MSDS# 7898 COVER SHEET 8404902 Whole Cell Stain Toolbox Kit

MSDS# 7898 COVER SHEET
8404902
Whole Cell Stain Toolbox Kit
Component #
8403202
1860101
1860288
1860289
1860290
1860291
Description
Cellomics Whole Cell Stain
Hoechst Dye
Wash Buffer 10X
Wash Buffer II
Permeabilization Buffer 10X
Blocking Buffer 10X
Life Science Research
PO Box 117
Rockford, IL
(815) 968-0747
Pierce Biotechnology Inc.
3747 N. Meridian Road
61105
(815) 968-7316 fax
www.thermo.com
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Part of Thermo Fisher Scientific
Wash Buffer, 10X Dulbecco's PBS
2.
Material Safety Data Sheet
1.
Product and company identification
: Wash Buffer, 10X Dulbecco's PBS
: Env Sensors Subassembly; Wash Buffer 10X Dulbecco's PBS
Manufacturer
: Thermo Fisher Scientific
: Thermo Fisher Scientific
Pierce Biotechnology
Pierce Biotechnology
P.O. Box 117
P.O. Box 117
Rockford, IL 61105
Rockford, IL 61105
United States
United States
815.968.0747 or
815.968.0747
800.874.3723
800.874.3723
Product name
Synonym
Supplier
Product No.
MSDS #
Validation date
Print date
Responsible name
In case of emergency
2.
:
:
:
:
:
:
1860288 1862330 1901715 8408200
7512
7/26/2010.
7/26/2010.
MSDS Specialist
CHEMTREC: Use of
800.424.9300 Substance/Preparation
OUTSIDE US:
202.483.7616
Emergency overview
Ingestion
Skin
Eyes
Refer to the instruction booklet for
proper and intended use.
Otherwise, contact supplier for
specific applications.
: Liquid.
: Odorless.
: This material is considered hazardous by the OSHA Hazard Communication Standard
(29 CFR 1910.1200).
: WARNING !
CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS
MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE.
Irritating to eyes, respiratory system and skin. Avoid exposure - obtain special
instructions before use. Do not breathe vapor or mist. Avoid contact with eyes, skin and
clothing. Contains material that can cause target organ damage. Use only with
adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash
thoroughly after handling.
(815) 968-0747
(815) 968-7316 Fax
: Pre-existing disorders involving any target organs mentioned in this MSDS as being at
risk may be aggravated by over-exposure to this product.
The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments.
Classification
: Not classified.
See toxicological information (section 11)
3.
Composition/information on ingredients
United States
Name
Sodium Chloride
Sodium Phosphate, Dibasic
Europe
Substance/preparation
Ingredient name
CAS number
7647-14-5
7558-79-4
%
7 - 10
1-3
: Preparation
CAS
number
7558-79-4
%
EC number
1-3
231-448-7
Classification
[1]
Xi;
R36/37/38
There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and
in the concentrations applicable, are classified as hazardous to health or the environment and hence require
reporting in this section.
[1] Substance classified with a health or environmental hazard
[2] Substance with a workplace exposure limit
Occupational exposure limits, if available, are listed in section 8.
1/10
Rockford, IL
61105
Medical conditions
aggravated by overexposure
Sodium Phosphate, Dibasic
7/26/2010.
3747 N. Meridian Rd.
PO Box 117
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
Contains material which causes damage to the following organs: mucous membranes,
skin.
Contains material which may cause damage to the following organs: eyes, stomach.
: Adverse symptoms may include the following:
respiratory tract irritation
coughing
: No specific data.
: Adverse symptoms may include the following:
irritation
redness
: Adverse symptoms may include the following:
pain or irritation
watering
redness
Inhalation
: Eye contact. Inhalation. Ingestion.
Routes of entry
Potential acute health effects
Inhalation
: Irritating to respiratory system.
: No known significant effects or critical hazards.
Ingestion
: Irritating to skin.
Skin
: Irritating to eyes.
Eyes
Potential chronic health effects
Chronic effects
: Contains material that can cause target organ damage.
Carcinogenicity
: No known significant effects or critical hazards.
Biosciences Division
Pierce
:
:
:
:
:
Over-exposure signs/symptoms
Hazards identification
Physical state
Odor
OSHA/HCS status
Hazards identification
Mutagenicity
Teratogenicity
Developmental effects
Fertility effects
Target organs
www.thermo.com/pierce
2/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
Wash Buffer, 10X Dulbecco's PBS
Wash Buffer, 10X Dulbecco's PBS
4.
6.
First aid measures
: Move exposed person to fresh air. If it is suspected that fumes are still present, the
rescuer should wear an appropriate mask or self-contained breathing apparatus. Keep
person warm and at rest. If not breathing, if breathing is irregular or if respiratory arrest
occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous
to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention.
If unconscious, place in recovery position and get medical attention immediately.
Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband.
Inhalation
: Wash out mouth with water. Remove dentures if any. Move exposed person to fresh
air. Keep person warm and at rest. If material has been swallowed and the exposed
person is conscious, give small quantities of water to drink. Stop if the exposed person
feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do
so by medical personnel. If vomiting occurs, the head should be kept low so that vomit
does not enter the lungs. Get medical attention. Never give anything by mouth to an
unconscious person. If unconscious, place in recovery position and get medical
attention immediately. Maintain an open airway. Loosen tight clothing such as a collar,
tie, belt or waistband.
Ingestion
Skin contact
Eye contact
Protection of first-aiders
: Flush contaminated skin with plenty of water. Remove contaminated clothing and
shoes. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing
before reuse. Clean shoes thoroughly before reuse.
: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting
the upper and lower eyelids. Check for and remove any contact lenses. Get medical
attention.
: No action shall be taken involving any personal risk or without suitable training. If it is
suspected that fumes are still present, the rescuer should wear an appropriate mask or
self-contained breathing apparatus. It may be dangerous to the person providing aid to
give mouth-to-mouth resuscitation.
Accidental release measures
Personal precautions
: No action shall be taken involving any personal risk or without suitable training.
Evacuate surrounding areas. Keep unnecessary and unprotected personnel from
entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist.
Provide adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Put on appropriate personal protective equipment (see section 8).
Environmental precautions
: Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains
and sewers. Inform the relevant authorities if the product has caused environmental
pollution (sewers, waterways, soil or air).
: Stop leak if without risk. Move containers from spill area. Approach release from
upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash
spillages into an effluent treatment plant or proceed as follows. Contain and collect
spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or
diatomaceous earth and place in container for disposal according to local regulations
(see section 13). Dispose of via a licensed waste disposal contractor. Contaminated
absorbent material may pose the same hazard as the spilled product. Note: see section
1 for emergency contact information and section 13 for waste disposal.
Large spill
: Stop leak if without risk. Move containers from spill area. Dilute with water and mop up
if water-soluble or absorb with an inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste disposal contractor.
Small spill
7.
Handling and storage
Handling
: Put on appropriate personal protective equipment (see section 8). Eating, drinking and
smoking should be prohibited in areas where this material is handled, stored and
processed. Workers should wash hands and face before eating, drinking and smoking.
Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest.
Use only with adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Keep in the original container or an approved alternative made from a
compatible material, kept tightly closed when not in use. Empty containers retain
product residue and can be hazardous. Do not reuse container.
: In a fire or if heated, a pressure increase will occur and the container may burst.
Storage
: Use an extinguishing agent suitable for the surrounding fire.
: None known.
: Promptly isolate the scene by removing all persons from the vicinity of the incident if
there is a fire. No action shall be taken involving any personal risk or without suitable
training.
: Decomposition products may include the following materials:
phosphorus oxides
halogenated compounds
metal oxide/oxides
: Store in accordance with local regulations. Store in original container protected from
direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials
(see section 10) and food and drink. Keep container tightly closed and sealed until ready
for use. Containers that have been opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled containers. Use appropriate containment
to avoid environmental contamination.
8.
See section 11 for more detailed information on health effects and symptoms.
5.
Fire-fighting measures
Flammability of the product
Extinguishing media
Suitable
Not suitable
Special exposure hazards
Hazardous combustion
products
Special protective
equipment for fire-fighters
: Fire-fighters should wear appropriate protective equipment and self-contained breathing
apparatus (SCBA) with a full face-piece operated in positive pressure mode.
3/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
Exposure controls/personal protection
Europe
No exposure limit value known.
Consult local authorities for acceptable exposure limits.
Recommended monitoring
: If this product contains ingredients with exposure limits, personal, workplace atmosphere
or biological monitoring may be required to determine the effectiveness of the ventilation
procedures
or other control measures and/or the necessity to use respiratory protective equipment.
Engineering measures
: Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor
or mist, use process enclosures, local exhaust ventilation or other engineering controls
to keep worker exposure to airborne contaminants below any recommended or statutory
limits.
4/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
Wash Buffer, 10X Dulbecco's PBS
Wash Buffer, 10X Dulbecco's PBS
8.
11 . Toxicological information
Exposure controls/personal protection
Hygiene measures
Personal protection
Respiratory
Hands
Eyes
Skin
Environmental exposure
controls
9.
: Wash hands, forearms and face thoroughly after handling chemical products, before
eating, smoking and using the lavatory and at the end of the working period. Appropriate
techniques should be used to remove potentially contaminated clothing. Wash
contaminated clothing before reusing. Ensure that eyewash stations and safety showers
are close to the workstation location.
: Use a properly fitted, air-purifying or air-fed respirator complying with an approved
standard if a risk assessment indicates this is necessary. Respirator selection must be
based on known or anticipated exposure levels, the hazards of the product and the safe
working limits of the selected respirator.
: Chemical-resistant, impervious gloves complying with an approved standard should be
worn at all times when handling chemical products if a risk assessment indicates this is
necessary.
: Safety eyewear complying with an approved standard should be used when a risk
assessment indicates this is necessary to avoid exposure to liquid splashes, mists,
gases or dusts.
: Personal protective equipment for the body should be selected based on the task being
performed and the risks involved and should be approved by a specialist before handling
this product.
: Emissions from ventilation or work process equipment should be checked to ensure they
comply with the requirements of environmental protection legislation. In some cases,
fume scrubbers, filters or engineering modifications to the process equipment will be
necessary to reduce emissions to acceptable levels.
Physical and chemical properties
:
:
:
:
:
Physical state
Color
Odor
pH
Solubility
United States
Acute toxicity
Product/ingredient name
sodium phosphate, dibasic
Liquid.
Colorless.
Odorless.
6.6 to 7
Easily soluble in the following materials: cold water and hot water.
sodium chloride
Conclusion/Summary
Chronic toxicity
Conclusion/Summary
Carcinogenicity
Conclusion/Summary
Classification
Product/ingredient name
sodium chloride
sodium phosphate, dibasic
Mutagenicity
Product/ingredient name
sodium chloride
10 . Stability and reactivity
Chemical stability
Conditions to avoid
Incompatible materials
Hazardous decomposition
products
Possibility of hazardous
reactions
Result
LD50 Oral
LDLo
Intraperitoneal
LDLo
Intramuscular
LDLo
Subcutaneous
LD50 Dermal
LD50
Intraperitoneal
LD50 Oral
LDLo
Intraperitoneal
LDLo
Subcutaneous
LC50 Inhalation
Dusts and mists
: Not available.
Species
Rat
Rat
Dose
17000 mg/kg
1 g/kg
Exposure
-
Rat
1 g/kg
-
Rat
1 g/kg
-
Rabbit
Rat
10000 mg/kg
2600 mg/kg
-
Rat
Rat
3000 mg/kg
3.72 g/kg
-
Rat
3500 mg/kg
-
Rat
>42 gm/m3
1 hours
: Not available.
: Not available.
ACGIH
-
IARC
-
Test
DNA Inhibition
Unscheduled DNA
Synthesis (UDS)
Other Mutation Test
Systems
Cytogenetic Analysis
DNA Damage
: The product is stable. Under normal conditions of storage and use, hazardous
polymerization will not occur.
: No specific data.
: No specific data.
: Under normal conditions of storage and use, hazardous decomposition products should
not be produced.
-
: Will not occur.
Micronucleus Test
DNA Damage
Cytogenetic Analysis
Cytogenetic Analysis
5/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
EPA
-
NIOSH
None.
None.
NTP
-
Experiment
Mammalian-Human;
Somatic
Mammalian-Animal
Result
Positive
Mammalian-Animal
Positive
Mammalian-Animal
Mammalian-Animal;
Somatic
Mammalian-Animal;
Somatic
Mammalian-Animal;
Somatic
Mammalian-Animal;
Somatic
Mammalian-Animal;
Somatic
Mammalian-Animal;
Somatic
Positive
Positive
OSHA
None.
None.
Positive
Positive
Positive
Positive
Positive
Positive
6/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
Wash Buffer, 10X Dulbecco's PBS
Wash Buffer, 10X Dulbecco's PBS
11 . Toxicological information
11 . Toxicological information
Conclusion/Summary
Teratogenicity
Product/ingredient name
sodium chloride
Conclusion/Summary
Reproductive toxicity
Product/ingredient name
sodium chloride
: Not available.
Europe
Result
Positive Intraperitoneal
Positive Subcutaneous
Positive Subcutaneous
Positive Subcutaneous
Not
available.
:
Maternal
toxicity
-
-
Fertility
Species
Rat
Dose
1710 mg/kg
Mouse
2500 mg/kg
Mouse
1900 mg/kg
Mouse
1900 mg/kg
Exposure
13 days During
Pregnancy
10 days During
Pregnancy
10 days During
Pregnancy
11 days During
Pregnancy
Species
Dose
-
Development
toxin
-
Rat
Intraperitoneal:
Positive
-
Mouse
10 g/kg
During
Exposure
-
13440
mg/kg
During
Positive
-
Positive
-
-
Positive
-
-
-
-
-
Mammal - Unreported:
6 g/kg
species
unspecified
Parenteral:
Rat
10
mg/kg
Mammal - Unreported:
480
species
unspecified mg/kg
Rat
Oral:
145
g/kg
During
-
18 weeks
During
Pregnancy
1 days
During
Pregnancy
48 days
During
Pregnancy
-
Pregnancy
Positive
-
-
Rat
Oral:
56400
mg/kg
During
-
Positive
-
Woman Female
Unreported:
-
Positive
-
Rat
Unreported:
-
Positive
-
Rat
Unreported:
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
No known significant effects or critical hazards.
12 . Ecological information
Environmental effects
United States
Aquatic ecotoxicity
Conclusion/Summary
Other adverse effects
: No known significant effects or critical hazards.
: Not available.
: No known significant effects or critical hazards.
Waste disposal
: The generation of waste should be avoided or minimized wherever possible. Dispose of
surplus and non-recyclable products via a licensed waste disposal contractor. Disposal
of this product, solutions and any by-products should at all times comply with the
requirements of environmental protection and waste disposal legislation and any regional
local authority requirements. Avoid dispersal of spilled material and runoff and contact
with soil, waterways, drains and sewers.
Hazardous waste
: Within the present knowledge of the supplier, this product is not regarded as
hazardous waste, as defined by EU Directive 91/689/EEC.
Pregnancy
-
:
:
:
:
:
:
13 . Disposal considerations
Pregnancy
Subcutaneous:
Chronic effects
Carcinogenicity
Mutagenicity
Teratogenicity
Developmental effects
Fertility effects
-
Disposal should be in accordance with applicable regional, national and local laws and regulations.
Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
for additional handling information and protection of employees.
14 . Transport information
Regulatory
information
UN number
Proper shipping
name
Classes
PG*
DOT Classification
Not
regulated.
-
-
-
IATA-DGR Class
Not available. Not available.
Not available.
-
Pregnancy
Conclusion/Summary
27
mg/kg
500
mg/kg
50
mg/kg
15 weeks
During
Pregnancy
4 days
During
Pregnancy
6 days
During
Pregnancy
: Not available.
7/10
7/26/2010.
Biosciences Division
Pierce
PG* : Packing group
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
8/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
Wash Buffer, 10X Dulbecco's PBS
Wash Buffer, 10X Dulbecco's PBS
15 . Regulatory information
16 . Other information
United States
HCS Classification
The customer is responsible for determining the PPE code for this material.
:
National Fire Protection
Association (U.S.A.)
U.S. Federal regulations
: Irritating material
Target organ effects
: United States inventory (TSCA 8b): All components are listed or exempted.
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: sodium chloride; sodium phosphate,
dibasic
SARA 311/312 MSDS distribution - chemical inventory - hazard identification:
sodium chloride: Immediate (acute) health hazard; sodium phosphate, dibasic:
Immediate (acute) health hazard
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: sodium phosphate, dibasic
Clean Air Act (CAA) 112 accidental release prevention: No products were found.
Clean Air Act (CAA) 112 regulated flammable substances: No products were found.
Clean Air Act (CAA) 112 regulated toxic substances: No products were found.
Canada
WHMIS (Canada)
Canadian lists
Canada inventory
EU regulations
: Class D-2B: Material causing other toxic effects (Toxic).
: CEPA Toxic substances: None of the components are listed.
Canadian ARET: None of the components are listed.
Canadian NPRI: None of the components are listed.
Alberta Designated Substances: None of the components are listed.
Ontario Designated Substances: None of the components are listed.
Quebec Designated Substances: None of the components are listed.
International regulations
International lists
Health
2
0
Instability
Special
Date of printing
: 7/26/2010.
: 7/26/2010.
Date of issue
Date of previous issue
: 6/9/2008.
Version
: 1.04
Indicates information that has changed from previously issued version.
Full text of R-phrases
: R36/37/38- Irritating to eyes, respiratory system and skin.
referred to in sections 2 and
3 - Europe
: Xi - Irritant
Full text of classifications
referred to in sections 2 and
3 - Europe
Notice to reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above-named
supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the
information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present
unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot
guarantee that these are the only hazards that exist.
: Canada inventory: All components are listed or exempted.
: This product is not classified according to EU legislation.
:
Risk phrases
Flammability
0
This product has been classified according to the hazard criteria of the CPR and the MSDS contains all the information required by the CPR.
: Australia inventory (AICS): All components are listed or exempted.
China inventory (IECSC): All components are listed or exempted.
Korea inventory (KECI): All components are listed or exempted.
Philippines inventory (PICCS): All components are listed or exempted.
Japan inventory (ENCS): All components are listed or exempted.
16 . Other information
Label requirements
Hazardous Material
Information System (U.S.A.)
: CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS
MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE.
:
Health
2
Flammability
0
Physical hazards
0
9/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
10/10
7/26/2010.
Biosciences Division
Pierce
3747 N. Meridian Rd.
PO Box 117
Rockford, IL
61105
(815) 968-0747
(815) 968-7316 Fax
www.thermo.com/pierce
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