32-Channel Miniature Preamplifier Manual
32-Channel Miniature Preamplifier Manual Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it. In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document. © 2014 Multi Channel Systems MCS GmbH. All rights reserved. Printed: 11. 02. 2014 Multi Channel Systems MCS GmbH Aspenhaustraße 21 72770 Reutlingen Germany Fon +49-71 21-90 92 5 - 0 Fax +49-71 21-90 92 5 -11 [email protected] www.multichannelsystems.com Microsoft and Windows are registered trademarks of Microsoft Corporation. Products that are referred to in this document may be either trademarks and/or registered trademarks of their respective holders and should be noted as such. The publisher and the author make no claim to these trademark. Table of Contents 1 Important Information and Instruction 1 1.1 Operator's Obligations 1 1.2 Guarantee and Liability 1 1.3 Important Safety Advice 2 2 Welcome to the MPA32I 3 3 Setting Up and Connecting the MPA 4 3.1 General Setup Recommendations 4 3.2 Testing the Noise Level of the Setup 5 4 Setting up MC_Rack 6 5 Connecting FlexMEAs 8 5.1 EcoFlexMEAs 10 5.1.1 Connecting 32-Channel NeuroNexus Probes 11 5.1.2 Adapter-NN-32 Pin Layout 12 5.2 Connecting 54-Channel NeuroNexus Probes 13 6 Service and Maintenance 15 7 Pin Layout 16 7.1 Power Supply 16 7.2 Test Model Probe 17 8 Contact Information 18 9 Technical Specifications MPA32I 19 10 Datasheet 20 Important Information and Instruction 1 Important Information and Instruction 1.1 Operator's Obligations The operator is obliged to allow only persons to work on the device, who are familiar with the safety at work and accident prevention regulations and have been instructed how to use the device; are professionally qualified or have specialist knowledge and training and have received instruction in the use of the device; have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature. It must be monitored at regular intervals that the operating personnel are working safely. Personnel still undergoing training may only work on the device under the supervision of an experienced person. 1.2 Guarantee and Liability The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply. The operator will receive these no later than on conclusion of the contract. Multi Channel Systems MCS GmbH makes no Guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software. It is up to the user to use good laboratory practice to establish the validity of his findings. Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of the following. Improper use of the device. Improper installation, commissioning, operation or maintenance of the device. Operating the device when the safety and protective devices are defective and/or inoperable. Non-observance of the instructions in the manual with regard to transport, storage, installation, commissioning, operation or maintenance of the device. Unauthorized structural alterations to the device. Unauthorized modifications to the system settings. Inadequate monitoring of device components subject to wear. Improperly executed and unauthorized repairs. Unauthorized opening of the device or its components. Catastrophic events due to the effect of foreign bodies or acts of God. 1 32-Channel Miniature Preamplifier Manual 1.3 Important Safety Advice Warning: Make sure to read the following advices prior to install or to use the device and the software. If you do not fulfill all requirements stated below, this may lead to malfunctions or breakage of connected hardware, or even fatal injuries. Warning: Obey always the rules of local regulations and laws. Only qualified personnel should be allowed to perform laboratory work. Work according to good laboratory practice to obtain best results and to minimize risks. The product has been built to the state of the art and in accordance with recognized safety engineering rules. The device may only be used for its intended purpose; be used when in a perfect condition. Improper use could lead to serious, even fatal injuries to the user or third parties and damage to the device itself or other material damage. Warning: The device and the software are not intended for medical uses and must not be used on humans. Malfunctions which could impair safety should be rectified immediately. Any physical damage of the cable, such as a broken cable, causes a physical damage of the miniature preamplifier that cannot be repaired. Protect the device from heat. Do not autoclave! You can clean the connectors with distilled water in an ultra sonic bath, but keep the cable away from the fluid. Dry the device with compressed air. Use and keep the device always in a dry environment. Do not expose it to fluids or vapor for a longer period of time. 2 FlexMEAs 2 Welcome to the MPA32I The 32-Channel Miniature Preamplifier MPA32I is connected to the microelectrodes for providing the initial tenfold amplification stage. It has additional common ground and reference electrode inputs. The reference electrode is ideally identical to the recording electrodes and placed into a comparable but inactive area or tissue. Background or noise signals that are picked up by both the reference electrode and the recording electrodes are removed. The metal case provides electrical shielding. Electrode damage is prevented by the very low bias current. The high input impedance ensures stable long-term recordings: Ideally, the input impedance would be infinite. As low voltages are generally recorded, a high current would flow if the input impedance were low. As a result, the amplifier would not be able to deliver the current, and the voltage would break down. The miniature preamplifier has a high input impedance to avoid this problem. Adapters for all standard microelectrodes such as NeuroNexus probes (from www.neuronexustech.com) for acute and chronic implantations ensure a close proximity to the probe to minimize signal loss and cross talk. Adapters are not included in the standard scope of delivery and have to be ordered separately. For custom adapters, please ask your local retailer or contact Multi Channel Systems. 3 32-Channel Miniature Preamplifier Manual 3 Setting Up and Connecting the MPA 3.1 General Setup Recommendations In the following, you find general recommendations for the installation. Important: It is important that the complete setup refers to a single common ground. The reference input has always to be connected. It is recommended to use a reference electrode. However, if you are not using a reference electrode, connect the reference input to ground (GND). Otherwise, noise picked up by the reference input will be subtracted from the recording signals. This will either lead to signal loss or to a very high noise level. 1. Ground the animal with a ground electrode of large surface area, for example, a liquid gel adhesive electrode, that is connected to the ground of the setup, for example, a large metal table or a Faraday cage, to avoid pickup of noise from the environment. The ground electrode is best positioned in an electrically inactive region (not near muscle, nor heart), for example, at the belly. 2. Connect the GND input or the metal case of the miniature preamplifier to the common ground of the setup. (The GND input is internally connected to the metal case.) 3. Connect the reference electrode to the reference input of the miniature preamplifier. Generally, a reference electrode is inserted into non-active tissue of the experimental model. The reference electrode should be identical to the recording electrode so that both electrodes see the same background noise. This is necessary because despite the grounding, the animal’s body often has not exactly a potential of zero, due to the electrode impedance, for example. The background noise is then subtracted from the recording signal, increasing the signal to noise ratio. Please note that this may not work if the complete setup is not properly grounded. 4. (Optional) If two reference electrodes are used, connect the second electrode to the free reference input. Otherwise, leave it free. It is generally not necessary to use a second reference electrode. It can be used to enlarge the surface area of the reference, though. 5. Connect the recording electrodes to the recording channels of the miniature preamplifier. You can either use single electrodes or multitrodes with the miniature preamplifier. MCS provides adapters for standard probes. 4 FlexMEAs 6. Connect all unused recording channels to the GND input or to the reference electrode if the reference and ground electrode of the amplifier are short circuited, to avoid noise pickup. As the total amplifier gain generally lies in the range of 1000, even very small noise signals may generate high noise signals. 7. If you are not using a Faraday cage, it might be necessary to shield the complete setup with aluminum foil or similar to prevent electrical interference from the outside. Connect the aluminum foil to the ground of the setup (for example, the metal table). Troubleshooting: If you observe problems with noise, check that the ground of the setup is connected to exactly the same ground as the data acquisition computer. For example, connect the metal table to the ground / earth wire of a free power outlet (of the same electrical system), as the computer is generally connected to ground/earth via the power plug, too. Also, connect the data acquisition computer’s metal case with a thick ground wire to the ground of the setup (for example, the metal table), preferably with a 4 mm plug. 3.2 Testing the Noise Level of the Setup A terminal plug that connects all inputs to ground, and a test model probe that mimics the electrode impedance are provided for testing the noise level of the setup. Please note that all instruments were thoroughly tested at the factory site before delivery. The suggested tests are mainly intended for optimizing and troubleshooting your setup, to exclude any damage that might have occurred during transportation, or to fulfill your own guidelines, for instance. It will take only a few minutes time and can save time and trouble in the long run. Multi Channel Systems recommends running these tests before you start your real experiments. 1. Connect the terminal plug to one miniature preamplifier and the test model probe to the other as shown in the picture (do not mismatch the polarity). If you have only one MPA32I, you can perform the two tests consecutively. 2. Connect the MPA32I with the test model probe to channels 1 to 32 of the signal collector. 3. Connect the MPA32I with terminal plug to channels 33 to 64 of the signal collector. 4. Make sure that all other connections are appropriate, and that your setup has an appropriate grounding and shielding as recommended in the chapter “General Setup Recommendations”. 5 32-Channel Miniature Preamplifier Manual 4 Setting up MC_Rack Please refer to the MC_Rack Manual for more information. Start MC_Rack. Open the file MPA32I_NoiseTest.rck on the installation volume (see folder Tutorial).This rack contains the virtual MC_Card instrument as data acquisition device with a continuous raw data display and an Analyzer to measure the peak-to-peak amplitude. Click Start to start the recording. — OR — Set up the rack on your own: Click Data Source Setup on the Edit menu. Select a 1-dimensional layout. Select the number of electrode channels (at least 32). Add the data acquisition to your virtual rack. In the tree view pane of the virtual rack, select the data acquisition, and click the Hardware tab. Enter the total amplifier gain according to the specifications of the instruments. For example, for a miniature preamplifier with a gain of 10 and a following filter amplifier with a gain of 100, the total gain is 1000. Select an input voltage range of –819.2 to +818,8 mV and a sampling rate of 10000 Hz. On the Edit menu, click Add Data Display to add a raw data display to your virtual rack. In the virtual rack tree view pane, select the Display 1 and click the Layout tabbed page. Set up a channel map with channels 1 to 64. Adjust the display ranges to 500 ms and +/–50 μV. On the Edit menu, click Add Analyzer to add an Analyzer to the virtual rack. Select all or two typical channels on the Channels tabbed page. Select the Peak-Peak Amplitude parameter on the Analyzer tabbed page. On the Edit menu, click Add Parameter Display to add a Parameter Display to the virtual rack. In the virtual rack tree view, select the Display 2 and click the Layout tabbed page. Set up a channel map with all channels that were assigned to the Analyzer. Click the Ranges tabbed page and enter 0 to 10 s for the x- range and 0 to100 μV for the y- range. 6 FlexMEAs Typical Results Typical results of this test are shown in the following screen shot. The filter amplifier used for this test had a gain of 100 and a bandwidth of 0.5 to 5000 Hz (FA64I-100-0.5-5000). If you use a filter amplifier with a narrower bandwidth, you can generally expect a slightly lower noise level. Noise level test with #MPA8I and FA64I-100-0.5-5000 filter amplifier. The noise level is 60 μV peak to peak with the provided test model probe (channels 1 – 32) and 20 μV peak to peak with grounded inputs (channels 33 – 64). The top window shows raw data; the bottom window shows the extracted peak-to-peak amplitudes of channels 1 and 33. (The miniature preamplifier was shielded by a metal case connected to the ground / earth of the test lab.) 7 32-Channel Miniature Preamplifier Manual 5 Connecting FlexMEAs FlexMEA36, plastic spacer and adapter (ADPT-FM-32) connected to a 32-channel miniature amplifier MPA32I. The side with no screws is considered the top side of the MPA32I. If the adapter is oriented as shown in the figure, the FlexMEA36 is inserted with the contact pads facing downward. For connecting the FlexMEA72 you need the adapter ADPT-FM-72. Please see the datasheet FlexMEA72 in the Appendix. Connecting the FlexMEA36 The FlexMEA adapter has a drawer for inserting the FlexMEA36. Open the slot of the white drawer. Grip the sides of the white slot cover with your fingers and carefully pull open the slot cover only a little bit (about 1 or 2 mm). Do not demount the cover as the plastic supports might break easily. Insert the FlexMEA36 into the slot of the drawer, with the contact pads upside down .Insert the plastic spacer into the slot of the drawer. Important: Please use a thin plastic spacer between the FlexMEA36 and the connector so that the contact pads make a tight contact to the connector pins. Otherwise, the FlexMEA36 will sit too loose and will have a bad contact resulting in a higher noise level. The spacer should be placed on the opposite side of the contact pads of the FlexMEA36 in the drawer. Carefully push back the slot cover of the white drawer until it locks. 8 FlexMEAs FlexMEAs FlexMEAs are made of flexible polyimide material (Polyimide 2611 foil), perfect for in vivo and special in vitro applications. Only 12 μm "thick" and weighing less than 1 g, the FlexMEA biosensor is very thin and lightweight. FlexMEA36 has 32 titanium nitride (TiN) electrodes plus two internal reference electrodes and two ground electrodes. The flexible base is perforated for a better contact with the surrounding tissue. The electrodes have a diameter of about 30 μm with an interelectrode distance of 300 μm. Contact pads and conducting material is pure gold. FlexMEA72 has 72 titanium nitride (TiN) electrodes plus four internal reference electrodes and four ground electrodes. The flexible base is perforated for a better contact with the surrounding tissue. The electrodes have a diameter of about 100 μm with an interelectrode distance from 625 to 750 μm. Contact pads and conducting material is pure gold. Using FlexMEAs FlexMEAs are usually connected to a headstage preamplifier that is connected to a filter amplifier or programmable gain amplifier (see also the ME-System product line of Multi Channel Systems MCS GmbH). FlexMEAs can be via adapter connected to a 32-channel miniature preamplifier MPA32I from Multi Channel Systems for in vivo experiments. FlexMEAs are stable at a temperature range from 10 °C to 40 °C. Warning: Do not autoclave or sterilize FlexMEAs by heat. These MEA types are not heat stable and will irreversibly damaged if the temperature is too high. Do not use ultrasonic bath for cleaning! 9 32-Channel Miniature Preamplifier Manual 5.1 EcoFlexMEAs EcoFlexMEAs are made of flexible polyimide material (Kapton), perfect for in vivo and special in vitro applications. Only 50 μm "thick" and weighing less than 10 g, the EcoFlexMEA biosensor is thin and lightweight. EcoFlexMEA36 has 32 gold electrodes plus two internal reference electrodes and two ground electrodes. The flexible base is perforated for a better contact with the surrounding tissue. The gold electrodes have a diameter of about 50 μm with an interelectrode distance of 300 μm and an impedance of approximately 50 kOhm. Contact pads and conducting material is pure gold. EcoFlexMEA24 has 24 gold electrodes plus two internal reference electrodes and two ground electrodes. The flexible base is perforated for a better contact with the surrounding tissue. The gold electrodes have a diameter of about 80 μm with an interelectrode distance of 300 μm and an impedance of approximately 50 kOhm. Contact pads and conducting material is pure gold. EcoFlexMEAs are usually connected to a headstage preamplifier MPA31I that is connected to a filter amplifier or programmable gain amplifier (see also the ME-System product line of Multi Channel Systems MCS GmbH). EcoFlexMEAs can be directly connected to a 32-channel miniature preamplifier from Multi Channel Systems for in vivo experiments. EcoFlexMEAs are stable at a temperature range from 10 °C to 125 °C and can be autoclaved. 10 FlexMEAs 5.1.1 Connecting 32-Channel NeuroNexus Probes Important: REF has to be connected for obtaining a proper signal. Insert the pin array of the NeuroNexus probe into the black connector of the ADPT-NN-32. Connect the MPA32I to the adapter outputs. Make sure that the orientation of the MPA32I is correct (see illustration). 11 32-Channel Miniature Preamplifier Manual 5.1.2 Adapter-NN-32 Pin Layout The indicated numbers are the ME-System channel numbers that will show up in the MC_Rack program. GND is the ground, REF is the reference input of the miniature preamplifier. Channel Assignment NeuroNexus Probe to MPA32I and MC_Rack The following list shows the assignment to the electrode numbers as given by NeuroNexus Technologies. 12 FlexMEAs 5.2 Connecting 54-Channel NeuroNexus Probes Important: REF has to be connected for obtaining a proper signal. Connect the two MPA32I to the ADPT-NN-54 adapter outputs. Make sure that the orientation of the amplifiers is correct (see illustration). 13 32-Channel Miniature Preamplifier Manual ADPT-NN-54 Pin Layout Numbers in italics are the MPA32I pin numbers. The electrode numbers are aligned to the input pins of the MPA32I. The next figure shows the numbering of the polytrode electrodes. 14 Service and Maintenance 6 Service and Maintenance Cleaning the Connectors Warning: It is recommended to avoid the use of cleaning solutions to avoid corrosion. If a wet cleaning is required, use distilled water. Make sure that only the connectors touch the liquid; do not submerge the miniature preamplifier or the cable. Otherwise, you can fatally damage the electronics. Clean the connectors with 70 % alcohol and cotton swabs from time to time. If this does not provide satisfying results, insert only the connectors into a small beaker with acetone or alcohol and treat them in an ultrasonic bath for 10 to 20 s. Air-dry the miniature preamplifier for about 5 min before use. You can also clean the connectors with distilled water in an ultra sonic bath. Dry the connectors with compressed air immediately after sonication and let the amplifier air dry for at least 6 h before use. Sterilization Warning: Do not autoclave or sterilize miniature preamplifiers by high heat (above 70 °C) or vapor. The resin is not heat-stable and may deform under heat. Vapor can lead to a corrosion of the electronics. Miniature preamplifiers can be sterilized with standard methods that are not based on high heat or vapor, for example, with 70 % ethanol, UV-light, or by thermal sterilization in an oven at 56 °C with an incubation time of 8 hours. 15 32-Channel Miniature Preamplifier Manual 7 Pin Layout 7.1 Power Supply Supply voltage is applied to the output connector pins 19 and 37. The voltage source should supply a stable noise-free voltage. Do not exceed the maximum voltage. Pin 19: –3 V to –8 V Pin 37: +3 V to +8 V Warning: Do not mismatch the polarity of the power supply. A false connection may damage the unit. Input Connector This illustration shows the pin layout viewed from the front, with the case screws upside down. The two reference inputs are used for connecting a reference electrode. Both inputs are equal, so it does not matter which of the two inputs is used. If both are used, the mean of both reference signals is used as reference. Output Connector The signal ground is connected to the ground of the amplifier. The signal ground is used as the reference for the following filter amplifier. 16 Pin Layout 7.2 Test Model Probe Test signal IN: You can use the jumper to connect the signal input pin (Signal IN) to ground (standard situation for a noise level test), or you can connect a data source, for example, a sine wave generator to the input (and the ground of the test signal to the signal ground input) to test the signal distribution. The input signal is distributed across all recording channels. REF I N: The standard situation in an experiment should be that the reference electrode has properties identical or close to the recording electrodes. You can remove the jumper to connect the reference output of the test model probe to the same resistors as the recording channels to mimic the experimental situation, or you can use the jumper to connect the reference output to ground. DC i Dynamic i Capacitanc 4.7 M 330 k 150 pF 17 32-Channel Miniature Preamplifier Manual 8 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. User forum The Multi Channel Systems User Forum provides an excellent opportunity for you to exchange your experience or thoughts with other users worldwide. Mailing list If you have subscribed to the ME-System mailing list, you will be automatically informed about new software releases, upcoming events, and other news on the product line. You can subscribe to the list on the contact form of the MCS web site. www.multichannelsystems.com 18 9 Technical Specifications MPA32I Operating temperature 0° C to 50° C Storage temperature 0° C to 50° C Relative humidity 10 % to 85 % non-condensing Dimensions (W x D x H) 27 x 36 x 5 mm Weight 7 g w/o cable and plug, 56 g with cable and plug Length of the cable 1.5 m Maximum tensile strength of cable 20 N Input connector type Dual-row precision sockets, 50 mil (1.27 mm) grid pattern, for 0.35 to 0.45 mm round pins Supply voltage ± 3 V to ± 8 V DC Supply current < ± 14 mA, typically ± 9 mA Number of input channels 32 Input voltage ± 500 mV (with respect to a supply voltage of 5 V) Input impedance 1012 parallel to 10 pF Input noise < 1.5 μVRMS (1 Hz to 5 kHz, inputs short-circuited) Noise density en = 15 nV / Hz Number of output channels 32 Output voltage = supply voltage Output current max. = 10 mA Output impedance 0 Bandwidth DC to 50 kHz Gain 10 19 32-Channel Miniature Preamplifier Manual 10 Datasheet 20 FlexMEA36 Flexible Microelectrode Array with 36 electrodes for use with 32-Channel Miniature Preamplifier MPA32I-Flex or with the ADPT-FM-32 adapter and the standard MPA32I. 2200 Dimensions in μm 31000 3000 Temperature compartibility Dimension (W x D) Thickness of the electrode field Weight 10 - 40 °C 31 mm x 18.5 mm 12 μm <1g Base material Contact pads and track material Electrode diameter Interelectrode distance (centre to centre) Perforation Polyimide 2611 foil Gold 30 μm 300 μm Diameters of the holes 30 μm Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Number of ground electrodes Planar TiN electrodes (Titanium nitride) Polyimide 2611 foil Approximately 50 k 6x6 32 2 internal reference electrodes 2 MC_Rack Source layout in “Data Source Setup” 5000 Technical Specifications FlexMEA36 350 500 18500 Channel Map Configuration, 64 channels, Amplifier: FA64I/S or FA32I/S, MEA: FlexMEA36 (FlexMEA36.cmp). “Default Map” in Layout tab of Data Display. Cleaning Rinse with distilled water, optional with ethanol 70 %. Do not autoclave or sterilize FlexMEAs by heat. These MEA types are not heat-stable and will be irreversibly damaged! Please do not use ultrasonic bath for cleaning. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. FlexMEA36 Electrode Layout 30 m 300 m GND 5 11 22 28 A2 A3 A4 A5 6 12 21 27 29 B1 B2 B3 B4 B5 B6 3 10 13 20 23 30 C1 C2 C3 C4 C5 C6 2 9 16 17 24 31 D1 D2 D3 D4 D5 D6 1 8 15 18 25 32 E1 E2 E3 E4 E5 E6 REF 7 14 19 26 REF F2 F3 F4 F5 300 m GND 4 The numbers in the electrodes are the recording channel numbers that refer to the channel numbers in the data acquisition program. Please make sure that you have selected “Configuration” in the “Channel Layout” under “Data Source Setup” with a total number of 64 channels. In “Amplifier”, please choose FA32I/S or FA64I/S and in “MEA” FlexMEA36. In Layout tab of the display, please click ”Default Map”. The letter digit code below is the electrode identifier and refers to the position of the electrode in the grid. Important: The channel map is constructed by looking on the back side of the electrodes, because the FlexMEA electrodes are placed on the preparation upside down! If you use more than one MPA32I-Flex and a MEA64-System, the signal collector SC2x32 leads the output channels of the second amplifier to channel number 33 to 64. Please see datasheet SC2x32 for details. The side with the writing NMI is the correct side with the contact pads and electrodes. It might be a bit confusing that the pads look stronger from the wrong side, but if you hold the FlexMEA into the light, you see that the pads have a 3-dimensional appearance only from the correct side. ! Warning: The device may only be used together with the MPA32I (-Flex) from Multi Channel Systems MCS GmbH, and only for the specified purpose. Damage of the device and even injuries can result from improper use. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-FM-32 FlexMEA36 Adapter for 32-Channel Miniature Preamplifiers MPA32I Connecting the FlexMEA36 via Adapter ADPT-FM-32 to the MPA32I. Plastic Spacer FlexMEA36 MPA32I ADPT-FM-32 MPA32I: The side with no screws is considered the top side of the MPA32I. ADPT-FM-32: If the adapter ADPT-FM-32 is oriented as shown in the figure, the FlexMEA36 must be inserted with the contact pads facing downward. Open the white drawer of the adapter. FlexMEA36: Insert the FlexMEA36 carefully into the drawer of the adapter. Please use the plastic spacer to fix the FlexMEA36 in the drawer of the adapter ADPT-FM-32, and to ensure the contact. Plastic Spacer: Insert the plastic spacer carefully into the drawer of the adapter. The plastic spacer should be placed in the opposite side of the contact pads of the FlexMEA36, otherwise the connection will be interrupted. Close the white drawer of the adapter ADPT-FM-32. If you like to use the FlexMEA36 together with the standard 32-channel miniature preamplifier MPA32I, you need the ADPT-FM-32 adapter to connect the FlexMEA36 to the standard MPA32I. There is no need for an adapter if you use the 32-channel miniature preamplifier MPA32I-Flex, which is specified for FlexMEAs. Please read the MPA32I-Flex manual for more information. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. FlexMEA72 Flexible Microelectrode Array with 72 electrodes for use via ADPT-FM-72 adapter with two 32-Channel Miniature Preamplifier MPA32I for in vivo and in vitro applications. Technical Specifications FlexMEA72 Temperature compartibility Dimension (W x D) of the FlexMEA72 Thickness of the electrode field Weight 10 - 40 °C 42 mm x 12 mm 12 μm <1g Base material Contact pads and track material Electrode diameter Interelectrode distance (centre to centre) Perforation Polyimide 2611 foil Gold 100 μm 625 to 750 μm Diameters of the holes 100 μm Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Number of ground electrodes Planar TiN electrodes (Titanium nitride) Polyimide 2611 foil Approximately 50 k 9x8 64 4 internal reference electrodes 4 MC_Rack Data Source Setup Channel map Version 4.1.1 and higher Source Layout: Configuration, Total Number of Channels: 64, Amplifier: FA64I/S, MEA: FlexMEA72. Display: Tab Layout > Default Map (FlexMEA72.cmp). Cleaning Rinse with distilled water, optional with ethanol 70 %. Do not autoclave or sterilize FlexMEAs by heat. These MEA types are not heat-stable and will be irreversibly damaged! Please do not use an ultrasonic bath for cleaning. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. FlexMEA72 625 μm 48 17 57 A1 A2 A3 38 27 47 18 56 B1 B2 B3 28 37 46 55 64 C1 C2 C3 36 45 D1 8 17 26 A6 A7 A8 9 18 27 B6 B7 B8 1 10 19 28 C4 C5 C6 C7 C8 54 63 2 11 20 29 D2 D3 D4 D5 D6 D7 D8 35 44 53 62 3 12 21 30 E1 E2 E3 E4 E5 E6 E7 E8 34 43 52 58 7 13 22 31 F1 F2 F3 F4 F5 F6 F7 F8 33 42 49 59 6 16 23 32 G1 G2 G3 G4 G5 G6 G7 G8 REF 40 50 60 5 15 25 REF H2 H3 H4 H5 H6 H7 41 51 61 4 14 24 J2 J3 J4 J5 J6 J7 GND GND REF MPA32I Channel 33 - 64 GND REF GND 750 μm 100 μm Electrode field Direction to contact pads 39 26 MPA32I Channel 1 - 32 The numbers in the electrodes are the recording channel numbers that refer to the channel numbers in the data acquisition program. Please make sure that you have selected “Configuration” in the “Channel Layout” under “Data Source Setup” with a total number of 64 channels. In “Amplifier”, please choose FA64I/S and in “MEA” FlexMEA72. In Layout tab of the display, please click ”Default Map”. The letter digit code below is the electrode identifier and refers to the position of the electrode in the grid. Important: The MC_Rack channel map is constructed by looking on the back side of the electrodes, because the FlexMEA electrodes are placed on the preparation upside down! Please make sure to connect the left hand side of the FlexMEA72 contact pads (electrode side on top) via ADPT-FM-72 adapter to the first MPA32I (channels 33 to 64), and the right hand side to the second MPA32I (channels 1 to 32). Read the SC2x32 data sheet when using a signal collector. The electrodes are on the same side as the contact pads. The side with the writing “NMI” is the side with the contact pads and electrodes. It might be a bit confusing that the pads look “stronger” from the wrong side, but if you hold the FlexMEA72 into the light, you see that the contact pads have a three-dimensional appearance only from the correct side. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-FM-72 FlexMEA adapter for connecting two MPA32Is to the FlexMEA72 Connector for FlexMEA72 GND Connector ADPT-FM-72 Connector for FlexMEA72 Connector for MPA32I No. 2 Connectors for FlexMEA72 Connector for MPA32I No. 1 Plastic Spacer Adapter ADPT-FM-72 connected to both MPA32Is. FlexMEA72 ADPT-FM-72: The adapter is equipped with the connectors for the MPA32Is on one side and the connectors for the FlexMEA72 on the other side. MPA32I: The side with no screws is considered to be the top side of the MPA32I. The MPA32Is have to be connected with their top sides facing to each other (to the inner part of the adapter) and the bottom sides facing outwards. FlexMEA72: On the FlexMEA72 the side with the writing “NMI” is the side with contact pads and electrodes. This side is optically golden shimmery and the letters “NMI” are readable. Plastic Spacer: The spacer between FlexMEA72 and drawers ensures the contact from the printed circuit board of the adapter and the contact pads. Warning: The adapter ADPT-FM-72 may only be used together with a FlexMEA72 and with MPA32I from Multi Channel Systems MCS GmbH, and only for the specific purpose. Damage of the device and even injuries can result from improper use. Scope of delivery: 1 FlexMEA72 adapter ADPT-FM-72 6 Button head srews M2x5 mm, washers, screw nuts 2 Bars Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 250 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-FM-72 FlexMEA adapter for connecting two MPA32Is to the FlexMEA72 Please follow the instructions for mounting the the FlexMEA72 on the ADPT-FM-72 and the MPA32Is step by step. Necessary tools: A small Philipps head screwdriver a sharp forceps 1. Please loosen the screws of the adapter that it is possible to move the bars of the ADPT-FM-72. 2. Open the withe drawers of the adapter by pre-drawing the black parts of the drawers with the help of a forceps. 3. Connect the MPA32Is in correct manner, the bottom sides with screws must face outwards. Drawer Spacer Contact pads of FlexMEA Adapter with PBC 4. Insert the contact pads of the FlexMEA72 carefully with a forceps into the first drawer. Insert the FlexMEA72 upside down, because the contact pads must have contact to the printed circuit board of the ADPT-FM-72. Place the plastic spacer between contact pads and drawer for a good contact between PCB and FlexMEA72. Please see the scheme. The electrode part of the FlexMEA should face in direction of the ground connector. Please handle the FlexMEA72 with great care! 5. Close the first drawer by pushing back the black part into the white part. The contact pad is fixed. 6. Insert the opposite contact pad of the FlexMEA72 into the second drawer, place the plastic spacer between the back side of the FlexMEA and the drawer and close it. 7. Thighten the screws of the ADPT-FM-72. : When connecting the FlexMEA72 and the adapter as shown on the picutre, the electrodes of the FlexMEA72 face in the same direction as the MPA32Is. The MPA32I on the right side is considered to be MPA32I No 1 with channels No 1 to 32. The MPA32I on the left side of the FlexMEA is defined to be MPA32I No 2 with channels 33 to 64. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 250 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-NN-32 32-Electrode NeuroNexus Probe Adapter for MPA32I Amplifiers Connecting the NeuroNexus probe and the MPA32I Warning: The adapter may only be used together with the MPA32I from Multi Channel Systems MCS GmbH and the 32-channel probe from NeuroNexus, and only for the specified purpose. Damage of the device and even fatal injuries can result from improper use. ! The side with no screws is considered the top side of the MPA32I. Please connect the MPA32I with the top side up to the bottom side of the adapter as shown in the picture beside. Side view NeuroNexus probe Bottom ADPT-NN-32 Input pins MPA32I Top Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-NN-32 32-Electrode NeuroNexus Probe Adapter for MPA32I Amplifiers Pin layout of the input connector Pin assignment The indicated numbers are the ME-System channel numbers that will show up in the MC_Rack program. The list on the second page shows you the assignment to the electrode numbers as given by NeuroNexus Technologies. GND is the ground, REF is the reference input of the miniature preamplifier. Please see the MPA32I manual for details. Optionally, it is possible to connect G1/G2 and GND together. MPA32I to 32-electrode NeuroNexus Probe Important: Operation of the MPA32I is differential. REF has to be connected for obtaining a proper signal. Please see also the MPA32I manual for more details. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany MPA32I MC_Rack Pin 1: Pin 2: Pin 3: Pin 4: Pin 5: Pin 6: Pin 7: Pin 8: Pin 9: Pin 10: Pin 11: Pin 12: Pin 13: Pin 14: Pin 15: Pin 16: Pin 17: Pin 18: Pin 19: Pin 20: Pin 21: Pin 22: Pin 23: Pin 24: Pin 25: Pin 26: Pin 27: Pin 28: Pin 29: Pin 30: Pin 31: Pin 32: Pin 33: Pin 34: Pin 35: Pin 36: (GND) REF Ch. 1 Ch. 2 Ch. 3 Ch. 4 Ch. 5 Ch. 6 Ch. 7 Ch. 8 Ch. 9 Ch. 10 Ch. 11 Ch. 12 Ch. 13 Ch. 14 Ch. 15 Ch. 16 Ch. 17 Ch. 18 Ch. 19 Ch. 20 Ch. 21 Ch. 22 Ch. 23 Ch. 24 Ch. 25 Ch. 26 Ch. 27 Ch. 28 Ch. 29 Ch. 30 Ch. 31 Ch. 32 REF (GND) Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. Probe Rev 3 Probe Rev 2 REF El. 16 El. 12 El. 15 El. 10 El. 14 El. 8 El. 13 El. 6 El. 4 El. 2 El. 1 El. 3 El. 5 El. 7 El. 9 El. 11 El. 32 El. 30 El. 31 El. 28 El. 29 El. 26 El. 27 El. 24 El. 25 El. 20 El. 22 El. 19 El. 23 El. 18 El. 21 El. 17 REF REF El. 16 El. 12 El. 15 El. 11 El. 14 El. 10 El. 13 El. 9 El. 8 El. 7 El. 6 El. 5 El. 4 El. 3 El. 2 El. 1 El. 32 El. 31 El. 30 El. 29 El. 28 El. 27 El. 26 El. 25 El. 24 El. 20 El. 23 El. 19 El. 22 El. 18 El. 21 El. 17 REF ADPT-NN-54 54-Electrode NeuroNexus Probe Adapter for MPA32I Amplifiers Connecting two MPA32I to the ADPT-NN-54 Complete setup with two MPA32Is IN/OUT 1 IN/OUT 2 Top view MPA32I No. 1 MPA32I No. 2 The IN / OUT sockets 1 and 2 can be used as output connectors, for connecting single channels to other devices, or as input connectors, for connecting other signal sources. Side view Connect MPA32I No. 1 here Top Bottom Connect MPA32I No. 2 here Important: Connect both MPA32I with the screws face down as shown in the picture above. Otherwise, the miniature preamplifiers will not work. The side with no screws is considered to be to top side of the MPA32I ! Warning: The device may only be used together with the MPA32I from Multi Channel Systems MCS GmbH and the 54-channel probe from NeuroNexus, and only for the specified purpose. Damage of the device and even fatal injuries can result from improper use. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-NN-54 54-Electrode NeuroNexus Probe Adapter for MPA32I Amplifiers Pin Layout Adapter to 54-electrode NeuroNexus probe MPA32I No. 1, IN / OUT 1 1 3 5 GND REF 2 4 7 9 11 13 15 17 19 21 23 25 27 29 31 12 10 8 6 4 2 27 25 23 15 17 19 21 33 35 REF 13 11 9 7 5 3 1 26 24 14 16 18 20 22 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 GND 33 36 MPA32I No. 2, IN / OUT 2 1 3 GND 5 7 9 11 13 15 17 19 21 23 25 27 29 31 28 29 31 41 39 37 35 33 43 45 47 49 51 53 30 32 40 38 36 34 42 44 46 48 50 52 54 8 10 12 14 16 18 20 22 24 26 28 30 32 REF 2 4 6 35 REF GND 34 36 Numbers in italics are the MPA32I pin numbers. The electrode numbers are aligned to the input pins of the MPA32I. Polytrode: electrode numbers 23 24 25 26 27 28 29 30 31 32 22 33 21 34 20 35 19 36 18 37 17 38 16 39 15 40 14 41 13 42 12 43 11 44 10 45 9 46 8 47 7 48 6 49 5 50 4 51 3 52 2 53 1 54 32-Channel Miniature Preamplifier (MPA32I) inputs 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 Pin 1: Pin 2: Pin 3 - 34: Pin 35: Pin 36: GND (ground) REF (reference) Channel 1 to 32 REF (reference) GND (ground) Important: Operation of the MPA32I is differential. REF has to be connected for obtaining a proper signal. Please see the MPA32I manual for details. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-NN-64 Adapter for connecting two MPA32Is to the Acute 64 Probe from NeuroNexus Technology ADPT-NN-64 ADPT-NN-64 MPA32I No. 1 MPA32I No. 2 Acute 64 Probe Electrodes Acute 64 Probe Schematic diagram : Connect the male pins at the bottom side of the Acute 64 Probe to the female pins at the bottom side of the adapter ADPT-NN-64. Connect the upper output pins at the top side of the adapter ADPT-NN-64 to the MPA32I No. 1. It is mandatory, that the top side of the MPA32I No. 1 faces upwards. (The side with no screws is considered the top side of the MPA32I). Connect the lower output pins at the bottom side of the adapter ADPT-NN-64 to the MPA32I No. 2. The top side of the MPA32I No. 2 must face upwards to the bottom side of the MPA32I No.1, otherwise the preamplifiers will not work. Top Side MPA32I No. 1 Adapter Top Side ADPT-NN-64 1 2 Adapter Bottom Side °° PCB Top View (Probe on the bottom side) 2 1 Top Side 1 MPA32I No. 2 ! 1 2 Direction of the -----> Electrodes °° 2 Acute 64 Probe (NeuroNexus Technology) Warning: The ADPT-NN-64 may only be used together with the MPA32I from Multi Channel Systems MCS GmbH, and only for the specified purpose. Damage of the device and even injuries can result from improper use. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. ADPT-NN-64 Adapter for Acute 64 Probe from NeuroNexus Technology Acute 64 Probe Site Mapping (Electrode Layout) Pin Layout : MC_Rack MPA32I No. 1 and No. 2 Electrodes of Acute 64 Probe REF 5 13 21 29 37 45 53 61 4 12 20 28 36 44 52 60 6 14 22 30 38 46 54 62 3 11 19 27 35 43 51 59 1 7 15 23 31 39 47 55 63 63 2 2 10 18 26 34 42 50 58 62 3 8 16 24 32 40 48 56 64 61 4 1 9 17 25 33 41 49 57 60 59 6 5 58 57 8 7 56 55 10 9 PCB Top View (Probe on the bottom side) 64 GND GND 54 53 12 11 52 51 14 13 50 49 16 15 48 47 17 REF REF 18 46 19 45 20 44 43 22 21 42 37 28 23 41 33 32 24 40 36 29 25 39 35 30 26 38 34 31 27 Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany MC_Rack MPA32I No.1 Electrode 1 GND 2 REF CH 1 3 49 CH 2 4 50 CH 3 5 51 CH 4 6 52 CH 5 7 53 CH 6 8 54 CH 7 9 55 CH 8 10 56 CH 9 11 57 CH 10 12 58 Ch 11 13 59 CH 12 14 60 CH 13 15 61 CH 14 16 62 CH 15 17 63 CH 16 18 64 CH 17 19 1 CH 18 20 2 CH 19 21 3 CH 20 22 4 CH 21 23 5 CH 22 24 6 CH 23 25 7 CH 24 26 8 CH 25 27 9 CH 26 28 10 CH 27 29 11 CH 28 30 12 CH 29 31 13 CH 30 32 14 CH 31 33 15 CH 32 34 16 35 REF 36 GND MC_Rack MPA32I No. 2 1 2 CH 33 3 CH 34 4 CH 35 5 CH 36 6 CH 37 7 CH 38 8 CH 39 9 CH 40 10 CH 41 11 CH 42 12 CH 43 13 CH 44 14 CH 45 15 CH 46 16 CH 47 17 CH 48 18 CH 49 19 CH 50 20 CH 51 21 CH 52 22 CH 53 23 CH 54 24 CH 55 25 CH 56 26 CH 57 27 CH 58 28 CH 59 29 CH 60 30 CH 61 31 CH 62 32 CH 83 33 CH 64 34 35 36 Fon +49-7121-9 09 25- 0 Fax +49-7121-9 09 25-11 © 2014 Multi Channel Systems MCS GmbH [email protected] www.multichannelsystems.com Product information is subject to change without notice. Electrode GND REF 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 REF GND
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