UC Davis Championship Poster
OliView: An Enzyme-Mediated Electrochemical Biosensor for Olive Oil Quality Control E. Aaron Cohen, James Lucas, Lucas Murray, Sarah Ritz, Yeonju Song, Simon Staley, Brian Tamsut Dr. Marc Facciotti, Dr. Justin Siegel, Dr. Ilias Tagkopoulos, Dr. Selina Wang University of California, Davis: UC Davis Genome Center; UC Davis Olive Center; College of Agricultural and Environmental Sciences; College of Biological Sciences; College of Engineering Introduction Methodology: Project Overview Signal Processing 70% of βExtra Virginβ Olive Oil Consumed in the United States is Rancid from Exposure to Heat and Light We trained a predictive model on a combinatorial library of aldehydes based on observed enzyme steady-state velocities Policy and Practices Enzyme Engineering Electrode Potentiostat Signal Processing Aldehyde Dehydrogenases with Unique Substrate Specificities Policy and Practice California Department of Food and Agriculture ππππ = πππ β πππ + πππΏ β πππΏ + ππ β ππ Average Catalytic Efficiency Medium Saturated Aldehydes (C5-C7) WT E. coli ALDH BBa_K1324000 W176Q E. coli ALDH California Olive Oil Producers Community Olive Oil Tasting BBa_K1324001 WT Rat ALDH BBa_K1324003 100% Long Saturated Aldehydes (C8-C10) 95% Unsaturated Aldehydes 8% 98% 100% 2% 100% 75% 71% Parts Submitted BBa_K1324000: Wild-Type Escherichia coli Aldehyde Dehydrogenase BBa_K1324001: W176Q Escherichia coli Aldehyde Dehydrogenase BBa_K1324002: W176V Escherichia coli Aldehyde Dehydrogenase BBa_K1324003: Wild-Type Rattus norvegicus (Rat) Aldehyde Dehydrogenase Indicators of Rancidity: Aldehyde Composition in Olive Oil is Complex Medium Saturated ππ ππΏ π Dropsens Electrode Detects Enzyme-Generated NADH ππΈ1π π = ππΈ2ππ ππΈ3π π ππΈ1π ππΈ2π ππΈ3π πΏ πΏ πΏ ππΈ1π ππΈ2π ππΈ3π β1 ππππ πΈ1 β ππππ πΈ2 ππππ πΈ3 Our Mathematical Model Successfully Identified Two out of Three Rancid Olive Oils in a Blind Test Our Open Source Software Package Contains: β’ Measurement packages for anodic stripping, cyclic voltammetry, and potentiostatic amperometry β’ Signal Conditioning and Processing β’ Statistics Package β’ Enzyme Specificity Determination Tool Performance Comparison Against an Industry Standard Potentiostat Acknowledgements Long Saturated Unsaturated $2,000-$10,000 $60 Academic Sponsorshipβfor their generosity and enthusiasm β’ Vice Chancellor of Research, Harris Lewin, Ph.D. β’ College of Biological Sciences - Dean James Hildreth, Ph.D. β’ College of Agriculture - Dean Helene Dillard, Ph.D. β’ College of Engineering - Dean Enrique Lavernia, Ph.D. β’ UC Davis Genome Center - Director Richard Michelmore Ph.D. β’ Department of Biomedical Engineering - Chair Kyriacos Athanasiou, Ph.D., P.E. Corporate Sponsorsβfor their technical support and generosity β’ Pine Instruments β’ Corto Olive Company β’ Dropsens β’ California Olive Ranch β’ Life Technologies β’ Seka Hills Olive Mill β’ BASF β’ Sigma Aldrich β’ Transcriptic
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