8.1-8.3 WORKSHEET Section 8.1 – Identifying DNA as the Genetic Material KEY CONCEPT - DNA was identified as the genetic material through a series of experiments MAIN IDEA: Griffith finds a “transforming principle.” Write the results of Griffith’s experiments in the boxes below Experiments 1. Injected healthy mice with R type bacteria Results (what happened to the mice?) 2. Injected healthy mice with S type bacteria 3. Heat-killed the S type bacteria and then injected them into healthy mice 4. Mixed the S heat-killed with R type bacteria and then injected both of them into healthy mice. The mice died. Found live S type bacteria in the healthy mice 5. Which type of bacteria caused the deadly disease, the S type or the R type? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 6. What was “transformed” in Griffiths experiment? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 7. What conclusions did Griffith make based on his experimental results? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 8. What were the three tests that Dr. Avery did that added to Dr. Griffith’s experiment? a. ______________________________________________________________________________________ ______________________________________________________________________________________ b. ______________________________________________________________________________________ ______________________________________________________________________________________ c. ______________________________________________________________________________________ ______________________________________________________________________________________ 9. What was the conclusion? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 10. Describe what Dr. Hershey and Dr. Chase did, what was their experiment about? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 11. What was the conclusion? __________________________________________________________________________________________________ ________________________________________________________________________________________________ Section 8.2 – Structure of DNA KEY CONCEPT - DNA structure is the same in all organisms MAIN IDEA: DNA is composed of four types of nucleotides. VOCABULARY Nucleotide Double helix Base Pairing Rules 1. In the space at right, draw a nucleotide and label its three parts using words and arrows. 2. How many types of nucleotides are present in DNA? _______________________________________________________________________________________________ 3. Which parts are the same in all nucleotides? _______________________________________________________________________________________________ 4. Which part is different in all nucleotides? _______________________________________________________________________________________________ MAIN IDEA: Nucleotides always pair in the same way 5. What nucleotide pairs with T? _______________________________________________________________________________________________ 6. What nucleotide pairs with C? _______________________________________________________________________________________________ 7. In the space below, draw a DNA double helix. Label the sugar-phosphate backbone, the nitrogen-containing bases, and the hydrogen bonds Section 8.2 – Structure of DNA Parts of the DNA molecule Overall shape 14. Nitrogencontaining bases Pyrimidines 17. Purines Backbone 15. 18. 16. 19. 20. 21. Base pairing rules: A pairs with T G pairs with C Types of bonding 22 23. Chargaff’s rules: 24. Draw the complementary DNA strand Section 8.3 – DNA Replication KEY CONCEPT - DNA structure is the same in all organisms MAIN IDEA: Proteins carry out the process of replication VOCABULARY Replication DNA Polymerase DNA replication copies the genetic information of a cell 1. What is DNA replication? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 2. Where does DNA replication take place in a eukaryotic cell? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 3. When is DNA replicated during the cell cycle? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 4. If one strand of DNA had the sequence TAGGTAC, what would be the sequence of the complementary DNA strand? . __________________________________________________________________________________________________ ________________________________________________________________________________________________ 5. What roles do proteins play in DNA replication? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 6. What must be broken for the DNA strand to separate? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 7. Why DNA replication is called semiconservative? __________________________________________________________________________________________________ ________________________________________________________________________________________________ Use words and diagrams to summarize the steps of replication, in order, in the boxes below. 8. 9. 10. MAIN IDEA: Replication is fast and accurate 11. Human chromosomes have hundreds of origins of replications, where the DNA is unzipped so replication can begin. 12. DNA polymerase has a proofreading function that enables it to detect errors and correct them. 13. Why is DNA replication described as semiconservative? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 14. What are two major functions that DNA polymerase performs? __________________________________________________________________________________________________ ________________________________________________________________________________________________ 8.3- DNA Replication Match the following with the diagram below: 15. ______ Pairing of nitrogen-containing bases 16. ______ Sugar-phosphate backbone 17. ______ Newly synthesized strand of DNA 18. ______ Nitrogen-containing bases 19. ______ DNA polymerase
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