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How to contact us:
Where to Order Contact any authorized J.T.Baker distributor
or Mallinckrodt Baker +31-570-687-500
Information
and Assistance
Call +31-570-687-500 for Customer Service and Assistance
Internet
Address
http://www.jtbaker.com
EUROPEAN HEADQUARTERS:
Mallinckrodt Baker B.V.
Teugseweg 20, P.O. Box 1, 7400 AA, Deventer, The Netherlands
Tel: +31-570-687500; Fax: +31-570-687574
E-mail: [email protected]
FRANCE: Mallinckrodt Baker, France
33 Avenue Philippe Auguste, 75011, Paris, France
Tel: +33-1-44-64-89-71/2; Fax: +33-1-44-64-89-73
E-mail: [email protected]
GERMANY: Mallinckrodt Baker, Deutschland
Im Leuschnerpark 4, 64347 Griesheim, Germany
Tel: +49-6155-667939; Fax: +49-6155-667940
E-mail: [email protected]
ITALY: Mallinckrodt Baker, Italia
Via P. Portaluppi 11/2, 20138 Milano, Italy
Tel: +39-02-580891; Fax: +39-02-58019264
E-mail: [email protected]
Sample
Preparation
UNITED KINGDOM: Mallinckrodt Baker, UK
107/112 Leadenhall Street, London EC3A 4AH, United Kingdom
Tel: +44-1908-506000; Fax: +44-1908-503290
E-mail: [email protected]
NORTH AMERICA: Mallinckrodt Baker, Inc.
222 Red School Lane, Philipsburg, NJ 08865, USA
Tel: 800-582-2537 within U.S.; 908-859-2151
E-mail: via www.solvitcenter.com
MEXICO: Mallinckrodt Baker S.A. de C.V.
Col. Lindavista, Deleg. Gustavo A. Madero, 07300 Mexico D.F., MEXICO
Tel: 52-555-569-1100 Fax: 52-555-755-2585
E-mail:[email protected]
When every
step counts
MALAYSIA: Mallinckrodt Baker Sdn. Bhd.
26 Jalan SS26/13, 47301 Petaling Yaha, Selangor, MALAYSIA
Tel: (603) 78030378 Fax: (603) 78030405
E-mail: [email protected]
SINGAPORE: Mallinckrodt Baker, Inc. c/o Tyco Healthcare Pte. Ltd.
No. 26, Ang Mo Kio Industrial Park 2, #04-01, 569507 SINGAPORE
Tel: 656-4820 100 Fax: 656-4820-300
BAKERBOND™, Speedisk™, ULTRA RESI-ANALYZED™,
BAKER ANALYZED™, CYCLE-TAINER™, the J.T.Baker logo
and J.T.Baker® are all trademarks of Mallinckrodt Baker, Inc.
C-10/10.000 0104
www.jtbaker.com
J.T.Baker
Your professional guide through Solid Phase Extraction
Introduction
Among chromatography suppliers,
J.T.Baker stands out with a unique
breadth of separations capabilities.
You may know us for our high purity
reagents, but we have also been in
the Liquid Chromatography (LC)
business since the 1970s. In fact, we
pioneered the development of Solid
Phase Extraction (SPE).
We’re manufacturers of chromatographic media, developers of
proprietary bonding chemistry, and
providers of products and know-how
for diverse separations techniques,
scale and applications. Our products
for analytical separations are focused
in three distinct applications:
mated liquid handling systems, is
the most recent addition to the
Speedisk family. Our Speedisk
columns perform 9 times faster
than conventional SPE columns.
Solid Phase Extraction for
Sample Preparation
We are never satisfied with the
status quo. Our improved BAKERBOND spe products provide standard,
reliable performance for today’s
research, as well as QC pharmaceutical, clinical and environmental
applications. Our extraction tube
design is optimized for SPE, and
compatible with manual and automated SPE column processors. Our
proprietary column component
formulations are ultraclean.
The purpose of
sample preparation
Sample clean up
Necessary to eliminate impurities
and/or isolate the component of
interest from the matrix. This helps
to increase lifetime of the analytical
column, prevents contamination of
equipment, thereby protecting
expensive instrumentation.
Sample concentration
in order to reach the detection
limits of the analytical equipment.
Sample preparation selectively
concentrates the components of
interest prior to analysis.
Product characteristics
• Analytical HPLC - With
BAKERBOND™ media for large
molecule or small molecule
separations available in columns
or as bulk media.
• Flash Media and Cartridges Our new, high-performance
spherical silica media can be
functionalized with a variety of
surface chemistries for optimal
application performance.
• Solid Phase Extraction
(both BAKERBOND
conventional columns
and Speedisk™ high
performance columns)
- Our new 96-Well
plate, optimal for auto-
when standard speed, recovery, and
final concentration with good
economy are needed.
• Optimized, round-rimmed tube
and frits made from ultrapure
polymers.
• Tubes fit into all popular
processors and racks.
• Multi phase columns for special
environmental applications (e.g.
PAH’s, PCB’s and Pesticides)
• Mixed Mode narc™ columns for
drugs of abuse sample preparation
• Wide Pore (300 Å) sorbents for
biological applications.
BAKERBOND Speedisk columns
Screen
capacity, optimum recovery and
more effective separation. The design
is especially useful in clinical, biological
and pharmaceutical drug applications.
Speedisk columns are disposable,
ultraclean, polypropylene columns
(1-6 ml), pre-packed with high
performance micro particles of 10µm
(10-200 mg) and available in a wide
range of sorbents (20 silica based
and 6 polymer based) to meet all
your separation needs.
‘Method Development Kits’ are
available for BAKERBOND spe and
Speedisk columns. Please contact your
local office for more information.
Speedisk 96 Columns and
Speedisk 96-well plates
Guard Filter
Furthermore, a wide
range of BAKERBOND
sorbents let you
rationally choose the
functionality that’s
appropriate for your
sample.
BAKERBOND spe Columns
Microparticulate Sorbent
Support Filter
Screen
BAKER spe- 12G Column Processor
With BAKERBOND spe columns,
you can choose the solid phase
extraction column that best fits
your sample size and performance
requirements. Use standard
BAKERBOND spe 1, 3 and 6 ml
ultraclean polypropylene and glass
columns, packed with 50-3000 mg
sorbent, for samples from 0.2 to 10 ml
J.T.Baker Speedisk columns reduce
SPE run times by 60-80% and may
eliminate other time consuming
sample preparation steps, such as
pre-filtration and evaporation, sometimes required with conventional SPE
columns.
Thanks to the unique patented
Speedisk laminar design and
BAKERBOND high performance
micro-particles you not only get
shorter run times, but also higher
Speedisk 96 columns multiply the
advantages of Speedisk columns
by 96 to raise high throughput SPE
to a higher level of performance.
We offer these Speedisk products in
two different geometries. For method
development we offer the versatile
modular Speedisk 96 columns. We
insert 96 “rimless” 20 mg Speedisk
columns in the Speedisk 96 column
holder and the assembly is ready for
placement on the Speedisk 96
Positive Pressure Processor.
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography
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3
Fast, innovative & reliable
Sample preparation with J.T.Baker SPE technology
You can remove unneeded columns
from the holder, and assemble custom
trays for efficient method development. Once method development is
complete, use the Speedisk 96-well
plate, a one-piece, molded plate that
is pre-assembled with the sorbent of
your choice. The new Speedisk 96-well
plate was designed with standard
geometries to adapt to popular automated liquid handling systems.
Speedisk 48 Positive Pressure Processor:
The Speedisk Positive Pressure
Processor is perfect for processing 1,
3 and 6 ml columns in batches of
1-48 samples. It distributes N2 or
compressed air to displace liquid
from BAKERBOND spe columns
or BAKERBOND Speedisk columns
and it easily enables a high control
and precision.
Accessories for Extraction Columns
Choose from more than 20 functionalities of silica and polymer-based
sorbents for either Speedisk 96 columns
or the new Speedisk 96-well plates.
Positive Pressure Processors and
Accessories for Extraction Columns
Comparison between BAKERBOND spe columns and BAKERBOND
Speedisk columns:
filtration capacity so it can easily
handle samples with high particulates.
The BAKERBOND Speedisk design
resists clogging and ensures high
throughput rates even when samples
contain solids. Flow rate, capacity,
recovery, and precision are high due
to the unique disk configuration
and performance of BAKERBOND
sorbent.
BAKERBOND
spe column
BAKER spe- 12G Column Processor
Speedisk 48 and 96 Positive Pressure Processor and
Speedisk 96 Column Holder
Speedisk 96 Positive Pressure Processor:
The Speedisk Positive Pressure
Processor enables you to combine
the advantages of a positive displacement processor with Speedisk columns.
The processor produces the same
flow rate in each of the 96 columns
arrayed in 96 well plate.
Mallinckrodt Baker also offers a wide
range of accessories for your separation
technology. Please go to our website,
www.jtbaker.com/chromatography and
select accessories.
BAKERBOND Speedisk
Extraction Disks
The correct choice for water samples
from 200 ml to 2 L. Our patented
disk is pre-assembled for use in
preparing aqueous samples for analysis.
Its laminar configuration provides
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography
4
BAKERBOND
Speedisk column
BAKERBOND
Sample preparation step
BAKERBOND spe columns
BAKERBOND Speedisk columns
Column Size / Sorbent
1 cc / 100 mg
1 cc / 20 mg
Particle Size
40 µm
10 µm
Sample Volume
2 ml
1 ml
Column conditioning
2 ml (20-40 sec)
0.5 ml (5-10 sec)
Sample addition
2 ml (100 sec)
50 ul-0,5 ml (50 sec)
Washing
1.5 ml (15-20 sec)
0.4 ml (2-5 sec)
Elution
1-2 ml (15-20 sec)
0,3-0,6 ml (5-10 sec)
Sample concentration / evaporation
3-10 minutes
reduced or eliminated
General product overview
Silica based
SPE products
Polymer based
SPE products
• BAKERBOND spe
columns:
reverse phase
normal phase
ion exchange
adsorption
drug of abuse
• Styrene Divinylbenzene
copolymer (SDB)
BAKERBOND spe columns.
• BAKERBOND Speedisk
columns:
reverse phase
normal phase
ion exchange
adsorption
drug of abuse
• Speedisk Divinylbenzene
(DVB) columns
H2O-Philic DVB
H2O-Philic SC-DVB
H2O-Phobic DVB
H2O-Phobic SC-DVB
H2O-Philic SA-DVB
H2O-Phobic WA-DVB
• BAKERBOND Speedisk 96
Silica Columns (in holder)
• BAKERBOND Speedisk 96
Polymer Columns (in holder)
Speedisk 96
well plates
BAKERBOND
Speedisk
extraction disk
• BAKERBOND Speedisk
96-Well Plates Polymer
Sorbents (compatible with
automatic systems)
• BAKERBOND Speedisk
extraction disks:
for manual extraction stations
and for automated extractors.
• BAKERBOND Speedisk
extraction disks:
for manual extraction stations
and for automated extractors
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J.T.Baker
Your professional guide through Solid Phase Extraction
Selection Guide for spe sorbents and solvents
ORGANIC SAMPLES MW< 2000 (in solution)
This Sorbent Selection Guide is a systematic guide classifying samples according to polarity, ionizability and solubility in water or organic solvents. This information is useful in
selecting the necessary components of a preliminary extraction method
Organic Solvent Soluble
Sample
Solubility
Water Soluble
Ionic
Matrix
sample
Organic
Organic
Aqueous
Polar
Moderately Polar
Non Polar
Anionic
NPC
LSC
RPC
SPE Phase
recomm.2
H2O-Philic DVB
Cyano
Diol
Amino
1,2 Amino
H2O-Phobic DVB
H2O-Philic DVB
Silica gel
Florisil
Alumina
Solvents3,4
Hexane
Chloroform
Dichloromethane
Acetone
Methanol
Hexane
Chloroform
Dichloromethane
Ethyl acetate
Methanol
Separation
Mechanism1
Aqueous
Aqueous
Aqueous
Cationic
Non Polar
Moderately
Polar
Polar
IEC
IEC
RPC
LSC
NPC
H2O-Phobic DVB
H2O-Philic DVB
SDB-1/SDB-2
Octadecyl
Octyl
Cyclohexyl
Phenyl
Cyano
H2O-Phobic
H2O-Philic DVB
Amino
1,2 Amino
Quaternary Amine
H2O-Phobic SA-DVB H2O-Phobic SC-DVB H2O-Phobic DVB
H2O-Philic SA-DVB H2O-Philic SC-DVB H2O-Philic DVB
Cyano
SDB-1/SDB-2
Silicag gel
Carboxylic Acid
Octadecyl
Florisil
Sulfonic Acid
Octyl
Alumina
Cyclohexyl
Phenyl
Cyano
H2O-Philic DVB
Cyano
Diol
Amino
1,2 Amino
Hexane
Dichloromethane
Acetone
Acetonitrile
Methanol
Water
Acids, buffers
Acid, bases, buffers Hexane
Dichloromethane
Acetone
Acetonitrile
Hexane
Chloroform
Dichloromethane
Acetone
Methanol Methanol
1
Separation Mechanisms
LSC: Liquid Solid Chromatography (Adsorption)
NPC: Normal Phase Chromatography (Bonded Phase Partition)
RPC: Reversed Phase Chromatography (Bonded Phase Partition)
IEC: Ion-Exchange Chromatography (Bonded Phase Ion-Exchange)
SDB: styrene divinyl benzene
DVB: divinyl benzene polymer
H2O-Phobic WA DVB: Weak anion exchanger
H2O-Phobic SC DVB: Strong cation exchanger
H2O-Philic SA DVB Strong anion exchanger
H2O-Philic SC DVB: Strong cation exchanger
6
Non-ionic/Ion-paired
Aqueous
Hexane
Chloroform
Dichloromethane
Ethyl acetate
Methanol
Water
For trouble free analysis, we recommend the following product lines and
product information out of the J.T.Baker programme:
• ULTRA RESI-ANALYZED solvents
• BAKER ANALYZED HPLC-Reagents solvents
• BAKER ANALYZED LC-MS reagents
• BAKERBOND HPLC columns for various applications
• BAKERBOND Speedisk Column Instructions
• Solid Phase Extraction - Technical guide
• CYCLE-TAINER™ solvent delivery system
• Cross reference lists available on the website
• Application Notes
2
Bonded phases listed in order of increasing polarity
Eluting solvents listed in order of increasing polarity
4
Selective elution can be performed by combining two or more miscible solvents
to achieve various degrees of polarity
3
3,4
Solvents:
9262 Hexanes (95% n-hexane), ULTRA RESI-ANALYZED
9257 Chloroform, ULTRA RESI-ANALYZED
9264 Dichloromethane, ULTRA RESI-ANALYZED
9260 Ethyl acetate, ULTRA RESI-ANALYZED
9254 Acetone, ULTRA RESI-ANALYZED
9255 Acetonitrile, ULTRA RESI-ANALYZED
9077 Methanol , ULTRA RESI-ANALYZED
4219 Water, ULTRA RESI-ANALYZED
If your free sample isn’t
here, please contact
our customer service
department
This Sorbent Selection Guide is also available on:
For detailed information on our separation programme please visit
www.jtbaker.com/chromatography/SolidPhaseExtraction.asp
www.jtbaker.com/chromatography
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