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Release Notes
DISCLAIMER
This software has not undergone extensive formal testing at this time. It will be included
in the next version of the Analyst® software, at which time it will have been subjected to
such testing. AB SCIEX assumes no liability or responsibility for any loss howsoever
caused in connection with using this software.
Analyst® 1.5.1HF Patch for Agilent Devices (Revision 2)
This patch adds support for the following devices in the Analyst® software:

Agilent 1290 Devices

Agilent 1260 Devices

Agilent 1367D and 1329B Autosamplers
The new features of the 1290 and 1260 devices are described in the New Features
section.
The 1367D and 1329B autosamplers are functionally equivalent to the 1367C and
1329A autosampler models, respectively.
Where to Get Help
For support, contact AB SCIEX


Email: [email protected]
Web: www.absciex.com
Prerequisites
This patch requires Analyst 1.5.1 with HotFixes to June 2010 to be installed.
Installation Instructions
This patch is compatible with Windows XP (Service Pack 3).
Note: If any version of the Analyst® 1.5.1 Patch for Agilent Devices is installed on the
computer, it will automatically be removed.
The “Analyst 1.5.1HF Patch for Agilent Devices.exe” file extracts three (3) files in the “C:\
Analyst 1.5.1HF Patch for Agilent Devices” folder by default:

Analyst 1.5.1HF Patch for Agilent Devices.msi

UpdateInfo.exe

HowToUseUpdateInfo.doc
To install the patch:
1. Deactivate the hardware profile and then close the Analyst® software.
2. Double-click the Analyst 1.5.1HF Patch for Agilent Devices.msi file and then follow
the instructions.
3. Start the Analyst software and then activate the hardware profile.
Analyst® Software Patch
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Release Notes
Updated files
The patch updates the following Analyst® software files in the C:\Program Files\Analyst\bin
folder:
Filename
Version
Filename
Version
AcqMethodDir.dll
2.5434.0.1
DDDTAgilent1100.dll
2.5434.0.1
AcqMethodSvr.dll
2.5434.0.1
DDISShimadzu.dll
2.5434.0.1
AcqMethodTimedEvents.dll
2.5434.0.1
DDPUHP1100pump.dll
2.5434.0.1
Agilent1100DTMethodEditor.dll
2.5434.0.1
EnduranceASMethodSvr.dll
2.5434.0.1
Agilent1100DTMethodSvr.dll
2.5434.0.1
ExpressViewPlate.ocx
2.5434.0.1
ASViewerCTCPAL.dll
2.5434.0.1
FamosAsMethodEditor.dll
2.5434.0.1
AutosamplerDB.adb
n/a
FamosAsMethodServer.dll
2.5434.0.1
AutosamplerDBServer.adb
n/a
HCE.dll
2.5434.0.1
AutoSamplerMethodSvr.dll
2.5434.0.1
HP1100asMethodEditor.ocx
2.5434.0.1
AutosamplerUISelect.ocx
2.5434.0.1
HP1100asMethodSvr.dll
2.5434.0.1
AutosamplerViewer.ocx
2.5434.0.1
HP1100coMethodEditor.ocx
2.5434.0.1
BatchDir.dll
2.5434.0.1
HP1100lcMethodEditor.ocx
2.5434.0.1
BatchEditor.ocx
2.5434.0.1
LCPumpMethodSvr.dll
2.5434.0.1
BatchExpress.dll
2.5434.0.1
PD__ASHP1100Sim.dll
2.5434.0.1
ColumnOvenMethodSvr.dll
2.5434.0.1
PD__COHP1100Sim.dll
2.5434.0.1
CS__VendorApp.dll
2.5434.0.1
PD__DTAgilent1100Sim.dll
2.5434.0.1
CSASHP1100as.dll
2.5434.0.1
PD__PUHP1100Sim.dll
2.5434.0.1
CSCOHP1100co.dll
2.5434.0.1
PE200lcMethodEditor.ocx
2.5434.0.1
CSDTAgilent1100.dll
2.5434.0.1
QuantOptimizeWizard.dll
2.5434.0.1
CSISShimadzu.dll
2.5434.0.1
SCL10AvpMethodEditor.ocx
2.5434.0.1
CSPUHP1100pump.dll
2.5434.0.1
StatusSvr.dll
2.5434.0.1
CTCPALV2asMethodSvr.dll
2.5434.0.1
SwitchosPumpMethodEditor.dll
2.5434.0.1
DD__VendorApp.dll
2.5434.0.1
SyncMan.dll
2.5434.0.1
DDASHP1100as.dll
2.5434.0.1
TuneMethodEditor.ocx
2.5434.0.1
DDASLCPFamos.dll
2.5434.0.1
UltimatePumpMethodEditor.dll
2.5434.0.1
DDASSHEndurance.dll
2.5434.0.1
VendorAppMethodEditor.ocx
2.5434.0.1
DDCOHP1100co.dll
2.5434.0.1
Uninstalling the patch
1. Deactivate the hardware profile.
2. Open the Control Panel and then double-click Add/Remove Programs.
3. Select Analyst® 1.5.1HF Patch for Agilent Devices from the list and then click
Remove.
Uninstalling this patch removes the ability to control these new devices.
Analyst® Software Patch
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Release Notes
New Features
The Analyst® 1.5.1 Patch for Agilent Devices adds support for Agilent’s new suite of LC
hardware products:
Agilent 1290 Hardware
 Binary Pump
Model 4220A
o Automatic purge valve for convenient system operation
 Auto Sampler
Model 4226A
o New injection volumes supported (20µl and 40µl)
 Column Oven
Model 1316C
o New 2 position / 10 port valve with RFID tag supported
 Diode Array Detector Model 4212A
o Single lamp with 190-640nm range
o User-selectable slit width up to 8nm
o Signal and Spectral data rates up to 160Hz
o RFID tag support for flow cell and lamp
Agilent 1260 Hardware
 Isocratic Pump
Model G1310B
 Quaternary Pump
Model G1311B
o Integrated low-volume 4 channel degasser
 Binary Pump
Model G1312B
 Column Oven
Model G1316A
o Maximum temperature 80°C
 Autosampler
Model G1367E
 Diode Array Detector Model G4212B
o Fixed optical slit provides 4nm bandwidth
o Signal and Spectral data rates up to 80Hz
Notes on Use
Baud Rate for Serial Communication
If the Agilent 1290 or 1260 stack is configured for Serial (RS232) communication, ensure
that the baud rate is set in the Analyst® software to 19200.
Purging and Priming using the Analyst® software
The 4220A Binary Pump now uses an integrated software-controlled purge valve.
Previously, users were responsible for manually purging and priming via a manual valve.
Analyst now supports this new functionality in both the Acquisition Method Editor and
Manual Tuning via a new Purge option.
Analyst® Software Patch
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Release Notes
New Purge Control
Figure 1: New Purge Control in Acquisition Method Editor
Ensure that the “Use” option is
selected when using the Purge option
New Purge Control
Figure 2: New Purge Control in Manual Tuning
When the Purge option is activated, the Agilent 1290 Binary Pump will open the purge
valve, allowing system purging.
When creating a purging, only the Total Time, Flow Rate and A/B(%) fields are used.
Anything entered into the Limits, Limits (Advanced) and Micro Mode tabs will be
ignored.
Users can either use Manual Tuning to purge or prime their system, or they can create
an acquisition method that will be used exclusively for purging. This method can be
submitted via the Acquisition Batch Editor in the same manner as acquisition methods.
Note that in this scenario, the mass spectrometer will acquire data to the specified .wiff
file.
Analyst® Software Patch
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Release Notes
Figure 3: Example Acquisition Batch Containing a Purge Method before Acquisition
Changes to DAD Acquisition
The Agilent 4212A and 4212B DADs have a single lamp source, rather than two lamps
as in previous DAD models. As a result, the usable wavelength range has been changed
to 190–640 nm. Additionally, the 4212A DAD supports slit widths up to 8 nm, and the
4212B has a fixed slit width of 4nm.
Analyst® Software Patch
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Release Notes
Supported Firmware
The Analyst® 1.5.1HF Patch for Agilent Devices was tested with the following firmware
versions:
Device
Firmware Revision [Build]
1290 Binary Pump G4220A
B.06.32 [005]
1290 Autosampler G4226A
A.06.32 [006]
1290 Diode Array Detector G4212A
B.06.32 [004]
1290 Thermostatted Column Oven G1316C
A.06.32 [005]
1260 Isocratic Pump G1310B
A.06.32 [010]
1260 Quaternary Pump G1311B
A.06.32 [010]
1260 Binary Pump G1312B
A.06.32 [011]
1260 Autosampler G1367E
A.06.32 [006]
1260 Diode Array Detector G4212B
B.06.32 [004]
1260 Thermostatted Column Oven G1316A
A.06.32 [005]
1200 Autosampler SL+ G1367D
A.06.32 [006]
1200 Autosampler G1329B
A.06.32 [004]
Table 1 - Tested Firmware
Note: It is possible that the patch will work with older revisions of firmware, but to
ensure maximum compatibility and interoperability, it is recommended to upgrade to
these firmware revisions when using this patch.
Analyst® Software Patch
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Release Notes
Known Issues and Limitations
Incompatibility with Hardware Profiles Created with Previous Versions of the
Agilent Devices Patch
Any Hardware Profiles created with previous versions of the Analyst® 1.5.1 Patch for
Agilent Devices or the Analyst® 1.5.1HF Patch for Agilent Devices must be recreated, as
they are incompatible with this patch. Acquisition Methods created with these patches
will continue to function as intended.
Fractional Injection Volumes are Not Supported
When fractional volumes are injected, the decimal portion will be truncated and only the
integer value will be injected. For example, if an injection of 2.5 µL is attempted, the
actual injection will be 2 µL.
DAD Acquisitions Above 40Hz Are Not Currently Supported
It is currently recommended to acquire DAD data for both the G4212A and G4212B
detectors at acquisition rates of 40Hz and below. Support for rates higher than 40Hz
has not yet been finalized.
Errors in Application Event Log When Using Older Firmware
When using firmware older than those specified in Table 1, users may see errors in the
Application Event Log when using 1260 or 1290 devices. This is caused by commands
implemented for new firmware that is unrecognized by older firmware.
Improper Labeling of Devices
If a 1260 or 1290 device is not updated to the tested firmware revision (Table 1), it is
possible that devices may be displayed as older models.
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Release Notes
This document is provided to customers who have purchased AB SCIEX equipment to use
in the operation of such AB SCIEX equipment. This document is copyright protected and any
reproduction of this document or any part of this document is strictly prohibited, except as
AB SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It
is against the law to copy, modify, or distribute the software on any medium, except as
specifically allowed in the license agreement. Furthermore, the license agreement may
prohibit the software from being disassembled, reverse engineered, or decompiled for any
purpose.
Portions of this document may make reference to other manufacturers and/or their products,
which may contain parts whose names are registered as trademarks and/or function as
trademarks of their respective owners. Any such usage is intended only to designate those
manufacturers' products as supplied by AB SCIEX for incorporation into its equipment and
does not imply any right and/or license to use or permit others to use such manufacturers'
and/or their product names as trademarks.
AB SCIEX makes no warranties or representations as to the fitness of this equipment for any
particular purpose and assumes no responsibility or contingent liability, including indirect or
consequential damages, for any use to which the purchaser may put the equipment
described herein, or for any adverse circumstances arising therefrom.
For Research Use Only. Not for use in diagnostic procedures.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective
owners.
AB SCIEX™ is being used under license.
AB SCIEX
71 Four Valley Dr., Concord, Ontario, Canada. L4K 4V8.
AB SCIEX is ISO 9001 registered.
© 2010 AB SCIEX.
Printed in Canada.
Analyst® Software Patch
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