20100412b.pdf IR-4 Ornamental Horticulture Program Research Report Cover Sheet Trial: 2009 OR Researcher(s) Nik Grunwald Affiliation: USDA-ARS Horticultural Crops Research 3420 NW Orchard Ave. Corvalis OR 97330 PhoneNumber: 541-738-4049 [email protected] Email: ProjectTitle: Protocol #: Phytophthora Efficacy 09-003 PR# Research Target Crop/Plant Product EPA Reg. # Production Site 29718 Phytophthora ramorum Arrowwood Adorn 4F 59638-140 Phytophthora ramorum Viburnum sp. V-10161 Valent Phytophthora ramorum Arrowwood Aliette WDG 432-890 Phytophthora ramorum Viburnum sp. Fosetyl Al Bayer Phytophthora ramorum Arrowwood BAS 651F - Phytophthora ramorum Viburnum sp. BAS 651 BASF 29722 Phytophthora ramorum Phytophthora ramorum Arrowwood Viburnum sp. Captan Captan 29719 Phytophthora ramorum Arrowwood Phytophthora ramorum Viburnum sp. 29716 Phytophthora ramorum Phytophthora ramorum 29715 Status Greenhouse C Greenhouse C Greenhouse C 51036-166 MicroFlo Greenhouse C CG100 - Greenhouse C CG100 (organic acid) Summerdale, I Arrowwood Viburnum sp. Disarm 480SC Fluoxastrobin 66330-64 OHP Greenhouse C Phytophthora ramorum Arrowwood NOA 446510 - Greenhouse C Phytophthora ramorum Viburnum sp. Mandipropamide Syngenta Phytophthora ramorum Arrowwood Regalia 5O (MOI-10605) - Greenhouse C Phytophthora ramorum Viburnum sp. Extract of Reynoutria sachali Marrone Organ 29724 Phytophthora ramorum Phytophthora ramorum Arrowwood Viburnum sp. Stature DM Dimethomorph 241-419-67690 Greenhouse SePro C 29723 Phytophthora ramorum Arrowwood Subdue MAXX 100-796 C Phytophthora ramorum Viburnum sp. Mefenoxam Syngenta 29721 29717 29720 Greenhouse VIBURNUM (Viburnum plicatum ‘Mariesii’) Ramorum blight; Phytophthora ramorum N.J. Grunwald and K. Rolfe USDA-ARS Horticultural Crops Research Lab 3420 NW Orchard Ave., Corvallis, OR 97330 Efficacy of registered and unregistered fungicides for control of Ramorum blight on Viburnum, 2009. This study was conducted as part of the IR-4 Ornamental Horticulture Program to evaluate fungicides for managing foliar diseases of ornamental plants caused by Phytophthora ramorum. Because P. ramorum is a pathogen under federal quarantine, experiments had to be conducted in containment on detached leaves. Viburnum plants grown in 3.8-L plastic pots were obtained in May and maintained outdoors in shade conditions with daily hand watering. Four single-plant replications were arranged in a randomized block design. Treatments consisted of a fungicide application on 21 Aug or 25 Aug of either a spray or drench. Foliar applications were applied to run-off using a hand-pump sprayer. Drenches were applied until soil was saturated. Either seven or three days depending on product after fungicide application, three leaves from each plant were removed for detached leaf inoculations under quarantine containment conditions. Leaves were placed in plastic containers with 500 ml moistened vermiculite. Separate containers were used for each chemical treatment. Each leaf was needlewounded once on the abaxial side to the right of the midvein just prior to inoculation with P. ramorum strain 0374-N10A (A2 mating type isolated in OR). A 20-µl drop of inoculum (3,500 sporangia/ml) was applied to each wound site. Non-inoculated controls received an equivalent drop of sterile distilled water. After inoculation, leaves were misted and containers were placed in an incubator at 20ºC with a 14-hour light cycle for ten days. Disease severity was determined by evaluating lesion area from digital images using ASSESS software (APS, St. Paul, MN) on day seven. Lesion area was subjected to analysis of variance (JMP 8, SAS Institute, Cary, NC). All experiments were conducted in a containment growth chamber following standard operating procedures approved by USDA APHIS. Chemical treatments had a significant effect on lesion area observed. However, not all chemicals had the same efficacy in reducing lesion expansion. Aliette, Captan, Stature DM, NOA 446510, BASF 651F (low rate only), and V-10161 were not significantly different from the untreated, inoculated controls. Disarm, BASF 651F( high rate), CG100, Regalia SC, and Subdue MAXX were most effective in reducing disease severity. All chemicals were effectively fungistatic, not fungicidal, after confirmation of the pathogen's viability by isolation and subsequent culture of the pathogen from wound sites on symptomless leaves. No phytotoxicity was observed for any treatment. The non-inoculated control showed no lesions. Treatment and rate/L Application Lesion area (cm2)* NOA 446510 (mandipropamid) 0.31 ml ......................................... spray 2.66 BCDE 2.36 CDE NOA 446510 (mandipropamid) 0.62 ml ......................................... spray 2.16 DEF Disarm 480SC (fluoxastrobin) 0.31 ml ............................................ drench 1.34 FG Disarm 480SC (fluoxastrobin) 0.62 ml ........................................... drench 2.79 BCDE BASF 651F 1.71 ml ......................................................................... spray 1.93 EF BASF 651F 2.80 ml ......................................................................... spray 3.87 A V-10161 (fluopicolide) 0.08 ml ....................................................... drench 3.46 AB V-10161 (fluopicolide) 0.16 ml ....................................................... drench 0.83 GH Cg100 0.3% ..................................................................................... spray 0.84 GH Cg100 0.6% ..................................................................................... spray 0.42 GH Regalia SC 0.5% .............................................................................. spray 0.16 H Regalia SC 1% ................................................................................. spray 2.76 BCDE Aliette (fosetyl Al) 0.71 g ................................................................ drench 3.05 ABCD Captan 2.4 g ...................................................................................... spray 0.05 H Subdue MAXX (mefenoxam) 0.16 ml ............................................ spray 2.19 CDEF Stature DM (dimethomorph) 0.48 ml .............................................. spray 3.13 ABC Untreated, inoculated control .......................................................... * Means followed by the same letter are not significantly different at P=0.05, according to a Fisher's protected least significance difference test. Project Number: 09-003 Viburnum plicatum tomentosum 'Mariesii' PHYTOTOXICITY DATA Report Dates 8/21/2009 8/25/2009 8/29/2009 9/2/2009 Treatments Trial #1 Adorn lo 24907 Adorn hi 24907 Bas 651 lo 28771 Bas 651 hi 28771 cg100 lo 29120 cg100 hi 29120 Disarm lo 25724 Disarm hi 25724 Regalia lo 29121 Regalia hi 29121 NOA lo 24905 NOA hi 24905 Aliette Captan Subdue Stature Controls 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Index of Injury ( 0 = No Injury, 10 = Complete Kill) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9/5/2009 9/9/2009 9/19/2009 9/23/2009 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Project Number: PRNumber: 09-003 24907 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: T d Name: Trade N Manufacturer: Target Pests: Lot number: EPA number: Fluopicolide Ad Adorn 4SC Valent Foliar pathogens V08D-15Sc-2 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Drench 1 Growth Stage: Vegetative Measured amount 1floz/100gal 2floz/100gal 8/21/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 28771 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: T d Name: Trade N Manufacturer: Target Pests: Batch Number: EPA Number: BAS651F BAS651F BASF Foliar pathogens APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Trial #2 Growth Stage: Foliar 1 Measured amount 22floz/100gal 32floz/100gal 8/21/2009 Vegetative Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 29120 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: Lot Number: Cg100 Cg100 g Summerdale Foliar pathogens APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Foliar 1 Variable Cone 30 psi Measured amount .4% .8% Growth Stage: Vegetative 8/25/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 25724 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Lot Number: Fluoxastrobin Disarm 480SC OHP Foliar pathogens 0D053006001 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Drench 1 Growth Stage: Vegetative Measured amount 4floz/100gal 8floz/100gal 8/21/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 24905 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Mandipropamide NOA 446510 Syngenta Foliar pathogens 60220115 120116-88-3 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Foliar 1 Variable Cone 30 psi Measured amount 4oz/100gal Lo 8oz/100gal Hi Growth Stage: Vegetative 8/25/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 29121 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical C e ca Name: a e Trade Name: Manufacturer: Target Pests: Lot number: EPA number: Fluoxastrobin uo ast ob Regalia SC Arysta Foliar pathogens 0D053006001 66330-64 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Foliar 1 Variable Cone 30 psi Measured amount .5% 1% Growth Stage: Vegetative 8/25/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: 09-003 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Fosetyl Al Aliette Bayer Foliar pathogens 264-467 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Trial #2 Growth Stage: drench 1 Measured amount 9.4oz/100gal 8/21/2009 Vegetative Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: 09-003 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Trichloromethylthio cyclohexene Captan Arysta Foliar pathogens 303-516 0090131-A APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Foliar 1 Variable Cone 30 psi Measured amount 32oz/100gal Growth Stage: Vegetative 8/25/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: 09-003 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Common Name: Trial Site: First Transplant: Final Pot Size: Plot Spacing: Experiment Design: Number of Reps: Soil Type: Soil Components: Fertilizer: Irrigation: Viburnum plicatum tomentosum 'Mariesii' Doublefile viburnum Field 5/15/2009 1 gallon 0" x 0" Random Block 4 Mushroom compost, sand, soil and fir bark Clay 35%, Silt 10%, sand 10%, OM 45% Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Mefonaxam Subdue Maxx Syngenta Foliar pathogens 100-796 APPLICATION Application Type: Application Number: Nozzle Type: Nozzle Pressure: Calibration Date: Rate Applied: Application Dates: Trial #1 Foliar 1 Variable Cone 30 psi Measured amount 2oz/100gal Growth Stage: Vegetative 8/21/2009 Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. Project Number: PRNumber: 09-003 26803 INVESTIGATOR: Name: Address: Phone Number: Trial Location: Dr. Niklaus J. Grunwald 3420 NW Orchard Street Corvallis, OR. 97330 (541) 738-4049 27303 Peoria Road, Corvallis, OR. PLANT CULTURE Scientific Name: Viburnum plicatum tomentosum 'Mariesii' Common Name: Doublefile viburnum Trial Site: Field First Transplant: 5/15/2009 Final Pot Size: 1 gallon Plot Spacing: 0" x 0" Experiment Design Random Block Number of Reps: 4 Soil Type: Mushroom compost, sand, soil and fir bark Soil Components: Clay 35%, Silt 10%, sand 10%, OM 45% Fertilizer: Osmocote Plus 1/2 tsp applied at transplant 20-20-20 fertilizer 1/16 ratio applied on weekly basis Irrigation: Hand-watered as needed PESTICIDE Chemical Name: Trade Name: Manufacturer: Target Pests: Batch Number: EPA Number: Dimethomorph Stature BASF Foliar pathogens 474-275 APPLICATION Application Type: Foliar Application Numbe 1 Nozzle Type: Variable Cone Nozzle Pressure: 30 psi Calibration Date: Measured amount Rate Applied: 6.4oz/100gal Application Dates: Trial #1 8/21/2009 Growth Stage: Vegetative Narrative Summary of Methods Plants were obtained from a nursery in 4" liners. They were transplanted into 1 gallon pots and hand-watered as needed. The plants were grouped into random blocks of 8 plants each and the chemical treatments were applied. Observations for phytotoxicity were performed 7, 14 and 28 days after each of the 2 applications. No phytotoxicity was observed on any plants at any of the observation dates. The plants were 8" to 10" in height and they were well formed and saleable. WEATHER CONDITIONS Hydromet Archive Data USBR Pacific Northwest Region, Corvallis, Oregon DATE 5/15/2009 5/16/2009 5/17/2009 5/18/2009 5/19/2009 5/20/2009 5/21/2009 5/22/2009 5/23/2009 5/24/2009 5/25/2009 5/26/2009 5/27/2009 5/28/2009 5/29/2009 5/30/2009 5/31/2009 6/1/2009 6/2/2009 6/3/2009 6/4/2009 6/5/2009 6/6/2009 6/7/2009 6/8/2009 6/9/2009 6/10/2009 6/11/2009 6/12/2009 6/13/2009 6/14/2009 6/15/2009 6/16/2009 6/17/2009 6/18/2009 6/19/2009 6/20/2009 6/21/2009 6/22/2009 6/23/2009 6/24/2009 6/25/2009 6/26/2009 6/27/2009 6/28/2009 6/29/2009 6/30/2009 7/1/2009 7/2/2009 7/3/2009 7/4/2009 7/5/2009 7/6/2009 7/7/2009 7/8/2009 7/9/2009 7/10/2009 7/11/2009 7/12/2009 7/13/2009 7/14/2009 7/15/2009 7/16/2009 7/17/2009 7/18/2009 7/19/2009 7/20/2009 7/21/2009 7/22/2009 7/23/2009 7/24/2009 7/25/2009 7/26/2009 7/27/2009 7/28/2009 7/29/2009 7/30/2009 7/31/2009 MM 53.93 59.81 62.63 59.32 51.6 50.25 56.11 55.82 55.2 54.65 55.59 58.11 61.03 65.91 67.69 68.56 62.79 63.09 65.29 68.22 62.72 56.97 57.73 58.06 59.54 56.5 59.7 61.33 62.79 61.79 60.95 64.42 63.01 62.47 63 61.21 56.7 57.19 56.32 63.51 61.86 60.41 60.3 64.09 63.1 61.78 62.99 68.39 72.76 70.39 69.82 61.8 60.44 57.7 60.23 62.83 67.42 61.12 58.78 60.44 63.04 69.71 74.04 73.62 69.86 67.22 71.84 67.8 64.02 63.54 68.01 72.34 73.38 82.76 87.63 83.49 71.39 70.66 MN 41.58 40.23 44.53 46.45 41.28 35.89 41.5 40.62 40.38 38.1 37.33 42.09 47.38 49.15 50.07 52.73 46.21 51.74 55.83 58.69 55.84 52.33 53.96 53.32 51.52 46.53 53.18 55.75 52.65 56.77 54.89 56.35 50.75 51.86 52.68 54.37 51.26 47.21 40.86 48.73 46.77 55.03 48.67 47.1 49.1 46.35 46.79 48.32 54.55 49.36 47.22 47.75 51.61 45.95 52.65 51.24 55.67 52.71 56.55 52.76 49.87 53.63 55.56 55.8 53.04 51.69 55.8 51.66 50.18 50.77 52.89 53.67 54.97 65.56 72.4 64.41 58.71 53.39 MX 67.47 79.9 79.7 76.9 57.87 61.83 70.2 71.1 71.2 70.6 72.9 73.9 75.8 82.5 84 85.1 80.5 76.2 77.6 83 75.7 62.13 63.22 64.2 69.46 65.28 66.74 69.01 74.6 71.2 69.77 75.7 75 70.6 74.3 68.41 65.28 66.68 68.01 78.7 77.9 68.46 71.4 82.1 76.9 77.4 78.2 86.8 91.1 90.1 91.8 76.1 69.06 69.38 69.99 74.3 82.9 70.7 64.38 70.1 77.3 87.3 90.8 91.8 87 82.2 89.5 86 80.6 78.3 85.2 91.5 91.7 100.2 103.9 102.5 88.2 91.9 PC 24.36 24.36 24.36 24.36 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.49 24.52 24.52 24.63 24.74 24.92 24.92 24.93 24.93 24.93 24.93 24.93 24.93 25.06 25.06 25.06 25.06 25.06 25.06 25.07 25.07 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.08 25.75 25.81 25.81 25.81 25.81 25.81 25.81 25.81 25.81 25.81 25.81 25.81 25.82 25.82 25.82 25.82 25.82 25.82 25.82 25.82 PP 0 0 0 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0 0.11 0.11 0.18 0 0.01 0 0 0 0 0 0.13 0 0 0 0 0 0.01 0 0.01 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.67 0.06 0 0 0 0 0 0 0 0 0 0 0.01 0 0 0 0 0 0 0 SR 707.6 711.95 722.99 718.69 540.14 719.51 730.37 717.05 720.73 726.88 708.45 645.71 719.92 747.18 702.68 717.28 720.32 564.13 400.55 602.87 301.95 295.39 356.26 403.22 637.41 474.45 346.02 351.42 611.4 453.64 526.2 658.63 473.31 457.13 575.21 671.53 426.3 546.98 704.93 728.55 742.67 415.31 768.09 765.43 766.65 771.98 771.17 774.44 756.41 756.2 757.02 698.81 518.41 544.86 410.8 578.47 651.66 406.08 162.35 420.64 721.35 732.63 734.47 674.9 710.41 738.77 719.51 706.59 606.16 571.91 676.67 674.82 677.29 669.28 675.84 670.32 646.73 667.04 TA 73.36 67.89 68.97 71.44 76.17 72.14 67.7 74.88 74.61 73.73 67.1 70.02 70.04 60.85 69.29 58.43 68.72 78.15 76.94 76.96 91.7 90.7 81.99 76.68 73.71 82.61 76.15 79.36 78.17 84.03 77.1 73.37 78.97 75.93 73.11 73.86 73.16 66.87 67.9 62.38 70.02 70.64 60.26 58.02 61.17 59.49 51.46 44.73 42.02 54.64 55.42 64.73 65.44 71.85 67.84 69.03 64.65 77.71 86.71 83.4 70.17 63.7 57.46 60.32 61.39 59.05 55.87 67.9 74.28 71.44 63.27 64.16 62.92 53.55 44.75 53.12 67.38 67.15 MN= MINIMUM 24 HOUR AIR TEMPERATURE (Midnigh MX= MAXIMUM 24 HOUR AIR TEMPERATURE (Midnigh PP= 24 HOUR TOTAL PRECIPITATION (Midnight to Mid SR= ACCUMULATED GLOBAL SOLAR RADIATION (La TA=MEAN DAILY RELATIVE HUMIDITY (%) WR=TOTAL WIND RUN (miles/day) 8/1/2009 8/2/2009 8/3/2009 8/4/2009 8/5/2009 8/6/2009 8/7/2009 8/8/2009 8/9/2009 8/10/2009 8/11/2009 8/12/2009 8/13/2009 8/14/2009 8/15/2009 8/16/2009 8/17/2009 8/18/2009 8/19/2009 8/20/2009 8/21/2009 8/22/2009 8/23/2009 8/24/2009 8/25/2009 8/26/2009 8/27/2009 8/28/2009 8/29/2009 8/30/2009 8/31/2009 9/1/2009 9/2/2009 9/3/2009 9/4/2009 9/5/2009 9/6/2009 9/7/2009 9/8/2009 9/9/2009 9/10/2009 9/11/2009 9/12/2009 9/13/2009 9/14/2009 9/15/2009 9/16/2009 9/17/2009 9/18/2009 9/19/2009 9/20/2009 9/21/2009 9/22/2009 9/23/2009 9/24/2009 9/25/2009 9/26/2009 9/27/2009 9/28/2009 9/29/2009 9/30/2009 9/30/2009 73.66 72.94 68.25 62.85 62.02 60.56 61.23 63.48 67.62 66.02 67.41 67.9 60.84 60.09 60.76 61.39 72.84 77.95 76.64 64.2 63.05 65.34 60.53 61.98 58.9 65.66 67.99 61.25 64.18 66.33 66.08 63.61 63.77 65.23 63.91 60.11 59.7 58.98 58.56 62.56 67 70.07 69.21 63.31 64.64 65.14 60.53 57.74 61.59 59.31 57.43 69.89 68.34 63.14 60.33 59.9 60.2 60.34 54.29 51.36 52.78 52.78 56.82 61.18 53.9 48.44 48.67 53.52 52.14 47.97 56.11 46.2 53.05 58.76 53.46 47.32 47.95 47.48 55.72 62.46 56.68 50.19 49.98 52.79 43.17 43.41 45.02 49.84 46.17 46.79 52.28 57.83 51 51.97 48.08 55.27 50.93 54.48 54.77 51.73 46.45 48.54 57.1 51.29 51.46 55.45 55.63 49.15 50.44 47.2 43.69 46.65 41.83 52.43 46.4 43.83 46.61 47.48 42.4 46.46 43.72 43.99 45.45 45.45 94.4 90.3 87.8 79 79.4 67.25 67.36 77.1 77.9 84.8 82 76.1 69.53 72.1 70.8 75 89.8 93.8 97.9 79.8 75.5 79.4 74.2 80.7 73.8 85.8 90.8 72.2 77.5 80.7 82 76 84.2 77.6 79.4 67.11 66.37 69.18 73.4 80 82.3 90.8 88 68.69 76.7 81.8 69.54 72.5 82.9 70.9 71 86.1 89.4 86.6 73.7 74.9 75.4 79 66.41 60.33 60.82 60.82 25.82 25.82 25.82 25.82 25.82 25.82 25.82 25.82 25.82 25.82 25.9 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 25.98 26 26 26 26.06 26.06 26.06 26.06 26.06 26.06 26.06 26.06 26.7 26.94 26.94 26.94 26.94 26.94 26.94 26.94 26.94 26.94 26.94 26.97 26.98 26.98 27.03 27.03 27.03 27.03 27.03 27.03 27.03 27.03 27.03 27.06 27.16 27.16 27.16 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.02 0 0 0.06 0 0 0 0 0 0 0 0.64 0.24 0 0 0 0 0 0 0 0 0 0.03 0.01 0 0.05 0 0 0 0 0 0 0 0 0.03 0.1 0 0 640.17 573.77 655.96 610.05 549.16 208.68 241.89 563.11 567.4 636.08 511.44 473.12 360.61 436.18 472.5 622.54 582.17 615.37 595.7 592.82 422.69 613.83 593.55 607.17 405.68 603.49 582.78 278.99 451.59 382.5 537.07 542.88 537.24 511.38 424.11 258.71 317.76 446.68 530.29 449.77 421.14 514.96 494.09 310.2 410.33 460.17 219.06 401.96 488 231.08 483.08 478.59 439.94 449.98 377.47 463 447.12 448.31 233.98 369.06 280.78 280.78 63.93 62.41 61.89 68.68 68.85 71.5 75.81 69.88 67.53 66.82 76.12 80.7 74.53 70.28 65.98 67.78 52.72 44.14 54.72 71.7 72.29 64.16 63.03 62.94 76.62 61.59 53.45 68.62 78.4 73.73 71.28 69.66 65.18 58.24 61.76 81.76 79.59 76.1 67.83 72.73 72.05 60.53 67.39 74.37 72.16 69.13 83.31 79.28 66.3 76.61 66.73 36.44 44.48 61.95 76.02 71.59 66.02 59.25 74.59 80.29 75.75 75.75 ht to Midnight) (F) ht to Midnight) (F) dnight) (inches) ngleys)
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