1. health and fitness
2. disease
3. cholesterol

Products for Protein Sample Preparation
protea
Protein Sample Preparation
Protein
Sample
Preparation
ProteaPrep Albumin Depletion Sample Prep Kit
One of the major problems faced in the analysis of the human
serum proteome is the broad dynamic range of its protein
constituents. High abundance proteins, such as human serum
albumin (HSA) and immunoglobulin gamma (IgG), which together
comprise ~75% of the total serum protein content, inhibit the
analysis of lower abundance proteins of interest.
Affinity-based depletion strategies are often employed to
selectively remove high abundance proteins, such as HSA and
IgG, to concentrate the lower abundance proteins and facilitate
their analysis. Traditionally, antibody-based schemes are used
to produce affinity-binding ligands for HSA and IgG, where
monoclonal or polyclonal antibodies are produced in a source
animal, purified, and then bound to a surface (e.g. bead or well)
for preparation of serum samples. Variability in the production
of these antibodies leads to a wide variety of specificities and
selectivities, as the binding of the ligand to the HSA or IgG is
dependent upon the availability of the epitope, the binding affinity
of the antibody, and the homogeneity of the antibody population.
Thus, the efficiencies for antibody-based depletion strategies
for the selective removal of high abundance proteins in human
serum can vary broadly, from 70 to 95%, and can dramatically
affect the reproducibility of studies of the human serum proteome.
The ProteaPrep Albumin Depletion Sample Prep Kit (SP-200)
features a revolutionary new approach to affinity-based serum
albumin removal that utilizes a non-antibody-based method. The
ProteaPrep Albumin Depletion capture ligand is a recombinant
protein that exhibits excellent batch-to-batch reproducibility and
is highly purified for robust, efficient removal of albumin from
human serum samples. This proprietary ligand design technology
produces binding constants with human serum albumin
(Kd ~1 x 10 -11 M) that are significantly stronger than antibodybased methods, which have binding constants that range
from Kd’s of 1 x 10 -6 to 1 x 10 -8 M. The net result is that the
functionalized beads irreversibly bind and remove the albumin
from serum in a highly efficient and rapid manner. Depletion
efficiencies are >99% for the selective removal of HSA from
serum samples in less than 20 minutes. The ProteaPrep Albumin
Depletion Sample Prep Kit is also effective for the removal of
human, rat, mouse, bovine, pig, goat, horse, rabbit, monkey and
dog serum albumin.
FEATURES:
•
•
•
•
•
>99% efficiency for depletion of human serum albumin
Simple and easy to use – process samples in less than 20
minutes
New recombinant affinity ligand permits highly specific and
reproducible albumin removal to facilitate analysis of lower
abundance serum proteins
Albumin binding affinity 2 to 3 orders of magnitude greater
than traditional antibody-based albumin depletion methods
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
RESEARCH APPLICATIONS:
•
•
•
Human serum proteome analysis
Depletion of albumin from serum, plasma, and cell culture
media
Preparation of serum samples for analysis by SDS-PAGE,
2D gel electrophoresis, and mass spectrometry
www.proteabio.com • 2
Coomassie blue stained SDS-PAGE gel demonstrating the
effectiveness of the SP-200 ProteaPrep Albumin Depletion
Sample Prep kit for the removal of human serum albumin
(HSA) from human serum samples.
Key: Lane 1 = molecular weight markers; Lanes 2 and 4 =
1.0 µL of two unique human serum control samples with the
prominent HSA bands marked with an arrow; Lanes 3 and 5 =
albumin-depleted 1.0 µL samples showing the >99% removal
of HSA from the respective human serum samples.
ORDERING INFORMATION:
ProteaPrep Albumin Depletion Sample Prep Kit
Contains SpinTubes with functionalized beads and Bead
Activation Buffer
Catalog Number
Size
Price
SP-200-12
12 SpinTubes
$385
SP-200-24
24 SpinTubes
$770
SP-200-4x24
4 x 24 SpinTubes
$3080
Protein Sample Preparation
ProteaPrep Albuminome Analysis Sample Prep Kit
The ProteaPrep Albuminome Analysis Sample Prep Kit (SP-205)
includes the ProteaPrep Albumin Depletion Sample Prep Kit
(SP-200) for efficient capture of human serum albumin (HSA) on
the recombinant protein ligand and a Trypsin Digestion Kit
(PE-151) for on-bead digestion of the HSA and any proteins that
are bound or associated with HSA (see table below).
FEATURES:
•
•
•
•
•
>99% efficiency for capture of human serum albumin
New recombinant affinity ligand permits highly specific and
reproducible albumin removal to facilitate analysis of lower
abundance serum proteins
Albumin binding affinity 2 to 3 orders of magnitude greater
than traditional antibody-based albumin depletion methods
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
Trypsin Digestion Kit included for on-bead digestion of
human serum albumin and associated proteins
ORDERING INFORMATION:
RESEARCH APPLICATIONS:
•
•
•
Human albuminome analysis
Human serum proteome analysis
Preparation of serum samples for analysis by SDS-PAGE,
2D gel electrophoresis, and mass spectrometry
ProteaPrep Albuminome Analysis Sample Prep Kit
Contains SpinTubes with functionalized beads, Bead Activation
Buffer, and a Trypsin Digestion Kit (PE-151)
Catalog Number
SP-205-12
SP-205-24
SP-205-4x24
Size
12 SpinTubes
24 SpinTubes
4 x 24 SpinTubes
Price
$495
$990
$3960
Summary of on-bead digestion of albuminome for protein identification with LC-MS/MS. 10 µL aliquots of human serum
were preped with the SP-205 kit. The proteins associated or complexed with the bound albumin were digested on-bead
and analyzed by LC-MS/MS with different injection volumes and gradient lengths.
Parameters
On-bead Digestions of Abuminome Analyzed by LC-MS/MS
Gradient Time
(min)
25
60
60
60
90
90
90
Volume Injected
(μL)
15
15
30
45
15
30
45
% Total Albuminome
Injected
4.2%
4.2%
8.4%
12.6%
4.2%
8.4%
12.6%
Total Proteins ID >95%
36
53
61
58
63
76
85
3 • 877.776.8321
ProteaPrep Albumin Capture Sample Prep Kit
Serum albumin comprises about one-half of the human serum
protein and functions primarily as a carrier protein for steroids,
fatty acids, thyroid hormones, and other plasma proteins.
Carrier proteins serve an important role for sequestering and
transporting a large variety of biomolecules and pharmaceuticals
throughout the body. The analysis of these bound molecules
can reveal information about the health of an organism and the
bioavailability of pharmaceutical agents.
Human serum albumin (HSA) is a monomeric protein with
a molecular weight of ~65,000 Da. While HSA maintains
an important albumin-bound peptide and protein complex
(ABPPC), it is also an important diagnostic biomarker itself. HSA
undergoes a number of normal enzymatic modifications, such as
glycosylation and oxidation, but it can also be modified by other
mechanisms that are indicative of the health of an organism.
Direct analysis of the intact structure of the HSA protein can
reveal information about the pathological changes in an injured or
diseased patient, and the albumin profile can be diagnostic of that
pathology.
The ProteaPrep Albumin Capture Sample Prep Kit (SP-210)
utilizes a proprietary technology that incorporates a reversible
binding recombinant affinity ligand for highly efficient capture
(>99%) and release (>80%) of human serum albumin from plasma
and serum samples in less than 30 minutes. The ProteaPrep
Albumin Capture Sample Prep Kit is effective for the capture and
release of human, rat, mouse, bovine, pig, goat, horse, rabbit,
monkey and dog albumin for downstream analysis of intact serum
albumin and its binding partners.
Coomassie blue stained SDS-PAGE gel showing the capture
and recovery of 400 µg human serum albumin (HSA) using
the SP-210 ProteaPrep Albumin Capture Sample Prep Kit.
Lanes 1-5 show recovery controls based on 90%, 80%, 70%,
60%, 50% of 400 µg of HSA, respectively. Lane 6 is blank.
Lane 7 shows the eluate from 400 µg of HSA captured and
recovered with 80% efficiency using the SP-210 ProteaPrep
Albumin Capture Sample Prep Kit.
FEATURES:
ORDERING INFORMATION:
•
Contains SpinTubes with functionalized beads, Bead Activation
Buffer, and Albumin Release Reagent
Catalog Number
Size
Price
SP-210-12
12 SpinTubes
$425
SP-210-24
24 SpinTubes
$850
SP-210-4x24
4 x 24 SpinTubes
$3400
•
•
•
•
>99% efficiency for capture of human serum albumin
>80% efficiency for recovery of human serum albumin
Simple and easy to use – process samples in less than 30
minutes
New recombinant affinity ligand permits highly specific
and reproducible albumin capture and recovery to facilitate
analysis albumin and albumin-bound peptides and proteins
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
RESEARCH APPLICATIONS:
•
•
•
Intact albumin analysis
Serum albuminomics
Preparation of serum samples for analysis by SDS-PAGE,
2D gel electrophoresis, and mass spectrometry
www.proteabio.com • 4
ProteaPrep Albumin Capture Sample Prep Kit
Protein Sample Preparation
ProteaPrep IgG Depletion Sample Prep Kit
Immunoglobulins comprise approximately 15 – 20% of the blood
serum protein, with immunoglobulin G (IgG) constituting up to
75% of the serum immunoglobulins in humans. IgG antibodies
are tetramers with an average molecular weight of 150 kDa,
and they are comprised of two identical heavy chains (~50 kDa
each) and two identical light chains (~25 kDa each). The two
heavy chains are linked to each other and to a light chain each
by disulfide bonds, and the resulting tetramer has two identical
halves which together form the Y-like characteristic shape of the
antibody.
Affinity-based depletion strategies are often employed to
selectively remove high abundance proteins, such as HSA and
IgG, to concentrate the lower abundance proteins and facilitate
their analysis. Traditionally, antibody-based schemes are used
to produce affinity-binding ligands for HSA and IgG, where
monoclonal or polyclonal antibodies are produced in a source
animal, purified, and then bound to a surface (e.g. bead or well)
for preparation of serum samples. Variability in the production
of these antibodies leads to a wide variety of specificities and
selectivities, as the binding of the ligand to the HSA or IgG is
dependent upon the availability of the epitope, the binding affinity
of the antibody, and the homogeneity of the antibody population.
Thus, the efficiencies for antibody-based depletion strategies for
the selective removal of high abundance proteins in human serum
can vary broadly, from 70 to 95%, and can dramatically affect the
reproducibility of studies of the human serum proteome.
The ProteaPrep IgG Depletion Sample Prep Kit (SP-220)
features a revolutionary new approach to affinity-based serum
immunoglobulin removal that utilizes a non-antibody-based
method. The ProteaPrep IgG Depletion capture ligand is a
recombinant protein that binds with high affinity to IgG, exhibits
excellent batch-to-batch reproducibility, and is highly purified for
robust, efficient removal of IgG from human serum samples. The
net result is that the functionalized beads irreversibly bind and
remove the IgG from serum in a highly efficient and rapid manner.
Depletion efficiencies are >99% for the selective removal of IgG
from serum samples in less than 20 minutes.
FEATURES:
•
•
•
•
•
>99% efficiency for depletion of human IgG
Simple and easy to use – process samples in less than 20
minutes
New recombinant affinity ligand permits highly specific
and reproducible IgG removal to facilitate analysis of lower
abundance serum proteins
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
Preparation of serum samples for analysis by SDS-PAGE
gel electrophoresisand mass spectrometry
Coomassie blue stained SDS-PAGE gel demonstrating >99%
depletion of IgG from 10 µL of human serum using the SP-220
ProteaPrep IgG Depletion Sample Prep Kit. The black arrow
indicates the IgG protein band in the control sample that is not
observed in the depleted sample.
Key: Lane 1 = molecular weight marker; Lane 2 = 10 µL human
serum control, 10% sample load; Lane 3 = 10 µL human serum,
IgG depleted, 10% sample load.
ORDERING INFORMATION:
ProteaPrep IgG Depletion Sample Prep Kit
Contains SpinTubes with functionalized beads and Bead
Activation Buffer
Catalog Number
SP-220-12
SP-220-24
SP-220-4x24
Size
12 SpinTubes
24 SpinTubes
4 x 24 SpinTubes
Price
$385
$770
$3080
RESEARCH APPLICATIONS:
•
•
•
Human serum proteome analysis
Depletion of IgG from serum and plasma samples
Preparation of serum samples for analysis by SDS-PAGE,
2D gel electrophoresis, and mass spectrometry
5 • 877.776.8321
ProteaPrep IgG Capture Sample Prep Kit
Immunoglobulins comprise approximately 15 – 20% of the blood
serum protein, with immunoglobulin G (IgG) constituting up to
75% of the serum immunoglobulins in humans. IgG antibodies
are tetramers with an average molecular weight of 150 kDa,
and they are comprised of two identical heavy chains (~50 kDa
each) and two identical light chains (~25 kDa each). The two
heavy chains are linked to each other and to a light chain each
by disulfide bonds, and the resulting tetramer has two identical
halves which together form the Y-like characteristic shape of the
antibody.
Affinity-based depletion strategies are often employed to
selectively remove high abundance proteins, such as HSA and
IgG, to concentrate the lower abundance proteins and facilitate
their analysis. Traditionally, antibody-based schemes are used
to produce affinity-binding ligands for HSA and IgG, where
monoclonal or polyclonal antibodies are produced in a source
animal, purified, and then bound to a surface (e.g. bead or well)
for preparation of serum samples. Variability in the production
of these antibodies leads to a wide variety of specificities and
selectivities, as the binding of the ligand to the HSA or IgG is
dependent upon the availability of the epitope, the binding affinity
of the antibody, and the homogeneity of the antibody population.
Thus, the efficiencies for antibody-based depletion strategies for
the selective removal of high abundance proteins in human serum
can vary broadly, from 70 to 95%, and can dramatically affect the
reproducibility of studies of the human serum proteome.
The ProteaPrep IgG Capture Sample Prep Kit (SP-230) utilizes
a proprietary technology that incorporates a reversible binding
recombinant affinity ligand for highly efficient capture (>99%)
and release (>80%) of IgG from plasma and serum samples in
less than 30 minutes. The ProteaPrep IgG Capture Sample Prep
Kit is effective for the capture and recovery from many species
including human, bovine, pig, and horse.
Coomassie blue stained SDS-PAGE gel showing the capture
and recovery of 250 µg human immunoglobulin G (IgG) using
the SP-230 ProteaPrep IgG Capture Sample Prep Kit. Lane
1 contains a molecular weight marker. Lanes 2 to 4 show
recovery controls based on 70%, 80%, and 90% of 250 µg of
IgG, respectively. Lanes 5 and 6 shows the eluate from 250
µg of IgG from two different vendors that was captured and
recovered with >80% efficiency using the SP-230 ProteaPrep
IgG Capture Sample Prep Kit. The gel is a 7.5% ProteaGel™
stained with Coomassie Blue G-250 for visualization of the
proteins.
FEATURES:
ORDERING INFORMATION:
•
Contains SpinTubes with functionalized beads, Bead Activation
Buffer, and IgG Release Reagent
Catalog Number
Size
Price
SP-230-12
12 SpinTubes
$425
SP-230-24
24 SpinTubes
$850
SP-230-4x24
4 x 24 SpinTubes
$3400
•
•
•
•
>99% efficiency for depletion of human IgG
>80% efficiency for recovery of human IgG
Simple and easy to use process samples in less than 30
minutes
New recombinant affinity ligand permits highly specific
and reproducible IgG removal to facilitate analysis of lower
abundance serum proteins
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
RESEARCH APPLICATIONS:
•
•
•
Intact IgG analysis and classification
Characterization of antibody production in non-human
animals
Recovery of IgG for downstream functional assessment
www.proteabio.com • 6
ProteaPrep IgG Capture Sample Prep Kit
Protein Sample Preparation
ProteaPrep Albumin and IgG Depletion Sample Prep Kit
A major problem in the analysis of the human serum proteome
is the inhibition of the detection of lower abundance proteins
by higher abundance proteins, such as albumin (HSA) and
immunoglobulin G (IgG). Together these proteins comprise ~75%
of the total serum protein content. The selective removal of these
two major serum proteins permits for a more effective analysis of
the proteome.
Affinity-based depletion strategies are often employed to
selectively remove high abundance proteins, such as HSA and
IgG, to concentrate the lower abundance proteins and facilitate
their analysis. Traditionally, antibody-based schemes are used
to produce affinity-binding ligands for HSA and IgG, where
monoclonal or polyclonal antibodies are produced in a source
animal, purified, and then bound to a surface (e.g. bead or well)
for preparation of serum samples. Variability in the production
of these antibodies leads to a wide variety of specificities and
selectivities, as the binding of the ligand to the HSA or IgG is
dependent upon the availability of the epitope, the binding affinity
of the antibody, and the homogeneity of the antibody population.
Thus, the efficiencies for antibody-based depletion strategies for
the selective removal of high abundance proteins in human serum
can vary broadly, from 70 to 95%, and can dramatically affect the
reproducibility of studies of the human serum proteome.
The ProteaPrep Albumin and IgG Depletion Sample Prep Kit
(SP-240) features a revolutionary new approach to affinity-based
serum albumin and IgG removal that utilizes a non-antibodybased method. The ProteaPrep Albumin and IgG Depletion
capture ligands are recombinant proteins that exhibit excellent
batch-to-batch reproducibility and are highly purified for robust,
efficient removal of albumin and IgG from human serum samples.
The net result is that the functionalized beads irreversibly bind
and remove the albumin and IgG from serum in a highly efficient
and rapid manner. Depletion efficiencies are >99% for the
selective removal of both albumin and IgG in a single SpinTube
from serum samples in less than 20 minutes.
FEATURES:
•
•
•
•
•
•
>99% efficiency for depletion of both human serum albumin
and IgG
Simple and easy to use – process samples in less than 20
minutes
New recombinant affinity ligand permits highly specific and
reproducible albumin and IgG removal to facilitate analysis of
lower abundance serum proteins
Disposable, cost-effective SpinTube design eliminates the
risk of carryover or sample contamination
Effectively remove albumin and IgG in one step
Preparation of serum samples for analysis by SDS-PAGE
gel electrophoresis and mass spectrometry
Coomassie blue stained SDS-PAGE gel demonstrating
>99% Depletion of human serum albumin (HSA) and IgG
from 10 µL of human serum using the SP-240 ProteaPrep
Albumin and IgG Depletion Sample Prep Kit. The upper
(A) and lower (B) black arrows indicate the IgG and HSA
protein bands, respectively, in the control sample that are not
observed in the depleted sample.
Key: Lane 1 = molecular weight markers ; Lane 2 = 10 µL
human serum control, 10 % sample load ; Lane 3 = 10 µL
human serum, HSA and IgG depleted, 10 % sample load.
ORDERING INFORMATION:
ProteaPrep Albumin and IgG Depletion
Sample Prep Kit
Contains SpinTubes with functionalized beads and Bead
Activation Buffer
Catalog Number
Size
Price
SP-240-12
12 SpinTubes
$575
SP-240-24
24 SpinTubes
$1150
SP-240-4x24
4 x 24 SpinTubes
$4600
RESEARCH APPLICATIONS:
•
•
•
Human serum proteome analysis
Depletion of albumin and IgG from serum and plasma
samples
Preparation of serum samples for analysis by SDS-PAGE,
2D gel electrophoresis, and mass spectrometry
7 • 877.776.8321
ProgentaTM Anionic Acid Labile Surfactants
Progenta Acid Labile Surfactants (ALS) are novel, acid cleavable
detergents that are fully compatible with mass spectrometry
analysis.
The Progenta Anionic Acid Labile Surfactants have been
engineered to provide a safe, robust alternative to detergents (e.g.
sodium dodecyl sulfate) that are commonly used in proteomics
work, but negatively impact subsequent analysis by mass
spectrometry. While SDS and other detergents can improve
protein solubility, they can be very difficult to remove during
sample prep and purification of the protein sample. These bound
detergents can cause significant impairment of protein analysis
by mass spectrometry, as the surfactants can suppress analyte
ion signal, promote analyte adduct formation, and present as
contaminants during the analysis.
The Progenta Anionic Acid Labile Surfactants are fully mass spec
compatible and alleviate the problems commonly associated with
SDS and other detergents in proteomics studies. At neutral pH,
the Progenta AALS functions as a powerful detergent for use in
sample preparation, protein solubilization, and cell lysis protocols.
After completing the experimental work, the solution is adjusted
to a pH of 2 – 3 with trifluoracetic acid (TFA) and incubated for 10
minutes to fully cleave the AALS into small organic molecules that
do not exhibit surfactant activity or interfere with analysis by mass
spectrometry. Other commercially available acid labile surfactants
require incubation in harsher acidic environments of pH 1.0 to 2.0
for up to several hours and may produce an oily pellet or film from
the cleavage by-products. Progenta AALS’s have been specially
engineered to provide powerful surfactant performance with the
added benefit of quick, simple acid-based detergent cleavage
and removal for reproducible results in mass spectrometry-based
proteomics studies.
Both Progenta Anionic Acid Labile Surfactants are effective at
solubilizing peptides and proteins, functioning in cell lysis protocols,
optimizing enzymatic digestions, reducing surface adsorption
losses via non-specific interactions, and acting as an alternative
to SDS. AALS I has a Critical Micelle Concentration (CMC) of 7.7
mM, and AALS II has a slightly longer hydrophobic tail, yielding a
more powerful CMC of 1.9 mM. Both AALS I and II exhibit fast,
efficient degradation kinetics at acidic pH’s of 2.0 to 3.0.
FEATURES:
•
•
•
•
•
Rapidly cleaved in just 10 minutes at pH 2.0 - 3.0 (e.g. 1%
TFA)
AALS cleavage products are fully soluble and do not interfere
with mass spectrometry analysis
Compatible with both MALDI and ESI mass spectrometry
AALS promotes protein solubility and improves enzymatic
digestion
Certified Mass Spec Grade
RESEARCH APPLICATIONS:
•
•
•
•
•
•
Solubilization of proteins and other biomolecules
Sample preparation and solid phase extraction
Improved enzymatic digestion of proteins
Gel electrophoresis and electroelution buffers
Cell lysis and protein extraction from cell lines, tissues, and
biological samples
Extraction of biomolecules from environmental samples
www.proteabio.com • 8
SPECIFICATIONS:
Progenta Anionic Acid Labile Surfactant I (AALS I)
• Critical Micellular Concentration (CMC) = 7.7 mM
• Recommended concentration: 0.01 – 2.0%
Progenta Anionic Acid Labile Surfactant II (AALS II)
• Critical Micellular Concentration (CMC) = 1.9 mM
• Recommended concentration: 0.01 – 2.0%
ORDERING INFORMATION:
Progenta Anionic Acid Labile Surfactant I (AALS I)
Catalog NumberSizePrice
ALS-100-5mg
ALS-100-5x5mg
ALS-100-10x5mg
5 mg
5x5 mg
10x5 mg
$158
$709
$1470
Progenta Anionic Acid Labile Surfactant II (AALS II)
Catalog NumberSizePrice
ALS-110-5mg
ALS-110-5x5mg
ALS-110-10x5mg
5 mg
5x5 mg
10x5 mg
$184
$814
$1470
Protein Sample Preparation
Improvement of MS Performance Using Progenta Acid Labile Surfactant
After just 10 minutes in 1% TFA, the Anionic Acid Labile Surfactant is fully cleaved into inert chemical species that do not have
surfactant properties and that do not interfere with analysis by mass spectrometry. The protein peaks (purple) are fully recovered
from the detergent-suppressed sample (green).
Publications:
Li, M., Powell, M.J., Razunguzwa, T.T., O’Doherty, G.A. A general approach to anionic acid-labile surfactants with tunable
properties. J. Org. Chem. 2010, 75, 6149–6153.
9 • 877.776.8321
ProgentaTM Zwitterionic and Cationic Acid Labile Surfactants
Progenta Zwitterionic Acid Labile Surfactants (ZALS) and
Cationic Acid Labile Surfactants (CALS) are novel, acid cleavable
detergents that are fully compatible with mass spectrometry
analysis. The Progenta ZALS and CALS have been engineered
to provide a safe alternative to detergents (e.g. CHAPS, LDAO,
respectively) that are commonly used in proteomics work, but that
negatively impact subsequent analysis by mass spectrometry.
While CHAPS and other detergents can improve protein
solubility, they can be very difficult to remove during sample prep
and purification of the protein sample. These bound detergents
can cause significant impairment of protein analysis by mass
spectrometry, as the surfactants can suppress analyte ion signal,
promote analyte adduct formation, and present as contaminants
during the analysis.
The Progenta Zwitterionic and Cationic Acid Labile Surfactants
are fully mass spec compatible and alleviate the problems
commonly associated with CHAPS and other detergents in
proteomics studies. At neutral pH, the Progenta ZALS and CALS
function as powerful detergents for use in sample preparation,
protein solubilization, and cell lysis protocols. After completing
the experimental work, the solution is adjusted to a pH of 2.0
with trifluoroacetic acid (TFA) and incubated for 30 minutes to
fully cleave the ZALS and CALS into small organic molecules
that do not exhibit surfactant activity or interfere with analysis
by mass spectrometry. Other commercially available acid labile
surfactants require incubation in harsher acidic environments
of pH 1.0 to 2.0 for up to several hours and may produce an oily
pellet or film from the cleavage by-products. Progenta ZALS and
CALS have been specifically engineered to provide powerful
surfactant performance with the added benefit of quick, simple
acid-based detergent cleavage and removal for reproducible
results in mass spectrometry-based proteomics studies.
Both Progenta Zwitterionic Acid Labile Surfactants are effective
at solubilizing peptides and proteins, functioning in cell lysis
protocols, optimizing enzymatic digestions, reducing surface
adsorption losses via non-specific interactions. The Progenta
ZALS is also effective at replacing CHAPS in the isoelectric
focusing (IEF) step of 2D gel separations. Both ZALS I and
CALS I have longer hydrophobic tails that provide a powerful
CMC, while ZALS II and CALS II have shorter hydrophobic tails,
yielding a less powerful CMC. All formulations of ZALS and CALS
exhibit fast, efficient degradation kinetics at acidic pH’s of 2.0.
FEATURES:
•
•
•
•
Rapidly cleaved in 30 minutes at pH 2.0 – 2.5 (e.g., 1% TFA)
Cleavage products are fully soluble and can be washed
away to not interfere with mass spectrometry analysis
Promotes protein solubility and improves enzymatic
digestion
Certified Mass Spec Grade
RESEARCH APPLICATIONS:
•
•
•
•
•
•
•
Solubilization of proteins and other biomolecules
Sample preparation and solid phase extraction
Improved enzymatic digestion of proteins
Cell lysis and protein extraction from cell lines, tissues, and
biological samples
2D gel electrophoresis buffers
Reversed polarity gel electrophoresis and electroelution
buffers
Extraction of biomolecules from environmental samples
www.proteabio.com • 10
SPECIFICATIONS:
Progenta Zwitterionic Acid Labile Surfactant I (ZALS I)
• Critical Micellular Concentration (CMC) = 3.4 mM
• Recommended concentrations: 0.01 - 0.1%
Progenta Zwitterionic Acid Labile Surfactant II (ZALS II)
• Critical Micelluar Concentration (CMC) = 31.3 mM
• Recommended concentrations: 0.01-0.1%
Progenta Cationic Acid Labile Surfactant I (CALS I)
• Critical Micellular Concentration (CMC) = 3.5 mM
• Recommended concentrations: 0.01-1.0%
Progenta Cationic Acid Labile Surfactant II (CALS II)
• Critical Micellular Concentration (CMC) = 12.8 mM
• Recommended concentrations: 0.01-1.0%
ORDERING INFORMATION:
Progenta Zwitterionic Acid Labile Surfactant I
(ZALS I)
Catalog NumberSizePrice
ALS-200-5mg
ALS-200-5x5mg
ALS-200-10x5mg
5 mg
5x5 mg
10x5 mg
$184
$814
$1470
Progenta Zwitterionic Acid Labile Surfactant II (ZALS II)
Catalog NumberSizePrice
ALS-210-5mg
ALS-210-5x5mg
ALS-210-10x5mg
5 mg
5x5 mg
10x5 mg
$152
$677
$1202
Progenta Cationic Acid Labile Surfactant I (CALS I)
Catalog NumberSizePrice
ALS-300-5mg
ALS-300-5x5mg
ALS-300-10x5mg
5 mg
5x5 mg
10x5 mg
$184
$814
$1470
Progenta Cationic Acid Labile Surfactant II (CALS II)
Catalog NumberSizePrice
ALS-310-5mg
ALS-310-5x5mg
ALS-310-10x5mg
5 mg
5x5 mg
10x5 mg
$152
$677
$1202
ORDERING INFORMATION:
Progenta Acid Labile Surfactant Assortment I
Progenta Acid Labile Surfactant Assortment III
Contains 5 mg each of the Progenta Anionic Acid Labile
Surfactants [AALS] I and II (ALS-100 and ALS-110), the Cationic
Acid Labile Surfactants [CALS] I and II (ALS-300 annd ALS-310),
and the Zwitterionic Acid Labile Surfactants [ZALS] I and II (ALS200 and ALS-210)
Catalog NumberSizePrice
Catalog NumberSizePrice
ALS-500
1 pack
$866
Progenta Acid Labile Surfactant Assortment II
Contains 5 mg each of the Progenta Anionic Acid Labile
Surfactants [AALS] I and II (ALS-100 and ALS-110) and the
Zwitterionic Acid Labile Surfactants [ZALS] I and II (ALS-200 and
ALS-210)
Catalog NumberSizePrice
ALS-501
1 pack
$572
Contains 5 mg each of the Progenta Anionic Acid Labile
Surfactants [AALS] I and II (ALS-100 and ALS-110) and the
Cationic Acid Labile Surfactants [CALS] I and II (ALS-300 and
ALS-310)
ALS-502
1 pack
$572
Progenta Acid Labile Surfactant Assortment IV
Contains 5 mg each of the Progenta Cationic Acid Labile
Surfactants [CALS] I and II (ALS-300 and ALS-310) and the
Zwitterionic Acid Labile Surfactants [ZALS] I and II (ALS-200 and
ALS-210)
Catalog NumberSizePrice
ALS-503
1 pack
$572
Publications:
Li, M., Powell, M.J., Razunguzwa, T.T., O’Doherty, G.A. A general approach to anionic acid-labile surfactants with tunable
properties. J. Org. Chem. 2010, 75, 6149–6153.
11 • 877.776.8321
Protein Sample Preparation
ProgentaTM Acid Labile Surfactant Kits
ProgentaTM Acid Labile Surfactant Selection Guide
Anionic Acid Labile Surfactant
Product Information
AALS I
Common Detergents for Replacement by Progenta Acid
Labile Surfactants
CMC
AALS II
sodium dodecyl sulfate (SDS), ammonium lauryl sulfate,
perfluorooctonoate, sodium cholate, sodium deoxycholate
7.7 mM
1.9 mM
0.01 - 2.0%
0.01 - 2.0%
2.0 - 3.0
2.0 - 3.0
1% TFA;
5-10% Formic Acid
1% TFA;
5-10% Formic Acid
Negative
Negative
Protein solubilization
X
X
Cell lysis
X
X
Sample preparation and solid phase extraction
X
X
Enzymatic digestions
X
X
Electrophoresis buffers
X
X
Electroelution buffers
X
X
Immunoprecipitation
X
X
Individual Part Numbers
ALS-100-5mg
ALS-100-5x5mg
ALS-100-10x5mg
ALS-110-5mg
ALS-110-5x5mg
ALS-110-10x5mg
Assortment Part Numbers
ALS-500
ALS-501
ALS-502
ALS-500
ALS-501
ALS-502
Recommended concentration
pH for surfactant cleavage
Degradation reagent
Charge
Application
2D gel electrophoresis buffers
Reversed polarity electrophoresis and electroelution
www.proteabio.com • 12
ZALS I
Cationic Acid Labile Surfactant
ZALS II
CHAPS, CHAPSO, dodecyl betaine,
cocamidopropyl betaine, coco ampnoglycinate
CALS I
CALS II
lauryl pyridinium chloride,
lauryldimethylamine-oxide (LDAO),
cetyltrimethylammonium bromide (CTAB)
3.4 mM
31.3 mM
3.5 mM
12.8 mM
0.01 - 0.1%
0.01 - 0.1%
0.01 - 1.0%
0.01 - 1.0%
2.0 - 2.5
2.0 - 2.5
2.0 - 2.5
2.0 - 2.5
1% TFA;
5-10% Formic Acid
1% TFA;
5-10% Formic Acid
1% TFA;
5-10% Formic Acid
1% TFA;
5-10% Formic Acid
Net Zero
Net Zero
Positive
Positive
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
ALS-200-5mg
ALS-200-5x5mg
ALS-200-10x5mg
ALS-210-5mg
ALS-210-5x5mg
ALS-210-10x5mg
ALS-300-5mg
ALS-300-5x5mg
ALS-300-10x5mg
ALS-310-5mg
ALS-310-5x5mg
ALS-310-10x5mg
ALS-500
ALS-501
ALS-503
ALS-500
ALS-501
ALS-503
ALS-500
ALS-502
ALS-503
ALS-500
ALS-502
ALS-503
13 • 877.776.8321
Protein Sample Preparation
Zwitterionic Acid Labile Surfactant
ProteaPrepTM Cell Lysis Kit, Mass Spectrometry Grade
The ProteaPrep Cell Lysis Kit, Mass Spectrometry Grade has
been specially designed for the efficient recovery of purified
protein lysates from biological samples. The ProteaPrep Cell
Lysis Kit is a proprietary formulation containing a mixture of salts,
glycerol, and an acid labile surfactant that has been optimized
for efficient solubilization, extraction, and recovery of proteins
during cell lysis. Because the ProteaPrep Cell Lysis Kit is free
of lysozyme and harsh detergents (e.g. SDS), the product
permits recovery of a cell lysate that is free from artifactual
protein contaminants and promotes efficient sample protein
solubilization.
The ProteaPrep Cell Lysis Kit features the ProgentaTM Anionic
Acid Labile Surfactant II (AALS II), a powerful acid cleavable
detergent that alleviates the problems commonly associated
with SDS and other detergents in proteomics studies. After
completing the cell lysis, the cell extract solution can be adjusted
to a pH of 2–3 (by TFA, formic acid, etc.) and incubated for 10-20
minutes to fully cleave the AALS into small organic molecules
that do not exhibit surfactant activity or interfere with downstream
sample preparation and analysis by techniques such as mass
spectrometry, enzymatic digestions, 2D gel electrophoresis, and
protein quantitation (e.g. Bradford and Lowry assays).
FEATURES:
•
•
•
Cell lysate is free of lysozyme and harsh denaturing
detergents (e.g. SDS)
Proprietary anionic acid labile surfactant promotes
solubilization of sample proteins with the benefit of rapid
detergent degradation under acidic conditions
Compatible with mass spectrometry, enzymatic digestions,
2D gel electrophoresis, and protein quantitation (e.g.
Bradford and Lowry assays)
RESEARCH APPLICATIONS:
•
•
•
•
•
Protein extraction and purification from cell lines, cultures,
and tissues
Purification of biologically active proteins and enzymes
Shotgun proteomics analysis of complex protein samples
Analysis of biological samples by 2D gel electrophoresis
Extraction of proteins for immunoblot and ELISA analysis
ORDERING INFORMATION:
ProteaPrep Cell Lysis Kit, Mass Spectrometry Grade
Catalog NumberSizePrice
SP-810-10mL
SP-810-25mL
10 mL
25 mL
$152
$362
Bacterial cells from BL21 E. coli strain were lysed
using the ProteaPrep Cell Lysis Kit (SP-810).
Following degradation of the AALS II detergent,
40 µg of lysate was separated using isoelectric
focusing on a pH 4-7 IPG strip. The sample was then
separated by SDS-PAGE on a 10% ProteaGel
(PG-402D) and stained using Protea’s Silver Stain
Kit (SS-100). Over 200 protein spots are clearly
resolved on the gel.
www.proteabio.com • 14
Protein Sample Preparation
ProteaPrep Protease Inhibitor Cocktail
The ProteaPrep Protease Inhibitor Cocktail is an easy-to-use
reagent for the inhibition or protease activity during biological
sample preparation to prevent degradation of sample proteins.
We recommend that the ProteaPrep Protease Inhibitor Cocktail
be used with our ProteaPrep Cell Lysis Kit, Mass Spec Grade
(SP-810) for the rapid and efficient recovery or proteins from cell
cultures, lines, and tissue specimens. The Protease Inhibitor
Cocktail is provided as a lyophilized powder for a long shelf life
and simple, rapid reconstitution and usage.
FEATURES:
•
•
Ready-to-use lyophilized powder permits quick and easy
preparation of protease inhibitor cocktail mix
Contains reversible and irreversible inhibitors of
aminopeptidases and serine-, cysteine-, metallo-, and
aspartic acid- proteases
RESEARCH APPLICATIONS:
•
•
•
Protein extraction and purification from cell lines, cultures,
and tissues where further analysis will be conducted
Purification of biologically active proteins and enzymes
Extraction of proteins for 2-D gels, Immunoblot and ELISA
analysis
ORDERING INFORMATION:
ProteaPrep Protease Inhibitor Cocktail
Contains Lyophilized powder, pack of 5 vials (reconstitute to 1.0
mL of 10X concentrate per vial)
Catalog NumberSizePrice
SP-820
1 pack
$32
Bacterial cell lysates from BL21 E.coli strain were prepared
using ProteaPrep Bacterial and Mammalian Cell Lysis Kit. 100
µL of cell lystate containing approximately 5 mg/mL total protein
was tested as shown below. Samples incubated overnight at
37°C for tryptic digestion. After denaturation, samples (10 µL
each) were separated on a 10% SDS-PAGE gel and visualized
by Coomassie R250 staining. Lane B demonstrates tryptic
digestion of the sample (compare to Lane A). Lane C shows
the inhibition of trypsin by the ProteaPrep Protease Inhibitor
Cocktail.
ProteaPrep Coomassie Protein Quantitation Kit
The ProteaPrep Coomassie Protein Quantitation Kit is a
colorimetric assay for simple and reproducible determination of
protein concentration in 10 minutes. The kit utilizes a modified
Bradford method that measures absorbance at 595 nm and
results in significantly less protein-to-protein variation than is
observed with other Bradford-based Coomassie assays. The
ProteaPrep Coomassie Quantitation kit has a linear working
range for BSA from 1 to 1500 µg/mL (0.001 to 1.5 µg/µL). Each
kit contains 250 mL of Coomassie quanititation reagent and an
albumin standard for generation of the standard curve, and the kit
can be used in either a microplate or cuvette format.
FEATURES:
•
•
•
Sample is ready for quantitation in 10 minutes after reagent
is added
Large range in working concentrations
Low protein-to-protein variation
RESEARCH APPLICATIONS:
•
•
•
Protein sample quantitation
Cell lysis protocols
Proteomics applications
ORDERING INFORMATION:
ProteaPrep Coomassie Protein Quantitation Kit
Catalog NumberSizePrice
SP-880-1
1 kit
$142
15 • 877.776.8321
Endoproteinases for Protein Digestion - Proteases
Proteomics applications require high-performance enzymes for
efficient and reproducible digestion of proteins. Protea offers a
selection of the most commonly used proteases in proteomics
work. Each protease is a high-quality, purified enzyme that is
batch-tested for quality assurance of specificity and performance.
FEATURES:
•
•
•
Certified Mass Spec Grade enzyme for proteomics
applications
Convenient aliquots for quick digestion and sample prep
Quality Assurance tested for specificity and performance
RESEARCH APPLICATIONS:
•
•
•
Protein identification and characterization by LC-MS/MS
De novo sequencing of unique proteins
Peptide mass fingerprinting by MALDI-MS
ORDERING INFORMATION:
PROTEASES:
Trypsin
Chymotrypsin
Certified MS Grade, 1 pack of 5 vials, 10 µg/vial (50 µg total)
Catalog NumberSizePrice
PE-101
1 pack
$53
Certified MS Grade, 1 pack of 4 vials, 5 µg/vial (20 µg total)
Catalog NumberSizePrice
PE-104
1 pack
$74
Endoproteinase Glu-C
Endoproteinase Arg-C
Certified MS Grade, 1 pack of 5 vials, 10 µg/vial (50 µg total)
Certified MS Grade, 1 pack of 5 vials, 10 µg/vial (50 µg total)
PE-102
PE-105
Catalog NumberSizePrice
1 pack
$74
Catalog NumberSizePrice
1 pack
$74
Pepsin
Certified MS Grade, 1 pack of 5 vials, 10 µg/vial (50 µg total)
Catalog NumberSizePrice
PE-103
1 pack
$74
Enzyme
Source
Specificity
Digestion Conditions
Trypsin
Bovine pancreas
Carboxy side of arginine (R)
and lysine (L) bonds
50 mM ammonium
bicarbonate, pH 8.0, 0 to
20% acetonitrile, 37°C
Glu-C (V8 protease)
Staphylococcus aureus V8
Carboxy side of glutamic
acid 50 mM (E) and aspartic
acid (D) bonds
Ammonium bicarbonate,
pH 7.8, 30-37°C
Chymotrypsin
Bovine pancreas
Carboxy side of tyrosine
(Y), tryptophan (W), and
phenylalanine (F) bonds
50 mM ammonium
bicarbonate, pH 8.0, 1 mM
calcium chloride, 0 to 20%
acetonitrile , 30-37°C
Arg-C (Clostripain)
Clostridium histolyticum
Carboxy side of arginine (R)
bonds
50 mM ammonium
bicarbonate, pH 7.8, 5 mM
dithiothreitol (DTT), 1 mM
calcium chloride, 30-37°C
Pepsin
Porcine stomach
Carboxy side of tyrosine
(Y), Tryptophan (W),
Penylalanine (F), and
Leucine (L) bonds
10 mM hydrochloric acid,
pH 1-3, 37°C
www.proteabio.com • 16
Protein Sample Preparation
Protein Digestion Kits
ORDERING INFORMATION:
Trypsin Digestion Kit
Certified MS Grade
1 pack of 5 vials, 10 µg/vial trypsin (50 µg total), with 10X
digestion buffer, reduction reagent (diothiothreitol), and alkylation
reagent (iodoacetamide)
Catalog NumberSizePrice
PE-151
1 Kit
$135
Glu-C Digestion Kit
Certified MS Grade
1 pack of 5 vials, 10 µg/vial Glu-C (50 µg total), with 10X
digestion buffer, reduction reagent (dithiothreitol), and alkylation
reagent (iodoacetamide)
Catalog NumberSizePrice
PE-152
1 Kit
$135
Chymotrypsin Digestion Kit
Certified MS Grade
1 pack of 4 vials, 5 µg/vial chymotrypsin (20 µg total), with 10X
digestion buffer, reduction reagent (dithiothreitol), and alkylation
reagent (iodoacetamide)
Catalog NumberSizePrice
PE-154
1 Kit
$135
Endoproteinase Arg-C Digestion Kit
Certified MS Grade
1 pack of 5 vials, 10 µg/vial Arg-C (50 µg total), with 10X
digestion buffer, and reduction reagent (dithiothreitol)
Catalog NumberSizePrice
PE-155
1 Kit
$135
Chromatograms of trypsin-treated, and untreated ACTH
(1-14) peptides, separated on C8 reversed phase column.
Panel A shows undigested, 14 amino acid long ACTH peptide
(SYSMEHFRGKPVG), represented by a peak at 10.23
minutes. Panel B shows peaks at 7.33 and 8.73 minutes that
correspond to the 6 amino acid and 8 amino acid peptides
from trypsin cleavage, respectively.
Pepsin Digestion Kit
Certified MS Grade
1 pack of 5 vials of MS Grade Pepsin (each vial contains 10 µg)
and 1 vial of 10x Pepsin Digestion Buffer (2 mL)
Catalog NumberSizePrice
PE-153
1 Kit
$135
C8 reversed phase chromatographic seperation of ACTH (114) peptide substrate treated with Glu-C Protease. Peak at
10.1 minutes corresponds to undigested ACTH peptide of 14
amino acids. Peaks at 4.9 and at 9.4 minutes correspond to
the 5 amino acid and 9 amino acid peptides respectively.
17 • 877.776.8321
Ultrapure CHCA MALDI Matrix
Ultrapure α-cyano-4-hydroxycinnamic acid (CHCA) is a
recrystallized matrix for matrix-assisted laser desorption
ionization (MALDI) mass spectrometry. Ultrapure CHCA matrix
produces homogeneous crystals that lead to MALDI mass
spectra with excellent resolution, high sensitivity, and high S/N
ratios.
RESEARCH APPLICATIONS:
FEATURES:
Ultrapure CHCA MALDI Matrix
•
•
•
Improved MALDI–MS sensitivity (higher S/N)
Forms homogeneous crystals resulting in excellent
resolution
Easy reconstitution directly in vial for ready-to-use stock
•
•
MALDI mass spectrometry analysis of peptides and small
proteins
Post-source decay experiments
ORDERING INFORMATION:
Catalog NumberSizePrice
CMS-100-50mg
CMS-100-100mg
50 mg
100 mg
$68
$126
Ultrapure Sinapic Acid MALDI Matrix
Ultrapure Sinapic Acid is a recrystallized matrix for matrixassisted laser desorption ionization (MALDI) mass spectrometry.
The improved purity enhances the MALDI–MS signal sensitivity
and S/N ratio.
RESEARCH APPLICATIONS:
FEATURES:
Ultrapure Sinapic Acid MALDI Matrix
•
•
Improved MALDI–MS sensitivity (higher S/N)
Easy reconstitution directly in vial for ready-to-use stock
•
Analysis of high molecular weight proteins
ORDERING INFORMATION:
Catalog Number
CMS-101-50mg
CMS-101-100mg
Size
50 mg
100 mg
Price
$68
$126
Ultrapure DHB MALDI Matrix
Ultrapure 2, 5-dihydroxybenzoic acid (DHB) is a recrystallized
matrix for matrix-assisted laser desorption ionization (MALDI)
mass spectrometry. The improved purity enhances the MALDI–
MS signal sensitivity and S/N ratio.
FEATURES:
•
•
•
Improved MALDI–MS sensitivity (higher S/N)
Easy reconstitution directly in vial for ready-to-use stock
Higher tolerance to salts and detergents in the sample
compared to other matrices
www.proteabio.com • 18
RESEARCH APPLICATIONS:
•
•
Analysis of carbohydrates and glycoproteins
Peptide analysis in ISD (In-Source Decay) experiments
ORDERING INFORMATION:
Ultrapure DHB MALDI Matrix
Catalog Number
CMS-102-50mg
CMS-102-100mg
Size
50 mg
100 mg
Price
$68
$126
Ultrapure 2,6-dihydroxyacetophenone (DHAP) is a recrystallized
matrix for matrix assisted laser desorption ionization (MALDI)
mass spectrometry. DHAP is the matrix of choice for protein
profiling using MALDI-TOF mass spectrometry, and it has a
reduced sensitivity to the presence of salts in protein samples.
FEATURES:
•
•
Easy reconstitution directly in vial for ready-to-use stock
Higher tolerance to the presence of salts in the protein
sample for protein profiling (compared to CHCA and Sinapic
acid)
RESEARCH APPLICATIONS:
•
•
Protein profiling of complex protein samples (e.g. serum)
Mass spectrometry analysis of protein samples with higher
salt content
ORDERING INFORMATION:
Ultrapure DHAP MALDI Matrix
Catalog NumberSizePrice
CMS-103-50mg
CMS-103-100mg
50 mg
100 mg
$68
$126
Ultrapure DAN MALDI Matrix
Ultrapure 1,5-diaminonapthalene (DAN) is an important matrix
used in peptide and protein analysis by matrix assisted laser
desorption ionization (MALDI) mass spectrometry. The matrix
is effective in promoting reduction of disulfide bonds in the gas
phase, which greatly facilitates analysis of proteins and peptides
containing disulfide linkages.
FEATURES:
•
•
•
Improves CID fragmentation of proteins with disulfide
linkages
Enhances in-source fragmentation of peptides
Easy reconstitution directly in vial for ready-to-use stock
RESEARCH APPLICATIONS:
•
•
•
MS and MS/MS analysis of peptides and proteins with
disulfide linkages
Peptide analysis in ISD (In Source Decay) experiments
Analysis of gangliosides by MALDI-MS
ORDERING INFORMATION:
Ultrapure DAN MALDI Matrix
Catalog NumberSizePrice
CMS-104-50mg
CMS-104-100mg
50 mg
100 mg
$68
$126
Ultrapure 3-HPA MALDI Matrix
The Ultrapure 3-Hydroxypicolinic Acid (3-HPA) is a matrix for
use in the analysis of oligonucleotides by matrix assisted laser
desorption ionization (MALDI) mass spectrometry. The matrix is
recrystallized for maximizing S/N and minimizing salt adducts.
RESEARCH APPLICATIONS:
FEATURES:
ORDERING INFORMATION:
•
•
•
Convenient pre-weighed 50 mg vials
Reconstitution solution can be added directly to vial
Good matrices for oligonucleotides with lengths between
2-50 bases
•
•
Analysis of oligonucleotides from molecular biology assays
Detection of length variations in DNA nucleotide sequence
repeats
Ultrapure 3-HPA MALDI Matrix
Catalog NumberSizePrice
CMS-105-100mg
CMS-105-250mg
100 mg
250 mg
$68
$126
19 • 877.776.8321
Protein Sample Preparation
Ultrapure DHAP MALDI Matrix
MALDI Matrix Selection Guide
Common
Abbreviation
CHCA
Sinapic Acid
DHB
DHAP
Chemical Name
α-cyano-4hydroxycinnamic
acid
sinapic acid
2,5-dihydroxybenzoic acid
2,6-dihydroxyacetophenone
Molecular
Weight
189.17
224.21
154.12
152.15
CAS Number*
28166-41-8
530-59-6
490-79-9
89-84-9
Sample
Compatibility/
Application
Peptides
X
Small Proteins
X
High Molecular
Weight Proteins
X
Carbohydrates
X
Glycoproteins
X
Complex Protein
Sample Profiling
X
Gangliosides
Oligonucleotides
Glycolipids
Phospholipids
Synthetic
Polymers
Peptides with
Disulfide
Linkages
Chloroamine
Detection
MS with High
Salt Content
X
In-Source Decay
Experiments
Part Numbers
X
CMS-100-50mg
CMS-100-100mg
CMS-101-50mg
CMS-101-100mg
CMS-102-50mg
CMS-102-100mg
*CAS Registry Number is a Registered Trademark of the American Chemical Society
www.proteabio.com • 20
CMS-103-50mg
CMS-103-100mg
3-HPA
THAP
HABA
MBT
1,5-diamino
napthalene
3-hydroxypicolinic
acid
2,4,6-trihydroxyacetophenone
2-(4’-hydroxy
benzeneazo)
benzoic acid
2-mercapto
benzothiazole
158.2
139.11
152.15
242.23
167.25
2243-62-1
874-24-8
480-66-0
1634-82-8
149-30-4
Protein Sample Preparation
DAN
X
X
X
X
X
X
X
X
X
X
CMS-107-50mg
CMS-107-100mg
CMS-108-50mg
CMS-108-100mg
X
X
CMS-104-50mg
CMS-104-100mg
CMS-105-100mg
CMS-105-250mg
CMS-106-180mg
CMS-106-450mg
21 • 877.776.8321
Ultrapure THAP MALDI Matrix
Ultrapure 2,4,6-Trihydroxyacetophenone (THAP), is a
recrystallized matrix for matrix assisted laser desorption
ionization (MALDI) mass spectrometry. The matrix is useful for
analysis of oligonucleotides and glycoproteins by MALDI-TOF
mass spectrometry.
RESEARCH APPLICATIONS:
FEATURES:
Ultrapure THAP MALDI Matrix
•
•
•
Reconstitution solution can be added directly to vial
Forms MALDI spots with homogenously distributed crystals
Effective for MALDI-TOF analysis of oligonucleotides
composed of less than 50 bases
•
•
MALDI-TOF-MS analysis of oligonucleotides
Analysis of glycoproteins
ORDERING INFORMATION:
Catalog NumberSizePrice
CMS-106-180mg
CMS-106-450mg
180 mg
450 mg
$68
$126
Ultrapure HABA MALDI Matrix
Ultrapure 2-(4’-Hydroxybenzeneazo) benzoic acid (HABA),
is a recrystallized matrix for matrix assisted laser desorption
ionization (MALDI) mass spectrometry. The matrix is useful for
analysis of glycoproteins, glycolipids and synthetic polymers.
FEATURES:
•
•
Reconstitution solution can be added directly to vial
Forms MALdi spots with homogenously distributed crystals
RESEARCH APPLICATIONS:
•
•
MALDI-MS analysis of glycoproteins and glycolipids
MALDI-MS analysis of synthetic polymers
ORDERING INFORMATION:
Ultrapure HABA MALDI Matrix
Catalog NumberSizePrice
CMS-107-50mg
CMS-107-100mg
50 mg
100 mg
$68
$126
Ultrapure MBT MALDI Matrix
Ultrapure 2-Mercaptobenzothiazole (MBT) is a recrystallized
matrix for matrix assisted laser desorption ionization (MALDI)
mass spectrometry. The matrix is useful for analysis of peptides
and proteins with performance comparable to CHCA and Sinapic
acid but with a higher tolerance to surfactants such as SDS in the
sample.
RESEARCH APPLICATIONS:
FEATURES:
Catalog NumberSizePrice
•
•
•
Reconstitution solution can be added directly to vial
Forms MALDI spots with homogenously distributed crystals
Effective for MALDI-TOF analysis of proteins, peptides and
synthetic polymers
www.proteabio.com • 22
•
•
MALDI-TOF-MS analysis of proteins/peptides
Analysis of synthetic polymers
ORDERING INFORMATION:
Ultrapure MBT MALDI Matrix
CMS-108-50mg
CMS-108-100mg
50 mg
100 mg
$68
$126
Protein Sample Preparation
Reversed Phase Sample Preparation Products - C18 ProteaTips
The C18 ProteaTips Sample Prep Kit contains tips and reagents
for desalting of peptide samples for MALDI- and ESI-MS. The
kit is effective in removing salts from protein digests resulting in
mass spectra with improved S/N and reduced adducts.
The SP-129 kit contains C18 ProteaTips for 10-200 µL sample
volumes, Sample Reconstitution and Rinse Solution, Equilibration
Solution, and Elution Solution. The C18 beads are immobilized on
the inner surface of a pipette tip providing a large surface area for
sample capture.
FEATURES:
•
•
•
Effective in removal of salts that reduce S/N of peptide mass
spectra
Low backpressure during sample processing
Binding capacity: 2-4 µg
RESEARCH APPLICATIONS:
•
•
•
Effective desalting and detergent removal from biomolecule
samples
Clean-up of peptides from in-gel digested proteins
See selection chart on page 33 for assitance selecting the
correct product for your application.
ORDERING INFORMATION:
C18 ProteaTip Sample Prep Kit
10-200µL Tips. Kit contains C18 ProteaTips, Sample
Reconstitution and Rinse Solution, Equilibration Solution, and
Elution Solution
Catalog NumberSizePrice
SP-129-24
24 Tips
$68
SP-129-96
96 Tips
$215
C18 SpinTips Sample Prep Kit
The C18 SpinTips Sample Prep Kit contains tips and reagents for
reversed phase solid phase extraction of peptide samples for
electrospray or MALDI mass spectrometry, and are particularly
useful where runs of large amounts of sample solution are
involved. Sample solutions of up to 1 mL and 1000 µg total
peptide can be processed in our SpinTips. The kit is effective in
concentrating and desalting small and large proteins resulting in
mass spectra with better S/N and reduced salt adducts.
The SP-150-24 kit contains 24 C18 SpinTips for 50-1000 µL
volumes, 25 mL Sample Reconstitution and Rinse Solution,
25 mL Equilibration Solution, and 25 mL Elution Solution.
The SP-150-96 kit contains 96 C18 SpinTips for 50-1000 µL
volumes, 50 mL Sample Reconstitution and Rinse Solution,
50 mL Equilibration Solution, and 50 mL Elution Solution.
FEATURES:
•
•
For high throughput sample preparation applications
Greater Binding capacity: 1000 µg / tip (50 to 1000 µL
sample solution)
RESEARCH APPLICATIONS:
•
•
•
•
Effective desalting of biomolecule samples
Clean-up of peptides from in-gel protein digests
Fractionation of complex peptide samples
See selection chart on page 33 for assitance selecting the
correct product for your application.
ORDERING INFORMATION:
C18 SpinTips Sample Prep Kit
Kit contains C18 SpinTips, Sample Reconstitution and Rinse
Solution, Equilibration Solution, and Elution Solution
Catalog NumberSizePrice
SP-150-24
SP-150-96
24 Tips
96 Tips
$131
$394
23 • 877.776.8321
C4 and C8 SpinTips Sample Prep Kit
Protea’s C 4 and C8 SpinTips Sample Prep Kits contain tips and
reagents for reversed phase solid phase extraction of samples
containing small to large proteins, respectively, for electrospray
or MALDI mass spectrometry, and are particularly useful where
runs of large amounts of solution are involved. Sample solutions of
up to 1 mL and 1000 µg peptide/protein can be processed in our
SpinTips. The kit is effective in concentrating and desalting small
and large proteins resulting in mass spectra with better S/N and
reduced salt adducts.
FEATURES:
•
•
For high throughput sample preparation applications
Greater binding capacity: 1000 µg/tip (50 to 1000 µL sample
solution)
RESEARCH APPLICATIONS:
•
•
•
•
•
•
Effective desalting of samples containing small to large
proteins
Desalting proteins from cell lysates
Concentration of proteins extracted from dilute solutions
Clean-up of proteins electroeluted from polyacrylamide gels
Fractionation of protein mixtures
See selection chart on page 33 for assitance selecting the
correct product for your application.
C4 SpinTips Sample Prep Kit
C8 SpinTips Sample Prep Kit
The SP-152-24 kit contains 24 C 4 SpinTips for 50-1000 µL
volumes, 25 mL Sample Reconstitution and Rinse Solution,
25 mL Equilibration Solution, and 25 mL Elution Solution.
The SP-151-24 kit contains 24 C8 SpinTips for 50-1000 µL
volumes, 25 mL Sample Reconstitution and Rinse Solution,
25 mL Equilibration Solution, and 25 mL Elution Solution.
The SP-152-96 kit contains 96 C 4 SpinTips for 50-1000 µL
volumes, 50 mL Sample Reconstitution and Rinse Solution,
50 mL Equilibration Solution, and 50 mL Elution Solution.
The SP-151-96 kit contains 96 C8 SpinTips for 50-1000 µL
volumes, 50 mL Sample Reconstitution and Rinse Solution,
50 mL Equilibration Solution, and 50 mL Elution Solution.
ORDERING INFORMATION:
ORDERING INFORMATION:
Kit contains C 4 SpinTips, Sample Reconstitution and Rinse
Solution, Equilibration Solution, and Elution Solution
Kit contains C8 SpinTips, Sample Reconstitution and Rinse
Solution, Equilibration Solution, and Elution Solution
SP-152-24
SP-152-96
SP-151-24
SP-151-96
C4 SpinTips Sample Prep Kit
Catalog NumberSizePrice
24 Tips
96 Tips
www.proteabio.com • 24
$131
$394
C8 SpinTips Sample Prep Kit
Catalog NumberSizePrice
24 Tips
96 Tips
$131
$394
Protein Sample Preparation
ProteaTip ZIC® HILIC Sample Prep Kit
The ZIC ® -HILIC ProteaTip Sample Prep Kit contains tips and
reagents for clean-up of peptide samples for MALDI-MS. The kit
is effective in removing salts and detergents from protein digests,
resulting in MALDI-MS spectra with better S/N, and removes
contaminant peaks that make protein identification ambiguous.
The SP-120 kit contains ZIC ® -HILIC ProteaTips for
1-10 µL volumes, Sample Reconstitution Solution, Equilibration
and Rinse Solution, and Elution Solution.
The SP-121 kit contains ZIC ® HILIC ProteaTips for 10-200 µL
volumes, Sample Reconstitution Solution, Equilibration and Rinse
Solution, and Elution Solution.
FEATURES:
•
•
Effective in removal of detergents and salts
Enriches peptides complementary to those trapped by
C18 tips
Low backpressure during sample processing
•
RESEARCH APPLICATIONS:
•
•
•
Clean-up of in-gel protein digests
Desalting and detergent removal for peptide mixtures
See selection chart on page 33 for assitance selecting the
correct product for your application
ORDERING INFORMATION:
ProteaTip ZIC® -HILIC and Sample Prep Kit
1-10 µL Tips. Kit contains ZIC ® -HILIC ProteaTips, Sample
Reconstitution Solution, Equilibration and Rinse Solution, and
Elution Solution
Catalog NumberSizePrice
SP-120-24
24 Tips
$89
SP-120-96
96 Tips
$289
ProteaTip ZIC® -HILIC and Sample Prep Kit
10-200 µL Tips. Kit contains ZIC ® -HILIC ProteaTips, Sample
Reconstitution Solution, Equilibration and Rinse Solution, and
Elution Solution
Catalog NumberSizePrice
SP-121-24
SP-121-96
24 Tips
96 Tips
$100
$310
Clean-up of an in-gel digested cytochrome C sample
Comparison of cytochrome C digest peptides detected for ZIC ® -HILIC ProteaTips and Millipore C18 ZipTip ® processed
digests. Peptides identified by both products are highlighted in green. With the ZIC ® -HILIC ProteaTips there were 8
peptides detected that were not observed when a C18 clean-up was used.
ProteaTip ZIC® -HILIC
cleaned digest
KYIPGTK
NKGITWK
GDVEKGKK
MIFAGIKK
GKKIFVQK
CAQCHTVEK
TGPNLHGLFGR
TGPNLHGLFGRK TEREDLIAYLK
KTEREDLIAYLK
TEREDLIAYLKK KTGQAPGFTYTDANK
TGQAPGFTYT DANKNK KTGQAPGFTYTDANKNK
Peptide
Mass
805.9
846.0
859.9
907.1
947.1
1018.1
1168.3
1296.4
1350.5
1478.7
1478.7
1598.7
1712.8
1841.0
Millipore C18
cleaned digest
TGPNLHGLFGR
TGPNLHGLFGRK TEREDLIAYLK
HKTGPNLHGL FGR KTEREDLIAYLK TEREDLIAYLKK KTGQAPGFTYTDANK GITWKEETLM EYLENPK GITWKEETLM EYLENPKK
Peptide
Mass
1168.3
1296.4
1350.5
1433.6
1478.7
1478.7
1598.7
2081.3
2209.5
25 • 877.776.8321
TiO2 ProteaTip Sample Prep Kit
The Titanium Dioxide (TiO2) ProteaTip Sample Prep Kit
contains tips and reagents for the selective enrichment of
phosphopeptides from protein digest samples. The reagent
solutions supplied with Protea’s TiO2 Sample Prep Kits are
specially formulated to maximize the retention of phosphorylated
peptides, while simultaneously minimizing the retention of nonphosphorylated species.
The SP-124 kit contains TiO2 ProteaTips for 1-10 µL sample
volumes, Sample Reconstitution and Wash #1 Solution, Wash #2
Solution, and Elution Solution.
The SP-125 kit has a larger functionalized tip size for processing
10-200 µL sample volumes.
FEATURES:
•
•
Selective enrichment of phosphopeptides in protein digest
samples
Low backpressure during sample processing
RESEARCH APPLICATIONS:
•
•
•
Selective enrichment of phosphopeptides from in-gel
digested proteins for identification of protein phosphorylation
sites by mass spectrometry
Selective enrichment of phosphopeptides from complex
protein/peptide samples
See selection chart on page 33 for assitance selecting the
correct product for your application
SPECIFICATIONS:
1-10 µL ProteaTips:
• Binding capacity:
• Amount of TiO2 beads in a tip:
• Sample volume:
10-200 µL ProteaTips:
• Binding capacity:
• Amount of TiO2 beads in a tip:
• Sample volume:
MALDI-TOF spectrum of a 0.2 µg β-casein digest
unprocessed (A) and processed using a TiO2 Proteatip (B),
showing phosphopeptide enrichment for the sample prepped
with the titanium dioxide tips. The colored arrows in (B)
indicate enriched phosphopeptides
ORDERING INFORMATION:
1-2 µg
30 µg
1-10 µL
2-4 µg
75 µg
10-200 µL
TiO2 ProteaTip Sample Prep Kit
1-10 µL Tips. Kit contains Titanium Dioxide ProteaTips, Sample
Reconstitution and Wash #1 Solution, Wash #2 Solution, and
Elution Solution.
Catalog NumberSizePrice
SP-124-24
24 Tips
$89
SP-124-96
96 Tips
$289
TiO2 ProteaTip Sample Prep Kit
10-200 µL Tips. Kit contains Titanium Dioxide ProteaTips,
Sample Reconstitution and Wash #1 Solution, Wash #2 Solution,
and Elution Solution.
Catalog NumberSizePrice
SP-125-24
24 Tips
$100
SP-125-96
96 Tips
$310
www.proteabio.com • 26
Protein Sample Preparation
TiO2 SpinTips Sample Prep Kit
The TiO2 SpinTips Sample Prep Kit contains higher capacity tips
and reagents for enrichment of phosphopeptides from complex
peptide samples. The newly formulated reagent solutions
supplied with Protea’s TiO2 Sample Prep Kits are specially
formulated to maximize the retention of phosphorylated
peptides, while simultaneously minimizing the retention of nonphosphorylated peptides..
The SP-154-24 kit contains 24 TiO2 SpinTips for 50-1000 µL
sample volumes, Sample Reconstitution and Wash #1 Solution,
Wash #2 Solution, and Elution Solution.
The SP-154-96 kit contains 96 TiO2 SpinTips for 50-1000 µL
sample volumes, Sample Reconstitution and Wash #1 Solution,
Wash #2 solution, and Elution Solution.
FEATURES:
•
•
High throughput sample preparation applications
Greater binding capacity: 1000 µg/tip (50-1000 µL sample
solution)
RESEARCH APPLICATIONS:
•
•
•
Selective enrichment of phosphopeptides from
in-gel digested proteins for identification of protein
phosphorylation sites by mass spectrometry
Selective enrichment of phosphopeptides from complex
protein/peptide samples
See selection chart on page 33 for assitance selecting the
correct product for your application.
ORDERING INFORMATION:
TiO2 SpinTips Sample Prep Kit
Kit contains Titanium Dioxide SpinTips, Sample Reconstitution
and Wash #1 Solution, Wash #2 Solution, and Elution Solution.
Catalog NumberSizePrice
SP-154-24
SP-154-96
24 tips
96 tips
$163
$499
MALDI-TOF MS spectra of 1 pmol protein digest mixture of
bovine serum albumin (BSA), ovalbumin, and transferrin, spiked
with 1 pmol of Protea’s Phosphopeptide Standard I (PS-180).
The spectra compare the re-formulated TiO2 SpinTip Sample
Prep Kit (A) with the old formulation TiO2 SpinTip Sample Prep
Kit (B). Spectrum (C) is the same sample processed with C18
SpinTip Sample Prep Kit (SP-150) for selective phosphopeptide
enrichment. The green arrow denotes the phosphopeptide
standards with their respective m/z: angiotensin II (1126),
cholecystokinin (1331), and calcitonin (1801). There is
a significant improvement of the S/N ratio and selective
enrichment for the phosphopeptides in (A).
27 • 877.776.8321
SCX SpinTips Sample Prep Kit
The Strong Cation Exchange (SCX) SpinTips Sample Prep
Kit contains tips and reagents for strong cationic exchange
solid phase extraction of protein and peptide samples for
electrospray or MALDI mass spectrometry. The kit is effective in
concentrating, desalting, and removal of detergent from protein
and peptide samples, resulting in mass spectra with improved
S/N and reduced salt adducts. SCX SpinTips can also be used
for sample fractionation in off-line MudPIT experiments.
The SP-155-24 kit contains 24 SCX SpinTips for 50-1000 µL
volumes, Sample Reconstitution Solution, Wash Solution, and
Elution Solution.
ORDERING INFORMATION:
The SP-155-96 kit contains 96 SCX SpinTips for 50-1000 µL
volumes, Sample Reconstitution Solution, Wash Solution, and
Elution Solution.
SCX SpinTips Sample Prep Kit
Kit contains Strong Cation Exchange (SCX) SpinTips, Sample
Reconstitution Solution, Wash Solution, and Elution Solution.
FEATURES:
•
•
Catalog NumberSizePrice
High throughput sample preparation applications
Greater binding capacity: 1000 µg/tip (50-1000 µL sample
solution)
SP-155-24
SP-155-96
24 tips
96 tips
$142
$446
RESEARCH APPLICATIONS:
1320
1316.6
1858.9
1 28.6
1740
2160
Mass (m/z)
3000
2580
D
228 .1
0900
23 1.1
1892.8
1320
19 9.9
1627.7
1676.7
1 91.6
1333.6
1360.7
1225.6
3000
1740
2160
Mass (m/z)
22 8.9
100
2580
3000
F
www.proteabio.com • 28
3000
0900
1320
1740
2160
Mass (m/z)
2585.0
2305.9
2161.0
1936.9
1993.9
2032.9
1633.5
1690.5
1755.8
1296.6
2580
1 39.7
1 91.6
15 8.6
% Intensity
2032.9
2160
Mass (m/z)
993.3
1050.
2580
2191.1
22 8.9
2306.9
1633.5
1740
1168.6
% Intensity
2736.2
2637.2
23 1.1
2529.2
2198.2
1858.9
1892.9
2160
Mass (m/z)
E
15 8.6
1390.6
1168.5
1225.6
% Intensity
C
228 .1
1 28.6
1360.7
1 79.7
1740
1333.6
100
109 .
1320
3000
2735.2
2580
B
2377.0
2 35.0
1859.9
2112.9
0
900
2160
Mass (m/z)
1 91.6
1320
% Intensity
10 5.5
1801.8
A
175 .8
1740
927.
0900
0900
100
100
1316.6
1102.5
% Intensity
100
1672.7
1320
10 5.5
900
993.3
0
1567.7
102 .3
1 39.6
% Intensity
100
1687.8
1753.8
Detergent removal from protein and peptide samples
Effective desalting of proteins and peptides
Sample fractionation in MuDPIT experiments
See selection chart on page 33 for assitance selecting the
correct product for your application.
1159.5
•
•
•
•
2580
3000
MALDI-TOF mass spectra
from fractionation of a 70 µg
digest mixture of 7 proteins
(cytochrome C, myoglobin,
lysozyme, α-lactalbumin,
carbonic anhydrase,
ovalbumin and bovine
serum albumin) using a
SCX SpinTip. Panels A to
F correspond to stepwise
elution of the peptides from
SCX SpinTip using 20, 60,
100, 150, 250 and 400
mM ammonium formate
in 20% acetonitrile, pH 3
respectively.
Protein Sample Preparation
C8 GPR SpinTips Sample Prep Kit for MALDI
ORDERING INFORMATION:
C8 GPR SpinTips Sample Prep Kit for MALDI
Kit contains C8 SpinTips, Sample Reconstitution and Rinse
Solution, GPR Rinse Buffer for MALDI, Equilibration Solution, and
Elution Solution.
Catalog NumberSizePrice
SP-171-24
SP-171-96
24 tips
96 tips
$131
$394
C8 GPR SpinTips Sample Prep Kit for ESI
ORDERING INFORMATION:
C8 GPR SpinTips Sample Prep Kit for ESI
Kit contains C8 SpinTips, Sample Reconstitution and Rinse
Solution, GPR Rinse Buffer for ESI, Equilibration Solution, and
Elution Solution.
Catalog NumberSizePrice
SP-161-24
24 tips
$131
SP-161-96
96 tips
$394
Sample Prep FAQ:
Q: Does centrifugation of samples for longer times or higher centrifuge speeds add any benefit using your Protea SpinTip products?
A: All of our SpinTip products (SP-150, SP-151, SP-152, SP-155, SP-154, SP-161 and SP-171) have fritted tips. The SpinTip
protocols recommend centrifugation at 4,000 rpm for 3 minutes. However, laboratory centrifuges may differ and different samples
may take longer for the entire sample to pass through the chromatographic material.
29 • 877.776.8321
C18 LithTips Sample Prep Kit
The C18 LithTips Sample Prep Kits contain tips and reagents
for reversed-phase solid phase extraction for protein sample
clean-up and for spotting of peptides and small proteins from
samples for MALDI mass spectrometry. These kits are effective in
concentrating and desalting peptides and small proteins, resulting
in mass spectra with better S/N and reduced salt adducts. The
Sample Prep Kits contain C18 LithTips for 1-10 µL sample volumes,
Sample Reconstitution and Rinse solution, Equilibration Solution,
and Elution Solution. The C18 monoliths are immobilized on the
inner surface of a pipette tip, providing a large surface area for
sample capture. In addition, the central portion of the monolith is
hollow, eliminating backpressure problems during pipetting.
FEATURES:
•
•
•
Effective desalting of protein samples
Low backpressure during sample processing
Binding Capacity: 0.5 µg
ORDERING INFORMATION:
C18 LithTips Sample Prep Kit
RESEARCH APPLICATIONS:
•
•
•
•
Kit contains C18 LithTips, Reconstitution and Rinse Solution,
Equilibration Solution, and Elution Solution.
Catalog NumberSizePrice
SP-400-24
24 tips
$60
SP-400-96
96 tips
$195
Desalting peptides from protein digests prior to analysis by
mass spectrometry
MALDI spotting of peptide and small protein samples
Concentration of peptides extracted from dilute solutions
See selection chart on page 33 for assitance selecting the
correct product for your application.
1480.0
Mass (m/z)
3512.1
2524.4
1881.2
2.2 E+4
1890.1
1741.1
1640.1
1567.9
1119.6
0
900
1419.9
927.6
% Intensity
100
1440.0
4000
MALDI peptide mass fingerprint of 1 pmol of a BSA tryptic digest spotted with a C18 LithTip on
a MALDI target pre-spotted with CHCA matrix and analyzed by an ABI 4800 TOF/TOF mass
spectrometer.
www.proteabio.com • 30
Protein Sample Preparation
C8 LithTips Sample Prep Kit
The C8 LithTips Sample Prep Kits contain tips and reagents for
reversed-phase solid phase extraction for protein sample cleanup and for spotting of small and medium-size proteins for MALDI
mass spectrometry. These kits are effective in concentrating
and desalting small and large proteins, resulting in mass spectra
with better S/N and reduced salt adducts. The Sample Prep
Kits contains C8 LithTips for 1-10 µL sample volumes, Sample
Reconstitution and Rinse solution, Equilibration Solution, and
Elution Solution. The C8 monoliths are immobilized on the inner
surface of a pipette tip, providing a large surface area for sample
capture. In addition, the central portion of the monolith is hollow,
eliminating backpressure problems during pipetting.
FEATURES:
•
•
•
ORDERING INFORMATION:
Effective desalting of small and medium-sized protein
samples
Low backpressure during sample processing
Binding Capacity: 0.5 µg
C8 LithTips Sample Prep Kit
Kit contains C8 LithTips, Reconstitution and Rinse Solution,
Equilibration Solution, and Elution Solution.
Catalog NumberSizePrice
SP-420-24
24 tips
$60
SP-420-96
96 tips
$195
RESEARCH APPLICATIONS:
•
•
•
•
•
MALDI spotting of small and medium-size proteins
Desalting proteins from cell lysates
Concentration of proteins extracted from dilute solutions
Clean-up of proteins electroeluted from polyacrylamide gels
See selection chart on page 33 for assitance selecting the
correct product for your application.
14185.5
100
3.0 E+4
% Intensity
12363.3
6176.0
16969.1
7085.1
8477.5
0
4000
11000
18000
25000
32000
Mass (m/z)
MALDI-TOF mass spectrum of a 3 protein mixture (20 pmol each protein) spotted with a C8
LithTip onto a MALDI target pre-spotted with CHCA matrix: cytochrome C [m/z = 12363.3 (+1),
m/z = 6176.0 (+2)], α-lactalbumin [m/z = 14185.5 (+1), m/z = 7085.1 (+2)], and myoglobin [m/z =
16969.1 (+1), m/z = 8477.5 (+2)].
31 • 877.776.8321
C4 LithTips Sample Prep Kit
The C 4 LithTips Sample Prep Kits contain tips and reagents for
reversed-phase solid phase extraction for protein sample cleanup and for spotting of medium and large-size proteins for MALDI
mass spectrometry. These kits are effective in concentrating and
desalting medium and large proteins, resulting in mass spectra
with better S/N and reduced salt adducts. The Sample Prep
Kits contain C 4 LithTips for 1-10 µL sample volumes, Sample
Reconstitution and Rinse solution, Equilibration Solution, and
Elution Solution. The C 4 monoliths are immobilized on the inner
surface of a pipette tip, providing a large surface area for sample
capture. In addition, the central portion of the monolith lining the
tip is hollow, eliminating backpressure problems during pipetting.
FEATURES:
•
•
•
Effective desalting of protein samples
Low backpressure during sample processing
Binding Capacity: 0.5 µg
ORDERING INFORMATION:
C4 LithTips Sample Prep Kit
RESEARCH APPLICATIONS:
•
•
•
•
•
MALDI spotting of medium and large-size proteins
Desalting proteins from cell lysates
Concentration of proteins extracted from dilute solutions
Clean-up of proteins electroeluted from polyacrylamide gels
See selection chart on page 33 for assitance selecting the
correct product for your application.
Kit contains C 4 LithTips, Sample Reconstitution Solution,
Equilibration Solution, and Elution Solution.
Catalog NumberSizePrice
SP-410-24
24 tips
$60
SP-410-96
96 tips
$195
33276.3
% Intensity
100
5265.6
66510.7
22198.5
0
20000
32000
44000
56000
68000
80000
Mass (m/z)
MALDI-TOF mass spectrum of a 20 pmol bovine serum albumin (BSA) sample spotted with a
C 4 LithTip onto a MALDI target pre-spotted with CHCA matrix: m/z = 66510.7 (+1), m/z = 33276.3
(+2) and m/z = 22198.5 (+3).
www.proteabio.com • 32
Application
ZIC® -HILIC
ProteaTips
Salt Removal
X
Detergent
Removal
X
Sample
Concentration
X
TiO2
ProteaTips,
SpinTips
X
Cation
Exchange Solid
Phase Extration
C18
ProteaTips,
SpinTips
C8
SpinTips
C4
SpinTips
C18
LithTips
C8
LithTips
C4
LithTips
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
SCX
SpinTips
X
Reversed Phase
Solid Phase
Extraction
Enriches
Peptides
Complementary
to Reverse
Phase
Extraction
X
Phosphopeptide
Enrichment
X
Good for
Peptides and
Small Proteins*
X
X
X
Good for
Medium-sized
Proteins*
X
X
Good for
Medium and
Large Proteins*
X
X
Monolith
Technology
Can Handle
Large Samples
X (SpinTip)
X
X (SpinTip)
X
X
X
X
X
X
X
X
X
MALDI spotting
from tip
X
X (ProteaTips)
MALDI-MS
Compatible
X
X
X
X
X
X
X
X
X
ESI-MS
Compatible
X
X
X
X
X
X
X
X
X
SP-120-24
SP-120-96
SP-121-24
SP-121-96
SP-124-24
SP-124-96
SP-125-24
SP-125-96
SP-154-24
SP-154-96
SP-155-24
SP-155-96
SP-129-24
SP-129-96
SP-150-24
SP-150-96
SP-151-24
SP-151-96
SP-152-24
SP-152-96
SP-400-24
SP-400-96
SP-420-24
SP-420-96
SP-410-24
SP-410-96
Catalog
Numbers
X (ProteaTip)
*Peptides: 1-5kDa Small Proteins: 5-30kDa Medium Proteins: 30-100kDa Large Proteins: >100kDa
33 • 877.776.8321
Protein Sample Preparation
Functionalized Tip Selection Chart
C18 LithColumns
C18 LithColumns are robust, reproducible monolithic
chromatography columns for reversed phase HPLC and LC-MS
separations of peptides and small proteins. These polyimide
coated fused silica capillary columns contain porous organic
polymer monoliths that are polymerized in situ using the
highest quality reagents and materials available, following rigid
formulation and production protocols.
By optimizing the monolith formulation and production conditions,
LithColumns balance high quality separation requirements
with lower column flow resistance and higher flow rates. The
polymeric monolith chemistry permits operation at a wider pH
range (2–10) than traditional silica-based columns, both beadbased and monolithic. LithColumns are fabricated inside standard
360 µm O.D. (100 µm I.D.) and 655 O.D. (250 µm I.D.) fused silica
capillary, and they can be quickly and easily inserted into any
plumbing scheme with conventional fittings and adapters.
SPECIFICATIONS:
Flow Rate:
•
•
0.5 – 5.0 µL/min (100 µm ID)
1.0 – 10.0 µL/min (250 µm ID)
Reversed phase LithColumns are available in C 4, C8 and C18
chemistries, in 100 µm and 250 µm inner diameters, and in 10
cm and 20 cm lengths. Each lot is batch tested for performance
on LC-MS and for structural evaluation by scanning electron
microscopy (SEM) for quality assurance.
•
500 – 5000 psi
•
•
0.1 µg (100 µm ID)
1.0 µg (250 µm ID)
FEATURES:
C18 LithColumns, 360/100 µm O.D./I.D., 10 cm
•
•
•
•
Wide range of operational pH ranges (pH 2 to 10)
Low column backpressures
Reproducible and efficient separations
Rapid separations with higher flow rates
RESEARCH APPLICATIONS:
•
•
•
LC-MS and LC-MALDI analysis of peptides and protein
digests
Peptide mapping studies
LC-MS analysis of small molecules
Backpressure:
Sample Capacity:
ORDERING INFORMATION:
Catalog NumberSizePrice
LC-100-1 LC-100-3 1 column
3 columns
$495
$1290
C18 LithColumns, 360/100 µm O.D./I.D., 20 cm
Catalog NumberSizePrice
LC-101-1 LC-101-3 1 column
3 columns
$695
$1810
C18 LithColumns, 655/250 µm O.D./I.D., 10 cm
Catalog NumberSizePrice
LC-110-1 LC-110-3 1 column
3 columns
$495
$1290
C18 LithColumns, 655/250 µm O.D./I.D., 20 cm
Catalog NumberSizePrice
LC-111-1 LC-111-3 1 column
3 columns
$695
$1810
LC Chromatogram of angiotensin 1 and
somatostatin peptides on a 10 cm, 100 µm I.D>
monolithic capillary column. The separation was
performed at 0.5 µL/min for 20 min, with 12.5 pmol
of each peptide injected in a 10 µL volume. Mobile
phase A was 0.1% TFA in 2% acetonitrile and
mobile phase B was 0.1 % TFA in 98 % acetonitrile.
The separation was performed using an ABI
TempoTM LC system with UV detection at 214 nm.
www.proteabio.com • 34
Protein Sample Preparation
C4 and C8 LithColumns
C 4 and C8 LithColumns are robust, reproducible monolithic
chromatography columns for reversed phase HPLC and LCMS separations of small to intermediate-size proteins. These
polyimide coated fused silica capillary columns contain porous
organic polymer monoliths that are polymerized in situ using the
highest quality reagents and materials available, following rigid
formulation and production protocols.
By optimizing the monolith formulation and production conditions,
LithColumns balance high quality separation requirements
with lower column flow resistance and higher flow rates. The
polymeric monolith chemistry permits operation at a wider pH
range (2 – 10) than traditional silica-based columns, both
bead-based and monolithic. LithColumns are fabricated inside
standard 360 µm O.D. (100 µm I.D.) and 655 O.D. (250 µm I.D.)
fused silica capillary, and they can be quickly and easily inserted
into any plumbing scheme with conventional fittings and adapters.
ORDERING INFORMATION:
C8 LithColumns, 360/100 µm O.D./I.D., 10 cm
Reversed phase LithColumns are available in C 4, C8 and C18
chemistries, in 100 µm and 250 µm inner diameters, and in 10
cm and 20 cm lengths. Each lot is batch tested for performance
on LC-MS and for structural evaluation by scanning electron
microscopy (SEM) for quality assurance.
Catalog NumberSizePrice
FEATURES:
LC-121-1 LC-121-3 •
•
•
•
Organic polymer monolith available in 100 and 250 µm I.D.
options
Wide range of pH working conditions (pH 2 to 10)
Low column flow resistance
Available in 10 cm and 20 cm lengths
RESEARCH APPLICATIONS:
•
•
LC-MS and LC-MALDI analysis of small to intermediate-size
proteins
LC-MS analysis of peptides and protein digests
SPECIFICATIONS:
Flow Rate:
•
•
0.5 – 5.0 µL/min (100 µm ID)
1.0 – 10.0 µL/min (250 µm ID)
•
500 – 5000 psi
•
•
0.25 µg (100 µm ID)
1.5 µg (250 µm ID)
Backpressure:
Sample Capacity:
LC-120-1 LC-120-3 1 column
3 columns
$495
$1290
C8 LithColumns, 360/100 µm O.D./I.D., 20 cm
Catalog NumberSizePrice
1 column
3 columns
$695
$1810
C8 LithColumns, 655/250 µm O.D./I.D., 10 cm
Catalog NumberSizePrice
LC-130-1 LC-130-3 1 column
3 columns
$495
$1290
C8 LithColumns, 655/250 µm O.D./I.D., 20 cm
Catalog NumberSizePrice
LC-131-1 LC-131-3 1 column
3 columns
$695
$1810
C4 LithColumns, 360/100 µm O.D./I.D., 10 cm
Catalog NumberSizePrice
LC-140-1 LC-140-3 1 column
3 columns
$495
$1295
C4 LithColumns, 360/100 µm O.D./I.D., 10 cm
Catalog NumberSizePrice
LC-141-1 LC-141-3 1 column
3 columns
$695
$1795
C4 LithColumns, 655/250 µm O.D./I.D., 10 cm,
with Fittings
Catalog NumberSizePrice
LC-150-1 LC-150-3 1 column
3 columns
$495
$1290
C4 LithColumns, 655/250 µm O.D./I.D., 20 cm,
with Fittings
Catalog NumberSizePrice
LC-151-1 LC-151-3 1 column
3 columns
$495
$1795
35 • 877.776.8321
© 2011 Protea Biosciences, Inc • 877.776.8321 • www.proteabio.com • LN0101111
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