ALGA
AE AND
D BIOFU
UELS FACILI
F
ITY
Hosted by Soutth Austrralian Re
esearch and
Develop
pment In
nstitute – Aquattic Scien
nces
This state of tthe art analytical and algal biomass produc
ction facility
y was suppported by fu
unding from
m the
Nattional Colla
aborative Research
R
In
nfrastructurre Strategy
y (NCRIS) and
a the Edducation Inn
novation Fu
unds
(EIF) under th
he Super Science
S
Initiative and h
hosted by the
t South Australian
A
Research and
Dev
velopment Institute (S
SARDI) at their
t
Aquattic Science
es division at
a West Beeach, SA. The
T service
es
pro
ovided by th
his facility are
a available to resea
archers from
m public se
ector instituutions, universities and
d
industry on a fee for servvice basis.
ANA
ALYTIC
CAL CA
APABILITY
PROTEIN
P
A
ANALYSIS
S
Extraction
E
n
•
•
•
•
Chemiical extracttion of Tota
al Proteinss using Triz
zol
Preferrred form of samples - homogen
neous dried powder
Minimu
um sample
e size = 0.5
5g
Minimu
um numbe
er of samplles = 10
Quantifica
Q
ation
• Quantiification of Total proteein contennt
• Coomaassie Braddford spectrophotom etric assayy
• Minimuum numbeer of samplles = 10
Character
C
risation
• Agileent 2100 bbioanalyser
• Sizinng and quaantitation of
o proteins based on on-chip ge
el
elec
ctrophoresiis
• Miniimum num
mber of sam
mples = 10
FATTY
F
AC
CID PROFIILES
Quantifica
Q
ation by Gas Chrom
matograph
hy – Flame
e Ionizatio
on Detecto
or (GC-FID
D) and
Identificati
I
ion by Ga
as Chroma
atographyy – Mass Spectrome
S
etry (GC-M
MS)
• Perkkin Elmer G
GC-MS (Clarus 560s
s) for Fatty acid methylated este
er
•
•
•
•
•
(FAM
ME) identiffication
Perk
kin Elmer G
GC-FID (C
Clarus 500) for quantiffication
Exte
ernal stand
dards conta
aining 30 FAMEs
F
useed for qualitative
anallysis
Prefferred form
m of sample
es - homog
geneous drried powde
er
Miniimum samp
ple size = 0.5g
Miniimum num
mber of sam
mples = 1
TOTAL
T
LIP
PID CONT
TENT
Hot
H Soxhllet extractiion and qu
uantificatiion
• Gerhardt Soxttherm, autoomatic hot soxhlet syystem for tootal lipid
dete
ermination in solid sa
amples
• Prefferred form
m of sample
es - homog
geneous drried powde
er
• Miniimum sampple size = >5g
>
• Miniimum num
mber of sam
mples = 6
Gravimetr
G
ric low tem
mperature extraction
n and qua
antification
n
• Buchi Rotavappor R210, Buchi V7
700 and V
V850, prov
vides a miild
apprroach to tthe determ
mination of
o total lippids in so
olid sample
es
without excesssive heatin
ng.
• Prefferred form
m of sample
es - homog
geneous drried powde
er
• Miniimum sampple size = 5g
• Miniimum num
mber of sam
mples = 1
Modified
M
B
Bligh & Dyyer extraction and g
gravimetric quantific
cation
•
•
•
•
Modifie
ed Bligh & Dyer technique for to
otal lipid estimation
Ideal fo
or sample size < 5g and
a or whe
ere lipid co
ontent is prredicted to be < 5%
Estima
ates crude total lipid content
c
Minimu
um numbe
er of samplles = 6
LIPID
L
CLA
ASSES
Qualitative
Q
e and qua
antitative analysis
a
o
of lipid clas
sses
•
•
•
•
•
Includess Triglycerides, Phos
spholipids a
and Free Fatty
F
Acids
s
Qualitattive analysis using GC
Quantita
ative analyysis using Single
S
Pha
ase Extracttion (SPE) columns
Preferre
ed form of samples
s
– extracted samples or
o homogeneous drieed powder
Minimum
m number of samples = 4
TOTAL
T
CA
ARBOHYD
DRATES
Total
T
carbohydrate quantificat
q
tion
•
•
•
•
•
Pheno
ol-sulfuric acid
a
spectro
ophotomettric assay for
f Total ca
arbohydrattes
Calibra
ated againsst standard
d glucose
Preferrred form off samples - homogen
neous dried powder
Minimu
um sample
e size = 0.5
5g
Minimu
um numbe
er of samplles = 6
GLYCOGE
G
EN
Glycogen
G
quantifica
ation
•
•
•
•
•
Spectrrophotome
etric quantiffication usiing Anthrone techniq
que
Calibra
ated against standard
d glycogen
n
Preferrred form of samples - homogen
neous dried powder
Minimu
um sample
e size = 1g
g
Minimu
um numbe
er of samplles = 12
Page 2 of 8
CALORIFI
C
C VALUE (ENERGY
Y ESTIMAT
TION)
Calorimete
C
er Energyy
• IKA Oxygen boomb caloriimeter calibrated agaainst Benzooic acid
•
•
•
•
stan
ndards
Dete
ermines th e gross ca
alorific valu
ue for liquidd and solid samples
Prefferred form
m of sample
es - homog
geneous drried powde
er
Miniimum samp
mple size = 0.6g
Miniimum num
mber of sam
mples = 6
TOTAL
T
CA
ARBON AN
ND NITRO
OGEN ANA
ALYSIS
Total
T
orga
anic carbo
on, total inorganic ca
arbon, tottal carbon, total bou
und nitrogen
• Analytic Jennaa Multi N/C
C analyser
• Analysis of TC
C, TOC and
d TIC in liq
quid and soolid sample
es and the
TNb in liquid sa
amples
• Miniimum sampple size = 1g
1 carbon only
o / 10mll liquids
• Miniimum numbber of sam
mples = 6
Total
T
Kjeld
dahl Nitrog
gen and Protein
P
•
•
•
•
•
Tota
al Kjeldhal Nitrogen in solid and
d liquid sam
mples
Buchi Total Kje
eldahl Nitrrogen Analyser
Crud
de protein (calculated
d) from TK
KN values
Miniimum samp
mple size = 1g
Miniimum numb
ber of sam
mples = 4
ELEMENT
E
TAL ANALY
YSIS
Inductively
I
y Coupled
d Plasma – Optical Emission Spectrom
metry (ICP
P-OES)
•
•
•
•
•
ICP--OES Varia
an Vista-P
PRO
Trac
ce and maccro-elemen
ntal analys
sis in liquidd and solid samples
Solid
d samples will requirre digestion
n.
Miniimum samp
ple size = 7ml of liqu
uid / 1g of ffine powde
er
Miniimum num
mber of sam
mples = 6
INORGANIC DISSOL
LVED NUT
TRIENT AN
NALYSIS
Ammonium
A
m, Nitrate
e, Nitrite, Total
T
Oxid
dised Nitro
ogen, Urea
a, Phosph
hate, Silica
ate
• Aquakem 200ccd discretee nutrient analyser
a
• Deteermination of the nutrient conce
entrations in clean water (fresh
h,
•
•
•
•
sea or brackis h), wastew
water, proc
cess water,, effluents and growth
h
med
dia.
High
h throughp
put ~60 sam
mples per analate
a
peer hour
Inclu
udes Nitratte, Nitrite, Total Oxidised Nitroggen, Urea, Phosphatte,
Amm
monia, Silicca
Miniimum samp
mple size = 50ml
Miniimum numb
ber of sam
mples = 10
Page 3 of 8
RADIOISO
R
OTOPE QU
UANTIFICA
ATION
14
1
C, 3H, 32P and 33P analysis
• Perkkin Elmer TTri Carb 29900tr liquid
d scintillatioon counterr
• Deteection and counting of
o alpha, beta and gaamma radioactivity
• Proccessed sam
mples in liq
quid scintillation cockktail in scin
ntillation via
als
TITRIMETR
T
RIC ANAL
LYSIS
Total
T
alkalinity
•
•
•
•
•
Me
etrohm Ele ctrotitratorr (719 Titrin
no)
Endpoint titra
ation with a strong ac
cid
Total alkalinitty correspo
onds to titrable alkali nity as pH 4.5
Min
nimum sam
mple size = 100ml filtered
Min
nimum num
mber of sa
amples = 10
0
Acid
A
value
e and free
e fatty acid
d percenta
age (%FFA
A) of biodiesel, fatss and oils
•
•
•
•
Qu
uantification
n of acidity
y and %FFA of biodieesel, fats and
a oils
Co
olourimetricc titration with
w a stron
ng base
Ep
ppindorf Mu
ultipipette X-Stream
X
Min
nimum sam
mple size = 30g (acid value annd %FFA), 3g (%FFA
A
only)
• Minnimum num
mber of sa
amples = 1
Karl
K Fishe
er moisture
e determin
nation
• 831 KF Couloometer, for the Karl Fisher
F
deteermination of moisturre
con
ntent in bio
odiesel, fatts and oils
• Minnimum sam
mple size = 10g
• Minnimum num
mber of sa
amples = 5
MOISTURE
M
E / ASH / TOTAL
T
SU
USPENDE D SOLIDS
S / PARTIC
CULATE O
ORGANIC MATTER /
PARTICUL
P
LATE INOR
RGANIC MATTER
M
A
ANALYSIS
S
Moisture
M
A
Analyser
• Metttler Toledoo HG63 mooisture ana
alyser
• Rappid and preecise methood to deterrmine moissture conteent in solid
sam
mples
• Miniimum samp
mple size = 1g
• Miniimum num
mber of sam
mples = 1
Gravimetr
G
ric determination of ash
•
•
•
•
•
Gra
avimetric d
determination of mois
sture/ash ccontent
Co
ombustion in a muffle
e furnace at 500-550°°C
Further analyysis for acid soluble and
a acid innsoluble an
nalysis
Min
nimum sam
mple size = 1g
Min
nimum num
mber of sa
amples = 10
0
Page 4 of 8
ROTENON
R
NE ANALY
YSIS
High
H
Perfo
ormance Liquid
L
Chrromatogra
aphy
• Shim
madzu HP
PLC system
m equipped with Ph otodiode Array
A
(PDA
A)
•
•
•
•
and refrigerate
ed autosam
mpler
Qua
antification of rotenon
ne in solid and
a liquid samples
Calibrated aga
ainst roteno
one standa
ard
Miniimum samp
mple size = 1g
Miniimum num
mber of sam
mples = 1
ADD
DITION
NAL EQ
QUIPME
ENTS
• Flow cyytometer (B
Beckman-c
coulter Ce ll lab Quan
nta SC)
• 18L Freeeze Dryer (Labconcco)
• Milli Q Integral Water
W
Purific
cation Sysstem (TOC <5ppb) an
nd a Millipoore Elix 5 Reverse
R
Osmossis system
•
•
•
•
•
86L -80
0ºC Freeze
er
Refrige
erated centtrifuge (Eppendorf 58
810 R, max
ximum cap
pacity 4 x 2250ml)
Muffle furnace (1100ºC)
2 x fum
mehoods
Varietyy of precisio
on balance
es from 0.0
01mg – 4 kg
k resolutio
on
PHOTOSY
P
YNTHETIC PIGMENT
TS
High
H
Perfo
ormance Liquid
L
Chrromatogra
aphy – Pig
gment pro
ofile suite
• Shim
madzu HPL
LC system
m equipped with Fluorrescence detector
d
an
nd
refrigerated au
utosamplerr
• Photosyntheticc pigment profiles fro
om solid orr liquid sam
mples
• Calibrated usinng 19 phottosyntheticc pigment sstandards
• Miniimum samp
mple size = 0.2g
Spectroph
S
hotometer/
r/ Spectroffluoromete
er / Microp
plate Rea
ader - Qua
antification of pigments
• Synergy 4 Miccroplate reaader, Shim
madzu UV-11700
•
•
•
•
Spectrophotom
meter and Hitachi F2500 Specttroflurometter
Qua
antification of Chlorop
phyll a, b, c and total carotenoid
ds
Acetone / Etha
anol extrac
cted samples
Miniimum samp
mple size = 500mg of dried powdder or sam
mple supplie
ed
on 0.2
0 µm pore
e size cellulose aceta
ate or glasss fiber filterr paper
Miniimum num
mber of sam
mples = 3
Page 5 of 8
PHOTOPH
P
HYSIOLOG
GICAL ASS
SAYS
Walz
W
Phytto-PAM an
nalyser
• Phyto-PAM m
measures emitted
e
fluorescencee at 4 excitation
wa
avelengths
• Assessment of photosyynthetic performancee and light adaptationnal
sta
ate of phyto
oplankton
• Samples mayy be microoalgal cultures or natuural phytopplankton
sam
mples
Walz
W
Divin
ng PAM analyser
a
• Unnderwater ffluorometeer
• In situ
s measu
urements of
o chloroph
hyll fluoresccence
• For use on seeagrassess, macroalg
gae, coral zzooxanthelle
Aquation
A
S
Submersible Photophysiolog
gical Syste
em
• Divver deployeed submerrsible cham
mber systeem with shu
utter
•
•
•
•
•
fluo
orometers and senso
ors for PAR
R, temperaature, pH and
a salinityy
Da
ataloggers and powerr modules allow for uunattended
d operation
ns
Pumps for m ixing and flushing
f
In situ
s measu
urements photosynth
p
hesis
For use on se
eagrasses
s and macrroalgae
Set of 10 cha
amber systtem includiing 2 blankk chambers
WATER
W
QUALITY ANALYSIS
A
pH,
p
conductivity, temperatu
t
ure, carbo
on dioxid
de (CO2), dissolveed oxygen
n (DO), light,
density,
d
viiscosity
nt of param
meters inclu
uding:
• Meeters availaable for meeasuremen

pH, conductivity, temperature, CO2 , DO, lightt, density,
visco
osity
Page 6 of 8
ALGAL PRODUCTION SY
YSTEMS
S
SMALL
S
LA
ABORATO
ORY SCAL
LE EXPERIIMENTAL SYSTEM
•
12 x Te
eco Aquarrium heater/chillers (8
800L-2000
0L capacity
y)
•
Aquatrronica conttrol and mo
onitoring ssystem with
h pH and te
emperaturee sensors
•
24 x 20
00W LED lights – Pla
ant lights ccapable of yielding up
p to 1500μ
μmol.m-2.s-1 PAR
MASS
M
CUL
LTURE INFRASTRU
UCTURE
Pure
P
culture and iso
olation faciility
Inclu
udes:
• Laminar Fllow Cabine
et
• Petriplate ccolony Cou
unter
• Fluorescen
nt microsco
ope (Olympus BX-611) and Digital inverted
d
microscop e (EVOS) for digital imaging, qquantificatio
on of
intracellula
ar lipid prod
duction and automateed cell cou
unts
• 110L Autocclave (Hira
ayama HV)
Controlled
C
d Environm
ment Room
m
• Co
ontrolled en
nvironment room with temperatuure and ligh
ht control and
CO
O2 input
• 2 x light and ttemperaturre controlle
ed growth ccabinets
Micro
M
race
eway syste
em (250L, 2.5m2)
2
• 2 x 2.5m
2
race
eway ponds
s including control sysstems for CO
C 2 injectio
on
and pH contro
ol
R&D
R
scale
e racewayy system (3
3500L, 20
0m2)
• 9 x 20m
2 2 racew
way ponds including automated
a
control and
mon
nitoring sysstem for CO
O2 injection, pH, waterr level, salinity and DO
O
mon
nitoring for cultivation of micro- and
a macroaalgae
Bench
B
top
p bioreacto
or (12L)
• 12L bench top Applicon fermentor
f
in
ncludes coontrol syste
ems for
temp
perature, p
pH, DO, CO
O2 injection, nutrient feeed, data lo
ogging and
d
mixing. Bolt on
n lights ava
ailable for use as a phhotobioreac
ctor. Ideal fo
or
stud
dies on auto
otrophic, mixotrophic
m
and heterootrophic systems.
Large
L
scalle - closed
d system (3500L)
• 3 x Pilot-Scale photobio
oreactor comprising off a 3.5m3 Algaelink
A
sollutions tubu
ular reactor, with auto
omated reggular injection of
nuttrients and CO2
Page 7 of 8
DOWN
D
STREAM PR
ROCESSIN
NG EQUIPM
MENTS
• Bio
o-ULTRA iss a pilot sca
ale automa
ated biodiessel reactorr that uses
ultrrasound to enhance the
t transes
sterificationn of methan
nol and oil to
t
form high qua
ality biodies
sel under controlled
c
teemperature
e.
• 2 x Clara 80 IIndustrial process
p
cen
ntrifuges
• Us
sed for harvvesting alga
al biomass
s from masss culture sy
ystems
• Associated in
nfrastructurre includes, seawaterr intake, filtrration and
dis
sinfection, ssedimentattion tanks and
a storagee for algal biomass
b
• My
yers vacuum
m centrifug
gal molecullar distillatioon system for
sep
paration an
nd purificattion feed materials succh as oils
• Ca
an be applie
ed for puriffication of Polyunsatu
P
rated Fatty
y Acids from
m
alg
gal lipids
• Als
so used in cchemical, food
f
proces
ssing, pharrmaceutica
al and
pettroleum pro
ocessing
Con
ntacts:
Drr Sasi Nay
yar
Fa
acility Mana
ager
Te
el: +61 8 82
207 5321
Em
mail: sasi.n
[email protected]
gov.au
Mrrs. Michellee Braley
Laboratory M
Manager
Tel: +61 8 82207 5330
E mail:
m micheelle.braley@
@sa.gov.au
u
Page 8 of 8
ALG
GAE AND BIOFUELS FAC
CILITY
SARDI Aquatic Scie
ences
2 Hamra A
Avenue West Beach SA
A 5024
P: (08) 88207 5321 F:
F (08) 8207
7 5481