Nano LPD´s
 They are extremly small vesicles (lower than
300nm)
 Mainly made of phospholipids
 The phospholipids are organised in bilayers
Nano LPD´s
Micela
Phospholipids in bilayer
Phospholipids in monolayer
Definition
Nano LPD´s
Composition
Phospholipid Structure
 They are mainly made of natural origin
phospholipids
Polar Head
((hydrophilic)
y p )
 Their characteristics are :
To have a hydrophilic part ( polar head)
 and to have a lipophilic part (apolar tail)
Apolar Tail
(lipophilic)
Nano LPD´s
Clasification
 Size:
Small (diameter < 100 nm)
 Big (diameter > 100 nm)
Nano LPD´s unilamelars
 Number of bilayers:
 Unilamelars
 Oligolamelars
 Multilamelars
Nano LPD´s multilamelars
Nano LPD´s oligolamelars
Nano LPD´s
gy between the cellular membrane structure
Analogy
and Nano LPD´s
Usages and Advantages
 They are natural delivery systems of active
ingredients
 They are controlled and released carrier
systems
Cellular Membrane
 They are structure analogues of the cellular
membranes
b
( h h li id )
(phospholipids)
 They increase the efficacy and decrease the
unwanted side effects of the active
ingredients (toxicity)
Nano LPD´s
Nano LPD´s
Damaged Skin. Low lipid content SC
Usage and Advantages
Image of the Nano LPD´s going through the SC
Skin treated with Nano LPD´s. Re-epitheliated SC
Study carried out by Dr. A De La Maza. Departament df Surfactants.
CSIC, Barcelona
Nano LPD´s
Usage and Advantages
g
Interaction Nano LPD´s – active ingredient
 The active ingredients reallocate at the
interface of the Nano-LPD´s
 Because of the structure and also the
phospholipid bilayer composition, the NanoLPD´s can incorporate: :
 Hydrophilic Actives (within the vesicle)
 Lipophilic Actives (between the layers)
Nano LPD´s
p
g
Improvement
of the analgesic
action of the
lidocaine (topically applied)
Usages and Advantages
 Prolongation of the bioavailability of
the active ingredient
Lidocaine Nano LPD´s
Lidocaine aqueous solution
Control
 B
Better
tt absortion,
b ti penetration
t ti andd
diffusion of the active ingredient
 Stabilization of the active ingredient
g
 Introduction of alternative
administration ways of the active
ingredient
Nano LPD´s Multivitamin
iti andd actions
ti
composition
VITAMINS AND THEIR DERIVATIVES IN SKIN CARE
Normalize keratinization
Downregulate sebum production in acne
Vit. A
Vit. E
VITAMIN A
Vit. C
Vit A
Vit.
Vit C
Vit.
Vit. C
Striae
Vit A
Vit. B Vit.
Vit. F
Reverse and treat photodamage
Cellulite
Antioxidant
Vit. C
Regulates collagen synthesis
VITAMIN C
Formation of stratum-corneum
stratum corneum barrier lipids
Regenerates Vitamin E
Provides photoprotection
Membrane antioxidant
Nano LPD´s Multivitamin
VITAMIN E
Protects against oxidative damage
Provides photoprotection
VITAMIN F
Cellular regeneration of the membranes and
tissues
Nano LPD´s Multivitamin
it i A
vitamin
Blocks the UV induction of the
matrix metalloproteinases (MMP)
Decreases
D
the
h collagen
ll
degradation
d
d i
Increases collagen synthesis
Inhibit UV-induced
pigmentation
Lightening of sun-induced age spots
and overall uneven pigmentation
Stimulates
fibroblast
proliferation
Plumping up the dermis
Greater thickness and resistance to
trauma
Stimulates keratinocyte
proliferation
Shedding of mature keratinocytes
Smoother skin
skin-surface
surface texture
Nano LPD´s Multivitamin
it i C
vitamin
Prevents
P
inflammation
i fl
i
Photoprotection
Prevents UV-induced
immunosuppression
Regulation
R
l ti off collagen
ll
I & III gene
transcription
Normalizes epidermal lipid profiles
(glucosphingolipids & ceramides)
Increase of collagen synthesis
Formation of stratum corneum
barrier lipids
Nano LPD´s Multivitamin
it i E
vitamin
Protective
P
i effects
ff
on UV-induced
UV i d d
oxidative damage
Photoprotection
Protection against erythema
Protection against
immunosupression and depletion of
Langerhans cells
Regulation of collagen I & III gene
transcription
Increase of collagen synthesis
Normalizes epidermal lipid profiles
(glucosphingolipids & ceramides)
Formation of stratum corneum
barrier lipids
Nano LPD´s Multivitamin
it i F
vitamin
• The polyunsatured fatty acids in vitamin F
can not be synthesised by the body
Mixture of polyunsatured fatty acids :
• Linoleic Acid C18:2
50 - 57%
• Linolenic Acid C18:3
0,7 - 1,2%
•Arachidonic Acid C20:4
0,2 - 0,4%
• Intervene in cellular regeneration of the
membranes and tissues
• Ability to modify states of the skin as
dryness, rashes and peeling
• Envigorating properties
• Improve the look of the cutis (also applied to
eliminate small folds and wrinkles)
Nano LPD´s Multivitamin
efficacy test
 In vitro test :
 Fibroblast outgrowth
 MMP inhibition
 Collagen biosynthesis
 In vivo test :
 Macro-relief of human skin
Nano LPD´s Multivitamin
fib bl t outgrowth
t
th
fibroblast
90
80
Fibroblast
Fib bl t outgrowth
t
th (% positive
iti skin
ki fragments)
f
t )
77
65
70
• Methodology :
70
65
60
55
60
50
38
40
• Freshly obtained skin samples from persons of
varying age were cut into small pieces and placed in
culture medium to allow outgrowth of fibroblasts
• Four age groups : 18-29, 30-45, 46-60, < 60 years
old
30
20
10
0
• Objective : evaluate the capability of Nano LPD´s
Multivitamin to increase fibroblast growth potential
18-29
30-45
46-60
> 60
• Data are presented as the % of skin pieces from
which fibroblast were isolated
• Results
R lt :
Untreated fibroblast
Treated fibroblast ((Nano LPD´s Multivitamin
• Fibroblast growth potential is reduced with
increased age
• Fibroblast growth potential is increased with Nano
LPD´s Multivitamin treatment
Nano LPD´s Multivitamin
MMP assays
Collagenase (MMP-1. Interstitial collagenase)
18
17
15
16
14
13
• Methodology :
12
12
• Objective : evaluate the capability of Nano LPD´s
Multivitamin to inhibit MMP concentration
10
10
• Freshly obtained skin samples from persons of
varying age
8
7
8
6
4
2
• Collagenase (MMP-1) levels were measured by
hydrolysis and quantitated with Western blot method
0
0
18-29
18
29
30-45
30
45
46-60
46
60
< 60
Gelatinase (MMP-9. 92 kDA gelatinase)
2,5
• Gelatinase levels (MMP-2 and MMP-9) were
measured by gelatin zymography and quantitated by
scanning laser densitometry
2
2
1,8
1,5
1,5
1,8
1,6
1,3
1,2
• Results
R lt :
• MMP levels are increased in skin with increasing
age
1
0,7
•MMP levels are reduced in skin with Nano LPD´s
Multivitamin treatment
0,5
0
18-29
30-45
46-60
< 60
Nano LPD´s Multivitamin
MMP assays
• Objective : evaluate the capability of Nano LPD´s
Multivitamin to inhibit MMP concentration
Gelatinase (MMP-2. 72 kDA gelatinase)
• Methodology :
2
1,8
1,8
1,6
1,4
1,4
1,2
1,1
1,4
1,2
1,1
• Collagenase (MMP-1) levels were measured by
hydrolysis and quantitated with Western blot method
0,9
1
• Freshly obtained skin samples from persons of
varying age
0,8
• Gelatinase levels (MMP-2 and MMP-9) were
measured by gelatin zymography and quantitated by
scanning laser densitometry
0,6
0,3
0,4
0,2
0
18-29
30-45
46-60
< 60
• Results
R lt :
Untreated fibroblast
• MMP levels are increased in skin with increasing
age
Treated fibroblast (Nano LPD´s Multivitamin
•MMP levels are reduced in skin with Nano LPD´s
Multivitamin treatment
Nano LPD´s Multivitamin
C ll
bi
th i
Collagen
biosynthesis
Collagen bioshyntesis (cpm x 103)
120
• Objective : evaluate the capability of Nano LPD´s
Multivitamin to increases collagen synthesis
105
100
75
80
• Methodology :
60
• Freshly obtained skin samples from aged persons
were incubated for 24h. in keratinocyte basal
medium
40
20
0
6
Untreated
Treated
Type I and III procollagen (fold increase)
5
5
4
• Type I and III procollagen (1 chain) protein levels
were assessed by western blot analysis
i
inmunohistology
hi t l
• Results :
3
2
1
• Total collagen biosynthesis was assessed by
incorporation of [14C] proline into pepsin
pepsin-resistant
resistant,
TCA-precipitatable material
15
1,5
1
1
0
Type I procollagen
Type III procollagen
• Nano
a o LPD´ss Multivitamin
u t ta
increases
c eases co
collagen
age
synthesis in aged skin
Nano LPD´s Multivitamin
M
li f off hhuman skin
ki
Macro-relief
• Objective : evaluate the capability of Nano LPD´s
u a
aat 5% too reduce
educe wrinkles
es
Multivitamin
• Methodology :
• Determination of the macro-relief of silicon replica
from 15 volunteers obteined from the area surround
the eyes.
• 4 weeks treatment, analysis of samples at time 0,
before the beginning of treatment and at 4 weeks
(28 days of application)
• The rugosity average has been assesed through
confocal profilometry with a Profilemeter Pl and
stereoscopic microscopy Optech ST3
• Results :
• Reduction of wrinkles of 25%
N=21
Ra
RMS
-25,55
-25,45
Nano LPD´s Multivitamin
 New technology :
 Nanosystems
 Natural
 < 250nm
 Controlled and sustained release
Phospholipid
 Natural active ingredients of proven efficacy
 Vitamin A
 Vitamin C
 Vitamin E
 Vitamin F
Nano LPD´s Multivitamin
 COSMETIC APPLICATIONS :
 Antiageing agent
 Antioxidant
 Prevention of photodamage
 DOSAGE :
3-5 % OF Nano LPD´s
´ Multivitamin