Technical Program AGENDA OF SESSIONS October 04 – October 08, 2014 SATURDAY, October 04: At SERHS Natal Grand Hotel, Room Água Marinha 8:30 – 18:00 Registration (Foyer) Pre-Symposium Course SESSION A. Fundamentals 9:00 – 9:50 Fundaments of Capillary Electrophoresis Norberto A. Guzman, U.S.A. 9:50 – 10:40 Computer Optimization of Electrolytes for Capillary Electrophoresis Bohuslav Gaš, Czech Republic 10:40 – 11:10 Coffee Break 11:10 – 12:00 Microchip Technology Vincent T. Remcho, U.S.A. 12:00 – 12:50 Fundaments of Liquid Chromatography Attila Felinger, Hungary 13:00 – 14:00 Lunch Recess (Restaurant Potiguar – 2nd floor) SESSION B. Hyphenation 14:00 – 14:50 CE-MS Technology Christian W. Klampfl, Austria 14:50 – 15:40 Introduction to CE(-MS) for Peptide and Protein Analysis Govert W. Somsen, The Netherlands 15:40 – 16:00 Coffee Break 16:00 – 18:00 AB Sciex workshop SUNDAY, October 05: At SERHS Natal Grand Hotel, Room Água Marinha Pre-Symposium Course SESSION C. Omics 9:00 – 9:50 Introduction to Metabolomics Fco. Javier Rupérez & Carolina Simó, Spain 9:50 – 10:40 Introduction to Glycomics Yehia Mechref, U.S.A. 10:40 – 11:00 Coffee Break 11:00 – 13:00 Agilent Technologies workshop The fish and the bird… Where we are and where we are going to with Mass Spec Technology. Fundamentals and Some Applications. Alana Figueiredo and Jose Xavier – Mass Spec Product Specialists 13:00 – 14:00 Lunch Recess (Restaurant Potiguar – 2nd floor) OPENING SESSION At SERHS Natal Grand Hotel, Room Água Marinha 14:00 – 17:00 Registration (Foyer) and Poster Set-up (all posters) 17:00 – 17:15 Welcoming Remarks Marina F.M. Tavares, University of Sao Paulo, Brazil Emanuel Carrilho, University of Sao Paulo, Brazil Edgar P. Moraes, Federal University of Rio Grande do Norte, Brazil PLENARY LECTURES I Chairperson, Dr. Marina F.M. Tavares 17:15 – 17:55 PL-A-01 Electrophoretic separation of single molecules using thermoplastic nanocolumns Steven A. Soper, U.S.A. 18:00 – 18:40 PL-A-02 Single molecule immunoassay at femto liter on extended-nano fluidic device: a crazy ELISA Takehiko Kitamori, Japan 18:45 – 19:25 PL-A-03 Glycomic profiling in cancer: combined information from mass spectrometry and microchip CE Milos V. Novotny, U.S.A. 19:30 – 20:30 Cocktails (Varanda Bossa Nova) MONDAY, October 6: 8:00 – 18:00 Registration (Foyer) and Poster Set-up (all posters) Room Água Marinha Room Europa SESSION I – Proteins & Peptides SESSION II - Fundamentals I Chairperson, Dr. Claudimir L. do Lago Chairperson, Dr. Gustavo A. Micke 8:30 – 9:00 KN-A-01 Capillary electrophoresis and isotachophoresis employed for physicochemical characterization of peptides Václav Kašička, Czech Republic 8:30 – 9:00 KN-A-02 9:00 – 9:20 OP-A-01 Characterizing intact proteins and 9:00 – 9:20 OP-A-05 protein complexes using native conditions with CESI-MS Jeff D. Chapman, U.S.A. Fate of one equation Bohuslav Gaš, Czech Republic 9:20 – 9:40 OP-A-02 Middle-up characterization of mAbs and ADCs by capillary electrophoresis-mass spectrometry Govert W. Somsen, The Netherlands 9:20 – 9:40 OP-A-06 Internal standard capillary electrophoresis as a high throughput method for pKa determination in drug discovery and development Martí Rosés, Spain 9:40 – 10:00 OP-A-03 Use of capillary electrophoresis for monitoring iron-peptide complex formation Nora M. Vizioli, Argentina 9:40 – 10:00 OP-A-07 MEKC selectivity revisited: is localization important? Marina F.M. Tavares, Brazil 10:00 – 10:20 OP-A-04 Use of monolithic supports for isolation of human plasma derived and recombinant therapeutic proteins Djuro Josic, U.S.A. & Croatia 10:00 – 10:20 OP-A-08 Fast liquid chromatography – problems and solutions Attila Felinger, Hungary 10:20 – 11:20 Effect of the counter ion in background electrolyte on electrokinetic injection / electrokinetic supercharging – CZE analysis of diluted samples Takeshi Hirokawa, Japan Coffee Break, and Vendor Exposition POSTER PRESENTATION I (PP01 – PP10; PP31 – PP40; PP61 – PP70) Room Água Marinha Room Água Marinha Room Europa SESSION III – Metabolomics SESSION IV - Instrumentation Chairperson, Dr. Edgar P. Moraes Chairperson, Dr. Dosil P. de Jesus 11:30 – 12:00 KN-A-03 11:30 – 12:00 KN-A-04 Multiplatform metabolomics for investigation of adverse outcomes in Stable Acute Coronary Syndrome (SCAD) Fco. Javier Rupérez, Spain Photonic crystals for chip and capillary electrophoresis Yi Chen, China 12:00 – 12:20 OP-A-09 Metabolomics of plasma samples in study of resistant hypertension by use of LC/TOF/MS and GC/QqQ/MS techniques Michal Markuszewski, Poland 12:00 – 12:20 OP-A-13 What’s new on CE/LedIF ? François Couderc, France 12:20 – 12:40 OP-A-10 Sheathless capillary electrophoresis-mass spectrometry: an enabling technology for small-volume metabolomics studies Rawi Ramautar, The Netherlands 12:20 – 12:40 OP-A-14 Development and applications of sequential injection – capillary electrophoresis systems Michael C. Breadmore, Australia 12:40 – 13:00 OP-A-11 12:40 – 13:00 Metabolite profiling by CE-MS. A study of the effects of bioactive OP-A-15 food ingredients on human colon cancer cells Carolina Simó, Spain 13:00 – 13:20 OP-A-12 The role of CE/CE-MS in metabolomics Silas G. Villas-Bôas, New Zealand 13:30 – 14:30 13:00 – 13:20 OP-A-16 Free liquid membranes – a novel progressive concept of phase interfaces for electrically enhanced microextraction techniques Pavel Kubáň, Czech Republic New high speed and high resolution gradient techniques Mark A. Hayes, U.S.A. Lunch Recess (Restaurant Potiguar – 2nd floor) Room Água Marinha Room Europa SESSION V – Microfluidics & Microchip I SESSION VI – New Applications I Chairperson, Dr. José Alberto Fracassi da Silva Chairperson, Dr. Lucas Blanes 14:30 – 15:00 KN-A-05 Sensitive analysis of environmental pollutants by microchip electrophoresis with contact conductivity detection Charles Henry, U.S.A. 14:30 – 15:00 KN-A-06 Discovery to development of non-radio labeled molecules “cold compounds” in today’s modern life sciences research labs Dan Safarpour, U.S.A. 15:00 – 15:20 OP-A-17 Progress toward 2D microfluidic separation of DNA by length and sequence Linda B. McGown, U.S.A. 15:00 – 15:20 OP-A-21 CE-MS: an advantageous alternative to chromatography for the analysis of complex technical products Christian W. Klampfl, Austria 15:20 – 15:40 OP-A-18 15:20 – 15:40 Paper, plastic and a mobile app: modern measurement tools on the OP-A-22 cheap Vincent T. Remcho, U.S.A. Strategies for fungal characterization using capillary electrophoresis Virginia Robinson-Fuentes, Mexico 15:40 – 16:00 OP-A-19 Laser microtextured polymeric substrates functionalised with carbon nanoparticles for microfluidic-based bioanalytical applications Mercedes Vazquez, Ireland 15:40 – 16:00 OP-A-23 Capillary electrophoresis applied to the study of hemiesters of carbonic acid Claudimir L. do Lago, Brazil 16:00 – 16:20 OP-A-20 Continuous-flow Micro-electroextraction chip for enrichment of low-abundant metabolites Peter Lindenburg, The Netherlands 16:00 – 16:20 OP-A-24 The role of dried blood spots in forensic toxicology José Luiz da Costa, Brazil 16:30 – 17:30 Coffee Break, and Vendor Exposition POSTER PRESENTATION II (PP11 – PP20; PP41 – PP50; PP71 – PP80) Room Água Marinha Room Água Marinha Room Europa TECHNICAL PRESENTATIONS TECHNICAL PRESENTATIONS 17:30 – 18:00 TP-A-01 17:30 – 18:00 TP-A-02 CE/MS and LC/MS: complementary solutions for peptide mapping Celso Blatt, Agilent Technologies, Brazil An introduction to CESI: an ultra-sensitive, robust orthogonal separation tool for the characterization of a wide range of charged polar biomolecules Thomas Ed Horton, AB Sciex, U.S.A. TUESDAY, October 7: 8:00 – 18:00 Registration (Foyer) Room Água Marinha Room Europa SESSION VII – Bioanalysis & Omics SESSION VIII – Chiral & Fundamentals II Chairperson, Dr. José Luiz da Costa Chairperson, Dr. Marcone A.L. de Oliveira 8:30 – 9:00 KN-A-07 Stable-isotope-coded glycan labeling for quantitative glycan profiling: which are the specific benefits thereof? Andreas Rizzi, Austria 8:30 – 9:00 KN-A-08 Towards reliable and feasible capillary electrophoretic biomolecular interaction approaches Marja-Liisa Riekkola, Finland 9:00 – 9:20 OP-A-25 Quantitative glycomics and glycoproteomics by LC-MS/MS Yehia Mechref, U.S.A 9:00 – 9:20 OP-A-29 Comparative enantiomer affinity pattern of -blockers in aqueous and non-aqueous CE using single-component anionic cyclodextrins Jacques Crommen, Belgium 9:20 – 9:40 OP-A-26 9:20 – 9:40 The study of enzyme inhibition by various hyphenated techniques OP-A-30 Ann Van Schepdael, Belgium Enantioselective analysis of active metabolites of drugs after fungal biotransformation Anderson R.M. de Oliveira, Brazil 9:40 – 10:00 OP-A-27 Online micro-solid-phase extraction coupled with highperformance liquid chromatography for analysis of monoamine neurotransmitters and their metabolites in plasma and urine Gongke Li, China 10:00 – 10:20 OP-A-28 10:00 – 10:20 Steroid profiles as potential biomarkers in clinical diagnostics OP-A-32 measured by separation techniques and supported by chemometrics analysis Tomasz Bączek, Poland 10:20 – 11:20 9:40 – 10:00 OP-A-31 New adventures in chiral separation of helical molecules by capillary electrophoresis Dušan Koval, Czech Republic Chiral HPLC separation of selected enantiomers Taťána Gondová, Slovakia Coffee Break, and Vendor Exposition POSTER PRESENTATION III (PP21 – PP30; PP51 – PP60; PP81 – PP90) Room Água Marinha 11:20 – 11:30 Poster Tear down (all posters) Room Água Marinha Room Europa SESSION IX – Microfluidics & Microchip II SESSION X – New Applications II Chairperson, Dr. Carla B.G. Bottoli Chairperson, Dr. Anderson R.M. de Oliveira 11:30 – 12:00 KN-A-09 11:30 – 12:00 Single cell analysis of signaling KN-A-10 molecules Karel Klepárník, Czech Republic 12:00 – 12:20 OP-A-33 How low can we get? Versatile fabrication and control of microfluidic devices Carlos D. Garcia, U.S.A. 12:00 – 12:20 OP-A-37 Sustainable analytical strategies for phenolic compounds from herbal medicines María Fernanda Silva, Argentina 12:20 – 12:40 OP-A-34 Recent advances in glass microchips: addressing glass-glass bonding and improving contactless conductivity detection Emanuel Carrilho, Brazil 12:20 – 12:40 OP-A-38 Determination of biocides by capillary zone electrophoresis using UV and capacitively coupled contactless conductivity detections (C4D) Dosil P. de Jesus, Brazil 12:40 – 13:00 OP-A-35 Combining μPAD’s and microchip capillary electrophoresis for detection of explosives in soil Lucas Blanes, Australia 12:40 – 13:00 OP-A-39 Capillary electrophoresis as a guide in the isolation of active ingredients from plants and as a tool for determination of physical and chemical properties related to absorption, metabolism and excretion of the active isolates Gustavo A. Micke, Brazil Electrokinetic injection of minerals from whole fruits and vegetables Umme Kalsoom, Australia 13:00 – 13:20 OP-A-36 Use of off-stoichiometry polymer 13:00 – 13:20 OP-A-40 for microchip electrophoresis applications José Alberto Fracassi da Silva, Brazil Rapid method to the total trans fatty acids analysis in processed food by CZE-UV with direct detection Marcone A.L. de Oliveira, Brazil 13:30 – 14:30 Lunch Recess (Restaurant Potiguar – 2nd floor) 15:00 – 19:00 Symposium Tour (please meet us at the hotel lobby at 14:45 h) 20:00 – 22:00 Gala Dinner (Room Água Marinha) WEDNESDAY, October 8: 8:00 – 13:30 Registration and Withdrawal of Certificates (Foyer) Room Água Marinha SESSION XI – Young Scientists Plenary Session Chairperson, Dr. Wendell K.T. Coltro 8:30 – 8:45 YS-A-01 HPLC Determination of the structural features of flavonoids responsible for the binding to the human serum albumin Mirza Bojić, Croatia 8:45 – 9:00 YS-A-02 Application of Fӧrster resonance energy transfer (FRET) for detection of DNA mutations Vladimíra Datinská, Czech Republic 9:00 – 9:15 YS-A-03 Isoform-selective assessment of protein-protein interactions by affinity capillary electrophoresis–mass spectrometry Elena Dominguez-Vega, The Netherlands 9:15 – 9:30 YS-A-04 Dual analyser software: your assistant in optimising dual-selector (enantio)separation systems in CZE Pavel Dubský, Czech Republic 9:30 – 9:45 YS-A-05 Controlled free-radical polymerization of trimethylolpropane trimethacrylate monolithic columns for capillary liquid chromatography Mariana R. Gama, Brazil 9:45 – 10:00 YS-A-06 Electrophoretic preconcentration and separation of cationic labeled saccharides Jan Partyka, Czech Republic 10:00 – 10:15 YS-A-07 Comprehensive two-dimensional ion chromatography-capillary electrophoresis for separation of disinfection byproducts Leila Ranjbar, Australia 10:15 – 10:30 Challenges and solutions in the structure elucidation of YS-A-08 lipopolysaccharides with CE-MS and UHPLC-MS Viktor Sándor, Hungary 10:30 – 11:00 Coffee Break, and Vendor Exposition Room Água Marinha CLOSING SESSION At SERHS Natal Grand Hotel, Room Água Marinha PLENARY LECTURES II Chairperson, Dr. Emanuel Carrilho 11:00 – 11:40 PL-A-04 Studying in vivo/in vitro deamidation reactions of proteins and peptides by CE and CESI-MS Herbert H. Lindner, Austria 11:40 – 12:20 PL-A-05 Octadecyl monoliths with incorporated carbon nanotubes and neutral monoliths with coated carbon nanotubes stationary phases for HPLC and CEC of small and large molecules Ziad El Rassi, U.S.A. 12:20 – 13:00 PL-A-06 Evolution of capillary electrophoresis in clinical applications: maximizing the performance of CE technology employing a miniaturized/portable/ multi-dimensional/ multi-capillary/multi-task instrument Norberto A. Guzman, U.S.A. 13:00 – 13:30 Closing Remarks and Photography Session
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