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YMC GENERAL CATALOG
9
Sample Pretreatment,
Accessories
Sample pretreatment
Column protection
166
167,168
Tubing parts
169∼172
Empty columns and other parts
173∼176
Pressure resistant empty glass
columns
177,178
165
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Sample pretreatment, Accessories
Material properties of products
S t a i n l e s s（SUS-316）
Polyacetal
A general-purpose material for general HPLC products.
An engineering plastic. A polymer in which aldehyde
Contains Ni, Cr, Mo, etc, as well as Fe, and is highly resistant
compounds are polymerized, with excellent chemical
to corrosion.
resistance and excellent machinability.
P E E K（polyetheretherketone）
Polypropylene
A super engineering plastic developed as an alternative to
A polymer of propylene. Excellent chemical and heat
metals.
resistance. Often used for containers since processing,
A polymer containing ketones and ether bonds, it is highly
such as injection molding, is easy.
resistant to pressure, chemicals, heat, and fatigue. Used as
an alternative to stainless for a metal-free tubing system.
T e f l o n（polytetrafluoroethylene, PTFE）
A polymer of tetrafluoroethylene. Highly resistant to
chemicals but not so resistant to pressure as PEEK.
Generally used for low pressure tubing systems.
(Teflon is a trademark of DuPont.)
1 Sample pretreatment
Sample pretreatment cartridge
Method of use
Syringe
C
YM
Adapter
Vacuum
YMC Dispo SPE
Excellent recovery
Sample
YMC Dispo Cartridge, Cartridge,
SPE
Company A Company B
Basic sample (Propranolol hydrochloride) 89
（99）
％ 59
（73）
％
Acidic sample (Thioctic acid)
91
（100）
％ 92
（89）
％
66
（89）
％
87
（88）
％
＊Figures inside parentheses represent recoveries obtained when buffer was used.
Container
Made from medical-grade polypropylene
with minimal elution of additives.
Upper and lower frits
Pore size of 20 µm
Luer tip
Can be connected to commercially
available vacuum solid phase
extraction systems.
PE
oS
Dis
p
●Disposable type cartridge made from polypropylene
●Utilizes packing material designed for HPLC columns,
manufactured under strict quality controls
●Excellent cartridge-to-cartridge reproducibility
●High recovery of samples
YMC Dispo SPE
Sample Pretreatment, Accessories
9
YMC Dispo SPE
Solid phase
YMC＊GEL
Vacuum method
Compression method
Centrifugation
●Vacuum method is suitable for large-scale treatment and compression
method is suitable for small-scale treatment
YMC Dispo SPE C18
Product No.
Specification
XQSPEC18101
XQSPEC18203
XQSPEC18503
XQSPEC18A06
C18 100mg/1mL 100-pack
C18 200mg/3mL 50-pack
C18 500mg/3mL 50-pack
C18 1000mg/6mL 30-pack
YMC Dispo SPE SIL
Product No.
Specification
XQSPESIL503
XQSPESILA06
SIL 500mg/3mL 50-pack
SIL 1000mg/6mL 30-pack
166
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2 Column protection
Stainless steel precolumn filters are convenient for the case shown below:
Problem prevention
One of the causes of a gradual increase of a column pressure after each
sample injection is that fine particles in the sample clog the frit of the
column. Replacing the column frit is one solution; however it is more
convenient to use precolumn filter. Connect a precolumn filter between
the injector and the column, check the line pressure occasionally, and
replace the precolumn filter when the line pressure increases.
Stainless steel precolumn filters are suitable for stainless steel tubing
systems. Both PEEK and stainless steel precolumn filters are suitable for
PEEK tubing systems.
High-molecular weight compounds (proteins, DNA, sugars,
etc.) that easily and irreversibly adsorb to the main column
are likely to cause a pressure increase and separation
failure. When these compounds adsorb to the main column,
it is difficult to recover column performance by cleaning.
A guard column will protect the main column when fouling
of the main column is due to contamination from sample
matrices.
YMC-Pack
YMC guard cartridges
Precolumn ﬁlter
Stainless steel precolumn filter
●Filter construction with minimal dead volume
Effective diameter of the filter is extremely small about 1.6
mm. Sample diffusion that occurs in passage is minimized.
Stainless steel precolumn filter
Product No.
XRPRCS02
XRPRCS25
Filter
Retainer
Specification
For tubing system with 1/16”outer diameter, 1 filter included
5 spare filters 2 µm are included
There is no decrease of column efficiency due to connection of a precolumn filter.
Main column only
3
1
4
2
3
4
Column
Theoretical plate number（peak 4）：11,200±200
（n=3）
Pressure
（MPa）：5.5±0
（n=3）
0
10
20 min
Theoretical plate number（peak 4）：11,500±300
（n=3）
Pressure
（MPa）：5.5±0
（n=3）
0
10
20 min
：YMC-Pack ODS-A (5 µm, 120 Å)
150×4.6 mmI.D.
Eluent
：acetonitrile/water (60/40)
Flow rate ：1.0 mL/min
Temperature：37 ℃
Detection ：UV at 270 nm, 0.32 AUFS
Sample
：1. Uracil (0.05 mg/mL)
2. Methyl benzoate (0.5 µL/mL)
3. Butyl benzoate (1.5 µL/mL)
4. Butylbenzene (12.0 µL/mL)
PEEK precolumn filter
●Metal free
Sample Pretreatment, Accessories
After connection of
a precolumn ﬁlter
1 2
9
The material of the wetted part of the holder is PEEK and the packing is PCTFE.
The packaging that of the filter is a mixture of PEEK and Teflon. PEEK precolumn
filters are suitable for use in HPLC systems requiring a metal-free environment.
●High-pressure resistance
PEEK precolumn filters are resistant to ca. 35 MPa.
Filter
PEEK precolumn filter
Product No.
XRPRCP02
XRPRCP25
Specification
For tubing systems with 1/16”outer diameter, 1 filter included
5 spare filters 2 µm are included
167
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Sample pretreatment, Accessories
2 Column protection
YMC guard cartridges
●Wide variety of packing materials
YMC＊GEL with various functional groups identical to
those available for analytical columns is packed under high
pressure.
●Disposable
The cartridge holder can be used repeatedly. When the YMC
guard cartridge needs replacement must be replaced. Only
the cartridge column.
cartridge column
●Easy cartridge replacement
cartridge holder
The main body of the YMC guard cartridge is composed of
a cartridge holder and a cartridge column. The cartridge
column is threaded for easy replacement.
Main column only
After connection of
YMC guard cartridge
After connection of Company
A’
s guard cartridge
3
3
12
1
2
This figure shows chromatograms
produced using a YMC-Pack ODSAM(150×4.6 mm I.D.) not-connected
to a guard cartridge (Left), while
connected a YMC guard cartridge
(Center), and while connected to
Company A’
s guard cartridge (Right).
O
1
2
1. Uracil
HN
O
N
H
3
2. Acetylacetone
O
O
3. 8-Hydroxy quinoline
N
OH
Sample Pretreatment, Accessories
9
Eluent
0
5
10
0
min
5
10
min
0
5
10
min
（When a different packing material from that of the main column is used, peak shapes may be affected.）
YMC guard cartridges
Semi-micro guard cartridges
Guard cartridge holders
Product No.
XPCHW
Semi-micro guard cartridge holder（commonly used for inner diameters of 1.0, 1.5, and 2.0 mm）
Specification
For a cartridge column
Cartridge columns（3-pack）
Product No.
AS12S05-G304CC
AA12S05-G304CC
AM12S05-G304CC
AQ12S05-G304CC
For
For
For
For
：20 mM K2HPO4-KH2PO4
(pH 7.6)
/methanol (40/60)
Flow rate ：1.0 mL/min
Temperature：37 ℃
Detection ：UV at 254 nm, 0.32 AUFS
Injection
：5 µL (70 ～ 250 µg/mL)
Specification
YMC-Pack Pro C18
YMC-Pack ODS-A
YMC-Pack ODS-AM
YMC-Pack ODS-AQ
●Suitable for analytical columns with 4.0 to 6.0 mm inner diameters.
！
△For other functional groups, see pages for each analytical column.
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Product No.
XPCHSMW
Specification
For a semi-micro cartridge column
Semi-micro guard cartridge for columns with 2.0 mm inner diameter（2-pack）
Product No.
AS12S05-0102CC
AA12S05-0102CC
AM12S05-0102CC
AQ12S05-0102CC
Specification
For YMC-Pack Pro C18
For YMC-Pack ODS-A
For YMC-Pack ODS-AM
For YMC-Pack ODS-AQ
●Suitable for analytical columns with inner diameters of 2.0 to 3.0 mm.
！
△For columns with inner diameters of 1.0 mm and 1.5 mm, see pages for each
analytical column.
A semi-micro guard cartridge holder will need to be purchased separately before
using this product for the first time.
168
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3 Tubing parts
Universal connector
●Enables perfect column connectivity
A movable ferrule and a fixing screw enable secure
connection to the column endfitting. There is no cavity at the
top of the ferrule.
Double type
●Super high-pressure resistance
Universal connectors are high performance connectors
resistant to high pressure up to 70 MPa when tightened only
by hand.
Single type
●For connection to a guard column
The double type is the most suitable for connecting of a main
column and a guard column.
rs
panne
s
r
o
f
e.
ed
No ne and reliabl
sy
ea
Fixing
screw
Fixing
ferrule
Adjuster
screw
Sealing
ferrule
（replacable）
The tube makes tight contact
with the screw hole by
tightening a ﬁxing screw.
Universal connector（single）
Product No.
XRSFS520
XRSFS220
Specification
1/16”outer diameter,0.005”inner diameter, 20cm length
1/16”outer diameter,0.020”inner diameter, 20cm length
Universal connector（double）
Product No.
XRSFD506
XRSFD510
XRSFD520
XRSFD220
Specification
1/16”outer diameter,0.005”inner diameter, 6cm length
1/16”outer diameter,0.005”inner diameter, 10cm length
1/16”outer diameter,0.005”inner diameter, 20cm length
1/16”outer diameter,0.020”inner diameter, 20cm length
Universal connector（Single flexible）
Product No.
XRSFS528F
XRSFS540F
Specification
1/32”outer diameter,0.005”inner diameter, 28cm length
1/32”outer diameter,0.005”inner diameter, 40cm length
Sample Pretreatment, Accessories
●Tightening the ﬁxing screw forces a tube to squeeze
into the column as far as it will go, and the tube makes
tight contact with the screw hole.
Everyone can connect columns easily and securely.
9
●outer diamater of metted part is 1/16"
Spare ferrule for universal connectors
Product No.
XRSFV
Specification
Vespel sealing ferrule, 5-pack
169
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Sample pretreatment, Accessories
3 Tubing parts
Handy connector 1
●A ferrule integrated into a connector
Handy connector 1 resists pressure to ca. 21 MPa. Handy connector 1 is suitable
for tubing systems of relatively low pressure. Connection to a tubing system is
easy because a ferrule is integrated into the connector.
●Metal free
The whole connector is made from PEEK. Handy connector 1 is suitable
for metal-free tubing systems using PEEK tubes.
Handy connector 1
Product No.
XRP0203
PEEK
Specification
For tubing systems of 1/16”outer diameter, 10-pack
Handy connector 2
●High pressure resistance and made from stainless steel
Handy connector 2 resists pressure to ca. 42 MPa. Handy connector 2 is suitable
for connection to the injection side of a column, to which high pressure is applied.
It is also useful for connection of a guard column or a precolumn filter.
●Metal free
The ferrule at the wetted part of the connector is made from PEEK. Handy
connector 2 is suitable for metal-free tubing systems using PEEK tubes.
●Ferrule is replaceable
Only the ferrule needs to be replaced when it deteriorates, as opposed to
the entire connector.
Stainless steel
Ferrule for replacement
Handy connector 2
Product No.
XRP0204
XRP0205
Specification
For tubing system of 1/16”outer diameter, 2-pack
Spare PEEK ferrule, 10-pack
●Be aware that Handy connector 2 cannot be connected to“Old Shimadzu type”
,“Old
JASCO type”
, or“Old Hitachi type”tubes.
Sample Pretreatment, Accessories
9
nners
a
p
s
r
d fo
No nee and reliable.
y
eas
（1）
Extend the top of PEEK tube
beyond the ferrule.
（3）
Turn the connector while pushing
the tube to connect the tube and
connector.
（4）
Be sure that there is no leak from
the screw hole.
（2）
Force the tube to make tight contact
with the bottom of the screw hole.
170
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PEEK tubing
Outer diameter1/16”
●Easy to use
PEEK tubing is made from a material that is flexible and easy to use.
It is easily cut using a commercially available cutting knife. A PEEK
tube with an inner diameter of 0.127 mm is useable at up to 40 MPa.
PEEK tubes are practical general tubing as alternatives to stainless
steel tubing. They are suitable for metal-free tubing systems. A Handy
connector is suitable for connection of PEEK tubing to a column.
Stainless steel tubing
●Stainless steel male nut and ferrule are used for connection
Inner diameter
0.127mm
0.25mm
Outer diameter1/16”
0.5mm
0.8mm
Outer diameter1/8”
Tube cutter
●For cutting 1/16”stainless tubing（simple type）
Inner diameter
0.25mm
0.5mm
0.8mm
2.0mm
Male nut for 1/16″tubing system（stainless steel）
W type P type
Old Shimadzu type
Old JASCO type
Female nut for 1/8″
tubing system
Old Hitachi type
Ferrule for 1/8″tubing system
（stainless steel）
Male nut for 1/16″
tubing system
Ferrule for 1/16″tubing system
Female nut for 1/8″tubing system
（cap nut）（stainless steel）
Union for 1/16″tubing system
Unions for 1/16”tubing system come in two
types, made from either stainless steel or PEEK.
They are convenient to use it with a Handy
connector. Connector type is“W type”.
Column coupler
Column couplers are used for columnto-column connections. Various tube
inner diameters and column connection
types are available.
Sample Pretreatment, Accessories
Ferrule for 1/16″tubing system
9
Ferrule for 1/8″tubing system
（stainless steel, double ring）
Stainless steel
PEEK
Cross section
171
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Sample pretreatment, Accessories
3 Tubing parts
PEEK tubing
Product No.
XRPT01
XRPT02
XRPT05
XRPT08
n
nio
r1
fo
b
u
”t
6
/1
ing
Stainless steel tubing
em
st
sy
Specification
1/16”outer diameter, 0.127 mm inner diameter, 3m length
1/16”outer diameter, 0.25 mm inner diameter, 3m length
1/16”outer diameter, 0.5 mm inner diameter, 3m length
1/16”outer diameter, 0.8 mm inner diameter, 3m length
Product No.
XRST02
XRST05
XRST08
XRST20
U
Specification
1/16”outer diameter, 0.25 mm inner diameter, 2m length
1/16”outer diameter, 0.5 mm inner diameter, 2m length
1/16”outer diameter, 0.8 mm inner diameter, 2m length
1/8” outer diameter, 2.0 mm inner diameter, 5m length
Tube cutter
Product No.
XRP1909
Specification
For cutting 1/16”stainless tubing
Male nut for 1/16”tubing system（stainless steel）
Notes for successful tubing
The shapes of separation peaks are affected not only by column
performance, but by the tubing system as well.
Some effort is needed to minimize the dead volume in a tubing
system among the injector column and detector, in order to prevent
poor peak shape. Using a tube with a small inner diameter (0.127
mm) is one solution. When a tube is connected to a union or a
column, it is important to give due consideration to make the top of
the tube contact flush with the bottom of the screw hole and to avoid
leakage or creation of a cavity.
Product No.
XRONW
XRONS
XRONJ
XRONH
Specification
1/16”W type, P type, 5-pack
1/16”Old Shimadzu type, 5-pack
1/16”Old JASCO type, 5-pack
1/16”Old Hitachi type, 5-pack
Ferrule for 1/16”tubing system（stainless steel）
Product No.
XRONFA
Specification
1/16”one ring ferrule, 5-pack
Female nut for 1/8”tubing system（cap nut）（stainless steel）
Product No.
XRON8
Specification
1/8”female nut, 5-pack
Ferrule for 1/8”tubing system（stainless steel）
Product No.
XRTWFA
Sample Pretreatment, Accessories
9
Specification
Front ferrule, back ferrule, 5-pack each
Union for 1/16”tubing system
es
idg
rtr
a
8）
c
d
13
ar
e
u
g
g
pa
（
r
lum
n
Co
co
le
up
Product No.
XRUNW
XRUN16P
Specification
1/16”tubing system（stainless steel）
1/16”tubing system（PEEK）
Column coupler
Product No.
XRCP1602
XRCP1605
XRCP1620
Specification
1/16”outer diameter, 0.25 mm inner diameter
1/16”outer diameter, 0.5 mm inner diameter
1/16”outer diameter, 0.8 mm inner diameter
（Stainless tube 50 mm×1, male nut×2, ferrule×2）
YM
ck
Pa
C-
172
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4 Empty columns and other parts
1 YMC original empty columns
Analytical
column
Inner diameter
2.0∼10mm
2 Ferrule type empty column
3 Flange type empty column
Semi-preparative
column
Inner diameter
20∼30mm
Inner diameter
4.0∼6.0mm
Inner diameter
50∼200mm
YMC original empty columns（for analysis or semi-preparative isolation）
A stainless steel tube with a screw and an endfitting are directly connected (screw type). Since YMC original empty columns use no
ferrule, there is no danger of endfitting dislocation when the column is packed and used under high pressure.
YMC original empty columns
Product No.
50
150
250
30
50
100
150
250
300
50
100
150
250
300
500
30
300
500
30
150
250
300
500
2.0
4.6
6.0
8.0
Product No.
Column size
length×inner diameter（mm）
XC0520WT
XC1520WT
XC2520WT
XC3020WT
XC5020WT
XCA020WT
XCL530WT
XC2530WT
XC3030WT
XC5030WT
XCA030WT
50
150
250
300
500
1000
75
250
300
500
1000
20.0
30.0
Sample Pretreatment, Accessories
XC0502WT
XC1502WT
XC2502WT
XC0346WT
XC0546WT
XC1046WT
XC1546WT
XC2546WT
XC3046WT
XC0506WT
XC1006WT
XC1506WT
XC2506WT
XC3006WT
XC5006WT
XC0308WT
XC3008WT
XC5008WT
XC0310WT
XC1510WT
XC2510WT
XC3010WT
XC5010WT
Column size
length×inner diameter（mm）
9
10.0
173
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Sample pretreatment, Accessories
4 Empty columns and other parts
YMC original empty column parts
Inner diameter 2.0mm、4.6mm、6mm、8mm、10mm
Column end
assembly
Frit
Inner diameter 20mm、30mm
Column end assembly
Sample Pretreatment, Accessories
9
Spacer
Distributor
Frit
YMC original empty column parts
Product No.
XPEF02
XPEF46
XPEF06
XPEF08
XPEF10
XPEF20
XPEF30
XPSF02S
XPSF46S
XPSF06S
XPSF08S
XPSF10S
XPSF20S
XPSF30S
XPSS20S
XPSS30S
Product name
For 2.0 mm inner diameter, Column end assembly,1-pack
For 4.6 mm inner diameter, Column end assembly,1-pack
For 6.0 mm inner diameter, Column end assembly,1-pack
For 8.0 mm inner diameter, Column end assembly,1-pack
For 10 mm inner diameter, Column end assembly,1-pack
For 20 mm inner diameter, Column end assembly,1-pack
For 30 mm inner diameter, Column end assembly,1-pack
For 2.0 mm inner diameter, Frit,
5-pack
For 4.6 mm inner diameter, Frit,
5-pack
For 6.0 mm inner diameter, Frit,
5-pack
For 8.0 mm inner diameter, Frit,
5-pack
For 10 mm. inner diameter, Frit,
5-pack
For 20 mm. inner diameter, Frit,
2-pack
For 30 mm. inner diameter, Frit,
2-pack
For 20 mm. inner diameter, Spacer,
1-pack
For 30 mm. inner diameter, Spacer,
1-pack
Main column part names
Frit
A disk type part used to keep the packing material in a column.
A frit with a pore size of 2 µm is generally used for 5 µm packing
material.
Distributor
A part used to uniformly introduce a sample in mobile phase to
a column. Its structure enables uniform diffusion of the sample
over the frit surface.
●Frits with 20 mm or more inner diameter include distributors.
！
△Connector type is“W type”.
174
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Ferrule type empty column
Ferrule type empty columns are standard-type empty columns. A stainless steel tube and an endfitting are tightly connected using a ferrule.
Ferrule type empty column
Ferrule type empty column parts
Product No.
XPEF46F
XPEF04F
XPEF05F
XPSF46F
XPSF04F
XPSF05F
Column size
length×inner diameter（mm）
Product No.
10
10
50
100
150
250
XC0104WF
XC0105WF
XC0546WF
XC1046WF
XC1546WF
XC2546WF
4.0
5.0
4.6
Product name
For 4.6 mm inner diameter, Column end assembly,1-pack
For 4.0 mm inner diameter, Column end assembly,1-pack
For 5.0 mm inner diameter, Column end assembly,1-pack
For 4.6 mm inner diameter, Frit,
5-pack
For 4.0 mm inner diameter, Frit,
5-pack
For 5.0 mm inner diameter, Frit,
5-pack
Column end
assembly
Frit
Flange type empty column（for preparative isolation）
A stainless steel tube with a flange and a cap are tightly connected using a bolt. The inner diameter is 50 mm or more.
Flange type empty column
50mm inner diameter
70mm inner diameter
Column size
Max pressure
length×inner diameter（mm）（MPa）
XC0551■R
XC1051■R
XC2551■R
XC3051■R
XC5051■R
XCA051■R
XC0552■R
XC1052■R
XC2552■R
XC3052■R
XC5052■R
XCA052■R
50
100
250
300
500
1000
50
100
250
300
500
1000
50.0
50.0
7
14
！
△D esignate a frit pore size depending on particle size.
We recommend 2
µm for particle size below 10 µm,
5 µm for particle size above 15 µm.
※■
＝Connection type: Please select your
desired connection type.
A：1/16" male nut
D：1/8" female nut
E：1/4" female nut
Product No.
Column size
Max pressure
length×inner diameter（mm）（MPa）
XC1071■R
XC2571■R
XC3071■R
XC5071■R
XCA071■R
XC1072■R
XC2572■R
XC3072■R
XC5072■R
XCA072■R
100
250
300
500
1000
100
250
300
500
1000
70.0
70.0
7
14
Product No.
Column size
Max pressure
length×inner diameter（mm）（MPa）
XC20B1■R
XC30B1■R
XC50B1■R
XCA0B1■R
XC20B2■R
XC30B2■R
XC50B2■R
XCA0B2■R
200
300
500
1000
200
300
500
1000
150.0
7
150.0
14
！
△D esignate a frit pore size depending on particle size.
We recommend 2
µm for particle size below 10 µm,
5 µm for particle size above 15 µm.
100mm inner diameter
200mm inner diameter
Product No.
Column size
Max pressure
length×inner diameter（mm）（MPa）
XC10A1■R
XC30A1■R
XC50A1■R
XCA0A1■R
XC10A2■R
XC30A2■R
XC50A2■R
XCA0A2■R
100
300
500
1000
100
300
500
1000
100.0
7
100.0
14
Product No.
Column size
Max pressure
length×inner diameter（mm）（MPa）
XC20C1■R
XC50C1■R
XCA0C1■R
XC20C2■R
XC50C2■R
XCA0C2■R
200
500
1000
200
500
1000
200.0
7
200.0
14
Sample Pretreatment, Accessories
Product No.
150mm inner diameter
9
175
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Sample pretreatment, Accessories
4 Empty columns and other parts
Flange type empty column parts
Product No.
Product name
Max pressure
（MPa）
XPCP052
XPCP053
XPSF0522
XPSF0532
XPSF0525
XPSF0535
XPRI052
XPRI053
XPBL052
XPBL053
XPSU162
XPSU163
XPSU82
XPSU83
For inner diameter 50 mm, Cap
For inner diameter 50 mm, Cap
For inner diameter 50 mm, 2 µm frit（with distributor）
For inner diameter 50 mm, 2 µm frit（with distributor）
For inner diameter 50 mm, 5 µm frit（with distributor）
For inner diameter 50 mm, 5 µm frit（with distributor）
For inner diameter 50 mm, Stainless SS ring
For inner diameter 50 mm, Stainless SS ring
For inner diameter 50 mm, bolt set（8 pairs）
For inner diameter 50 mm, bolt set（12 pairs）
For inner diameter 50 mm, 1/16”male nut for connection adaptor
For inner diameter 50 mm, 1/16”male nut for connection adaptor
For inner diameter 50 mm, 1/08”female nut for connection adaptor
For inner diameter 50 mm, 1/08”female nut for connection adaptor
7
14
7
14
7
14
7
14
7
14
7
14
7
14
Speciﬁcation of ﬂange type empty column
Connection adaptor
Distributor
Frit
Spacer
Cap
＊For column parts with an inner diameter of 70 mm or more, please contact us.
Bolt set
Airtight stopper
Sample Pretreatment, Accessories
9
Airtight stoppers are used to prevent column contents from drying.
Please use a stopper appropriate for each column connection type.
1/16" airtight stopper
Product No.
XPMSY
XPMSW
Specification
YMC original type（PEEK）
,
Waters type（stainless）
,
5-pack
5-pack
1/8" airtight stopper
Product No.
XPMS8
YMC original type
W type
Specification
Female nut,
2-pack
1/8" airtight stopper
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5 Pressure resistant empty glass columns
●Suitable for preparative chromatography
Pressure resistant empty glass columns are useful for early
purification of natural products, after-treatment of open column
chromatography and pre-treatment of preparative HPLC.
They are also suitable for removal of impure compounds produced
during organic synthesis.
●The most suitable for self-packing
Low-cost preparative isolation can be achieved by self-packing.
Pressure resistant empty glass columns are highly flexible tools for
preparative isolation because many types of packing materials may
be packed into the column.
●Pressure resistance
YMC empty glass columns, made from Pyrex glass, have excellent
pressure resistance. They are suitable for spherical and irregular
packing materials with a particle size of 50 µm or more.
●Applicable to medium-pressure and high-performance liquid
chromatography systems
Pressure resistant empty glass columns can be connected to
existing chromatographs, making the preparative isolation easy.
●Metal free column
No metal is used for wetted parts. There is no danger of corrosion
caused by acids or of metal contamination in isolation fractions.
Pressure resistant empty glass columns are suitable for a metal-free
HPLC system.
●Visibility
In contrast to stainless steel columns, the separation behavior of
samples analyzed by chromatography can be observed visually. It is
useful for preparative isolation or removal of colored material.
Dry-packed with S-50 gel（silica）
Dry-packed with S-50 gel（ODS）
2
A
B
2
1
1
N=2,700
1
N=2,200
N=2,500
3
These figures show typical
measurement results for
theoretical plate numbers
obtained using two YMC
pressure resistant glass
columns, XC5020FG; one was
dry-packed with C18 and the
other with silica gel (S-50 gel,
spherical type, particle size of
50 µm).
For this column size, the
volume of packing material
used per column is ca. 80 g.
4
INJ.
0
Flow Rate
Pressure
INJ.
INJ.
5
10
15
min
0
5
10 mL/min
0.2 MPa
Packing
：YMC＊GEL SIL, S-50 µm, 120 Å
Column Size：500×20 mmI.D.
Eluent
：n-hexane/ethanol (90/10)
Detection ：UV at 254 nm, 0.16 AUFS
Sample
：1. Toluene　2. Nitrobenzene
Injection
：20 µL
10
min
0
10 20 30 40
Sample Pretreatment, Accessories
2
9
min
20 mL/min
0.3 MPa
Packing
：YMC＊GEL ODS-A, S-50 µm, 120 Å
Column Size：500×20 mmI.D.
Eluent
：methanol/water (75/25)
Flow Rate ：10 mL/min
Pressure
：0.3 MPa
Detection ：UV at 254 nm, 0.32 AUFS
Sample
：1. Uracil　2. Methyl benzoate　3. Toluene　4. Naphthalene
Injection
：20 µL
177
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Sample pretreatment, Accessories
5 Pressure resistant empty glass columns
Pressure resistant empty glass columns（glass tube, column endfitting, tubing fitting kit, airtight stopper）
Product No.
Inner diameter
8mm
20mm
30mm
Length
100mm
XC1008FG
XC1020FG
XC1030FG
300mm
XC3008FG
XC3020FG
XC3030FG
500mm
XC5008FG
XC5020FG
XC5030FG
Parts
①Glass tube
Product No.
Inner diameter
8mm
20mm
30mm
Length
100mm
XT1008FG
XT1020FG
XT1030FG
300mm
XT3008FG
XT3020FG
XT3030FG
②Column endfitting（includes frit and packing）
Product No.
XPEA8
XPEA20
XPEA30
500mm
XT5008FG
XT5020FG
XT5030FG
③Glass frit（with packing）
Specification
For inner diameter 8 mm, 1-pack
For inner diameter 20 mm, 1-pack
For inner diameter 30 mm, 1-pack
Product No.
XPFP83
XPFP2010
XPFP3010
Specification
For inner diameter 8 mm, 3 µm pore size
For inner diameter 20 mm, 10 µm pore size
For inner diameter 30 mm, 10 µm pore size
One column requires 2 column endfittings.
④Tubing fitting kit（ferrule, o-ring, male nut）
Product No.
XRFK001
Fitting kit, a set of 2 kits, IN-side and OUT-side, for 1/16”O.D. Teflon tube or
stainless tube.
Sample Pretreatment, Accessories
9
⑤Special-purpose Teflon tube
Specification
For inner diameter 8, 20,30 mm（common use）
Product No.
XRTT102
XRTT105
Specification
1/16”outer diameter, 0.25 mm inner diameter, 10 m
1/16”outer diameter, 0.5 mm inner diameter, 10 m
For tubing fitting kits
Column connection joint（column union and flat seal（2-pack））
①Glass tube
Product No.
XRCJ8
XRCJ20
XRCJ30
Specification
For inner diameter 8 mm
For inner diameter 20 mm
For inner diameter 30 mm
Used for connection of columns with same diameter in packing.
For large columns not described above, please contact us.
5
1
4
3
2
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