1. pets
2. cats

Sample Collection and
Analysis of Carbonyls
Improve Detection of Trace Level
Contaminants in Air
Sample Collection Devices
Analytical Columns for
Sample Analysis
Air Monitoring Standards
Applications
Improve Sampling and Detection of Carbonyls in Air
Air Sampling Products
Leverage a Wide Range of Samplers and Accessories
Achieve sensitive and reliable results with air sampling media for
a wide range of applications in various configurations for solvent
desorption, thermal desorption, passive and whole air sampling
from Supelco. Ensure low background for carbonyl sampling
with products that are produced to the highest quality standards
in a carbonyl controlled manufacturing environment that are
complemented by high-purity solvents and calibration standards
from Fluka and RTC.
The sampling of aldehydes and ketones requires an on-site
derivatization on a coated adsorbent e.g. with dinitrophenylhydrazine (DNPH). Supelco's
product range includes the low
back pressure DNPH (LpDNPH)
cartridges (available in 6 different
configurations and 16 products)
for active sampling, which ensure
longer pump operation, glass
tubes (ORBO) and DNPH coated filters as well as devices with
otherwise coated adsorbents for sampling carbonyls.
Our product range is suitable for OSHA, NIOSH, ASTM, EPA and
CARB methods. For a complete overview on air monitoring from
Sigma-Aldrich, visit sigma-aldrich.com/air-monitoring.
LpDNPH Products
LpDNPH cartridges are air sampling devices designated for
sampling carbonyls (e.g. formaldehyde) in ambient, indoor and
industrial atmospheres. Carbonyls are trapped on a high-purity
adsorbent coated with 2,4-dinitrophenylhydrazine where they
are converted to hydrazone derivatives. The derivatives are eluted
from the cartridge with acetonitrile and analyzed by HPLC, in
most cases. Select LpDNPH products are vacuum packaged in our
low-background storage bag to ensure purity.
DNPH Derivatization Process
Method Applications
US EPA IP-6A – Determination of Formaldehyde & Other Aldehydes
US EPA TO-11A – Method for Determination of Formaldehyde in
Ambient Air Using Adsorbent Cartridge Followed by HPLC
US EPA 100 – Sampling for Formaldehyde & Other Carbonyl
Compounds
ASTM Method D5197 – Standard Test Method for Determination of
Formaldehyde and Other Carbonyl Compounds
NIOSH 2016 – Formaldehyde
NIOSH 2532 – Glutaraldehyde
NIOSH 2539 – Aldehydes, Screening
NIOSH 2541 – Formaldehyde by GC
NIOSH 3500 – Formaldehyde by VIS; Impinger Method
OSHA 52 – Acrolein and/or Formaldehyde
OSHA 64 – Glutaraldehyde
OSHA 81 – Crotonaldehyde
OSHA 85 – Valeraldehyde
Air Monitoring Applications
Vapor Intrusion
Agricultural
Contaminants
Petrochemical
Industry
Paints & Coatings
Chinese Drywall
Contamination Welding Processes
Anesthetic Gases
in Healthcare
Scan code for detailed information on air sampling techniques and products for specific industries.
sigma-aldrich.com/airsampling
2
sigma-aldrich.com/air-monitoring
ORDER: 800-247-6628 (US only) 814-359-3441
Carbonyl Sample Collection Devices
Active Sampling
S10L Cartridge
The ORBO-555 is a 6 mm O.D. x 110 mm length dual-bed LpDNPH
glass sampling tube containing 300 mg of LpDNPH in the
front section and 150 mg in the back section. The back section
functions as a back-up bed for incidences of breakthrough
sometimes associated with single bed DNPH cartridges in higher
concentration environments or when you are not certain of the
concentration. The tube has a low pressure drop which enables
use at high sampling rates of 1.5 – 2.0 L/min, even with personal
sampling pumps.
Offers a reversible design for analysts who prefer shorter
dimensions and do not need an adaptor for sampling. The cartridge
is eluted by connecting to a syringe barrel that acts as a reservoir for
gravity-fed elution solvent. Meets EPA TO-11A requirements.
Cat. No.
505361-U
505358
Cat. No.
54020-U
Description
ORBO-555 DNPH Tube
Qty.
20
Description
LpDNPH S10L, 350 mg
LpDNPH S10L, 350 mg
Qty.
10
50
Air Sampling Products – Active Sampling Devices
NEW! ORBO™-555 Tube
S10x Cartridge
Shorter than the S10 cartridge and designed to fit automated
systems.
S10 Cartridge
Easy-to-use in the field and in the laboratory. Reusable adapters
are available for connecting the cartridge to the sampling pump.
Built-in reservoir eliminates the need to attach to a syringe
for sample extraction/elution. All S10 cartridges are 3 mL and
composed of a low extractable polypropylene syringe barrel
packed with 350 mg of LpDNPH. A starter kit with cartridges,
adapter and fittings needed is available.
Cat. No.
505293
Description
LpDNPH S10x, 350 mg
Qty.
10
Rezorian™ DNPH Cartridge
Cat. No.
Description
Vacuum Foil Packaging
21024-U
21026-U
21014
Qty.
LpDNPH S10 Starter Kit*
LpDNPH S10, 350 mg/3 mL
LpDNPH S10, 350 mg/3 mL
Made of low extractable polypropylene with polyethylene frits.
The end-fittings are luer lock syringe connections that can be
used individually or connected in a series (piggybacked) to
monitor breakthrough or to increase capacity.
10
10
50
Nylon Bag Packaging
23124-U
LpDNPH S10, Bulk
50
54072-U
LpDNPH S10 (1 tube/polyethylene bag)
50
* Includes one tube adapter and ten ¹/₈" male luer fittings.
Cat. No.
54074-U
54075-U
Description
Rezorian DNPH, 350 mg
Rezorian DNPH, 350 mg
Qty.
10
50
Above: LpDNPH S10 Starter Kit (21024-U)
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
3
Air Sampling Products – Active Sampling Devices
BPE-DNPH Cartridge
DNPH Coated Glass Fiber Filters
The BPE-DNPH cartridge is dual-layered and is comprised of silica
gels impregnated with trans-1,2-bis (2-pyridyl) ethylene (BPE) as
the top layer and the bottom layer of DNPH coated silica gel. The
BPE converts the ozone into a DNPH derivative for either analysis
or simply scrubbing ozone from your sample, depending on your
choice of extraction method. The DNPH layer collects carbonyls
for analysis. This product is not affected by high humidity.
Suitable for OSHA 64, 81 and 85 test methods for sampling
select carbonyls, such as glutaraldehyde, crotonaldehyde and
valeraldehyde.
Cat. No.
Description
Cartridges
54278-U
BPE-DNPH 130 mg/270 mg
54279-U
BPE-DNPH 130 mg/270 mg
Analytical Standard (for ozone analysis)
40117-U
Pyridine-2-Aldehyde DNPH (in acetonitrile,
aldehyde equivalent)
Qty.
10
50
3 x 2 mL
H Series Cartridges
The H series of LpDNPH cartridges contains higher loadings of
2,4-DNPH on the support material and larger bed weights
compared to the S10 cartridges. This provides a much higher
capacity for carbonyls making the H series cartridges the preferred
choice for use in high concentration environments. The H series is
available in H10 (350 mg), H30 (1 g) and H300 (10 g) cartridges.
Cat. No.
20069
Description
ORBO-827 LpDNPH Coated GFF, 37 mm
Ozone Scrubber
Available in Rezorian and reversible tube styles. Each tube style
contains 1.5 g of high purity potassium iodide. KI traps the ozone,
which causes a negative formaldehyde interference in DNPHcoated devices. Luer end-fittings enable you to connect this
cartridge directly to the inlet of any DNPH cartridge with a luer tip.
Testing (200 ppb ozone, 50% RH, 25 ºC) has shown the scrubber to
have an ozone capacity of 100,000 ppb/hr.
Cat. No.
54078-U
505285
Cat. No.
505315
505323
505331
505320-U
Description
LpDNPH H10, 350 mg/3 mL
LpDNPH H30, 1 g/6 mL
LpDNPH H300, 10 g/ 20 mL
LpDNPH H10, 350 mg/3 mL
Qty.
10
10
10
50
Qty.
25
Description
Rezorian, 1.5 g KI
Reversible, 1.5 g KI
Qty.
10
10
2-HMP on Amberlite® XAD®-2 for Formaldehyde
Commonly used for Industrial Hygiene (IH) sampling, OSHA 52
specifies use of a glass tube packed with 2-(Hydroxymethyl)
piperidine on Amberlite XAD-2. The 2-HMP on XAD-2 reacts with
formaldehyde to form an oxazolidine derivative. Suitable for NIOSH
2541 and OSHA 52 methods.
ORBO™-DNPH Tube
The ORBO-DNPH tube contains 120 mg of 2,4-DNPH packed into
a glass tube with a 'frangible' break seal ensuring purity until use.
Each tube measures 6 mm O.D. x 90 mm long.
Cat. No.
20081-U
4
Description
ORBO-DNPH, 120 mg
sigma-aldrich.com/air-monitoring
Qty.
10
Cat. No.
20257-U
20231
20357
Tube
ORBO-23
ORBO-24
ORBO-25
ORDER: 800-247-6628 (US only) 814-359-3441
Bed Wt. A/B
120 mg/60 mg
150 mg/75 mg
450 mg/225 mg
Dimensions
6 mm O.D. x 85 mm L
6 mm O.D. x 105 mm L
8 mm O.D. x115 mm L
Qty.
25
25
25
Solution Sampling
Supelco's Radiello and DSD-DNPH employ a radial passive
sampling design while other samplers commonly available in the
market employ an axial design. The benefits of the radial design
over the axial design are faster sampling rates, higher capacity and
better robustness to wind, temperature and humidity. They also
are versatile and can be used for indoor and outdoor air as well as
personal sampling.
Borosilicate Standard Glass Impingers and Bubblers
Radiello®
The Radiello passive sampler has sampling rates equivalent to
pumped (active) sampling. For example, the sampling rate for
formaldehyde is 99 mL/min and 84 mL/min for acetaldehyde.
Cat. No.
RAD165
RAD1201
RAD121
RAD122
Description
Aldehyde Cartridge Adsorbents
Blue Diffusive Body
Triangular Support Plate
Vertical Adapter (for personal sampling)
Qty.
20
20
20
20
For more information about Radiello Passive Sampling Products,
request the Radiello Brochure (IXV). To learn more about
Radiello Test Methods, visit us at sigma-aldrich.com/radiello to
download the Radiello Manual (IYP).
Glass impingers (for particles) and bubblers (for gases and vapors)
are ideal for NIOSH & OSHA methods that require collection of
airborne contaminants by drawing them into solution; available
with ground glass joints or threaded PTFE micro-connectors.
Length:
Reservoir Length:
Reservoir Capacity:
Graduations:
Glass Joint:
Impinger:
Bubbler:
186 mm (7.3 in)
152 mm (6 in)
25 mL
5 mL
24/40 taper
Standard Glass Stem
Fritted Glass Stem
Cat. No.
20270-U
Description
Std Midget Impinger
64835-U
Std Midget Bubbler
Air Sampling Products – Passive Sampling Devices
Passive Sampling
Qty.
1
1
Plastic Clips/PTFE Sleeves
Plastic clips fit over the connection on our 24/40 taper ground glass
joints to ensure secure connections. Use full-length PTFE sleeves
in ground glass joints for inert, tight seals without the possibility of
frozen joints; for use with standard impingers and bubblers.
DSD-DNPH
We offer another type of radial diffusive sampler known as
DSD-DNPH. It is comprised of a porous polyethylene tube, which
acts as the diffusive membrane, which is attached to a small
syringe barrel for elution of analysis from the adsorbent.
A 2,4-DNPH coated support acts as the adsorbent and moves
from the diffusive end during sample collection to the syringe
end for sample extraction by inverting the device. DSD-DNPH is
an all-in-one sample collection and elution device. Specified in
OSHA 1007 Method for Determination of Aldehydes.
Cat. No.
28221-U
Description
DSD-DNPH Sampling Device
Qty.
10
28222-U
000J004
Perforated Holder (for personal sampling)
DSD-DNPH Color Cap Insert (string not included)
10
100
Cat. No.
64764
Description
Plastic Clip for use with 24/40 taper
64761
PTFE Sleeve for use with 24/40 taper
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
Qty.
1
1
5
Air Sampling Products – Solution Sampling Devices
Borosilicate Threaded Midget Impingers and Bubblers
Make your sampling process more convenient. The vial can be
capped after sampling, thus reducing sample handling in the
field – no transferring of samples from the reservoir to a separate
vial. The reservoir may be easily replaced with a standard or
graduated screw-top vial.
Threaded Midget Impinger
Length (without vial):
Vial Capacity (mL):
Thread (mm):
Pack Size (ea):
Cat. No.:
143 mm (5 5/8 in)
22
20
2
64712-U
Threaded Midget Bubbler
Length (without vial):
Vial Capacity (mL):
Thread (mm):
Graduation Mark (mL):
Pack Size (ea):
Cat. No.:
143 mm (5 5/8 in)
22
20
15
1
64834-U
Spill Resistant Midget Bubbler
Length (without vial):
Vial Capacity (mL):
Thread (mm):
Graduation Mark (mL):
Pack Size (ea):
Cat. No.:
143 mm (5 5/8 in)
40
24
15
1
64832
Bottom cap allows easy emptying. Has a15 mL capacity for
absorbing solution. Can be packed with charcoal or other
adsorbent (sold separately). Cap and PTFE liner included.
Length 152 mm (6 in): 20 mm threads.
Insert your impinger, bubbler or in-line trap in this holder,
and attach to your lapel, shirt pocket or belt.
Cat. No.
64833
Description
In-line Impinger Trap w/20 mm Thread
20271
Impinger Holder
Qty.
1
1
NIOSH 3500 for Formaldehyde Impinger Method
(cap not included)
Cat. No.
Clear Vials
Description
Qty.
27173
27184
27379
22 mL 23 mm x 85 mm, thread 24-400
100
40 mL 29 mm x 82 mm, thread 24-400
This method has been replaced by solid sorbent sampling
methods, however it is still used in reference sampling situations.
This method utilizes impingers and filters, employing a solution
of chromotrophic acid. The formaldehyde is measured by
spectrophotometry.
Cat. No.
23383
Description
PTFE Filter w/Pads, 37 mm, 1.0 µm
Qty.
100
100
23369
37 mm Filter Cassette, 2 pc
100
40 mL 28 mm x 95 mm, thread 24-400
100
Reagents & Solutions
22 mL, 23 mm x 85 mm, thread 20-400
100
40 mL, 29 mm x 82 mm, thread 24-400
100
Cat. No.
252549-25ML
Description
Formaldehyde, ACS Reagent 37% in Water
40 mL, 28 mm x 95 mm, thread 24-400
100
27150-10G-F
Chromotropic Acid Disodium Salt,
Dihydrate
Sodium Bisulfite Solution (40%)
Sulfuric Acid, Concentrated,
ACS Reagent
Amber Vials
Caps for 22 mL Vials (Size: 20-400)
27174-U
Green Melamine Resin, Solid Cap, PTFE Liner
27175-U
Black Phenolic Solid Cap, Aluminum Liner
Caps for 40 mL Vials (Size: 24-400)
27186
Green Melamine Resin, Solid Cap, PTFE Liner
SU860006
White Polypropylene Solid Cap, PTFE/Silicone
6
In-Line Impinger Trap
Impinger Holder
Screw Top Replacement Vials
27073-U
27185-U
27382
Accessories for Solution Sampling
sigma-aldrich.com/air-monitoring
100
100
13438-1L-R
320501-500ML
100
100
ORDER: 800-247-6628 (US only) 814-359-3441
Qty.
25 mL
10 g
1L
500 mL
Air Sampling Accessories
Universal Elution Rack
Adapters, Fittings and Connectors
•1, 3 or 6 mL syringe style cartridges (S10)
•Closed cartridges (S10L)
•5 or 10 mL volumetric flasks
•2 or 4 mL vials
•Test tubes up to 15 mm ID x 10 cm
Cat. No.
21018-U
Description
Cartridge Adapters for S10, H10, H30
Qty.
10
57267
21016
23364
24856
21017
21015
25064-U
504351
57098
21019-U
21012
57242
20015-U
Cartridge Adapters for H300
Male Luer Fittings for 1/8” Tubing
Male Luer Fittings for 3/16” Tubing
Male Luer Fittings for 1/4” Tubing
Fittings for 1/8” Tubing
Female Luer Couplers
Male Luer Couplers
Male Luer Plugs
Visidry™ Female Luer Plug
Lapel Clips
Bar Code Labels
Syringe Barrels, 6 mL
Glass Reservoirs, 5 mL
6
20
20
10
20
20
20
12
12
6
100
30
5
Cartridge Adapter
21018-U
With cartridge adapters (for S10 or H300), you can attach an
empty syringe barrel to the cartridge to serve as a solvent
reservoir. The rack allows room for syringe filters.
Cat. No.
21043-U
Description
Universal Elution Rack
Air Sampling Products – Accessories
We offer a selection of reusable adapters and fittings for
connecting our cartridges to a sampling pump and other devices.
Developed for fast and convenient sample preparation without
the use of a vacuum. Our versatile elution rack can be used with
a variety of air monitoring tubes and receiving vessels, including
our LpDNPH cartridges, for simultaneous gravity feed extraction
up to 12 samples. By using the assembly plates in various
combinations, you can configure the unit to accept:
Qty.
1
Cartridge Adapter
57267
Male Luer Fitting (for 1/8” tubing)
21016
Male Luer Fitting (for 3/16” tubing)
23364
Male Luer Fitting (for 1/4” tubing)
24856
Female Luer Fitting
21017
Female Luer Coupler
21015
Male Luer Coupler
25064-U
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
7
Sampling Pumps
PAS-500 Micro Air Sampler
Air Sampling Products – Sampling Pumps
Escort Elf® Sampling Pump
An electronic laminar flow sensor in this easy-to-operate,
state-of-the-art sampling pump provides constant flow control,
unaffected by changes in battery voltage, temperature, sample
load or altitude. An internal secondary standard calibrates the
pump continuously, requiring only monthly calibration with a
primary standard. A built-in counter monitors total operating
time and reminds you when a primary calibration is required. The
pump also features a low battery function with an indicator light
and blocked flow detection. LED readout alternately displays flow
rate and elapsed sampling time. Order charger separately.
This low flow pump is lightweight (4 oz./114 g) and compact
(7 in./17.8 cm), fitting easily into your shirt pocket. The adsorbent
tube connects directly to the inlet of the pump. This sampler is
versatile, adapts to fit both 6 and 8 mm O.D. tubes, and the flow
range is 40-200 mL/min. The low flow adapter enables you to
sample at 20 mL/min.
This unit is powered by a convenient and easily replaceable 9-volt
battery. The full flow regulation feature provides constant voltage
to the pump, even as battery voltage drops. It is intrinsically safe – a
built in resistor limits the power current, preventing any short circuit.
Twin Port Sampler
This pump attachment is designed for low flow industrial hygiene
sampling, such as gas and vapor monitoring, using sorbent
tubes. Two needle valves provide independent flow control for
simultaneous collection on two tubes, but can also be used
for a single tube by closing the flow through one valve. The
sampler is compatible with any personal sampling pump capable
of 1.5 L/min flow rate and a load of 25 in of water. Total flow
cannot exceed 500 mL/min. Each sampler comes with two tube
protectors, one for small tubes (2 in./ 5 cm long) and one for large
tubes, (<4.5 in. / 12.5 cm long) and the tubing required to connect
the sampler to the sampling pump.
Cat. No. Description
PAS-500 Micro Air Sampler with Low Flow Orifice
24865
Includes sampler, 6 mm O.D. tube holder,
screwdriver and two 9-Volt batteries
Tube Holder for PAS-500 Pump
24867
For use with 6 mm O.D. adsorbent tube
24868
For use with detector tube
24869
For use with 8 mm O.D. adsorbent tube
Carrying Case for PAS-500
24871
Single pump case
Qty.
1
1
1
1
1
Flow Calibration Devices for Air Sampling Pumps
Cat. No.
28160-U
Description
Escort Elf Sampling Pump
Qty.
1
28118-U
Twin Port Sampler
1
Accessories
28155-U
28157-U
28158-U
28159-U
Cat. No. Description
Mini-Buck Flow Calibrator
Omega Battery Charger 12 Volt
110 Volt, units charged: 1
240 Volt, units charged: 1
120 Volt/240 Volt, units charged: 5
1
1
1
1
24843
Model M-5, Flow Rate 1-6000 mL/min
1
24845
24844
24846
Model M-30, Flow Rate 1-30 mL/min
Battery Charger for M-5/M-30, 110 Volt*
Battery Charger for M-5/M-30, 220 Volt*
1
1
1
Model 1067 Ambient Air Sampler
Cat. No.
507113
Description
Model 1067 Tube Sampler* (Dual Channel)
24697-U
Universal Charger, 110 V/240 V
*Battery charger not included with 24843 and 24845, order separately
Optiflow Digital Bubble Flow Meter
Qty.
1
1
Cat. No.
28679-U
*Includes universal charger
8
sigma-aldrich.com/air-monitoring
Qty.
ORDER: 800-247-6628 (US only) 814-359-3441
Description
Model 520, Flow Range: 0.5-500 mL/min
Qty.
1
Analytical Columns for Analysis of Carbonyl Samples
Analytical Columns for GC Analysis
SUPELCOWAX® 10
Application: This column is based on one of the most widely
used polar phases and is suitable for analyses
of carbonyls, solvents, fatty acid methyl esters
(FAMEs), food, flavor and fragrance compounds,
alcohols and aromatics.
Analytical Columns for HPLC Analysis
Additionally, this column is a great choice when a
polar general purpose column is required.
Ascentis® Express C18 and RP-Amide
Ascentis Express HPLC columns, through the use of Fused-Core®
particle technology, can provide you with both the high speed
and high efficiencies of sub-2 μm particles while maintaining
lower backpressures. The combination of high efficiency and low
backpressure in conjunction with robustness benefits UPLC®
(or other ultra high pressure system) users as well as conventional
HPLC users.
Suitable for OSHA 52
USP Code: This column meets USP G16 requirements.
Phase: Bonded; poly(ethylene glycol)
Temp. Limits: ≤0.53 mm I.D., df <2 μm: 35 °C to 280 °C
(isothermal or programmed)
Air Sampling Products – HPLC and GC Columns
After sample collection of carbonyls by the wide range of
sample collection devices, the analytes are typically analyzed by
HPLC with a UV detector; an exception to this is the OSHA 52
method whose modified method employs capillary GC analysis.
In addition to carbonyl sample collection devices, we provide
the complete solution of analytical standards, neats, reagents,
solutions and analytical HPLC and GC columns.
Ascentis Express Particles
0.6 µm
0.5 µm
~5 µm
2.7 µm
1.7 µm
3.3 µm
Solid Core
2.7 µm
Cat. No.
Description
Ascentis Express C18 HPLC Column
53829-U
Solid Core
Cat. No.
Description
SUPELCOWAX 10 Capillary Column
5 µm
Qty.
15 cm x 4.6 mm I.D, 2.7 µm
1
Ascentis Express RP-Amide HPLC Column
53931-U
15 cm x 4.6 mm I.D, 2.7 µm
1
25325
30 m × 0.53 mm I.D., 0.5 μm
Qty.
1
For more information, visit
sigma-aldrich.com/gc
For more information, visit
sigma-aldrich.com/express
D
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
9
BPE-DNPH – Acetonitrile (3 mL) Extraction
column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 3 µm particles (565322-U)
mobile phase A: 60:40, water:acetonitrile
mobile phase B: 25:75, water:acetonitrile
flow rate: 1.5 mL/min.
1. Dinitrophenylhydrazine (100 µg/mL)
temp.: 30 °C
2. Formaldehyde-2,4-DNPH (40 µg/mL)
det.: UV at 360 nm
3. Acetaldehyde-2,4-DNPH (29 µg/mL)
injection: 10 µL
4. Acetone-2,4-DNPH (23 µg/mL)
sample: as listed in mobile phase
5. Acrolein-2,4-DNPH (24 µg/mL)
gradient: Min %A
%B
6. Propionaldehyde-2,4-DNPH (23 µg/mL)
0 100
0
7. Crotonaldehyde-2,4-DNPH (20 µg/mL)
5 100
0
8. 2-Butanone-2,4-DNPH (10 µg/mL)
25
40
60
9. Butyraldehyde-2,4-DNPH (20 µg/mL)
40
0 100
10. Benzaldehyde-2,4-DNPH (15 µg/mL)
60
0 100
11.
12.
13.
14.
15.
16.
17.
18.
1
2
3
5
4 6
Cyclohexanone-2,4-DNPH (10 µg/mL)
Isovaleraldehyde-2,4-DNPH (18 µg/mL)
Valeraldehyde-2,4-DNPH (18 µg/mL)
o-Tolualdehyde-2,4-DNPH (14 µg/mL)
m-Tolualdehyde-2,4-DNPH (14 µg/mL)
p-Tolualdehyde-2,4-DNPH (14 µg/mL)
Hexaldehyde-2,4-DNPH (16 µg/mL)
2,5-Dimethylbenzaldehyde-2,4-DNPH
(13 µg/mL)
Heptaldehyde-2,4-DNPH (19 µg/mL)
Octaldehyde-2,4-DNPH (19 µg/mL)
Nonaldehyde-2,4-DNPH (19 µg/mL)
Decylaldehyde-2,4-DNPH (19 µg/mL)
19.
20.
21.
22.
10,11 13
12 14 15,16
9
17 19
8
18
7
column:
mobile phase:
flow rate:
det.:
injection:
Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particle
55:45 acetonitrile:water (4 min), gradient to
90% acetonitrile at 13 min (5 min hold)
0.5 mL/min
UV 360 nm
10 μL
Air Sample
DNPH
3
1
400
This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of
21 aldehyde ketone derivatives.
1.
2.
3.
4.
5.
4
Formaldehyde-DNPH
Pyridine-2-aldehyde-DNPH*
Acetaldehyde-DNPH
Acetone-DNPH
Hexanal-DNPH
*Peak 2 is a marker for ozone presence.
2
mAU
200
Analysis of 21 Aldehyde/Ketone DNPH Derivatives Using
Ascentis RP-Amide
5
BPE
0
Carbonyl Sampling – Applications
Analysis of Carbonyl Samples
0
2
4
6
8
10
Min
12
14
16
18
12
14
16
18
Cartridge Blank
21
20
22
BPE
DNPH
mAU
10
0 102030 405060
Min
G003341
column: Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 µm (53827-U)
mobile phase: 60:40, water:acetonitrile
flow rate: 2 mL/min.
temp.: 30 °C
det.: UV at 360 nm
injection: 5 µL
1. Formaldehyde-2,4-DNPH
7. 2-Butanone-2,4-DNPH
sample:water
2.
3.
4.
5.
6.
1
0
Acetaldehyde-2,4-DNPH
8. Butyraldehyde-2,4-DNPH
Acetone-2,4-DNPH
9. Benzaldehyde-2,4-DNPH
Acrolein-2,4-DNPH
10. Valeraldehyde-2,4-DNPH
Propionaldehyde-2,4-DNPH 11. m-Tolualdehyde-2,4-DNPH
Crotonaldehyde-2,4-DNPH 12. Hexaldehyde-2,4-DNPH
13. Methacrolein-2,4-DNPH
R
HN
O
NH
1
N
+
O
2
1.0
3
4
5
6 78
9
2.0
O
-
N
O
11
3.0
Min
10
-
+
10
sigma-aldrich.com/air-monitoring
13
12
4.0
5.0
2
6.0
G005382
ORDER: 800-247-6628 (US only) 814-359-3441
2
4
6
4
3
0
Isocratic Analysis of Aldehydes/Ketone DNPH on
Ascentis Express C18
8
10
Min
G005758-G005759
Acetonitrile Extract of ORBO-555 Tube (Primary Bed)
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm (53829-U)
mobile phase A: water
mobile phase B: acetonitrile
flow rate: 0.5 mL/min
temp.:ambient
det.: UV at 360 nm
injection: 10 µL
gradient: Time (min)
%A
%B
0
45
55
4
45
55
13
10
90
20
10
90
column: Ascentis Express C18, 15cm x 4.6mm I.D.,
2.7 μm particles (53829-U)
mobile phase A: water
mobile phase B: acetonitrile
flow rate: 0.5 mL/min.
temp.:ambient
det.: UV, 360 nm
injection: 10 µL
sample cartridge: ORBO-555 DNPH Cartridge (54020-U)
gradient:Min %A %B
0
45
55
4
45
55
13
10
90
20
10
90
1.
2.
3.
4.
5.
LpDNPH S10 Cartridge Blank
DNPH
mAU
60
Carbonyl Sampling – Applications
Fast Separation on Fused-Core Ascentis Express Column
2,4-Dinitrophenylhydrazine (DNPH)
Formaldehyde-DNPH
Acetaldehyde-DNPH
Acetone-DNPH
Hexanal-DNPH
1
2
40
40
20
4
0 1020
Min
G005426
mAU
0
20
DNPH
3
mAU
60
5
3
LpDNPH S10 Cartridge Blank Spiked with DNPH Standards
1. Formaldehyde-DNPH
2. Acetaldehyde-DNPH
3. Acetone-DNPH
0
0 2 4 6 8 1012141618
Min
G005548
40
1
20
2
Blank Tube (Primary Bed)
0
1
0 1020
Min
G005427
8
6
LpDNPH S10 Cartridge – Laboratory Air Sample
mAU
60
mAU
3
DNPH
4
40
2
1
20
2
2
0
0
0 1020
Min
G005428
3
4
0 2 4 6 8 1012141618
Min
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
G005549
11
Carbonyl Sampling – Standards
Air Monitoring Standards for Analysis of
Carbonyl Samples
American Society for Testing and Materials
(ASTM) Methods
California Air Resources Board (CARB) Methods Analysis of Carbonyls in Ambient Air
The following standards are for use with methods developed
under ASTM Committee D-22, described in the Annual Book of
ASTM Methods, Volume 11.03, Atmospheric Analysis, Occupational
Health and Safety. The standards are quantitative formulations for
use as chromatographic calibration or spiking solutions. Products
include a Certificate of Analysis describing lot-specific production
and analytical information. Free data packets are available for most
of these products. Data packets contain data on raw materials
and final production. Request the data packet when ordering the
standard; the order number is the same as that for the standard,
preceded by the letters DP.
California Air Resources Board (CARB) – The following quantitative
formulations were developed to support the analysis of
aldehydes in ambient air by CARB Method 1004. Analysis is of
the dinitrophenylhydrazine (DNPH) derivatives by HPLC-UV.
Concentrations stated are of the equivalent carbonyl before
derivatization, except where noted. The Certificate of Analysis
accompanying these products states both DNPH derivatized and
non-derivatized concentrations.
ASTM D5197 Method: Determination of Formaldehyde
and Other Carbonyl Compounds in Air
Acetaldehyde-2,4-dinitrophenylhydrazone, 1000 μg/mL
Acetone-2,4-dinitrophenylhydrazone, 500 μg/mL
Acrolein-2,4-dinitrophenylhydrazone, 500 μg/mL
Benzaldehyde-2,4-dinitrophenylhydrazone, 500 μg/mL
Butyraldehyde-2,4-dinitrophenylhydrazone, 500 μg/mL
Formaldehyde-2,4-dinitrophenylhydrazone, 1500 μg/mL
Cat. No.
47285-U
Description
Concentration
TO11/IP-6A Aldehyde/ 15 µg/mL in acetonitrile,
Ketone-DNPH Mix
as aldehyde and ketone
equivalent
Acetaldehyde-2,4-dinitrophenylhydrazone
Acetone-2,4-dinitrophenylhydrazone
Acrolein-2,4-dinitrophenylhydrazone
Benzaldehyde-2,4-dinitrophenylhydrazone
Butyraldehyde-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
2,5-Dimethylbenzaldehyde-2,4-dinitrophenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone
Hexaldehyde-2,4-dinitrophenylhydrazone
Isovaleraldehyde-2,4-dinitrophenylhydrazone
Propionaldehyde-2,4-dinitrophenylhydrazone
o-Tolualdehyde-2,4-dinitrophenylhydrazone
m-Tolualdehyde-2,4-dinitrophenylhydrazone
p-Tolualdehyde-2,4-dinitrophenylhydrazone
Valeraldehyde-2,4-dinitrophenylhydrazone
12
sigma-aldrich.com/air-monitoring
Qty.
1 mL
Cat. No.
47649-U
Description
Concentration
CARB Carbonyl-DNPH in acetonitrile, varied,
Mix 1
derivative concentration
Cat. No.
47650-U
Description
CARB Method 1004
DNPH Mix 1
47651-U
CARB Method 1004
DNPH Mix 2
Concentration
3 µg/mL in acetonitrile,
as aldehyde and ketone
equivalent
3 µg/mL in acetonitrile,
as aldehyde and ketone
equivalent
Acetaldehyde-2,4-dinitrophenylhydrazone
Acetone-2,4-dinitrophenylhydrazone
Acrolein-2,4-dinitrophenylhydrazone
Benzaldehyde-2,4-dinitrophenylhydrazone
2-Butanone-2,4-dinitrophenylhydrazone
Butyraldehyde-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone
Hexaldehyde-2,4-dinitrophenylhydrazone
Methacrolein-2,4-dinitrophenylhydrazone
Propionaldehyde-2,4-dinitrophenylhydrazone
m-Tolualdehyde-2,4-dinitrophenylhydrazone
Valeraldehyde-2,4-dinitrophenylhydrazone
ORDER: 800-247-6628 (US only) 814-359-3441
Qty.
1 mL
Qty.
1 mL
1 mL
US EPA TO Methods
DNPH Mixes
Toxic Organic Compounds in Air (TO)
The following dinitrophenylhydrazine (DNPH) standards were
developed in response to European requests for working and
calibration check standards for the ambient air analysis of carbonyl
emissions from automobile exhaust. Methods for this analysis are
equivalent to California Air Resources Board 1004 (Sacramento,
CA, USA). Concentrations are of the equivalent carbonyl quantity
before derivatization. The Certificate of Analysis accompanying
each of these products states both DNPH-derivatized and
non-derivatized concentrations.
TO-5/TO-11: Aldehydes and Ketones by HPLC/UV
Cat. No. Description
Concentration
Qty.
47672-U Carbonyl-DNPH Mix 1 20 µg/mL in acetonitrile, except 1 mL
where indicated (as aldehyde
and ketone equivalent)
Acetaldehyde-2,4-dinitrophenylhydrazone
Acetone-2,4-dinitrophenylhydrazone
Acrolein-2,4-dinitrophenylhydrazone
Benzaldehyde-2,4-dinitrophenylhydrazone
2-Butanone-2,4-dinitrophenylhydrazone
Butyraldehyde-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone, 40 μg/mL
Hexaldehyde-2,4-dinitrophenylhydrazone
Methacrolein-2,4-dinitrophenylhydrazone
Propionaldehyde-2,4-dinitrophenylhydrazone
p-Tolualdehyde-2,4-dinitrophenylhydrazone
Valeraldehyde-2,4-dinitrophenylhydrazone
Cat. No.
47671-U
Description
TO-11A Six
Component
Carbonyl-DNPH Mix
Concentration
15 µg/mL in acetonitrile
(as aldehyde and ketone
equivalent)
Qty.
1.5 mL
Acetaldehyde-2,4-dinitrophenylhydrazone
Acrolein-2,4-dinitrophenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone
Acetone-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
Propionaldehyde-2,4-dinitrophenylhydrazone
Radiello Aldehyde Calibration Standard
The aldehyde calibration standard consists of nine
2,4-dinitrophenylhydrazones (2,4-DNPH) diluted in acetonitrile.
Actual concentrations for each component are certified for each
lot. The standard stock solution is shipped in a pierceable-septum
crimped cap. Cartridges are stable for at least four months when
stored at 4 °C.
Cat. No. Description
RAD302 Aldehyde
Calibration Std
DNPH Single-Component Solution
Concentration
500 μg/mL in acetonitrile
1 mL
3 x 1 mL
Acetaldehyde-2,4-dinitrophenylhydrazone
Acetone-2,4-dinitrophenylhydrazone
Acrolein-2,4-dinitrophenylhydrazone
Benzaldehyde-2,4-dinitrophenylhydrazone
Butyraldehyde-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
2,5-Dimethylbenzaldehyde-2,4-dinitro-phenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone
Cat. No.
48149-U
Description
Concentration
Qty.
Carbonyl-DNPH Mix 2 2 µg/mL in acetonitrile, except 1 mL
where indicated (as aldehyde
and ketone equivalent)
Description
Cyclohexanone-2,4DNPH solution
Qty.
TO-11A Formaldehyde by HPLC
Acetaldehyde-2,4-dinitrophenylhydrazone
Cyclohexanone-2,4-dinitrophenylhydrazone, 5 μg/mL
Acetone-2,4-dinitrophenylhydrazone
Formaldehyde-2,4-dinitrophenylhydrazone, 4 μg/mL
Acrolein-2,4-dinitrophenylhydrazone
Hexaldehyde-2,4-dinitrophenylhydrazone
Benzaldehyde-2,4-dinitrophenylhydrazone
Methacrolein-2,4-dinitrophenylhydrazone
2-Butanone-2,4-dinitrophenylhydrazone
Propionaldehyde-2,4-dinitrophenylhydrazone
Butyraldehyde-2,4-dinitrophenylhydrazone
p-Tolualdehyde-2,4-dinitrophenylhydrazone
Crotonaldehyde-2,4-dinitrophenylhydrazone
Valeraldehyde-2,4-dinitrophenylhydrazone
Cat. No.
47673-U
Cat. No.
Description
Concentration
Standard type calibration
47285-U TO11/IP-6A Aldehyde/ 15 µg/mL in acetonitrile
4M7285-U Ketone-DNPH Mix
(as aldehyde and ketone
equivalent)
Carbonyl Sampling – Standards
European Mixes
Qty.
1 mL
Concentration
Qty.
50 µg/mL in acetonitrile, except 10 mL
where indicated (as aldehyde
and ketone equivalent)
Acetaldehyde-2,4-DNPH
Acrolein-2,4-DNPH, 10 µg/mL
Benzaldehyde-2,4-DNPH
Butanal-2,4-DNPH
Formaldehyde-2,4-DNPH
Hexanal-2,4-DNPH
Isopentanal-2,4-DNPH
Pentanal-2,4-DNPH
Propanal-2,4-DNPH
TECHNICAL SERVICE: 800-359-3041 (US and Canada Only) 814-359-3041
13
Carbonyl Sampling – Standards
Aldehyde and Ketone DNPH Derivatives,
Neats & Solutions
These solutions of DNPH derivatives are designed as quantitative
calibration mixtures where a multi-component solution is not
suitable. At concentration indicated as aldehyde or ketone
equivalent in 1 mL (actual filling 1.1 - 1.2 mL) acetonitrile in amber
glass ampul.
Cat. No.
442321-U
Description
2,5-Dimethylbenzaldehyde2,4-DNPH
442339
2-Butanone-2,4-DNPH
47344
2-Butanone-2,4-DNPH
solution
47340-U
Acetaldehyde-2,4-DNPH
solution
4M7340-U Acetaldehyde-2,4-DNPH
solution
442434
Acetaldehyde-2,4-DNPH
442436
Acetone-2,4-DNPH
47342
Acrolein-2,4-DNPH solution
442441
47343
442469
47345-U
442504
47175-U
442529
47673-U
442533
33852
47177
4M7177
14
Acrolein-2,4-DNPH
Benzaldehyde-2,4-DNPH
solution
Benzaldehyde-2,4-DNPH
Butyraldehyde-2,4-DNPH
solution
Butyraldehyde-2,4-DNPH
Crotonaldehyde-2,4-DNPH
solution
Crotonaldehyde-2,4-DNPH
Cyclohexanone DNPH
solution
Cyclohexanone DNPH
Decanal
2,4-dinitrophenylhydrazone
Formaldehyde-2,4-DNPH
solution
Formaldehyde-2,4-DNPH
solution
sigma-aldrich.com/air-monitoring
Concentration
100 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
500 μg/mL in
acetonitrile
Qty.
100 mg
100 mg
1 mL
47564-U
33848
47178-U
442614
47886
47179
1 mL
47180-U
5 x 1 mL
100 mg
50 mg
1 mL
25 mg
1 mL
100 mg
1 mL
100 mg
1 mL
100 mg
1 mL
10 mg
100 mg
100 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
Cat. No.
442597
49208-U
1 mL
442639
33851
33849
47032-U
47183
47182
442722
47184-U
442735
47181
442768
47185-U
442834
5 x 1 mL
ORDER: 800-247-6628 (US only) 814-359-3441
Description
Formaldehyde-2,4-DNPH
Formaldehyde-2,4DNPHydrazide solution
Glutaraldehyde-2,4-DNPH
solution
Heptanal
2,4-dinitrophenylhydrazone
Hexaldehyde-2,4-DNPH
solution
Hexaldehyde-2,4-DNPH
Isobutyraldehyde-2,4-DNPH
solution
Isovaleraldehyde-2,4-DNPH
solution
Methacrolein-2,4-DNPH
solution
Methacrolein-2,4-DNPH
Nonanal
2,4-dinitrophenylhydrazone
Octanal
2,4-dinitrophenylhydrazone
o-Phthaldialdehyde-(DNPH)2
solution
m-Tolualdehyde-2,4-DNPH
solution
o-Tolualdehyde-2,4-DNPH
solution
o-Tolualdehyde-2,4-DNPH
p-Tolualdehyde-2,4-DNPH
solution
p-Tolualdehyde-2,4-DNPH
Propionaldehyde-2,4-DNPH
solution
Propionaldehyde-2,4-DNPH
Valeraldehyde-2,4-DNPH
solution
Valeraldehyde-2,4-DNPH
Concentration
100 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
Qty.
100 mg
3 x 2 mL
1 mL
100 mg
1000 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
1 mL
100 mg
1 mL
1 mL
1 mL
100 mg
100 mg
100 mg
10 μg/mL in
acetonitrile/
DMSO (7:3)
100 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
1000 μg/mL in
acetonitrile
100 μg/mL in
acetonitrile
1 mL
1 mL
1 mL
100 mg
1 mL
100 mg
1 mL
100 mg
1 mL
100 mg
Alternative Aldehyde and Ketone Derivatives
DAIH
NIOSH and OSHA Methods for Workplace Atmospheres
(2-Diphenylacetyl-indan-1,3-dione-1-ethylidenehydrazone)
derivatives of aldehydes and ketones. Material purity ≥ 98% by GC.
Free data packets containing data on raw materials and final
production are available for most products. Request the data
packet when ordering the standard; the order number is the same
as that for the standard, preceded by the letters DP.
Cat. No.
14423
02819
13173
Description
Acetaldehyde, DAIH derivative
Acetone, DAIH derivative
Acrolein, DAIH derivative
Qty.
50 mg
50 mg
50 mg
55556
91547
Crotonaldehyde, DAIH derivative
Cyclohexanone, DAIH derivative
50 mg
50 mg
06947
51299
Formaldehyde, DAIH derivative
Propionaldehyde, DAIH derivative
50 mg
50 mg
NIOSH 2541/OSHA 52: Analysis of Formaldehyde in Indoor Air
Cat. No.
48414
Description
Formaldehyde
Oxazolidine solution
Concentration
2000 μg/mL in toluene
Qty.
1 mL
Carbonyl Sampling – Standards
The following standards are for use with methods listed in
OSHA and NIOSH manuals of methods for analysis of workplace
contaminants. The standards are quantitative formulations for
use as chromatographic calibration or spiking solutions. Products
include a Certificate of Analysis describing lot-specific production
and analytical information.
Oximes
PFBHA (O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine) derivatives
do not decompose at an elevated temperature. For this reason,
PFBHA derivatives are a good alternative to 2,4-DNPH derivatives
when using GC. Material purity ≥ 98 % by GC except where noted.
Cat. No. Description
15875 Acetaldehyde-O-pentafluorphenylmethyl-oxime
purum
44114 Acetone-O-pentafluorphenylmethyl-oxime purum
65819 Acrolein-O-pentafluorphenylmethyl-oxime purum
(≥95%)
41558 Formaldehyde-O-pentafluorphenylmethyl-oxime
purum
03718 Glutaraldehyde bis-O-pentafluorophenylmethyloxime purum
43508 Propionaldehyde O-pentafluorophenylmethyl-oxime
purum
66156 Valeraldehyde O-pentafluorophenylmethyl-oxime
purum
Qty.
10 mg
10 mg
10 mg
10 mg
10 mg
10 mg
10 mg
50 mg
Get Started
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on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.
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