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StarPeptides
The next generation
of biomaterials
A. Sulistio et al., Biomacromolecules,
2011, 12, 3469-3477.
N. Wiradharma et al., Small,
2012, 8, 362-366.
Magainin I Star Peptide
N. Wiradharma et al., Small, 2012, 8, 362-366.
USP Star
BioBrick Expression System
SUMO tag: B. Bommarius et al., Peptides, 2010, 31, 1957-1965.
Expression
Lysis & Purification
Western Blot
Coomassie Stain
22.15 kDa
29.3 kDa
14.32 kDa
Coomassie Stain
Coomassie Stain
Summary of Findings
• Positive expression results
• SUMO sequence found
• Further characterization
• Key step to star peptides
Children’s
book
Book readings
at childcare
centres
National
Science Week
Underrepresented
students
Supporting
university iGEM
team
Well resourced
private high
school
Melbourne 2014
Acknowledgements
Associate Professor Heung-Chin Cheng
Pat Shilling and the Gooley Lab
Bharath Kumar from the 2013 TU Delft iGEM team
Allowed us to use his lab space and arranged for training on
practical procedures, helping with troubleshooting and theory
Were a great help in designing experiments and protocols
Provided valuable advice about the Delft SUMO expression
system.
George Grigorian
Associate Professor Paul Gooley, Dr Angus Johnston, and
Associate Professor Neil O’ Brien-Simpson
2014 TU Delft iGEM team
Designed the layout of the website
Supplied us with their UlpI gene.
Provided advice on our gene construct and helped us with
brainstorming
Luke Thorburn and Nic Roumeliotis
Bianna Makogon
Did the amazing videos visible at our website
Daisy Cheng
For work on the book "The Adventures of E. Coli"
Members of the Bio21 Proteomics Facility
Assisted the team with laboratory techniques and reviewed our
protocols
Students of the Cheng Lab, including
Ashfaqul Hoque, Sze-ting Bong, Gahana Advani, Ken Ang, George
Cao, David Zula, Anna, and Ya Chee
Provided incredible amount of help in the lab
Melissa Warner
Ran our samples through mass spectrophotometers and
helped us to interpret the data
Staff of Bio21 administration, including Peter Coles and
Helen Varnavas
Were extremely helpful in helping us order supplies and
administering the team
For the design of the Melbourne iGEM logo
Sponsors
Founding sponsors of the iGEM team
Official suppliers and sponsors of the iGEM team
Live Long and
Prosper