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INTRODUCTION OF SPECIFIC PESTICIDE‐DEGRADING BACTERIA INTO WATERWORKS SANDFILTERS ‐ A TECHNOLOGY FOR REMEDIATION OF PESTICIDE POLLUTED DRINKING WATER
Jens Aamand, Louise Feld, Lea Ellegaard‐Jensen, Christian N. Albers
The Geological Survey of Denmark and Greenland
MIRESOWA OBJECTIVE
• The overall objective of MIRESOWA is to develop new technologies for bioremediation of pesticide‐contaminated soil and water resources. • The project focuses on bioaugmentation, i.e. the introduction of specific degradative microorganisms into contaminated soil, waterworks sand filters, etc. Pesticides in Danish groundwater
Pesticides in Danish groundwater 1990-2009
50
Fund >=0,1 µg/l
fund 0,01 til 0,1 µg/l
Findings (%)
40
30
20
10
0
09
20
08
20
07
20
06
20
05
20
04
20
03
20
02
20
01
20
00
20
99
19
98
19
97
19
96
19
95
19
94
19
93
19
92
19
91
19
90
19
Introduction of pesticide‐degrading bacteria into waterworks sandfilters
Waterworks
Oxidation
Sand filter
Water tank
Consumers
Water
abstraction
well
Mn
Groundwater
Fe
Aminobacter – a BAM degrading bacterium
7.9 nM
534.1 nM
A
70%
5,270.3 nM
Mineralized to
14
CO2 (in % of added
14
C-BAM )
Aminobacter sp. strain MSH1
52,631.6 nM
60%
263,126.2 nM
50%
40%
30%
20%
10%
0%
0
1
2
3
4
5
6
7
8
9 10
Time (d)
Sørensen et al., (2007 ) Appl. Environ. Microbiol. 73:399-406
Microbial diversity of waterworks sandfilters
Gallionella dominated particularly at facility supplied
with groundwater having the highest iron concentration
Methylococcaceae were abundant in sand filters which
had significant concentrations of methane in their inlet
Hyphomicrobium spp. were abundant at all waterworks,
where they most likely perform manganese oxidation
Ammonia-oxidizing Nitrosomonas and Crenarchaeota
(Archaea) were found in the filters, albeit in relatively low
abundance.
Nitrospira, which probably plays a role in the oxidation of
nitrite to nitrate, was also present.
Albers et al., (2015 ) Environ. Sci. Technol. 49: 839–846
Poster by Lea Ellegaard-Jensen
The prokaryotic community structures of
waterworks sand filters are shaped by
groundwater chemistry
Depth (cm)
Lab‐scale waterworks
1/7-11, Before backflushing
7/7-11, After backflushing
Depth (cm)
3/8-11
Before backflushing
After backflushing
3/8-11
Pilot‐scale waterworks
BAM degradation efficiency
Loss of bacteria by backwashing
Loss of bacteria by protozoan predation
Basic filter functions reestablished rapidly Conclusions
Rapid degradation of realistic low BAM concentrations was
achieved in a pilot-scale sand filter simulating a waterworks
sand filter having a residence time of about 20 minutes.
A significant loss of degrader bacteria from the sand filters
was observed, especially during backwashing, resulting in a
decrease in BAM degradation.
Addition of degrader bacteria to waterworks sand filters did
not affect basic filter functions such as ammonium
oxidation.
Can the technology be improved?
Loss of bacteria from the sand filters may be prevented by
immobilisation of the bacteria on specific carrier materials
The treatment may be carried out in a separate reactor to
prevent loss of bacteria during backwashing
The process may be stimulated by the addition of small
amounts of organic carbon serving as a growth substrate for
Aminobacter MSH1
Partners
Danish research institutions
GEUS (Sebastian R. Sørensen & Jens Aamand)
University of Copenhagen (Flemming Ekelund, Birthe Kragelund & Søren Rosendahl)
National Environmental Reseach Institute, University of Aarhus (Ulrich Gosewinkel)
Technical University of Denmark (Arnaud Dechesne & Hans-Jørgen Albrechtsen)
Foreign research institutions
University of Leuven (Dirk Springael)
VITO (Winnie Dejonghe & Leen Bastians)
End-users
HOFOR (Ann-Katrin Pedersen)
HOFOR (Hvidovre Water), Lars Isager)
Odense Water A/S (Troels K. Bjerre)
Svendborg Water A/S (Gry Tully)
Region of Southern Denmark (Trine Korsgaard)
Agency for Spatial and Environmental Planning (Philip Grinder Pedersen)
Danish Environmental Protection Agency (Susanne Rasmussen)
Industrial partners
Krüger (Peter Borch Nielsen)
MARIBO SEED (Hans Christian Pedersen)
Partners
Danish research institutions
GEUS (Sebastian R. Sørensen & Jens Aamand)
University of Copenhagen (Flemming Ekelund, Birthe Kragelund & Søren Rosendahl)
National Environmental Reseach Institute, University of Aarhus (Ulrich Gosewinkel)
Post-doc
Arnaud
Dechesne
Technical University of
Denmark (Arnaud
Dechesne
& Hans-Jørgen Albrechtsen)
PhD Berith E. Knudsen
Foreign research institutions
PhD Lea Ellegaard
University of Leuven (Dirk Springael)
PhD Christoffer Bugge Harder
VITO (Winnie Dejonghe & Leen Bastians)
Post-doc Nadja Schultz-Jensen
End-users
PhD Zuzana Frkova
HOFOR (Ann-Katrin Pedersen)
Post-doc
Christian Albers
HOFOR (Hvidovre Water),
Lars Isager)
Odense Water A/S PhD
(TroelsAkaterina
K. Bjerre) Papadopoulou
Svendborg Water A/S (Gry Tully)
Region of Southern Denmark (Trine Korsgaard)
PhD Aswini
Sekhar
Agency for Spatial and Environmental
Planning
(Philip Grinder Pedersen)
Danish Environmental Protection Agency (Susanne Rasmussen)
Industrial partners
Krüger (Peter Borch Nielsen)
MARIBO SEED (Hans Christian Pedersen)
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