Master studieretning mikrobiologi
Bakterie
r
Virus
Evolusjon
Forskningsgrupper:
1. Marin Mikrobiologi
2. Generell Mikrobiologi
3. Senter for Geobiologi
Mikrobiologi
Alger
Økologi
Ekstremofile
Modellerin
g
.
1. Marin Mikrobiologi
Forstå hvordan den mikrobielle sløyfen i det marine næringsnettet fungerer
-Hvem er de (Virus, Prokaryote, encellede alger (protister)) ?
-Hva gjør de?
-Hvordan er sammspillet mellom dem?
-Hvordan responderer de mht miljøforandringer?
MICROPOLAR
THE ARCTIC FOOD CHAIN
..and the central role of the microbes
Primary production to harvestable
resources
Export to the
ocean’s interior
Nutrient (DIN, DIP),
carbon (CO2, DOC)
chemistry
Activities
• Field campaigns
– “Annual cycle” (pelagic) NW
Svalbard, different water masses
(2015)
– SIMCO north of Spitzbergen
(2016) (+ samples from N-ICE
2015, NPI)
– Young Sound, Greenland (2015)
• Field experiments
– Mesocosms – June 2015
– Grazing exps (during field
campaigns and YS)
• Workshops and data analyses
Structure, function and diversity
protist-, prokaryotic-, viral
communities
(molecular)
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Protists:
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Prokaryotes:
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Filter various size classes - for diversity & taxonomic info - pyrosequencing rRNA gene tags (SSU & LSU rDNA)
Metagenomic data from fcm-sorted populations
mRNA  to compare gene expression of enzymes involved in the production and consumption of DOC and
CO2
Filter and extract DNA (history) and RNA (snapshop active community) simultaneously from same sample
Sequencing DNA of a 287 bp-long region within 16S rDNA (V4-5; 519F- 806R) (Illumina MiSeq)
Virus:
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Concentrate viral fraction - Illumina MiSeq sequencing
Tag sequencing: ssRNA virus (dino- and diatom-virus?),
MCP (phytovirus), PhoH (phages)
Metagenomic sequencing (some samples only)
Mesokosmeforsøk
• Stimulerer virkelige
miljøer, men hvor
miljøfaktorer kan
manipuleres
Eksempler på nylig gjenomførte
masteroppgaver
Diversity of Artic marine viruses in a depth
profile
(Emily Olesin, pågående)
Metagenomics
Phylogeny and functional studies of Thalassiosira sp. isolated from
Kongsfjorden, Svalbard (Trine Strand Jensen, 2012)
Coccolithophorides around Svalbard identified from Scanning Electron
Microscope (SEM)(Sesilie Færøvik Tverberg, pågående)
Marine microbial community in arctic summer north of Svalbard
By RNA and DNA community analyses
Enrichment of Bacteria from Lake Sælenvannet
Eva Lena Nordmann, Erasmus student (2014)
Characterization of microbial communities in moderate to severe acid
mine drainage sites associated to arctic coal mining in Svalbard
(Andreas Austnes, 2012)
Theme
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Supervisor
Email
Temperature effects on viruses and bacterial production
(Arctic relevance).
Biological calcite production in the ocean (Climate
relevance).
Prof. Gunnar Bratbak
[email protected]
Phytoplankton
Prof. Jorun Egge
[email protected]
1.aman Svein Rune Erga
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Light and vertical migration of microalgae
[email protected]
[email protected]
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Viruses and interaction with their hosts (Prokaryotes and
Eukaryotes)
Prof. Ruth-Anne Sandaa
Prof. Frede Thingstad
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Ecological theory, nutrient cycles
[email protected]
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Microbial life in meromictic lake Sælenvannet
Characterisation of Permafrost in arctic soils
Prokaryotic diversity in Polar water and sea Ice
Climate change and the effect of melting sea ice and
thawing permafrost on its indigenous microorganisms.
Prof. Lise Øvreås
[email protected]
Researcher Aud Larse
(UniEnvironment/BIO)
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Protist interactions (Arctic relevance)
[email protected]
Nye masteroppgaver i Marin Mikrobiologi:
http://www.uib.no/en/rg/mm/55646/masterprojects
2. Generell mikobiologi
(http://www.uib.no/en/rg/gm)
Arbeidsområder
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Mikrobiell diversitet i ekstreme miljø
Molekylær mikrobiologi
Termofile Archaea og anaerobe bakterier
Metanoksyderende bakterier
Varmeadapterte enzymer og bioteknologi
Geomikrobiologi og oljerelaterte bakterier
Syntetisk biologi
Enteropatogene bakterier
Group leader:
Nils-Kåre Birkeland
Avdelingsingeniør
Birte Töpper
Treponema thermophilum - en nyisolert termofil spirochaet
Eksempler på nylig gjennomførte masteroppgaver:
Analyses of Thermotogales secretomes (Trude With, høst 2014)
The Hfq RNA chaperone in the deep-branching Thermotogales lineage:
Attempts to reveal its biological role (Oda Barth Vedøy, høst 2014)
Phylogeny and diversity of new isolates of aerobic thermoacidophilic
methane oxidizing Verrucomicrobia (Natalie Bennett Smestad, høst 2014)
Cultivation and genomics of extremophiles from a deep and hot North Sea oil
reservoir (Anne Marie Lerang, høst 2014)
Prokaryotic diversity in an Armenian geothermal spring using metagenomics,
anaerobic cultivation and genome sequencing (Lianna Poghosyan, vår 2015)
Acidobacteria
Bacteroidetes
Chloroflexi
Elusimicrobia
Firmicutes
Fusobacteria
Ignavibacteriae
Planctomycetes
Proteobacteria
Spirochaetes
Synergistetes
Verrucomicrobia
Uncultured Candidate division WS6
Actinobacteria
Her kan dere finne forslag til Masteroppgaver
i generell mikrobiologi:
http://www.uib.no/en/rg/gm/56030/msc-thesis-projects
3. Senter for Geobiologi
Senter for geobiologi er (CGB) samarbeid
mellom Institutt for geovitenskap og Institutt for
biologi.
• ny kunnskap omkring vulkanske og
hydrotermale prosesser i dyphavet,
• liv i ekstreme miljøer,
• jordens tidlige utvikling og livets opprinnelse.
Emner –Studieretning Mikrobiologi
Obligatoriske kurs
Høst
• BIO300: Biological Data Analysis and Research Design (Master
course)
• BIO217: Microbial ecology (Focus on Marine microbial
ecology, lab experiment with mesocosm)
• BIO 215: Microbiology: General Microbiology
Spring
• BIO 218: Experimental Microbiology (genetics and physiology)
Valgfrie kurs:
Avhengig av valg av oppgave og kan diskuteres med potensielle veiledere
Eks:
• BIO315: Selected Topics within Microbiology (5 st - irregular) Content may change
depending on students and teachers: Discussion of important papers in microbial
ecology. Exam : presenting the papers with discussion
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Molecular biology
Bioinformatics
Marine diversity (algal diversity)
Virology
Geobiology
Geophysics
Mathematics
Marin økologi