Volume 24 Issue 11 November 2014 Research Genetic and environmental exposures constrain epigenetic drift over the human life course 1725 Sonia Shah, Allan F. McRae, Riccardo E. Marioni, Sarah E. Harris, Jude Gibson, Anjali K. Henders, Paul Redmond, Simon R. Cox, Alison Pattie, Janie Corley, Lee Murphy, Nicholas G. Martin, Grant W. Montgomery, John M. Starr, Naomi R. Wray, Ian J. Deary, and Peter M. Visscher Estimating genotype error rates from high-coverage next-generation sequence data 1734 Jeffrey D. Wall, Ling Fung Tang, Brandon Zerbe, Mark N. Kvale, Pui-Yan Kwok, Catherine Schaefer, and Neil Risch Exonuclease mutations in DNA polymerase epsilon reveal replication strand specific mutation patterns and human origins of replication 1740 € ksenin, Eve Shinbrot, Erin E. Henninger, Nils Weinhold, Kyle R. Covington, A. Yasemin Go Nikolaus Schultz, Hsu Chao, HarshaVardhan Doddapaneni, Donna M. Muzny, Richard A. Gibbs, Chris Sander, Zachary F. Pursell, and David A. Wheeler Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition 1751 Scott A. Lujan, Anders R. Clausen, Alan B. Clark, Heather K. MacAlpine, David M. MacAlpine, Ewa P. Malc, Piotr A. Mieczkowski, Adam B. Burkholder, David C. Fargo, Dmitry A. Gordenin, and Thomas A. Kunkel RNA-seq of 272 gliomas revealed a novel, recurrent PTPRZ1-MET fusion transcript in secondary glioblastomas 1765 Zhao-Shi Bao, Hui-Min Chen, Ming-Yu Yang, Chuan-Bao Zhang, Kai Yu, Wan-Lu Ye, Bo-Qiang Hu, Wei Yan, Wei Zhang, Johnny Akers, Valya Ramakrishnan, Jie Li, Bob Carter, Yan-Wei Liu, Hui-Min Hu, Zheng Wang, Ming-Yang Li, Kun Yao, Xiao-Guang Qiu, Chun-Sheng Kang, Yong-Ping You, Xiao-Long Fan, Wei Sonya Song, Rui-Qiang Li, Xiao-Dong Su, Clark C. Chen, and Tao Jiang Widespread intron retention in mammals functionally tunes transcriptomes 1774 Ulrich Braunschweig, Nuno L. Barbosa-Morais, Qun Pan, Emil N. Nachman, Babak Alipanahi, Thomas Gonatopoulos-Pournatzis, Brendan Frey, Manuel Irimia, and Benjamin J. Blencowe Cell fate inclination within 2-cell and 4-cell mouse embryos revealed by single-cell RNA sequencing 1787 Fernando H. Biase, Xiaoyi Cao, and Sheng Zhong High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface 1797 OA Bianca M. Schmitt, Konrad L.M. Rudolph, Panagiota Karagianni, Nuno A. Fonseca, Robert J. White, Iannis Talianidis, Duncan T. Odom, John C. Marioni, and Claudia Kutter H3S28 phosphorylation is a hallmark of the transcriptional response to cellular stress Anna Sawicka, Dominik Hartl, Malgorzata Goiser, Oliver Pusch, Roman R. Stocsits, Ido M. Tamir, Karl Mechtler, and Christian Seiser (continued) 1808 OA Environmentally responsive genome-wide accumulation of de novo Arabidopsis thaliana mutations and epimutations 1821 OA Caifu Jiang, Aziz Mithani, Eric J. Belfield, Richard Mott, Laurence D. Hurst, and Nicholas P. Harberd Preservation of genetic and regulatory robustness in ancient gene duplicates of Saccharomyces cerevisiae 1830 Orla M. Keane, Christina Toft, Lorenzo Carretero-Paulet, Gary W. Jones, and Mario A. Fares Methods Application of histone modification-specific interaction domains as an alternative to antibodies 1842 Goran Kungulovski, Ina Kycia, Raluca Tamas, Renata Z. Jurkowska, Srikanth Kudithipudi, Chisato Henry, Richard Reinhardt, Paul Labhart, and Albert Jeltsch Unbiased analysis of potential targets of breast cancer susceptibility loci by Capture Hi-C 1854 Nicola H. Dryden, Laura R. Broome, Frank Dudbridge, Nichola Johnson, Nick Orr, Stefan Schoenfelder, Takashi Nagano, Simon Andrews, Steven Wingett, Iwanka Kozarewa, Ioannis Assiotis, Kerry Fenwick, Sarah L. Maguire, James Campbell, Rachael Natrajan, Maryou Lambros, Eleni Perrakis, Alan Ashworth, Peter Fraser, and Olivia Fletcher Linking signaling pathways to transcriptional programs in breast cancer 1869 OA Hatice U. Osmanbeyoglu, Raphael Pelossof, Jacqueline F. Bromberg, and Christina S. Leslie TITAN: inference of copy number architectures in clonal cell populations from tumor whole-genome sequence data 1881 OA Gavin Ha, Andrew Roth, Jaswinder Khattra, Julie Ho, Damian Yap, Leah M. Prentice, Nataliya Melnyk, Andrew McPherson, Ali Bashashati, Emma Laks, Justina Biele, Jiarui Ding, Alan Le, Jamie Rosner, Karey Shumansky, Marco A. Marra, C. Blake Gilks, David G. Huntsman, Jessica N. McAlpine, Samuel Aparicio, and Sohrab P. Shah Resource The landscape of human STR variation 1894OA Thomas Willems, Melissa Gymrek, Gareth Highnam, The 1000 Genomes Project Consortium, David Mittelman, and Yaniv Erlich OA Open Access paper Cover Like the opposite but interconvertible halves of Yin and Yang, the first two cells of a mouse embryo may have a tendency to choose different cell fates. In this issue, it is determined via single-cell RNA sequencing that cells in the same embryo express different genes. This suggests that mammalian embryonic cells exhibit reproducible molecular differences at much earlier developmental stages than previously thought. In the illustration, pink-colored cells represent the lineage primarily contributing to the inner cell mass and eventual offspring (the ‘‘Yang’’ half of the central embryo), whereas teal-colored cells (the ‘‘Yin’’ half) are inclined to become trophoblasts, the progenitor to the majority of extraembryonic tissues. Artwork Ó Victor O. Leshyk, 2014, www.victorleshyk.com.