TBSI M Series System’s Manual Version 2.0 Triangle BioSystems International 2224 Page Rd. Suite 108 Durham, NC 27703 Phone: (919) 361‐2663 • Fax: (919) 544‐3061 www.trianglebiosystems.com Multiplexer System User’s Manual Contents
32 CHANNEL MULTIPLEXING HEADSTAGE SYSTEMS ............................................................................... 3 Introduction ............................................................................................................................................. 3 Features ................................................................................................................................................... 3 System Configuration ............................................................................................................................... 4 Headstage Transmistter Specifications .................................................................................................... 4 Multiplexer System Parts List .................................................................................................................. 5 Headstage Transmitter Specifications ..................................................................................................... 6 Headstage Mechanical Overview ............................................................................................................ 7 16 Channel Headstage Pinout .................................................................................................................. 8 RF Receiver Specifications ....................................................................................................................... 9 RF Receiver Mechanical Overview ......................................................................................................... 10 RF Receiver Signal Interface Options ..................................................................................................... 10 Receiver Signals ‐ Analog Output ........................................................................................................... 11 Receiver Signals– Digital Interface ......................................................................................................... 12 System Setup and Testing ...................................................................................................................... 13 Headstage Accessory Options ................................................................................................................ 14 LED Headstage Options .......................................................................................................................... 15 Multiple Headstages and demux recievers ........................................................................................... 15 Commutators ......................................................................................................................................... 16 Gain and Phase Response ...................................................................................................................... 16 Troubleshooting ..................................................................................................................................... 17 Application Notes ................................................................................................................................... 17 Contact Us .............................................................................................................................................. 17 Version3.0
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8/2014 Multiplexer System User’s Manual 32CHANNELMULTIPLEXINGHEADSTAGESYSTEMS
Introduction
Triangle BioSystems International (TBSI) offers a family of multiplexing channel analog multiplexing headstage subassemblies that are used to provide a reduced wired connection between implanted electrodes and neural recording and analysis equipment. The main function of the headstage is to precondition the neuron pulse signals and provide a high gain, bandpass filtered buffered connection over a 3 wire cable. Each headstage design is based on a custom, low power VLSI developed by TBSI. The result is a solution with superior performance and reduced wire connection in a very small form‐factor with less weight. The 5ch, 16ch and 32 channel high gain, bandpass filter headstages are available with system gain of 800 with an adjustable bandpass filtering from the factory. Features
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3 wire interface cable for 5, 15 and 31 channels
with 5 feet standard length 
32ch: Size:5x15x20 mm @ 0.8 grams
16ch: Size:4x14x14 mm @ 0.6 grams
5ch: Size:4x14x 6 mm @ 0.5 grams 
Also available with multiple headstage options for
62, 96, 128, 160, 192, 224 channels with 4, 5, 6, 7,
8, 9 wired cables respectively. 
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Available with system gain of 800 Headstage Bandpass filtering of .8Hz to 7khz Optional Blue, Red and Green LED available for
video tracking Version3.0
Multiplex Receiver
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8/2014 Multiplexer System User’s Manual SystemConfiguration
See the section entitled System Setup and Testing for more details on proper system assembly and positioning. TBSI Multiplexing Recording Setup HeadstageTransmistterSpecifications
The multiplexing neural headstage system consists of a tethered headstage, an RF receiver and baseband demultiplexer subsystems as shown below: NOTE: These products are not intended for human use. Version3.0
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8/2014 Multiplexer System User’s Manual MultiplexerSystemPartsList
Multiplexer System Parts List Item # Part Number 1 Various 2 Various 3 Various 4 Various 5 100‐0000‐11 6 202‐0001‐10 7 008‐0012‐01 Version3.0
Description Headstage Commutator Receiver Multiplexer Test Cable Receiver 6 VDC power supply 120 VAC/240 UL and FCC approved DB37 Connector M 64 Channel Shorting Plug ‐5‐
8/2014 Multiplexer System User’s Manual HeadstageSpecifications
PARAMETER Power Supply Headstage Pwr supply Average I cc 3.0v Analog Channel Max Input voltage range Max Output Voltage range Common mode center Dc Offset System Gain 120 System Gain 600 Bandwidth @ 3v Input impedance Output impedance MIN TYP MAX UNITS NOTES 2.99 3.0 3.01 Volts
5.6 6.1 6.7 ma
3.0 Bipolar power (+2.1v, ‐.9v and ACgnd @ 0V) Without LEDs +2 mVolts For gain of 800 System (headstage gain of 100) +1.2 Volts ‐2 ‐1.2 ‐10 115 790 .8 Input referred noise Volts 10 125 810 7000 Hz Mohms Ohms
8.5 µVrms 5.5 50 ‐63 µVrms 15 15 11 30 20 16 12 5 5 5 Maximum Weight .8 Version3.0
mVolts Input referred noise Channel sample Rate THD Phase Delay Headstage Mechanical Length, Width, Height Length, Width, Height Length, Width, Height 0 0 120 800 11 158 kHz dB µSecs mm mm mm
Grams Measured at DB37 connector of receiver ACgnd @ 0v For bigpolar power supplies only Factory selectable gain Factory selectable gain ‐3dB input signal level BW At 1kHz At 1khz For DC – 10khz frequency with all inputs grounded For 500Hz – 5Hz frequency with all inputs grounded Perchannel sampling rate @5kHz and 1 volt p‐p input @ 5 kHz input Dimensions of 32ch headstage board only
Dimensions of 16ch headstage board only
Dimensions of 5ch headstage board only Headstage only (wire not included in weight) ‐6‐
8/2014 Multiplexer System User’s Manual HeadstageMechanicalOverview
Top View
Bottom View
Complete headstage weight is 2.7 g (single board) or 3.4 grams (dual board, standard version) 32ChannelHeadstagePinout
.025” Omnetics Female TBSI P/N: A9409 Mating Male Electrode Connector TBSI P/N: A9707 Pin # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Version3.0
Pin Connection GND Channel 30 Channel 28 Channel 26 Channel 24 Channel 22 Channel 20 Channel 18 Channel 16 Channel 14 Channel 12 Channel 10 Channel 8 Channel 6 Channel 4 Channel 2 Channel NC GND ‐7‐
Pin # 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 35 Pin Connection NC Channel 31 Channel 29 Channel 27 Channel 25 Channel 23 Channel 21 Channel 19 Channel 17 Channel 15 Channel 13 Channel 11 Channel 9 Channel 7 Channel 5 Channel 3 Channel 1 GND GND 8/2014 Multiplexer System User’s Manual 16ChannelHeadstagePinout
.025” Omnetics Female TBSI P/N: A9094 Mating Male Connector TBSI P/N: A7874 Version3.0
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8/2014 Multiplexer System User’s Manual .025” Omnetics Female TBSI P/N: A70008 Mating Male Connector TBSI P/N: A70009 DemultiplexerReceiverSpecifications
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Input referred noise, typical: 4 µV RMS Input voltage range: +/‐ 0.5 V Analog channel bandwidth: 20 kHz DC offset: < 100 µVdc Phase delay typical: 30 µsec at 10 kHz Signal lock indicator: LED on front panel Version3.0
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8/2014 Multiplexer System User’s Manual DemultiplexerReceiverMechanicalOverview
DemultiplexerReceiverSignalInterfaceOptions
The W5 RF Receiver Base Station is available in many different system configurations to accommodate a wide range of data acquisition system interface requirements. The RF receiver signal is demodulated by a custom digital signal processing system that incorporates a high speed analog to digital converter for demodulating the receiver signal. The demodulated signal is then conditioned via the DSP and converted to an analog signal. The analog signal can be directly output to one or two DB‐37 connectors, input to an integrated Analog to Digital Converter (ADC) or both. Version3.0
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8/2014 Multiplexer System User’s Manual ReceiverSignals‐AnalogOutput
The bottom DB37 connector is
for the single 32 channel headstage
and the top and bottom DB37
connector is used with the dual, 64
channel headstages. The 96 channel
and 128 channel use 4, DB37
connectors or 1 DB37 connector for
each 32 channel headstage all with the
same pinout characteristics.
In addition, please do not short
No Connects at DB37 together since
these may have some test signals
placed on them. Version3.0
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Pin # Description 1 GND 2 Channel 30 Output 3 Channel 28 Output 4 Channel 26 Output 5 Channel 24 Output 6 Channel 22 Output 7 Channel 20 Output 8 Channel 18 Output 9 Channel 16 Output 10 Channel 14 Output 11 Channel 12 Output 12 Channel 10 Output 13 Channel 8 Output 14 Channel 6 Output 15 Channel 4 Output 16 Channel 2 Output 17 No Connect 18 GND 19 No Connect 20 Channel 31 Output 21 Channel 29 Output 22 Channel 27 Output 23 Channel 25 Output 24 Channel 23 Output 25 Channel 21 Output 26 Channel 19 Output 27 Channel 17 Output 28 Channel 15 Output 29 Channel 13 Output 30 Channel 11 Output 31 Channel 9 Output 32 Channel 7 Output 33 Channel 5 Output 34 Channel 3 Output 35 Channel 1 Output 36,37 GND 8/2014 Multiplexer System User’s Manual ReceiverSignals–DigitalInterface
TBSI offers an internal DAQ board option and NeuroWare™ software for the 5ch, 16ch, 32ch and 64ch receivers which can be used for signal data acquisition and analysis. Digital output and event input information is sent to the PC via a USB connection. The total system gain for the digital receiver is 800. The integrated data acquisition card can also be read using DAQ toolkits from MatLab® and Labview®. Version3.0
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8/2014 Multiplexer System User’s Manual SystemSetupandTesting
The steps below should be followed when setting up your Multiplex system to optimize its performance. 1) Connect power supply to de‐multiplexer receiver Plug the DC end of the power supply cable into the back of receiver and the other end into an AC outlet. The recording system is powered by an AC line adapter transformer/regulator. This power unit connects to a 100‐240 VAC, at 47‐63 Hz and is rated at 6 VDC at 2.5 A power source and is UL approved. Use a suitable International adapter to mate the standard US plug to your AC outlet. 2) Connect signal cable to receiver analog output DB37 connector If your system was configured for analog system output, connect a suitable analog output cable to the DB37 receiver connector. Five signal wires and a ground wire can be added to the DB37 mating connector to check for signal output. The lengths of the wires are not critical. The analog output channel positions are described in the section entitled Receiver Signal Interface – Analog Output. If your receiver has an integrated DAQ, plug the USB cord into the receiver and PC and follow the instructions provided in the Neuroware Manual or your data acquisition software for signal acquisition and viewing. 3) Turn on Receiver Flip the on/off button of the receiver to control power to the receiver. The button will light up green when the receiver is turned on and the light will go off when it is off. Version3.0
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8/2014 Multiplexer System User’s Manual 4) Connectafunctiongeneratortotheheadstageviaheadstagesignalcable Once the receiver and headstage (see #7 below) have both been switched on, the “Signal Lock” LED on the receiver front panel should light up. After confirming the signal lock integrity, you can view the analog signals (output from the DB37 connector on the back of the receiver) with an oscilloscope. Please note the default system gain is 800, therefore you can expect the analog output values to be about 800 Vp‐p of the signal from the function generator. HeadstageAccessoryOptions
Three biosensor options are available as add‐ons for any wireless headstage. These accessories replace channels typically used for neural recording on the headstage. Installation of any of these features increases the headstage length by 0.4 inches, its height by 0.2 inches, and its weight by 0.4 grams. The features can be installed individually or together in any combination. See the TBSI Biosensors Brochure for more information. Due to the fact that sensor options require 1 or 3 data channels each, these may not be practical for use with the W5 headstage; consider a W16 or higher headstage. Accelerometer
This add‐on will monitor x, y, and z acceleration vectors and output the information via three analog signal channels. The animal head position and velocity can later be calculated with software such as Matlab. UltrasonicMicrophone
This single ultrasonic microphone enables recording of audio frequencies between 1 kHz and 25 kHz (W5 and W32 Headstages) or 1‐40 kHz (W16 and W64 Headstages) and uses one recording channel. TemperatureSensor
A Thermistor based temperature sensor option is available. Many sensor sizes are available including very small ones that can be implanted within or near the brain. The sensor must be attached to a mating connector and externalized to the head of the animal much like traditional recording electrodes. Sensors are customized to your particular research needs. Version3.0
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8/2014 Multiplexer System User’s Manual LEDHeadstageOptions
Red and blue LED options are available for the 32 channel headstage for video tracking. The
LEDs are placed facing upward on the top headstage and plenty bright enough to allow for
video tracking. Both LEDs turn on with the On/Off switch.
MultipleHeadstagesanddemuxrecievers
The 5, 16, 32, 64, 96 and 128 channel M-series are shown below:
The 5, 16 and 32ch demux receivers have a DB37 for analog output connectors. The 64ch demux receivers have
2, DB37 (A, B) for analog output connectors. The bottom DB37 connector (A) is for the 32ch headstage and
connector A and B is for the 64ch headstage.
The 96ch and 128ch demux receivers are taller and have 4, DB37 connectors (A, B, C, D) for analog output
connections. Connector A,B,C are used of the 96ch outputs and A,B,C, and D are used for the 128ch outputs.
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8/2014 Multiplexer System User’s Manual Commutators
These are some of the commutator options that are available which are sold with our multiplexer system
separately.
The Dragonfly SL-4c, SL-6c and SL-10c and Plastics One 4ch swivel are shown below.
GainandPhaseResponse
Please refer to the section of this document entitled “Headstage Transmitter Specifications” for numerical data on bandwidth and input referred noise. Version3.0
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8/2014 Multiplexer System User’s Manual Troubleshooting
Problem: No neural signals are visible on any of the analog outputs at the DB37 connector. Suggestion: Verify the AC power connection is in place and the green “Power” LED is illuminated on the front of the RF receiver box. Also, verify that the yellow “Signal Lock” light is illuminated, which confirms that the receiver is receiving the signal transmitted from the headstage. Problem: Visible neural signal is missing information. Suggestion: Keep the animal within the 4 meter range of the receiver. If you exceed this range, the radio signal from the headstage will not be strong enough to maintain reliable signal monitoring of the animal. Also, be sure to keep the area under the RF receiver unit’s antennas free from metallic objects, which will reduce signal range and introduce noise. Problem: Not all channels are visible on the neural signal. Suggestion: Make sure the headstage connection to the animal is secure. ApplicationNotes
1) The wireless headstage is a low‐power device, it is critical that the RF receiver be carefully located for the system to operate. DO NOT place the receiver on the outside of a metal wire cage. Try to minimize the distance between the receiver and the wireless headstage. Please refer to the section in this document entitled System Setup and Testing for orientation suggestions. 2) If you do not intend to record from all of the available signal channels you must ground any unused channels at the electrode connector or EIB interface. Failure to do so can create artifact noise from the floating channels on the other signal channels. ContactUs
Triangle BioSystems International 2224 Page Rd. Suite 108 Durham, NC 27703 Phone: (919) 361‐2663 • Fax: (919) 544‐3061 www.trianglebiosystems.com Need more help? You can reach TBSI customer support by phone at the number listed above (M‐F Eastern US Time) or email us at [email protected]. If you need replacement parts or accessories for your system or to learn the latest about available TBSI products visit our website, call or email us at [email protected]. Version3.0
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8/2014