to in sections 2 and 3 - Europe Notice to reader To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. : Canada inventory: All components are listed or exempted. : This product is not classified according to EU legislation. : Risk phrases Flammability 0 This product has been classified according to the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. : Australia inventory (AICS): All components are listed or exempted. China inventory (IECSC): All components are listed or exempted. Korea inventory (KECI): All components are listed or exempted. Philippines inventory (PICCS): All components are listed or exempted. Japan inventory (ENCS): All components are listed or exempted. 16 . Other information Label requirements Hazardous Material Information System (U.S.A.) : CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. 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