Slovenia
Tirana, May 19th. - 21st. 2015 SLOVENE PLANT GENE BANK AND GENETIC RESOURCES PROGRAMME Lovro Sinkovič Kmetijski inštitut Slovenije Agricultural Institute of Slovenia Hacquetova 17, 1000 Ljubljana SPGR Programme Early projects to collect Slovenian autochthonous populations, ecotypes and landraces of agricultural species were initiated about 40 years ago. In 1996 the MAFF Ministry of Agriculture, Forestry and Food started financing the Slovene Plant Genetic Resources Programme with the goal to maintain, evaluate, regenerate and preserve Slovenian autochtonous species, ecotypes, populations and landraces of agricultural, medicinal and aromatic plants. They include Slovenian cultivars, old cultivars, landraces, various populations, clones and lines bred from autochtonous plants and ecotypes from the natural habitat important for food and agriculture. Organisational chart Institute of Hop Research and Brewing of Slovenia, Žalec Agricultural Institute of Slovenia, Ljubljana SLOVENE PLANT GENE BANK Central Seed Bank National Coordinator (A.I.S. LJ) Biotechnical faculty University of Ljubljana MEDPLANT Database Cooperation with NGO EKO-CI, Botanical gardens, ag. schools, … Directorat of Agriculture (MAFF) Financing and oversight over programme They have : Central Database Faculty for Agriculture and Biosistemic Sciences University of Maribor Responsability Agricultural Institute of Slovenia, Ljubljana Grain legumes, Allium, Solanum, Triticum, Brassica, Lactuca, forage and fodder crops, Rubus, Vitis, fruit trees Biotechnical faculty, Agronomy Dept., University of Ljubljana Fagopyrum, Zea mays, fruit trees, forage crops, medicinal and aromatic plants. Faculty for Agriculture, University of Maribor Prunus, Vitis, Rubus Institute for Hop Research and Brewery, Žalec Humulus, medicinal and aromatic plants Number of accessions Institution Species KIS UNI LJ BF UNI MB FKBV IHPS all all all all 3203 1635 346 143 SUM 5327 SRGB KIS Agricultural crop Inventory 1/2015 Number of accessions Species Zrnate stročnice Phaseolus spp. Vicia faba ostale Krmne rastline Trifolium sp. Medicago sp. Vicia sp. Lotus sp. Lolium sp. Festuca sp. Dactylis Phleum Travniške zeli ostale 1116 41 7 Skupaj 1164 216 43 42 35 31 102 116 45 112 242 Skupaj Poljščine Zelenjadnice Krompir Druge vrste krompirja Zea mays Triticum Secale Papaver Panicum ostale Skupaj Lactuca sp. Allium cepa Brassica oleracea Diplotaxis spp. ostale(Cichorium, Valerianella, Solanum Lyc.) Skupaj Solanum tuberosum L. Solanum sp. Solanum sp. diploidne populacije Skupaj Agricultural crop Number of accessions Species Vinska trta (kloni lastnih selekcij) Vitis Vinska trta (stare sorte) Vitis Jablana Jagoda Malus sp. Fragaria sp. Malina Robida Črni ribez Rdeči ribez Beli ribez Kosmulja Rubus sp. Rubus sp. Ribes sp. Ribes sp. Ribes sp. Ribes sp. Vaccinium corymbosum Vaccinium macrocarpon Skupaj Skupaj 984 13 6 12 26 15 63 Ameriška borovnica Brusnica 50 (na Brdu) Skupaj 135 227 31 11 18 16 303 34 18 14 66 SKUPAJ 39 (Ivanjkovci, Vrhpolje) 50 (Amp. vrt BF Kromberk pri N.G.) 89 245 (na Brdu ) 56 (na Brdu) 301 29 (na Brdu) 7 (na Brdu) 53 (na Brdu) 13 (na Brdu) 6 (na Brdu) 12 (na Brdu) 2 (na Brdu) 161 3203 Genetic resources are distributed to several collections: Grain legumes, forage crops, field crops, vegetables, vines and fruit species SRGB BF UNILJ Agricultural crop Inventory 1/2015 Number of accessions Species Navadna ajda Fagopyrum esculentum 391 Tatarska ajda Fagopyrum tataricum 15 Divja homostilna ajda Primitivna pšenica iz Črne gore Fagopyrum sp. 5 Triticum sp. Agricultural crop Krmne rastline 60 Skupaj 471 Jablana Malus domestica 119 Hruška Pyrus communis 44 Oreh Juglans regia 10 Skupaj 173 Koruza Pšenica Pšenica Oves Rž Zea mays Triticum aestivum Triticum turanicum Avena sp. Secale cereale 611 2 1 5 3 Ajda Fagopyrum esculentum 2 Ječmen Pira Tritikala Hordeum vulgare Triticum spelta × Triticosecale 1 1 1 Lan Proso Tobak Fižol Linum usitatissium Panicum milliaceum Nicotiana tabacum Phaseolus vulgaris 1 1 1 2 Buče Cucurbita sp. 4 Skupaj Zdravilne in aromatične rastline Species Arrhenatherum elatius Dactylis glomerata Holcus lanatus Lolium multiflorum Lolium perenne Phleum pratense Poa pratensis Lotus corniculatus Medicago lupulina Medicago sativa Trifolium dubium Trifolium hybridum Trifolium pratense Trifolium repens Fagopyrum esculentum Skupaj 2 13 5 13 53 1 1 16 1 15 1 1 52 23 3 200 Achillea millefolium L. Hypericum perforatum L. Origanum vulgare L. Satureja montana Gentiana lutea ostal vrste Skupaj 15 SKUPAJ 636 Number of accessions 10 10 7 3 110 155 1635 SRGB FKBV UNIMB Inventory 1/2015 Agricultural crop Species Number of accessions Koščičarji Vinska trta Jagodičevje Prunus domestica Vitis vinifera Rubus idaeus 48 288 10 SKUPAJ 346 SRGB IHPS Inventory 1/2015 Agricultural crop Species Zdravilne rastline Acorus calamus Salvia officinalis Arnica montana Valeriana officinalis Number of accessions Hmelj Humulus Lupulus 11 2 2 3 Skupaj SKUPAJ 18 125 143 Documentation of SPGB Content is divided in five fields: active Multicrop passport descriptors - (EURISCO in NI) DB maintained by MAFF next step Additional passport descriptors next step Characterization next step Evaluation active CPGB holdings – each Institution has own registry Collecting Priority: Wild relatives of cultivated crops Landraces and populations Old cultivars Collecting was organized through: Expeditions throghout Slovenia Bilateral and multilateral projects (CZ, SVK, HUN, CRO, SEEDNET, …) Donations and exchanges, Schools, adds, …. Expeditions 1999-2010 96 86 341 T 337 M Z 310 P V Diversity of collecting sites, use… Kmetijski inštitut Slovenije Hacquetova 17, 1000 Ljubljana www.kis.si Ljubljana, 2010 Some examples of collecting There was carried out informations on different: 1. Production methods Kmetijski inštitut Slovenije Hacquetova 17, 1000 Ljubljana www.kis.si Ljubljana, 2010 2. Ways of harvesting, drying, processing Kmetijski inštitut Slovenije Hacquetova 17, 1000 Ljubljana www.kis.si Ljubljana, 2010 3. Traditional dishes and home-made products Multiplication of collected samples is performed in the: field, screenhouses, greenhouses and in vitro Conservation – seed quality For conservation purposes we checked seed quality in the ISTA accredited LAB with several descriptors: - Germination - Moisture content - Seed purity Storage methods Depends on plant species – form of seeds or as a clone material (ex situ, in situ and in vitro): • • • Between 500 and 1000 seeds/accesions stored in aliminum bags or glass jams at + 4 C in working collections. Basic collection (long term storage at –20°C) is located at the Agricultural Institute of Slovenia Accessions are also kept in ‘in vitro’ conditions and ‘in vivo’ in permanent plantations for hops, fruit, grapevine and some species of medicinal and aromatic plants. Characterization and evaluation For all genetic resources we have fulfield MCPD with general informations (place, date, location,…) Descriptors EURISCO Multi Crop Passport Descriptors (MCPD) For some genetic resources we have further C&E based on Specific descriptors for individual species Characterization and evaluation examples Characterisation and evaluation: -Chemical analysis of 38 ecotypes of Dactylis glomerata L. 140 vsebnost SB (g kg -1 SS) 130 120 110 100 90 80 70 270 290 310 330 350 370 390 -Characterisation of 21 red clover ecotypes (morpf., quality (NIR)). vsebnost SVL (g kg -1 SS) -Characterisation of 17 Medicago falcata ecotypes (morpf., quality (NIR)). There were performed some Diversity studies using molecular markers 2,0 New Češnjevec 1,5 Old Češnjevec Group II 1,0 Mesoamerican outgroup Andean outgroup 0,5 New Slovenian bean Old Slovenian bean 0,0 -0,5 -1,0 Group III -1,5 -2,0 -2,5 Group I -3,0 -3,5 -2,0 -1,5 -1,0 -0,5 0,0 0,5 1,0 1,5 2,0 GR from genebank are used for Breeding programme Focused on potatoes, grasses, cloves and legumes (especially beans) The GR ar as well returned for Immediate use on farms, …. And thank you for your attention!
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