Water PrepLC4000
94-1
PreparativeChromatography System
Product Bulletin
Routine isolation of natural,
organic synthetic, or
Waters P_epLC4000 Preparative
Chromatography Systemcombines
I.D. to 50 mm I.D. and larger.
biochemical products,
milligram to muhigram high-resolution
isolation capability with tke best price/
performance ratio of any preparative
HPIC system_Tile PrepLC 4000 System
ls ideal for routinely isolat;ng natural,
organc synthetic, cr biochemical
oroducts. Yo,_carl automate your pu"ification protocol by programming the
4000 psi backpressure capability
across the entire flow rate range
allows you to _,secolumn packings
in a wide range of partde diameters: from 3tJmon up. In addition, tubing can be easily in_erchanged to ta;Ior the fluid path for
any separation scale.
system to repetitively perform all
isolation tasks from sample loading to
purification to cdumn clean-up,
Flexibility.
• Flow rates from 0.5 ml/
rain up to 150 ml/min.
Wi,h Waters exclusive
timing chacges as flow
rate char,ges assuring
you of reproducible
mobile phase compositions across lhe entire
flow rate range for all
your separations, small
or large.
Waters
D,ws,on ol MiLl IPORF
,t
,
• Change column s;ze from 3.9 mm
• Change solvent conditions. Perform
isocra_icor multi-solvent(up to four
so:vents)gradient separations and
pick from among 11 preprogramrned gradient curves.
...................
_
¢
,
"
:
Automated
system control.
• Setup operating paramelers,edit
and crea_egradientand timed
eventmethodsfroma singlekeyboard.
•
Totalsystemintegrationthrough
Powerline'"controlof lhe pumping
system,exlernaleventsand UV
detector.
• Completemethoddocumentation
usingWaters 746 DataModule
High-ResolutionSyntheticPeptidePurification
04
0 3
Somplo_
:.,0,--_:._
_,.n'
......."_r,:_d._
Column:
Spher,cc:! ": ._ 5: 3(','0,_, 30 X 300ram
FI.......
._C'
,-_l,"r-_n
Backpressure:
Detection:
3,600 psi
.;v @ 214 rm
Gradient:
._ _()% ....... .r,.:,.q:(_,.z(.}::_ Tr/,. 90 m
02
Wok t0
Versatile fluid handling.
• Fourautomatedsolventinlets,
or Maxima 825 Chromatography
one manualsolventinlet.
• Convenient,automatedsample
injectionfor large samplevolumes.
• Flow rate rangeaccommodates
0 "'
0
t
jl
I
.......
_0
20
I
30
40
Vj__,
so
60
?0 M_outes
OD,c:,;nthe h/clhes_
resolutionposs:bleby us,ng5i;m pc_tt_c,es
pac'.<eain preparat:vecolumns
a wide range of columnsizes.
• Automatedsealwash maximizes
seal life.
Specifications
Dimensions:
Power:
29': Lx 14" W x 22" H
110 or 220V AC
Manual Loop Injector.
• Convenientmanual injectorfor
makingsingleinjectionswhen
developing methodsor checking
the purityof preparativefractions.
Temperatures:
Ooeraling: 4 ° - 40°C
Storage:0° - 50°C
4000psi (0.5 - 150 ml/rnin)
316 stainlesssteel, -[eflon, sapphire,ruby
Filled PI-I-L
Variety of Columns.
In addition to stainlesssteeland glass
columns,pre-packedPrepPak
_:''cartridgesare available in a variety of
sizesand chemistries.A PrepPak
1O00 Module isrequired to use
PrepPakcartridges.
Waters
D_ws_on of MllLIPORE
Waters ChromalographyDivision
Millipore Corporation
34 Maple
Milford,
MAStreet
01757
(508) 478-2000
Maximumpressure:
Wetted surfaces:
Sealmaterial:
Externalevents:
Outputs:
Inputs:
4 eventout (+ 12V or ground)
1 hold
1 chartout (%A,%B,%C, %D,flow)
1 pressureout
1 injector
1 stop flow
1 hold
Ordering Information.
Cat. No.
PrepLC4000 PreparativeChromatographySystem
37155
includescabinet, systemcontroller,fluid handling
unit, manual loop injector, and start-upkit.
Options:
Waters 486/IEEE TunableAbsorbance PrepUV Deteclor,
Variable 190 mm-600 mm
80642
PrepCell for usewith Waters 440/441
89599
UV Detectors
401 PreparativeRefractiveIndex Detector
SE_ 120 2-PenRecorder
48503
40307
,,.,,_:
......,,,,,_f_..:
......:_,,._.:,,,_:_,,,_.
.............
_,..,:_._
Waters 746 DataModule
_?,v,,.:_.,_c
......
-_:,o,_
f:..-'_:.:_':,oor?_'o,,_,,:,Jo,,,,,_
_
Waters FractionCollectorWith InterfaceCables
M'll'lx)f_: Coffx_:a:,or
S_- _ a r_(r s'e_l _(:.':e_r_: _ ¢: !:,'cw;
54100
37040
!:_,,,,.-:.,o
co.,,::.o,..,
,'._T:_,',v,.,',"J..'-_._.X:U_
::...... .....
s a _!_ s'.".":: "':::_u'_.':_k d F: <fi '_onl dr:
Diverter..Valve
37041
",.
......: sco
©_,oo.,..,,,,',,_:o._,
co,p.--,,_,,
......
.,,::_
........
u s.:.
N ( _ 9.'_(',,
O,,'O' (4/90)
4.:.
Preparative-Raclf,
(120 fractionsmax.)
PreparativeFunnelAdaptor
(30 fractionseach)Max. of four adap_orsper rack
PreparativeStand
37060
37046
A._s" <.1 ldloq
37050