Tirana, May 19th. - 21st. 2015
SLOVENE PLANT GENE BANK AND
GENETIC RESOURCES PROGRAMME
Lovro Sinkovič
Kmetijski inštitut Slovenije
Agricultural Institute of Slovenia
Hacquetova 17, 1000 Ljubljana
SPGR Programme
 Early projects to collect Slovenian autochthonous
populations, ecotypes and landraces of agricultural
species were initiated about 40 years ago.
 In 1996 the MAFF Ministry of Agriculture, Forestry and
Food started financing the Slovene Plant Genetic
Resources Programme with the goal to maintain,
evaluate, regenerate and preserve Slovenian
autochtonous species, ecotypes, populations and
landraces of agricultural, medicinal and aromatic
plants. They include Slovenian cultivars, old cultivars,
landraces, various populations, clones and lines bred from
autochtonous plants and ecotypes from the natural
habitat important for food and agriculture.
Organisational chart
Institute of Hop Research
and Brewing of Slovenia,
Žalec
Agricultural Institute
of Slovenia, Ljubljana
SLOVENE PLANT
GENE BANK
Central Seed Bank
National Coordinator
(A.I.S. LJ)
Biotechnical faculty
University of Ljubljana
MEDPLANT
Database
Cooperation with NGO
EKO-CI, Botanical
gardens, ag. schools, …
Directorat of
Agriculture (MAFF)
Financing and oversight
over programme
They have : Central
Database
Faculty for Agriculture and
Biosistemic Sciences
University of Maribor
Responsability
Agricultural Institute of Slovenia, Ljubljana
Grain legumes, Allium, Solanum, Triticum, Brassica, Lactuca, forage and fodder crops,
Rubus, Vitis, fruit trees
Biotechnical faculty, Agronomy Dept., University of Ljubljana
Fagopyrum, Zea mays, fruit trees, forage crops, medicinal and aromatic plants.
Faculty for Agriculture, University of Maribor
Prunus, Vitis, Rubus
Institute for Hop Research and Brewery, Žalec
Humulus, medicinal and aromatic plants
Number of
accessions
Institution
Species
KIS
UNI LJ BF
UNI MB FKBV
IHPS
all
all
all
all
3203
1635
346
143
SUM
5327
SRGB
KIS
Agricultural crop
Inventory 1/2015
Number of
accessions
Species
Zrnate stročnice
Phaseolus spp.
Vicia faba
ostale
Krmne rastline
Trifolium sp.
Medicago sp.
Vicia sp.
Lotus sp.
Lolium sp.
Festuca sp.
Dactylis
Phleum
Travniške zeli
ostale
1116
41
7
Skupaj
1164
216
43
42
35
31
102
116
45
112
242
Skupaj
Poljščine
Zelenjadnice
Krompir
Druge vrste krompirja
Zea mays
Triticum
Secale
Papaver
Panicum
ostale
Skupaj
Lactuca sp.
Allium cepa
Brassica oleracea
Diplotaxis spp.
ostale(Cichorium,
Valerianella, Solanum
Lyc.)
Skupaj
Solanum tuberosum L.
Solanum sp.
Solanum sp. diploidne
populacije
Skupaj
Agricultural crop
Number of
accessions
Species
Vinska trta (kloni lastnih
selekcij)
Vitis
Vinska trta (stare sorte)
Vitis
Jablana
Jagoda
Malus sp.
Fragaria sp.
Malina
Robida
Črni ribez
Rdeči ribez
Beli ribez
Kosmulja
Rubus sp.
Rubus sp.
Ribes sp.
Ribes sp.
Ribes sp.
Ribes sp.
Vaccinium
corymbosum
Vaccinium
macrocarpon
Skupaj
Skupaj
984
13
6
12
26
15
63
Ameriška borovnica
Brusnica
50 (na Brdu)
Skupaj
135
227
31
11
18
16
303
34
18
14
66
SKUPAJ
39 (Ivanjkovci,
Vrhpolje)
50 (Amp. vrt BF Kromberk pri N.G.)
89
245 (na Brdu )
56 (na Brdu)
301
29 (na Brdu)
7 (na Brdu)
53 (na Brdu)
13 (na Brdu)
6 (na Brdu)
12 (na Brdu)
2 (na Brdu)
161
3203
Genetic resources are distributed
to several collections:
Grain legumes, forage crops,
field crops, vegetables, vines
and fruit species
SRGB
BF UNILJ
Agricultural crop
Inventory 1/2015
Number of
accessions
Species
Navadna ajda
Fagopyrum esculentum
391
Tatarska ajda
Fagopyrum tataricum
15
Divja homostilna ajda
Primitivna pšenica iz Črne
gore
Fagopyrum sp.
5
Triticum sp.
Agricultural crop
Krmne rastline
60
Skupaj
471
Jablana
Malus domestica
119
Hruška
Pyrus communis
44
Oreh
Juglans regia
10
Skupaj
173
Koruza
Pšenica
Pšenica
Oves
Rž
Zea mays
Triticum aestivum
Triticum turanicum
Avena sp.
Secale cereale
611
2
1
5
3
Ajda
Fagopyrum esculentum
2
Ječmen
Pira
Tritikala
Hordeum vulgare
Triticum spelta
× Triticosecale
1
1
1
Lan
Proso
Tobak
Fižol
Linum usitatissium
Panicum milliaceum
Nicotiana tabacum
Phaseolus vulgaris
1
1
1
2
Buče
Cucurbita sp.
4
Skupaj
Zdravilne in aromatične
rastline
Species
Arrhenatherum elatius
Dactylis glomerata
Holcus lanatus
Lolium multiflorum
Lolium perenne
Phleum pratense
Poa pratensis
Lotus corniculatus
Medicago lupulina
Medicago sativa
Trifolium dubium
Trifolium hybridum
Trifolium pratense
Trifolium repens
Fagopyrum esculentum
Skupaj
2
13
5
13
53
1
1
16
1
15
1
1
52
23
3
200
Achillea millefolium L.
Hypericum perforatum
L.
Origanum vulgare L.
Satureja montana
Gentiana lutea
ostal vrste
Skupaj
15
SKUPAJ
636
Number of
accessions
10
10
7
3
110
155
1635
SRGB
FKBV UNIMB
Inventory 1/2015
Agricultural crop
Species
Number of accessions
Koščičarji
Vinska trta
Jagodičevje
Prunus domestica
Vitis vinifera
Rubus idaeus
48
288
10
SKUPAJ
346
SRGB
IHPS
Inventory 1/2015
Agricultural crop
Species
Zdravilne rastline
Acorus calamus
Salvia officinalis
Arnica montana
Valeriana officinalis
Number of accessions
Hmelj
Humulus Lupulus
11
2
2
3
Skupaj
SKUPAJ
18
125
143
Documentation of SPGB
Content is divided in five fields:
active Multicrop passport descriptors - (EURISCO
in NI)
DB maintained by MAFF
next step Additional passport descriptors
next step Characterization
next step Evaluation
active CPGB holdings – each Institution has own
registry
Collecting
Priority:
Wild relatives of cultivated
crops
Landraces and populations
Old cultivars
Collecting was organized through: Expeditions
throghout Slovenia
Bilateral and multilateral projects (CZ, SVK, HUN,
CRO, SEEDNET, …)
Donations and exchanges, Schools, adds, ….
Expeditions 1999-2010
96
86
341
T
337
M
Z
310
P
V
Diversity of
collecting sites,
use…
Kmetijski inštitut Slovenije
Hacquetova 17, 1000 Ljubljana
www.kis.si
Ljubljana, 2010
Some examples
of collecting
There was carried out informations on different:
1. Production methods
Kmetijski inštitut Slovenije
Hacquetova 17, 1000 Ljubljana
www.kis.si
Ljubljana, 2010
2. Ways of harvesting, drying, processing
Kmetijski inštitut Slovenije
Hacquetova 17, 1000 Ljubljana
www.kis.si
Ljubljana, 2010
3. Traditional
dishes and
home-made
products
Multiplication of collected samples is
performed in the:
field, screenhouses, greenhouses and in vitro
Conservation – seed quality
For conservation purposes we checked seed quality in the ISTA
accredited LAB with several descriptors:
- Germination
- Moisture content
- Seed purity
Storage methods
Depends on plant species – form of seeds or as a clone material (ex situ, in
situ and in vitro):
•
•
•
Between 500 and 1000 seeds/accesions stored in aliminum bags or glass
jams at + 4 C in working collections.
Basic collection (long term storage at –20°C) is located at the Agricultural
Institute of Slovenia
Accessions are also kept in ‘in vitro’ conditions and ‘in vivo’ in permanent
plantations for hops, fruit, grapevine and some species of medicinal and
aromatic plants.
Characterization and evaluation
For all genetic resources we have fulfield MCPD with
general informations (place, date, location,…)
Descriptors EURISCO
Multi Crop Passport
Descriptors (MCPD)
For some genetic resources
we have further C&E based on
Specific descriptors for
individual species
Characterization and evaluation examples
Characterisation and evaluation:
-Chemical analysis of 38 ecotypes
of Dactylis glomerata L.
140
vsebnost SB (g kg
-1 SS)
130
120
110
100
90
80
70
270
290
310
330
350
370
390
-Characterisation of 21 red clover
ecotypes (morpf., quality (NIR)).
vsebnost SVL (g kg -1 SS)
-Characterisation of 17 Medicago
falcata ecotypes (morpf., quality
(NIR)).
There were performed some Diversity
studies using molecular markers
2,0
New Češnjevec
1,5
Old Češnjevec
Group II
1,0
Mesoamerican outgroup
Andean outgroup
0,5
New Slovenian bean
Old Slovenian bean
0,0
-0,5
-1,0
Group III
-1,5
-2,0
-2,5
Group I
-3,0
-3,5
-2,0
-1,5
-1,0
-0,5
0,0
0,5
1,0
1,5
2,0
GR from genebank are used for Breeding
programme
Focused on potatoes, grasses, cloves and legumes (especially beans)
The GR ar as well returned for Immediate
use on farms, ….
And thank
you for your
attention!