Genetic Diversity Underlying Differences in Minerals Content in Brassica napus 1 1 Hussein Gherli , Guy Barker & Graham Teakle 2 1- Life Sciences, Gibbet Hill Campus, Warwick University, Coventry CV4 7AL, UK 2- Warwick Crop Centre, University of Warwick, Wellesbourne CV35 9EF, UK Introduction: Oil seed rape requires addition of high levels of nitrogen fertiliser to produce the optimal yield per hectare. Not all the nitrogen applied is however transferred into the seeds. If we can increase this then we should be able to decrease the amounts required hence increasing the efficiency of production. 30 OSR varieties grown under field conditions (OREGIN field trial 2010/2011 at Rothamsted Research) were analysed. 12 mineral content has estimated following at 2 different growth stages (seed development stage GS6,2-6,3 where seeds are green and in a full size, and harvest stage) and for different plant tissues of root and stem. Different varieties behave differently under the same levels of fertiliser. This picture shows three different varieties. The differences in appearance can be striking. So can their nitrogen requirements. 0.55 0.50 GS 6,2-6,3 0.45 Harvest Nitrogen Components 1 & 2 0.40 24 26 17 0.30 14 0.25 0.15 0.10 0.05 0.00 Component 2 20 0.20 25 13 18 0.1 Component 2 N% 6 16 0.2 0.35 Spring OSR Swede Winter OSR 29 15 227 31 22 21 -0.0 3 19 1 30 28 4 8 23 5 -0.1 10 11 -0.2 9 7 -0.3 Variety 12 -0.3 -0.2 -0.1 Figure 1. Nitrogen content diversity of tap root of different varieties of Brassica napus at two different stages of growth. -0.0 0.1 0.2 0.3 0.4 Component Component11 Figure 2. Principle Component Analysis of Nitrogen content diversity of different varieties of Brassica napus. Warwick Crop Centre www.warwick.ac.uk/go/wcc
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