BLOOD SAMPLE COLLECTION & HANDLING INSTRUCTIONS DRAWING THE BLOOD SAMPLE AT THE RIGHT TIME IS CRITICAL. The recommended draw volume is 3- 4 mL of blood to generate sufficient plasma for processing the test. If the drawn tube is not full, a redraw can be performed provided it occurs within the specified collection window. WARNING: For accurate MyImatinib test results, the blood sample must be taken by venous draw. FOR THE HEALTHCARE PROFESSIONAL CRITICAL BLOOD DRAW INFORMATION 116 Research Dr., Bethlehem, PA 18015 • Telephone: 855.419.6731 ©2014 Saladax Biomedical, Inc. All rights reserved. LIT-IM-002 Rev. 01 M M BLOOD SAMPLE COLLECTION & HANDLING INSTRUCTIONS STEP 2 BEGIN DOCUMENTATION STEP 4 CENTRIFUGE SAMPLE Centrifuge 3 - 4 mL of whole blood sample for a minimum of 10 minutes to separate the plasma from cells. 1. Record test name, ordering institution and requester information. Ideal: Centrifuge sample directly after completing the blood draw to separate plasma. 2. Record patient, cancer type, drug information, billing and authorization information. Alternative: Immediately place whole blood sample tube on wet ice or refrigerate at 2°- 8°C, then centrifuge sample within 1 hour of the draw. 3. Record treatment timing details. NOTE: Samples that are not centrifuged as per these instructions may cause incorrect results. FOR TECHNICAL OR CUSTOMER SUPPORT PLEASE CALL 855.419.6731 (PRESS 1) ORDER FORMS & TIME TABLE CHARTS AVAILABLE AT WWW.MYCARETESTS.COM STEP 1 FREEZE THE TRANSPORT COLD PACK > – 24 HOURS BEFORE USE NOTE: If transport cold pack is not frozen before time of blood draw, complete steps 1 to 5, then freeze and hold plasma at a temperature equal to or colder than - 20º C until transport cold pack is frozen. STEP 3 COLLECT SAMPLE BY VENOUS DRAW Collect 3 - 4 mL of blood into supplied K2EDTA tube. Draw sample after at least 29 days of imatinib therapy. On the day of collection, draw the sample before the next dose is taken. For once-daily dosing: Draw sample at least 21 hours from the last dose but before the next dose. For twice-daily dosing: Draw sample at least 9 hours from the last dose but before the next dose. DO NOT collect sample if patient imatinib dosing was interrupted. DO NOT do a short draw; fill tubes until vacuum stops drawing blood. DO NOT use plasma separator tubes. STEP 5 CAREFULLY DRAW OFF PLASMA from the top of the tube using the provided transfer pipette, avoiding the cell layer, and transfer to the cryotube. 1. Plasma must be free of cells. 2. Store plasma in the capped cryotube at 2°- 8°C prior to shipment to Saladax. 3. Freeze plasma at a temperature equal to or colder than - 20º C for shipment greater than 1 week after collection of the sample. 4. Complete any remaining documentation.