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Sample Preparation
81
Sample Preparation
Summary of applications
Application
Product
Page
Heating, cooling and mixing of samples in various tube and slide sizes
(13 °C below RT to 99 °C)
Thermomixer® R
108
Heating and mixing of samples in 1.5 ml microcentrifuge tubes
(4 °C above RT to 99 °C)
Eppendorf Thermomixer
106
Heating and cooling of samples in various tube and slide sizes
(–5 °C to 99 °C)
ThermoStat plus
110
Efficient mixing of samples in plates (up to 96- and 384-well formats)
and tubes (0.5 ml to 2.0 ml), additional vortex function
MixMate™
104
Device for hermetic heat sealing of 96-/384-well plates
Heat Sealer
201
Flex-Tubes®
93
Various colors
Safe-Lock microcentrifuge tubes
92
Light-sensitive samples
Safe-Lock microcentrifuge tubes, amber
92
High recovery rate for DNA and RNA,
contamination-free sample preparation
DNA LoBind tubes
91
High recovery rate for proteins,
contamination-free sample preparation
Protein LoBind tubes
90
Sterile and DNA-free sample preparation
Safe-Lock, Eppendorf Biopur
92
Eppendorf Plate™ Deepwell
96
Cell culture, sample storage, transport
Micropestle
93
Processing and storage of max. 20 samples
(1.5/2 ml tubes)
Rack 3821
102
Processing and storage of max. 24 samples
(0.5 ml to 2 ml tubes)
Tube Rack
102
Cooling (0 °C and –21 °C) and storage of max. 24 samples
(0.5 ml to 2 ml tubes)
IsoTherm system
115
Cooling (0 °C) and storage of max. 96 samples
(0.2/0.5 ml tubes and plates),
color change for temperature control
PCR-Cooler
116
Instruments
Consumables
Tubes
Single tubes
Standard application, various colors, 1.5 ml
Info | Sample Preparation
Extremely safe seal, 0.5 ml to 2 ml
82
Plates
Cell culture, sample storage, transport
Accessories
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Sample Preparation
Summary of applications
Application
Product
Page
Reusable
Sealing Mat 96 (not for 96/2,000)
203
Heat-sealing, transparent, not removable,
cannot be pierced (e.g., for colorimetric detection)
Heat Sealing Film
201
Adhesive seal, transparent, removable,
cannot be pierced
PCR Film (self-adhesive)
202
Adhesive seal, non-transparent, removable,
cannot be pierced
PCR Foil (self-adhesive)
202
Sealing options for Eppendorf Plate™ Deepwell 96 and 384
Descriptions for Eppendorf purity levels
89
Products for PCR
182
This table shows a selection of particular applications from the
Sample Preparation area and serves as an overview. The complete
product portfolio of the Eppendorf Sample Preparation System is
found on the following pages.
Sample Preparation | Info
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Look for this icon by the ordering information for
products in this section that may be custom packaged,
mixed or purity certified.
Some products may be customized for applicationspecific or OEM requirements. Further information
on page 255.
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
83
Purity levels
Eppendorf purity levels for tips, tubes, plates and Combitips®
Consumables | Sample Preparation
®
Eppendorf Quality
Eppendorf Sterile
Eppendorf PCR clean
Eppendorf Biopur
Test method
Continuous quality
control
Continuous quality
control—certified
sterilization
Batch testing1
(certified)
Batch testing1
(certified)
Quality criteria
Function,
tightness,
precision
Sterile
Free of human DNA,
DNase, RNase
and PCR inhibitors
Sterile,
free of pyrogens,
RNase, DNA and ATP
Eppendorf
product range
‡ epTIPS Bulk
‡ epTIPS Reloads
‡ epTIPS Box
‡ epTIPS Set
‡ epTIPS Racks
‡ Safe-Lock tubes
and Flex-Tubes®
‡ Combitips Plus® tips*
‡E
ppendorf Plates™
Deepwell 96 and 384
‡ epTIPS Singles
‡ epTIPS Racks
‡ Combitips Plus tips
‡E
ppendorf Plates
Deepwell 96 and 384
‡ epTIPS Reloads
‡ epTIPS Filter (PCR
clean, Sterile and
pyrogen-free)
‡ epTIPS Racks
‡ PCR Tubes
(0.2/0.5 ml)**
‡ PCR plates
‡ Capping aids
‡ Safe-Lock tubes
and Flex-Tubes
‡ Protein and DNA
LoBind tubes
‡ DNA/Protein LoBind
Eppendorf Plates
Deepwell 96 and 384
‡ epTIPS Racks
‡ epTIPS Singles
‡ Safe-Lock tubes
‡ Combitips Plus tips
Requirements
‡
‡
‡
‡
‡
‡
‡
Defined flow dynamics
Minimal water retention
High chemical resistance
High thermal stability
High centrifugal stability
Precisely molded
IVD compliance
ATP-free
DNA-free (Ph.Eur./USP)
DNase-free
Individually wrapped
Free of human DNA
Free of PCR inhibitors
Free of bacteria DNA (E. coli)
Pyrogen-free
RNase-free
Sterile
‡
‡
‡
‡
‡
‡
‡
‡
‡
‡
‡
‡
‡
‡1
‡1
‡3
‡
‡1
‡
‡1
‡
ertified; certificate enclosed
C
with product.
1
2
Certified; batch-specific
certificate can be downloaded
from www.eppendorf.com
Tips also available in boxes/racks.
3
For more details on these purity levels, see pages 85–89.
84
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Product appearance, specifications, and/or prices are subject to change without notice.
‡
‡
‡
‡
‡
‡
‡
‡2
‡2
‡
‡3
‡
‡
‡2
‡2
‡2
*U.S. Pats. 5,620,660/5,620,661
**U.S. Pat. 5,863,791
Purity levels
IVD (in vitro diagnostic products)
IvD
IVD
Continuous
quality control
Especially suitable for
diagnostic laboratories
‡ Pipette tips
‡ Deepwell plates
‡ Tubes
‡ Combitips Plus®*
‡ Pipettes
‡ Centrifuges
‡ Thermomixers®
How did we design our products to comply with IVD?
European Directive on IVD (98/79/EC).
To meet the directive, a product must meet a comprehensive list of
fundamental and standardized requirements. Analysis is conducted
What does IVD mean?
in advance to all potential IVD products, which—depending on the
IVD is the abbreviation for in vitro diagnostic tools. IVD products
type, area of use and purpose of the product—includes physical/
are generally equipment, solutions and other consumables that
chemical tests (e.g., shelf life, long-term stability, purity tests),
are used to examine samples from the human body.
performance tests and qualification of sterilization.
What advantages does this standard have for the product
For production, IVD compliance also means that QA certificates
and for you?
(ISO 9001, ISO 13485) must be available; in other words, a product
The IVD directive and associated standards (EN standards) require
has to be traceable right back to the day of production, and a
uniform quality and safety for these products throughout Europe
check on identification and preventive monitoring of the process
to ensure maximum reproducibility for your experiments and
has to be carried out.
analyses. The CE symbol, applied by the manufacturer, certifies
the product is IVD-compliant. This high degree of reliability is
In addition, instructions for proper use and the risk of incorrect
especially important when, for example, assessing physiological
operation should be enclosed with each product. All the measures
samples in a clinical lab to define therapeutic measures.
outlined guarantee that each IVD product satisfies the most
*U.S. Pats. 5,620,660/5,620,661
Sample Preparation | Consumables
IVD-compliant products meet the requirements of the new
stringent requirements for quality and purity in the laboratory.
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
85
Purity levels
Eppendorf Quality
Description
Eppendorf products with the Eppendorf Quality purity label fulfill
production, our Eppendorf® pipette tips, Eppendorf Tubes® and
everything expected of reliable, easy-to-handle consumables. With
Eppendorf Combitips Plus® tips are ideal for many lab tasks. Also
the aid of high-quality base material and permanent checks during
available: pipette tips in our newest “Sterile” purity level!
Which requirements does Eppendorf Quality fulfill?
‡ High precision and
‡ Single-handed opening
accuracy as well as defined
and closing of microcentrifuge
flow properties, ensured by
tubes, without sample material
precise molding of pipette
being contaminated
tips and Combitips Plus* tips
‡ Microcentrifuge tubes
‡ Minimal water retention,
are highly stable, even when
ensuring reliable volume
exposed to extreme thermal
readings and minimal adhesion
or mechanical strain
‡ Excellent running
‡ Good chemical stability of
properties of Combitips Plus
consumables for use in many
tips for reduced effort and
different applications
smooth liquid handling (this
Consumables | Sample Preparation
is crucial for minute volumes)
*U.S. Pats. 5,620,660/5,620,661
Quality control measures
‡ Tests for vapor tightness, lid-opening force, wall strength,
Pipette tips, microcentrifuge tubes, Deepwell plates and Combitips
force required to pierce the lid and centrifugal stability
cylinders are made of PP (polypropylene), a relatively inert
performed on microcentrifuge tubes on a daily basis
plastic characterized by minimum water retention as well as high
‡ Gravimetric measurements are performed on tips and
dimensional and mechanical stability across a wide temperature
Combitips in order to ensure international specifications
range. Other plastics, such as PE (polyethylene), are also used, for
‡ All production tools are checked throughout the entire
example in the Combitips Plus pistons; the combination of PP and
production process to ensure consistent quality
PE results in ideal running properties between these two parts.
‡ All production steps, from the completion of the goods to
packaging, are fully automated; manual intervention has been
eliminated, and all subsequent risks caused by contamination
have been virtually ruled out
A good basis: Start with top-quality material!
‡ All plastics used to
‡ Plastics are highly resistant
produce consumables
to organic and inorganic acids/
comply with international
bases and organic solvents
purity criteria
commonly used in laboratories
‡ The dyes used for
consumables are free of
organic substances and
heavy metals
86
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Product appearance, specifications, and/or prices are subject to change without notice.
Purity levels
Eppendorf PCR clean
Which requirements does “PCR clean” fulfill?
Description
With the advent of PCR (polymerase chain reaction) and many other
‡ The miniscule amounts of
‡ These plastic goods must
molecular biology methods in clinical and research labs, new purity
genetic substance used for
also be free of contaminating
criteria for consumables are necessary. Although there are no set
experiments must be protected
nucleic acids, normally human
directives in this field, we have analyzed the individual criteria and
from degradation (e.g., from
DNA, which could infiltrate
redefined our requirements governing PCR consumables.
DNases and RNases), i.e., all
the plastic goods during the
tubes and tips must be free of
production process
such impurities
(e.g., via skin particles) and
‡ Consumables that come into
thus may lead to false-positive
contact with PCR reactions
results
must be free of PCR inhibitors,
which would inter­fere with the
amplification process
Clean production, clean packaging
fully functional consumables. Production and primary packaging of
all products that are available in the PCR clean purity category are
conducted under clean room conditions. The protective outer box is
secured with a “PCR clean” seal to ensure that the product remains
uncontaminated.
Only those consumables that have passed all of the necessary
tests are given the “PCR clean” quality seal. The seal assures
you that Eppendorf® consumables are suited for direct use in
experiments and analysis without the need for further treatment
(such as autoclaving).
A batch-related PCR clean certificate is available upon request.
All production batches are tested at an independent
laboratory for the following:
Sample Preparation | Consumables
Eppendorf ensures that your lab receives clean, undamaged and
‡ Absence of human DNA: This is monitored in a PCR test
that can detect quantities smaller than a single human cell
(< 2 pg DNA)
‡ Absence of DNase and RNase: Reaction buffers with one
DNA and one RNA conductor are incubated to 37 °C in the
consumables (or, following liquid handling, in the consumables
for several hours); the intactness of the nucleic acids is then
tested via electrophoresis
‡ Absence of PCR inhibitors: The consumables are tested in
a separate PCR experiment to detect contamination by any
substances that may have an adverse effect on the amplification
procedure
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
87
Purity levels
Eppendorf Biopur
Description
All consumables in this highest-level purity category—pipette
The Eppendorf Biopur quality and purity standard meets the
tips, Combitips Plus®* and Eppendorf® microcentrifuge tubes—
requirements of the medical, pharmaceutical and food industries.
are guaranteed to be sterile and free of pyrogen, RNase, DNA and
ATP. This is achieved by a sophisticated, automated production
process with different control protocols to rule out any danger of
contamination caused by biological substances.
®
‡ Purity is ensured, even
‡ “Ready-to-use” products
for the most sophisticated
save time and labor
applications
‡ Individually wrapped
‡ Reduced costs—
consumables and
inconclusive, usually expensive
resealable boxes provide
tests are no longer necessary
effective protection against
follow-up contamination
Consumables | Sample Preparation
*U.S. Pats. 5,620,660/5,620,661
What does Eppendorf Biopur offer?
All Eppendorf Biopur products are manufactured under one
strict production process. They also meet our specific quality
requirements regarding wetting behavior, liquid tightness,
accuracy and chemical resistance, as well as the following criteria:
‡ Sterile
‡ Certified sterility in
‡ Free of pyrogen, RNases,
‡ An Eppendorf Biopur batch-
‡ Control and certification by
accordance with USP
bacteria (E. coli), human DNA
specific certificate is issued
an independent laboratory
XXII (United States
and ATP
after all test criteria are met
‡ Each individual batch is
Pharmacopoeia), DAB 10
‡ Certified free of pyrogens in
sterilized via beta irradiation
(German Pharmacopoeia)
accordance with the kinetic-
and Ph.Eur. (European
turbidimetric LAL test (FDA
Pharmacopoeia)
guidelines)
A batch-related Biopur certificate is available
at www.eppendorf.com
Individually packaged—for your safety!
End users demand that Eppendorf Biopur products arrive clean,
undamaged and fully functional. All products in the Eppendorf
Biopur purity category, such as pipette tips (including those in
boxes/racks), Combitips Plus tips and Safe-Lock microcentrifuge
tubes, are delivered in their own individually wrapped packaging,
thereby ruling out any danger of contamination.
88
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Product appearance, specifications, and/or prices are subject to change without notice.
Purity levels
Importance of purity factors
Sterility
Importance
In general, a product is defined as being sterile when no living organisms are present
on its surface. However, a more accurate definition of sterility would be “the probability
of one unsterile object being present among a number of sterile objects.”
Sterile products are required whenever the
presence of germs may have a negative
effect; for example, in the prevention of
infection of samples or incorrect test results
for microbiological experiments that would be
caused by unsterile lab equipment.
An adequate indicator for reliable sterilization is a probability value of one in a million
that living microorganisms shall be present on an object following a sterilization
procedure. This is equivalent to an SAL (Sterility Assurance Level) of 10-6, i.e., in a
sample with 106 (1,000,000) microorganisms, one single microorganism survives, or
alternatively, a mass of 106 (1,000,000) objects shall contain one nonsterile object
following sterilization.
For sterilization via irradiation, many directives stipulate a dose of 25 kGy to attain an
SAL of 10-6. This also applies to medicinal products that come into direct contact with
the human body.
Importance
Thermostable substances (glycoproteins) from the outer membrane of bacteria
and other microorganisms can cause fever in humans and impair the growth of cell
cultures.
Absence of pyrogen prevents endotoxin-based
contamination in drug manufacture, cell culture
and medical laboratories.
DNA-free
Importance
DNA is found in all cells of living entities, and it is the carrier of all genetic information.
The highly sensitive PCR technique now enables the detection of individual molecules.
The presence of DNA could lead to falsepositive results for different applications
involving DNA (e.g., additional bands, positive
“negative controls”).
Note: Autoclaving does not remove traces of
DNA.
Free of human DNA
Importance
To eliminate this potential source of contamination, the consumables are tested for
the presence of human DNA. Even a single cell (e.g., skin particles) would be detected
in the test. Manufacturing is virtually fully automated and monitored by staff wearing
protective clothing.
Tests for human DNA prevent consumables from
containing DNA that could lead to false-positive
results (e.g., genetic tests in forensics)
RNase-free
Importance
RNases are enzymes that degrade RNA, the read copy of DNA. These enzymes are
extremely resistant, even to autoclaving and irradiation.
RNase-free products are an absolute must in the
field of molecular biology because RNA is highly
sensitive and can be destroyed very quickly by
RNases.
ATP-free
Importance
ATP is an energy-rich molecule that is part of all living cells; therefore, its presence can
indicate biological contamination.
The test procedure for the quantitative and
qualitative detection of ATP is already an
integral part of hygiene monitoring, e.g., in the
pharmaceutical industry.
Free of PCR inhibitors
Importance
PCR—the replication of DNA—has developed into one of the most important and
commonplace molecular biology methods used in medical diagnostics, genetic
counseling and all basic biological research. However, there are also substances
that impair this reaction, so lab products must be free of these inhibitors.
It is essential that consumables used contain
no impurities that could adversely affect PCR.
This is particularly crucial for the amplification of
minute quantities of genetic substances and for
quantitative PCRs.
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Consumables
Pyrogen- (endotoxin) free
89
Special tubes
Protein LoBind tubes
Description
Protein LoBind tubes are specifically designed for use in proteomics
or other fields of protein research (antibodies, cell biology,
Product features
peptide drugs etc.), where samples tend to be very small and
‡ Minimized protein loss
‡ Available in 0.5 ml,
sample recovery is vital. LoBind tubes improve recovery by reducing
‡ Free of surface coating,
1.5 ml and 2.0 ml sizes
sample-to-tube binding—without the use of any additives or coatings!
e.g., silicone
‡ Safe-Lock lid
A combination of specially developed technology and the purest,
‡ Free of DNA, DNase,
‡ Rated to 18,000 x g
select polypropylene results in tube binding that ensures minimal
RNase and ATP (PCR clean)
‡ High degree of transparency
loss (<3%). Enzymes remain active, and they are not denatured by
‡ Special polypropylene
any tube-surface interactions.
material manufactured
under optimized processing
conditions
Standard
polypropylene
material (PP)
Eppendorf
Siliconized
Protein
Polypropylene
LoBind
Brand A
Brand B
Brand AX
20
(17/14)
18
16
(15/12)
14
12
Protein loss [ mol% ]
Consumables | Sample Preparation
Results: Protein concentration 1 µg/ml
10
8
(5/3)
6
4
(1.3/0.6)
2
(2.0/1.2)
‡ All LoBind tubes are Eppendorf-certified PCR clean and
0
available in a variety of sizes; each box is packaged in bags of
without rinsing
after 1 rinse
‡ Fig. 1: With 131I-labeled BSA, Tris/HCl, pH 7.5, was dissolved in
50 tubes to further reduce the risk of contamination
Ordering information
a solution of 0.1% NaCl and incubated at 20 °C for 24 h in the
Description
Catalog No.
examined tubes; the solutions were removed, the tubes filled
Protein LoBind tubes
PCR clean, 2 bags of 50 = 100 tubes
0.5 ml
1.5 ml
2.0 ml
022431064
022431081
022431102
with the above solution without BSA, and the radioactivity of
the tubes and the solution were then measured; the tubes were
rinsed again, and the radioactivity of the tubes and the solution
were measured once more
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90
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Special tubes
DNA LoBind tubes
Description
DNA LoBind tubes improve recovery by reducing sample-totube binding—without the use of any coatings or additives!
Product features
A combination of specially developed technology and the purest,
‡ Significantly reduced
‡ Special polypropylene
select polypropylene ensures nearly 100% recovery of DNA/RNA
DNA loss:
material manufactured under
from the tube.
– < 0.3% less at standard
optimized conditions
‡ Available in 0.5 ml, 1.5 ml
All LoBind tubes are Eppendorf-certified PCR clean and available
and 2.0 ml sizes
in a variety of sizes. Each box is packaged in bags of 50 tubes to
concentratons
‡ Safe-Lock lid
further reduce the risk of contamination.
(e.g., for precipitation)
‡ Rated to 30,000 x g
reaction conditions
– < 1% less at high-salt
‡ Free of surface coating,
(except 2.0 ml)
e.g., silicone
‡ High degree of transparency
‡ Free of DNA, DNase,
RNase and ATP
A) Standard conditions (low-salt concentration)
DNA LoBind
(Eppendorf)
Other
brands
1,0
Eppendorf
standard
material
DNA LoBind
(Eppendorf)
Other
brands
100
B
(0.40/1.19)
B
(75/95)
(96/70)
0,8
80
(0.22/0.61)
AX
(0.30/0.51)
0,4
60
DNA loss [ mol% ]
DNA loss [ mol% ]
0,6
(0.1/0.3)
0,2
0
40
AX
(1.8/22.5)
20
(0.7/1.0)
0
20 °C, 24 h
95 °C, 10 min
‡ Fig. 2A: DNA (830 bp) was labeled with 32P and dissolved in
37 °C, 24 h
95 °C, 10 min
Ordering information
0.1 M NaCI/TE buffer, pH 7.5 (1.0 ng/μl), and then incubated in
Description
Catalog No.
the tubes at 20 °C for 24 h or at 95 °C for 10 min
DNA LoBind tubes
PCR clean, 5 bags of 50 = 250 tubes
0.5 ml
1.5 ml
2.0 ml
022431005
022431021
022431048
‡ Fig. 2B: DNA (130 bp) was labeled with 32P at 5’ end and
dissolved in 2.5 M NaCI/TE buffer, pH 7.5; the solution (0.2 ng/μl)
was incubated in the tubes at 37 °C for 24 h or at 95 °C for 10 min;
and following removal of the solutions, the radioactivity of the
Sample Preparation | Consumables
Eppendorf
standard
material
B) High-salt concentration (e.g., precipitation)
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empty tubes was measured and recorded
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
91
Special tubes
Safe-Lock microcentrifuge tubes
Product features
‡ A small hook on the hinged
‡ Excellent mechanical
lid clips around the rim of the
stability during centrifugation:
test tube
– Up to 30,000 x g for 0.5 ml
– The lid hook prevents
the tube from opening
accidentally
Consumables | Sample Preparation
(e.g., during heating)
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and 1.5 ml tubes
– Up to 25,000 x g for
2.0 ml tubes
– 70,000 x g possible in
‡ Tubes can be autoclaved
form-fitting rotor
when open (121 °C, 20 min)
‡ Graduation scale
‡ Can be easily and
‡ Available in Eppendorf
single-handedly opened
Quality, Eppendorf Biopur and
‡ Now even safer! Safe-Lock tubes (0.5 ml and 1.5 ml only)
‡ Frosted labeling surfaces
PCR clean purity levels
are rated up to 30,000 x g
Description
The hinged lid on Eppendorf Safe-Lock microcentrifuge tubes
eliminates any danger of leaks. Safe-Lock tubes ensure absolute
safety when working with expensive or toxic samples, radioactive
substances or DNA. All tubes have volume markings and
convenient labeling surfaces.
‡ A needle placed through
the thin membrane in the
More information on PCR tubes and
plates begins at page 197.
middle of the lid allows the
aerosol-free removal of
biohazardous substances
Ordering information
Catalog No.
Catalog No.
Catalog No.
Volume
0.5 ml
1.5 ml
2.0 ml
Safe-Lock microcentrifuge tubes
per 500 pcs.
per 500 pcs.
per 500 pcs.
Colorless
022363611
022363204
022363352
Amber (light protection)
022363638
022363221
022363379
Blue
022363646
022363247
022363395
Green
022363654
022363263
022363417
Red
022363662
022363280
022363433
Yellow
022363671
022363301
022363450
Assorted colors*
022363697
022363328
022363476
Eppendorf Biopur purity
per 50 pcs.
per 100 pcs.
per 100 pcs.
Individually wrapped
022600001
022600028
022600044
PCR clean purity
per 500 pcs.
per 500 pcs.
per 500 pcs.
DNA-, DNase-, RNase-, PCR inhibitor-free
022363719
022363212
022363344
*100 each: Colorless, blue, green, red and yellow.
92
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Special tubes
Flex-Tubes®
Product features
Description
‡ Economically priced
‡ Volume graduations
The easy-open, easy-close design of our Flex-Tubes
‡ Tubes can be autoclaved
‡ Flex-Tubes are available
provides convenient and reliable sample preparation,
when open (121 °C, 20 min)
in Eppendorf Quality and
centrifugation and storage.
‡ Etched marking surfaces
PCR clean purity levels
‡ Rated to 25,000 x g for
excellent mechanical stability
during centrifugation
Ordering information
Description
Catalog No.
Flex-Tubes, 1.5 ml, 500 pcs.
022363514
Blue
022363531
Green
022363557
Red
022363573
Yellow
022363590
Colorless
022364111
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Sample Preparation | Consumables
Amber
Micropestle
Product feature
‡ Autoclavable micropestle for resuspending pellets from
1.5 ml to 2.0 ml test tubes
Ordering information
Description
Catalog No.
Micropestle,
for resuspending pellets from
1.5 ml to 2.0 ml tubes, set of 10
022365622
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
93
Special tubes
Find the right tube
Ordering information
Consumables | Sample Preparation
Description
0.5 ml
Safe-Lock
Product features
‡ Polypropylene tubes of high chemical
resistance
‡ Flat, frosted Safe-Lock lid
‡ Thin membrane in the lid
‡ Frosted labeling surfaces
‡ Rated to 30,000 x g
‡ Autoclavable (121 °C, 20 min)
‡ Polypropylene tubes of high chemical
resistance
‡ Flat, frosted Safe-Lock lid
‡ Thin membrane in the lid
‡ Frosted labeling surfaces
‡ Rated to 30,000 x g
‡ Autoclavable (121 °C, 20 min)
Type (purity/color)
Quantity
(pcs.)
Catalog No.
Quantity
(pcs.)
Catalog No.
Standard Quality
Colorless
500
022363611
500
022363204
Amber (light protection)
500
022363638
500
022363221
Blue
500
022363646
500
022363247
Green
500
022363654
500
022363263
Red
500
022363662
500
022363280
Yellow
500
022363671
500
022363301
Assorted colors*
500
022363697
500
022363328
Eppendorf Biopur
50
022600001
100
022600028
PCR clean
500
022363719
500
022363212
Protein LoBind (rated to 18,000 x g)
100
022431064
100
022431081
DNA LoBind
250
022431005
250
022431021
Permanent, waterproof marker
5
022431242
®
*100 each: Colorless, blue, green, red and yellow.
Eppendorf Biopur
Product features
‡ Sterile
‡ Individually wrapped
‡ Free of pyrogen, RNase,
‡ Certificates available at
DNA and ATP
www.eppendorf.com
‡ Monitored and certified by
an external lab
94
1.5 ml
Safe-Lock
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Special tubes
Find the right tube
2.0 ml
Safe-Lock
1.5 ml
Flex-Tube®
‡ Polypropylene tubes of high chemical
resistance
‡ Flat, frosted lid
‡ Frosted labeling surfaces
‡ Volume markings
‡ Rated to 25,000 x g
‡ Autoclavable (121 °C, 20 min)
Quantity
(pcs.)
Catalog No.
Quantity
(pcs.)
Catalog No.
500
022363352
500
022364111
500
022363379
500
022363514
500
022363395
500
022363531
500
022363417
500
022363557
500
022363433
500
022363573
500
022363450
500
022363590
500
022363476
100
022600044
500
022363344
500
022364120
100
022431102
250
022431048
Sample Preparation | Consumables
‡ Polypropylene tubes of high chemical
resistance
‡ Flat, frosted Safe-Lock lid
‡ Thin membrane in the lid
‡ Frosted labeling surfaces
‡ Volume markings
‡ Rated to 25,000 x g
‡ Autoclavable (121 °C, 20 min)
Eppendorf PCR clean
Product features
‡ Free of human DNA and
‡ Certificates available at
PCR inhibitors
www.eppendorf.com
‡ Free of DNase and RNase
‡ Produced and packaged in
clean-room environment
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
95
Deepwell plates
Eppendorf Plate™ Deepwell 96 and 384
Application
‡ For processing, transport and storage of liquids
Product features
‡ RecoverMax™ design—
‡ High chemical resistance
rounded-corner,
‡ Autoclavable
conical bottom well
(121 °C, 20 min)
‡ Frames feature
‡ Suitable for automation
alphanumeric coding for
‡ Conforms to SBS*
easy well identification
standards
‡ g-Safe—stable at high
‡ Optional bar coding
centrifugation
available
‡ Raised well rims for effective
sealing
Description
Consumables | Sample Preparation
Eppendorf sets the new standard for deepwell plates with new
96
RecoverMax well designs. The well geometry enables high-speed
pipetting, excellent mixing and high-speed centrifugation to
accelerate your manual as well as your automated work processes.
Sophisticated colors and coding make reading, orientation and
manual handling easier and faster.
Made of virgin polypropylene, each plate type is available as
Standard and Sterile quality. Eppendorf Plate Deepwell 96/500,
96/1,000 and 384/200 are also available as DNA LoBind and
Protein LoBind quality. Ease of stacking, multiple sealing options
as well as high chemical and temperature resistance make these
plates excellent, multipurpose tools. The plates fulfill current
guidelines: SBS*, IVD, EN ISO 9001/13485. Certificates are
‡ Available in five frame colors
available on request at www.eppendorf.com
*Society for Biomolecular Screening
‡ RecoverMax design: rounded-corners with conical
‡ OptiTrack matrix: frames feature alphanumeric coding for
bottom wells
easy well identification
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Deepwell plates
Eppendorf Plates™ Deepwell 96 and 384
g-Safe: Reliable centrifugation stability
Easy and safe sealing:
Eppendorf Plates Deepwell 96 and 384 are designed to be
Eppendorf Plates Deepwell 96 and 384 are high-quality consumables
centrifugation-stable and durable in a temperature range from
to ease high-throughput operation and handling. They are warp-
4 °C to 40 °C. Standard, DNA and Protein LoBind Eppendorf Plate
resistant, always revealing a planar surface during each handling
Deepwell 96 and 384 can be centrifuged up to 6,000 x g1. Sterile
step; this planarity, together with precisely molded raised rims,
Eppendorf Plate Deepwell 96 and 384 are centrifugation stable
leads to perfectly sealed wells, even after several sealing procedures.
up to 5,000 x g1. For sample recovery after processing or storage,
Other benefits include reduction of cross-contamination and
Eppendorf Centrifuge 5804 R and 5810 R and rotors A-4-81-MTP
minimized sample loss during storage. Sealing can be done
and A-2-DWP are recommended (see pages 146–147, 150, 154
by adhesive seal, heat-sealing or sealing mats.
and 176).
entrifugation stability of each plate type generally depends on the plate itself, centrifuge,
C
rotor, rotor bucket, adapter, max. rpm/max. g-force, operation temperature, operation time,
liquid density, organic solvent, cumulative maximum weight and correct handling.
Please read instruction manual prior to use.
1
Recommended sealing options for Eppendorf Plates Deepwell 96 and 384
Eppendorf Plate
Heat sealing
Sealing mats
• Self-adhesive
• Certified PCR clean
• Transparent
• Reusable
Deepwell 96/2,000
Eppendorf PCR Film
Eppendorf PCR Foil
Eppendorf Heat Sealing Film
Eppendorf Sealing Mats
Deepwell 96/1,000
Eppendorf PCR Film
Eppendorf PCR Foil
Eppendorf Heat Sealing Film
Eppendorf Sealing Mats
Deepwell 96/500
Eppendorf PCR Film
Eppendorf PCR Foil
Eppendorf Heat Sealing Film
Eppendorf Sealing Mats
Deepwell 384/200
Eppendorf PCR Film
Eppendorf PCR Foil
Eppendorf Heat Sealing Film
Eppendorf Sealing Mats2
Sealing mats from other suppliers may also be used.
2
Ordering information
Description
Catalog No.
Eppendorf PCR Film (self-adhesive), 100 pcs.
951023019
Eppendorf PCR Foil (self-adhesive), 100 pcs.
951023001
Eppendorf Heat Sealing Film, 10 x 10 pcs.
951023060
Eppendorf Sealing Mats
For Deepwell 96/2,000, 50 pcs.
951030147
For Deepwell 96/1,000 and 96/500), 50 pcs.
951030121
Heat Sealer, 115 V/50 Hz
951023078
Sample Preparation | Consumables
Adhesive seal
‡ Available in five frame colors
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
97
Deepwell plates
Eppendorf Plates™ Deepwell 96 and 384
Ordering information
Description
Quality
Color
Packaging
Catalog No.
Standard
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031003
951031020
951031046
951031062
951031089
Sterile
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031101
951031127
951031143
951031160
951031186
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031208
951031224
951031241
951031267
951031283
Protein LoBind
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031305
951031321
951031348
951031364
951031381
Standard
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951031402
951031429
951031445
951031461
951031488
Sterile
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951031500
951031526
951031542
951031569
951031585
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951031607
951031623
951031640
951031666
951031682
Protein LoBind
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951031704
951031721
951031747
951031763
951031780
Deepwell plate 384/200 µl
Regular package
Consumables | Sample Preparation
@RPQLJ
M>@H>DFKD
>S>FI>?IB
Large package
All Deepwell plates are available with bar code upon request
‡ Available in five frame colors
98
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Deepwell plates
Eppendorf Plates™ Deepwell 96 and 384
Ordering information
Description
Quality
Color
Packaging
Catalog No.
Standard
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031801
951031828
951031844
951031861
951031887
Sterile
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951031909
951031925
951031941
951031968
951031984
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951032000
951032026
951032042
951032069
951032032
Protein LoBind
White
Yellow
Red
Green
Blue
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
40 plates (5 bags of 8)
951032101
951032123
951032140
951032166
951032182
Standard
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951032204
951032221
951032247
951032263
951032280
Sterile
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951032301
951032328
951032344
951032361
951032387
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951032409
951032425
951032441
951032468
951032484
Protein LoBind
White
Yellow
Red
Green
Blue
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
120 plates (10 bags of 12)
951032506
951032522
951032549
951032565
951032581
Deepwell plate 96/500 µl
Regular package
Sample Preparation | Consumables
Large package
@RPQLJ
M>@H>DFKD
>S>FI>?IB
All Deepwell plates are available with bar code upon request
‡ Available in five frame colors
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
99
Deepwell plates
Eppendorf Plates™ Deepwell 96 and 384
Ordering information
Description
Quality
Color
Packaging
Catalog No.
Standard
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951032603
951032620
951032646
951032662
951032689
Sterile
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951032701
951032727
951032743
951032760
951032786
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951032808
951032824
951032841
951032867
951032883
Protein LoBind
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951032905
951032921
951032948
951032964
951032981
Standard
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033206
951033022
951033049
951033065
951033081
Sterile
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033103
951033120
951033146
951033162
951033189
DNA LoBind
(also for RNA
and other
nucleic acids)
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033201
951033227
951033243
951033260
951033286
Protein LoBind
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033308
951033324
951033341
951033367
951033383
Deepwell plate 96/1,000 µl
Regular package
Consumables | Sample Preparation
@RPQLJ
M>@H>DFKD
>S>FI>?IB
Large package
All Deepwell plates are available with bar code upon request
‡ Available in five frame colors
100
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Deepwell plates
Eppendorf Plates® Deepwell 96 and 384
Ordering information
Description
Quality
Color
Packaging
Catalog No.
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951033405
951033421
951033448
951033464
951033481
Sterile
White
Yellow
Red
Green
Blue
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
20 plates (5 bags of 4)
951033502
951033529
951033545
951033561
951033588
Standard
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033600
951033626
951033642
951033669
951033685
Sterile
White
Yellow
Red
Green
Blue
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
80 plates (10 bags of 8)
951033707
951033723
951033724
951033766
951033782
Deepwell plate 96/2,000 µl
Regular package
Large package
Standard
Technical specifications
H
W
D
Description
Deepwell 384/200 µl
Deepwell 96/500 µl
Deepwell 96/1,000 µl
Deepwell 96/2,000 µl
Number of wells:
384
96
96
96
Work volume:
20–225 μl
30–550 μl
30–1,000 μl
50–2,000 μl
Total well volume:
240 μl
700 μl
1,200 μl
2,400 μl
Well-bottom shape:
Sample Preparation | Consumables
All Deepwell plates are available with bar code upon request
@RPQLJ
M>@H>DFKD
>S>FI>?IB
Well shape:
,
,
,,
rounded edge
Dimensions (W x D x H):
5.03 x 3.37 x 0.99 in
127.8 x 85.5 x 25.1 mm
rounded edge
5.03 x 3.37 x 1.07 in
127.8 x 85.5 x 27.1 mm
5.03 x 3.37 x 1.74 in
127.8 x 85.5 x 44.1 mm
5.03 x 3.37 x 1.74 in
127.8 x 85.5 x 44.1 mm
More information at www.eppendorf.com
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
101
Rack systems
Racks and holders for Eppendorf® microcentrifuge tubes
Product features of Tube Racks
Description
‡ 24-place capacity
‡ Numbered positions
Eppendorf Tube® Racks, ideal for sample processing and the short-
‡ Unique swivel connectors
‡ UV-resistant
or medium-term storage of samples, can be stored in a freezer and
interlock multiple racks in
‡ Autoclavable
withstand temperatures as low as –80 °C. Each tube is properly
front-to-back and side-to-side
(121 °C, 20 min)
spaced and elevated for easy access and convenient handling.
configurations
Tube Racks are available for 0.5 ml, 1.5 ml and 2.0 ml tubes.
They can be stacked, even when fully loaded, interlocked to other
racks on both sides and feature labeling surfaces to enable easy
Consumables | Sample Preparation
identification. The racks are UV-resistant and fully autoclavable.
Product features of Eppendorf Rack 3821
‡ 20-place capacity for
Note: This product should not
1.5/2.0 ml tubes
be autoclaved.
‡ Numbered positions
‡ Economically priced
Ordering information
102
Description
Catalog No.
Tube Racks, 24-place capacity
For 0.5 ml tubes
For 1.5/2.0 ml tubes
022364243
022364227
Rack 3821, 20-place capacity
For 1.5/2.0 ml tubes
022364201
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
MixMate™, Thermomixer® R, Thermomixer and ThermoStat plus
!
NEW
MixMate
Thermomixer R
Thermomixer
ThermoStat plus
Heats
Heats
Heats
Features
Cools
Mixes
Cools
Mixes
Mixes
From 13 °C below RT to 99 °C
From 4 °C above RT to 99 °C
From 30 °C below RT to 99 °C
•E
nzyme reactions
(e.g., DNA restriction
digestion, cDNA production
via reverse transcription,
Proteinase K digestion)
• Enzyme reactions at
temperatures 4 °C above RT
(e.g., DNA restriction
digestion, cDNA production
via reverse transcription,
Proteinase K digestion)
• Enzyme reactions
(e.g., DNA restriction
digestion, cDNA production
via reverse transcription,
Proteinase K digestion)
Temperature control range
No temperature control
available
Application examples
• Mixing PCR preparations
• Incubation
(e.g., ELISA assays)
• Colorimetric assays
(e.g., Bradford, Lowry, BCA)
• Reporter gene assays
(e.g., βb-galactosidase,
luciferase)
• Mixing restriction digestion
preparations
• Vortexing numerous
tube formats
(e.g., 15 and 50 ml
conical tubes)
• And much more
• Transformation
• Denaturation of DNA,
RNA, proteins
• Cultivation of bacteria
• Cultivation of yeasts
• Bead technology
(e.g., protein purification)
• Isolation of DNA fragments
from agarose gels
• Plasmid isolation
• Immunoprecipitation
• Hybridization experiments
on slides, e.g., microarrays or
in situ hybridization
• Transformation
• Transformation
• Denaturation of DNA,
RNA, proteins
• Cultivation of bacteria
• Cultivation of yeasts
• Bead technology
(e.g., protein purification)
• Isolation of DNA fragments
from agarose gels
• Plasmid isolation
• And much more
• Denaturation of DNA,
RNA, proteins
• Storage of enzymes,
nucleic acids, etc., at
defined temperatures
• Cell lysis
• In vitro translation
• Ligation
• Hybridization experiments on
slides, e.g., microarrays or in
situ hybridization
Sample Preparation | Instruments
• Resuspending pellets
(e.g., bacteria, DNA,
cell culture pellets)
• Gentle thawing of deep-frozen
solutions such as buffers
• And much more
• Gentle thawing of deep-frozen
solutions such as buffers
• And much more
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
103
Temperature control and mixing
!
NEW
MixMate™
Description
Meet our compact and amazingly versatile mixer, specially
designed for mixing small volumes in numerous plate and tube
Instruments | Sample Preparation
Product features
104
formats. Mixmate’s fast and precisely controlled mixing movements
‡ 2DMix-Control: efficient
‡ Tube holders: enable mixing
(2DMix-Control) enable efficient and homogenous mixing through
and very fast mixing of
of micro test tubes up to 2 ml
all sample vessels, and the device may be used for virtually any
volumes from 5 μl to 2 ml
under controlled conditions
application. It accommodates micro test tubes, PCR plates,
‡ 3-in-1 format: mixing of
(0.2 ml PCR tubes and PCR
deepwell plates and MTPs. Mixmate also features a touch vortex
plates, tubes and vortexing
strips and 0.5 ml, 1.5 ml and
function with an innovative 2-second override to provide an
function
2.0 ml micro test tubes)
ergonomic work process and continuous vortexing.
‡ Anti-spill technology:
‡ Preprogrammed direct
controlled mixing movement
selection keys: optimal time/
prevents spilling and
frequency for each plate or
lid wetting
tube format
‡ Universal holder: allows
‡ Vortexing numerous tube
mixing of all plate formats
formats (e.g., 15 ml and 50 ml
(up to 96- and 384-wells):
conical tubes)
MTPs, deepwell plates, PCR
‡ Automatic imbalance
plates (skirted, semiskirted,
detection
unskirted)
‡ Robust design and
See application list on previous page.
Also visit the MixMate web site: www.eppendorf.com/mixmate
small footprint
‡ User-friendly operation
‡ IVD conformity
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
!
NEW
MixMate™
‡ Deepwell plates
‡ MTPs
‡ Test tubes and
PCR tubes/strips
Technical specifications
Mixing frequency:
300 to 3,000 rpm (in 50 rpm increments)
Mixing and vortexing radius:
1.5 mm (3 mm mixing stroke)
Touch vortexing frequency:
3,500 rpm
Adjustable mixing time:
15 s to 99.5 h; continuous
Noise level:
< 50 dB(A)
Power supply:
100–120 V, 50–60 Hz
Power consumption:
40 W
Dimensions (W x D x H):
6.7 x 9.1 x 5.1 in/17 x 23 x 13 cm
Weight:
9.25 lb/4.2 kg
Sample Preparation | Instruments
‡ PCR plates
Ordering information
Description
Catalog No.
MixMate
Includes 3 tube holders: PCR 96, 0.5 ml, 1.5/2.0 ml
022674200
Accessories
Tube Holder PCR 96, for 96 x 0.2 ml PCR tubes,
PCR strips or 96-well PCR plates, semiskirted or unskirted
022674005
Tube Holder 0.5 ml, for 24 x 0.5 ml micro test tubes
022674021
Tube Holder 1.5/2.0 ml, for 24 x 1.5 or 2.0 ml micro test tubes
022674048
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
105
Temperature control and mixing
Thermomixer®
Description
Small and perfectly designed: Thermomixer is the perfect compact
unit for 24 x 1.5 ml microcentrifuge tubes. Its impressive features
Product features
include an extremely small footprint, a straightforward design and
‡ Dedicated for 24 x 1.5 ml
‡ Small footprint
microcentrifuge tubes
‡ Quiet operation due to
‡ Short-mix function
perfect fit of the test tubes
‡ Includes an IsoTherm rack
in the thermoblock
high stability—even at maximum mixing frequency.
and an IsoTherm cooling pack
for sample storage at 0 °C
(see page 115)
Instruments | Sample Preparation
See application list on page 103.
106
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
Thermomixer®
Technical specifications
Temperature control range:
From approx. 4 °C above RT to 99 °C
Temperature settings:
From 1 °C to 99 °C
Mixing frequency:
300–1,400 rpm
Mixing orbit:
3 mm
Incubating accuracy:
± 1 °C between 20 °C and 45 °C
± 2 °C below 20 °C and above 45 °C
Heat-up speed:
Approx. 5 °C/min
Power supply:
100–240 V, 50–60 Hz
Power requirement:
90 W
Dimensions (W x D x H):
6.5 x 9.4 x 5.7 in/16.5 x 24 x 14.5 cm
Weight:
5.3 lb/2.4 kg
Ordering information
Description
Catalog No.
For 1.5 ml tubes, includes work rack and 0 °C cool pack, 120 V/50–60 Hz
022670000
For 1.5 ml tubes, includes work rack and 0 °C cool pack, 220 V/50–60 Hz
022670051
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Instruments
Thermomixer with thermoblock
107
Temperature control and mixing
Thermomixer® R
Description
The Thermomixer R conveniently offers simultaneous mixing
and temperature control. It meets even the highest demands for
Product features
user-friendliness and flexibility: its counter-cooling ability provides
‡ Wide temperature control
‡ Interval mixing
efficient cooling, thereby greatly expanding both its temperature
and application ranges thanks
‡ Pause function
control functions and range of applications.
to active Peltier element
‡ RS-232 interface for remote
heating and counter-cooling
control
Ten different thermoblocks are available, all of which can be simply
‡ Excellent mixing and
‡ Ten different exchangeable
and quickly exchanged, to adapt the unit to daily and/or changing
incubating performance
thermoblocks offer a high
requirements in the laboratory. Two freely selectable program
‡ Selectable “time” and
degree of flexibility
sequences with incubating, mixing and rest phases, as well as
“temp” controls: timer function
‡ Automatic thermoblock
subsequent cooling to 13°C below room temperature, make
starts with program activation
identification and display
everyday lab routines much easier.
or when set temperature is
of the maximum revolutions
reached
‡ Quiet operation due to
The Eppendorf Thermomixer R is your reliable partner for short
‡ Short-mix function
perfect fit of the test tubes
reaction times and efficient lab routines.
Instruments | Sample Preparation
in the thermoblock
108
Exchangeable thermoblocks ordering information on page 113;
see application list on page 103.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
Thermomixer R
Technical specifications
Temperature control range:
From 13 °C below RT to 99 °C*
Temperature settings:
From 1 °C to 99 °C
Mixing frequency:
300–1,500 rpm*
Incubating accuracy:
±0.5 °C between 20 °C and 45 °C
±2 °C below 20 °C and above 45 °C
Heat-up speed:
Approx. 5 °C/min
Cool-down speed:
2–3 °C/min between 99 °C and RT
0.5–1 °C/min between RT and 13 °C below RT
Timer:
1 min to 99:59 h, continuous
Mixing orbit:
3 mm
Power supply:
100–240 V, 50–60 Hz
Power requirement:
90 W
Interface:
RS-232
Dimensions, without block (W x D x H):
8.7 x 9.8 x 4.9 in/22 x 25 x 12.5 cm
Weight, without block:
7.0 lb/3.2 kg
*Upper limit depends on thermoblock chosen (thermoblock options on page 113)
Description
Catalog No.
Thermomixer R
Without block, 120 V/50–60 Hz
Without block, 220 V/50–60 Hz
022670107
022670158
Exchangeable thermoblocks and accessories, for
24 microcentrifuge tubes, complete with rack and cool pack for 0 °C (from IsoTherm system)
0.5 ml
1.5 ml
2.0 ml
022670506
022670522
022670549
24 test tubes, diameter 11–11.9 mm, height 30–76 mm
022670581
24 Cryotubes , 1.5/2.0 ml, external diameter max. 12.5 mm, all base shapes
022670557
Falcon® tubes
8 x 15 ml
4 x 50 ml
022670531
022670514
®
4 slides, for hybridization experiments
022670590
Slides DC, holds 4 Eppendorf DualChip slides, with clamping plate
022670786
MTPs and deepwell plates, with lid
022670565
Adapter plate for 96 x 0.2 ml PCR tubes and PCR plates 96,
to insert in exchangeable thermoblock for MTPs
022670573
®
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Instruments | Consumables
Ordering information
109
Temperature control and mixing
ThermoStat plus
Description
Convenient and accurate temperature control for all commonly
used lab tubes is the concept behind our ThermoStat plus.
Product features
The result is a compact benchtop thermostat with Peltier
‡ Precise temperature control
‡ The run time for the linear
technology. Its most notable strength is its ability to maintain
with active cooling and heating
transition between two
different temperature levels: it can heat up samples to 99 °C
‡ Choice of 11 different
temperature levels can be
and also cool them down to as low as –5 °C.
exchangeable thermoblocks
individually programmed
‡ Temperature control range
‡ User-friendly operation
Exchangeable thermoblocks ensure effective heat transfer and
from –5 °C to 99 °C
‡ RS-232 interface for remote
the flexibility to adapt the ThermoStat plus to your particular
‡ Individual programming of
control and documentation
application. Operation is incredibly easy: simply select the
up to four separate time
‡ Pause function
temperature and press “Start” to initiate the timer. Both the
phases and temperature ramps
temperature and run time can be programmed for special
applications.
Instruments | Sample Preparation
Exchangeable thermoblocks for ThermoStat plus ordering
information on page 113; see application list on page 103.
110
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
ThermoStat plus
Technical specifications
Temperature control range:
From 30 °C below RT to 99 °C*
Temperature settings:
From –5 °C to 99 °C
Incubating accuracy:
±1.0 °C at 0 °C
±0.5 °C at 37 °C
±1.0 °C at 90 °C
Heat-up speed:
5 °C/min
Cool-down speed:
6 °C/min between 99 °C and 25 °C
1.5 °C/min between 25 °C and –5 °C
Timer:
1 min to 99:59 h, continuous
Power supply:
100–240 V, 50–60 Hz
Power requirement:
75 W
Interface:
RS-232
Dimensions, without block (W x D x H):
8.7 x 9.8 x 4.9 in/22 x 25 x 12.5 cm
Weight, without block:
11 lb/5 kg
*Upper limit depends on thermoblock chosen (thermoblock options on page 113)
Description
Catalog No.
ThermoStat plus
Without block, 120 V/50–60 Hz
022670204
Exchangeable thermoblocks and accessories, for
24 microcentrifuge tubes, complete with rack and cool pack for 0 °C (from IsoTherm system)
0.5 ml
1.5 ml
2.0 ml
022670506
022670522
022670549
24 test tubes, diameter 11–11.9 mm, height 30–76 mm
022670581
24 Cryotubes®, 1.5/2.0 ml, external diameter max. 12.5 mm, all base shapes
022670557
Falcon tubes
8 x 15 ml
4 x 50 ml
022670531
022670514
4 slides, for hybridization experiments
022670590
Slides DC, holds 4 Eppendorf DualChip® slides, with clamping plate
022670786
CombiBox, for ThermoStat plus, accommodates various size test tubes and flasks,
interior 13.5 x 9.0 x 6.0 cm (W x D x H)
022670603
MTPs and deepwell plates, with lid
022670565
Adapter plate for 96 x 0.2 ml PCR tubes and PCR plates 96,
to insert in exchangeable thermoblock for MTPs
022670573
®
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Instruments | Consumables
Ordering information
111
Temperature control and mixing
Exchangeable thermoblocks for Thermomixer® and ThermoStat plus
Description
The exchangeable thermoblock system for the Thermomixer R
and ThermoStat plus ensures a precise “fit” for all common lab
Product features
tubes: from simple temperature control of a chemical to complex
‡ Contact-safe thanks
‡ Efficient and simple
enzyme reactions and hybridization experiments, and from MTPs
to optimally shielded
exchange of all thermoblocks
to 50 ml conical tubes—the application possibilities are virtually
thermoblocks
‡ Tubes easily accessible
endless!
‡ Better laboratory
‡ Personal benchtop
hygiene—eliminates use of
devices enable you to always
water baths: reduced risk of
keep an eye on your samples
contamination
Instruments | Sample Preparation
D
C
B
E
A
G
F
H
J
I
‡ Exchangeable thermoblocks accommodate:
A. 1–4 Eppendorf DualChip® slides, with clamping plate
B. MTPs and deepwell plates
H.Test tubes with a diameter of 11–11.9 mm and
a height of 30–76 mm
C. 4 x 50 ml Falcon® tubes
I. 24 x 0.5 ml, 1.5 ml and 2.0 ml microcentrifuge tubes*
D. 8 x 15 ml Falcon tubes
J. 1–4 standard glass slides
E.Exclusively for ThermoStat plus: CombiBox provides universal
temperature control (e.g., for the preparation/cooling of
enzymes) for flasks and beakers with a height of ≤ 60 mm
*Includes an IsoTherm rack and IsoTherm cool pack for 0 °C. For more information see page 115.
F.Adapter plate for 96 x 0.2 ml PCR tubes and PCR plates 96
easily inserts into exchangeable thermoblock for MTPs (see B)
G. 24 x 1.5–2 ml Cryotubes®
112
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
Exchangeable thermoblocks for Thermomixer® R and ThermoStat plus
Technical specifications
Exchangeable thermoblock for
Max. Temp.
Max. rpm
Thermomixer R
ThermoStat plus
24 x 0.5 ml microcentrifuge tubes
99 °C
24 x 1.5 ml microcentrifuge tubes
99 °C
1,500
‡
‡
1,400
‡
‡
24 x 2.0 ml microcentrifuge tubes
24 test tubes, dia. ≤ 11.9 mm, height 30–76 mm
99 °C
1,400
‡
‡
99 °C
1,400
‡
‡
24 x 1.5–2 ml Cryotubes
99 °C
1,400
‡
‡
8 x 15 ml Falcon® tubes
99 °C
750
‡
‡
4 x 50 ml Falcon tubes
99 °C
750
‡
‡
MTPs and deepwell plates, PCR tubes and plates
70 °C
1,400*
‡
‡
4 slides
99 °C
1,400
‡
‡
Slides DC
70 °C
1,400
‡
‡
CombiBox (ThermoStat plus only)
95 °C
0
®
‡
*With a load of, e.g., 200 g, the max. rpm is 800.
Ordering information
Catalog No.
Exchangeable thermoblocks and accessories, for
24 microcentrifuge tubes, complete with rack and cool pack for 0 °C (from IsoTherm system)
0.5 ml
1.5 ml
2.0 ml
022670506
022670522
022670549
24 test tubes, diameter 11–11.9 mm, height 30–76 mm
022670581
24 Cryotubes, 1.5/2.0 ml, external diameter max. 12.5 mm, all base shapes
022670557
Falcon® tubes
8 x 15 ml
4 x 50 ml
022670531
022670514
4 slides, for hybridization experiments
022670590
Slides DC, holds 4 Eppendorf DualChip® slides, with clamping plate
022670786
CombiBox, for ThermoStat plus, accommodates various size test tubes and flasks,
interior 13.5 x 9.0 x 6.0 cm (W x D x H)
022670603
MTPs and deepwell plates, with lid
022670565
Adapter plate for 96 x 0.2 ml PCR tubes and PCR plates 96,
to insert in exchangeable thermoblock for MTPs
022670573
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Instruments | Consumables
Description
113
Temperature control and mixing
Exchangeable thermoblocks for slides and microarrays
Application
Product features
‡ Hybridization experiments using slides for
‡ Exchangeable thermoblocks
‡ High level temperature
– Microarray hybridization
for 1 to 4 slides (standard
stability during hybridization
– in situ hybridization (e.g., FISH, CISH)
object holder format) or
‡ System ensures the
– Immunohistochemistry
Eppendorf DualChips
highest level of homogeneity
‡ Uniform heating and
of the hybridization parameters
Description
atmospheric control improves
between the chambers
Our exchangeable thermoblocks are ideal for hybridization
signal-to-background ratio,
‡ Reservoir pads ensure
experiments using 1 to 4 slides (e.g., microarrays, FISH, CISH,
increases signal intensity
high air humidity
Eppendorf DualChip® microarrays). The exchangeable thermoblock
and results in excellent
‡ Light-proof interior
for 4 slides can be used for any application with standard glass
reproducibility
‡ The pressure plate of the
slides, whereas the exchangeable thermoblock for slides DC was
‡ Increased hybridization
exchangeable thermoblock
especially designed for Eppendorf DualChips, our specific gene
efficiency due to rapid and
for slides DC ensures optimal
expression slides (page 206).
homogeneous mixing of
hybridization signals when
the sample (when using
using Eppendorf DualChips
Instruments | Sample Preparation
the Thermomixer® R)
Technical specifications
Slide dimensions
Length:
Width:
Height:
75.0–76.5 mm
25.0–26.0 mm
0.9–1.2 mm
Rotational speed:
Maximum 1,400 rpm
Temperature control:
Maximum 99 °C (thermoblock
for slides DC: max. 70 °C)
Temperature homogeneity:
≤ 2 °C* (at 70 °C)
≤ 0.5 °C* (at 45 °C)
Heat-up speed:
> 5 °C / min*
Ordering information
Description
Exchangeable thermoblock
For 4 slides, for hybridization
experiments
022670590
For slides DC,
holds 4 Eppendorf DualChip
slides, with clamping plate
022670786
*Values were determined using the Thermomixer R.
114
Catalog No.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Temperature control and mixing
IsoTherm system
Application
Description
‡ Cooling, transporting and storing deep-frozen samples
The IsoTherm* system, which consists of an IsoRack (work rack),
IsoSafe (insulating box) and two IsoPacks (cool packs), specializes
in cooling samples effectively and consistently at –21 °C or 0 °C
over many hours. The IsoTherm system can be used to prevent
Product features
enzymes from becoming inactive as well as for PCR sample
‡ Holds 24 reaction tubes
‡ All system components
(e.g., 0.5 ml or 1.5/2.0 ml)
can be used separately
‡ IsoPack (cold pack):
‡ Racks are stackable,
white maintains 0 °C for up to
autoclavable and floatable;
6 hours; and blue maintains
can be centrifuged in
-21 °C for up to 3 hours
an MTP rotor (page 154)
preparation, sample transport and slow thawing of frozen samples.
*U.S. Pat. 6,616,897
IsoRacks with proper support adapters can be
centrifuged up to 2,700 x g, see page 156.
‡ No more melting ice baths
(reduces risk of contamination)
‡ IsoRack with 0 °C IsoPack
‡ –21 °C IsoPack
‡ IsoSafe with IsoRack and –21 °C IsoPack
Ordering information
Description
Catalog No.
IsoTherm system starter set, includes IsoSafe, IsoRack, 0 °C IsoPack and –21 °C IsoPack
For 0.5 ml tubes
For 1.5/2.0 ml tubes
022510002
022510053
IsoPack and IsoRack set, includes 1 each IsoPack and IsoRack
For 0.5 ml tubes, 0 °C
For 0.5 ml tubes, –21 °C
For 1.5 ml/2.0 ml tubes, 0 °C
For 1.5 ml/2.0 ml tubes, –21 °C
022510282
022510291
022510240
022510258
IsoRack, set of 4 racks
For 0.5 ml tubes
For 1.5/2.0 ml tubes
022510100
022510169
IsoSafe and IsoPack, includes one IsoSafe and three IsoPacks
For 0.5 ml tubes, 0 °C
For 0.5 ml tubes, –21 °C
For 1.5/2.0 ml tubes, 0 °C
For 1.5/2.0 ml tubes, –21 °C
022510282
022510291
022510240
022510258
In the U.S.: Eppendorf North America 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715
email: [email protected] • www.eppendorf.com
Sample Preparation | Instruments | Consumables
‡ 2 IsoRacks
115
Rack systems
PCR-Cooler
Application
‡ For the protection, transport and storage of sensitive
samples, as well as for safely stopping reactions
Product features
‡ Reliably cools samples for
‡ Prohibits freezing of samples
more than an hour at 0 °C*
due to special cone contour
‡ Temperature safety is
‡ The temperature of each
indicated by color change
individual well is indicated
when temperature is
by its color
exceeded: violet or dark blue
‡ For 96-well format
when ice cold, pink or light
‡ For 0.2 ml and 0.5 ml
blue above 7 °C
reaction tubes/PCR tubes,
‡ Space saving
tube strips and PCR plates 96
‡ Superior to an ice bath:
safe from contamination
*With two-hour precooling at –20 ˚C.
Description
By changing its color from violet to pink or dark blue to light blue,
the PCR-Cooler indicates when the temperature ceiling of 7 °C has
Instruments | Sample Preparation
been exceeded.
Temperature [°C]
Óx
Óä
ÓÓo
Inserted sample
£x
£ä
x
(0.1 ml volume)
Ço
ä
qx
Cooling aggregate
q£ä
(PCR-Cooler)
q£x
qÓä
q£ä
ä
£ä
Èä
£Óä
Time [min]
‡ Cooling curve
Ordering information
Temperature behavior of a precooled PCR-Cooler and a sample
Description
Catalog No.
PCR-Cooler
Starter set (1 pink, 1 blue)
Pink
Blue
022510509
022510541
022510525
inserted at room temperature (the background color indicates the
color change)
116
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.