C146-E224B LC/MS/MS Rapid Toxicology Screening System Ver.2 LCMS-8040 Predetermined Analytical Conditions Semi-Quantitative Results without Authentic Standards This system provides MRM transitions, LC separation conditions, retention times, spectral libraries, and report ﬁles, enabling rapid implementation of a screening method for many important toxicological substances. By incorporating established calibration curves, this screening system provides laboratories the ability to perform semi-quantitative analysis without purchasing dozens of costly standards. Also included is an optimized sample preparation protocol for toxicology screening based on QuEChERS*. From sample preparation to reporting, a total solution for toxicology screening is provided. Screening for 161 Compounds Developed in conjunction with front-line researchers in the toxicology ﬁeld, the method analyzes several classes of substances. LCMS MRM optimization has been performed for common drugs of abuse, hypnotics, psychotropics, pharmaceuticals and other natural toxins. Shimadzu’s Ultra-Fast MS technology enables triggered product ion scans, which provide MS/MS data during MRM acquisition. * QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) was developed by Anastassiades et al in 2003 as a sample preparation method for residual pesticide analysis in agricultural products. HPLC Conditions (x100,000) Analytical Column : Kinetex XB-C18 (2.1 mmI.D. × 100 mmL., 2.6 μm) (Phenomenex) Guard Column : SecurityGuard Ultra C18 2.1mmID (Phenomenex) Mobile phase A : 10 mmol/L Ammonium formate + 0.1 % Formic acid in Water Mobile phase B : 10 mmol/L Ammonium formate + 0.1 % Formic acid in Methanol Flow rate : 0.3 mL/min Injection volume : 5 μL 4.0 Step Time (min) A (%) B (%) 0 0 95 5 1 7.5 5 95 2 10 5 95 3 10.01 95 5 4 15 95 5 Alprazolam Diazepam-d5 309.10>281.10(+) 309.10>205.10(+) 290.15>154.05(+) 290.15>198.20(+) 3.0 2.0 1.0 0.0 10.1 12.5 15.0 ,QWHQ [ P] LC/MS/MS Rapid Toxicology Screening System Ver.2 Compound Panel Registered substances include 161 compounds of illicit drugs, Hypnotics, psychotropics, pharmaceuticals and others that are often analyzed in the forensic medicine ﬁeld [IS] Diazepam-d5(for positive mode) Caffeine Estazolam Mepivacaine Promethazine [IS] Phenobarbital-d5 (for negative mode) Carbamazepine Ethenzamide Mequitazine Propericiazine 7-Aminoclonazepam Carpipramine Ethyl loﬂazepate Metformin Propofol (neg) 7-Aminoﬂunitrazepam Chlordiazepoxide Etizolam Methamphetamine Quazepam 7-Aminonimetazepam Chlorpheniramine Fenitrothion(MEP) Methomyl Quetiapine 7-Aminonitrazepam Chlorpromazine Fludiazepam Methylephedrine Risperidone 8-Hydroxyetizolam (M-III) Clobazam Flunitrazepam Methylphenidate Ropivacaine 9-Hydroxyrisperidone Clocapramine Flurazepam Mexazolam Salicylic acid (neg) Acetaminophen Clomipramine Fluvoxamine Mexiletine Sertraline Acetylpheneturide Clonazepam Gabapentin Mianserin Sildenaﬁl Aconitine Clotiazepam Glibenclamide Midazolam Spiperone Allylisopropylacetylurea Cloxazolam Gliclazide Milnacipran Sulpiride alpha-Hydroxyalprazolam Clozapine Glimepiride Mirtazapine Tadalaﬁl alpha-Hydroxybrotizolam Cocaine Haloperidol Morphine Tandospirone alpha-Hydroxymidazolam Codeine Haloxazolam Mosapramine Temazepam alpha-Hydroxytriazolam Colchicine Hydroxyzine Nemonapride THC Alprazolam Desipramine Ibuprofen Nicotine THC-COOH Amitriptyline Desmethylclotiazepam Imipramine Nimetazepam Thiamylal (neg) Amobarbital (neg) Desmethyldiazepam Ketamine Nitrazepam Timiperone Amoxapine Dextromethorphan Lamotrigine Nortriptyline Toﬁsopam Amphetamine Diazepam Levetiracetam Olanzapine Topiramate Aripiprazole Diclofenac Levomepromazine Oxazepam Trazodone Atropine Dihydrocodeine Lidocaine Paliperidone Triazolam Barbital (neg) Diltiazem Lorazepam Paroxetine Trihexyphenidyl Benzoyl ecgonine Diphenhydramine Lormetazepam Pemoline Vardenaﬁl Biperiden Diprophyline Loxoprofen (neg) Pentazocine Valproic Acid (neg) Blonanserin Diquat Malathion Pentobarbital (neg) Warfarin Bromazepam Donepezil Maprotiline Perospirone Zolpidem Bromocriptine Dosulepin MDA Perphenazine Zopiclone Bromovalerylurea Duloxetine MDMA Phenobarbital (neg) Zopiclone-N-oxide Bromperidol Ecgonine methyl ester Medazepam Pimozide Zotepine Brotizolam Ephedrine Mefenamic acid Pioglitazone Bupivacaine Escitalopram Memantine Primidone Remarks and Precautions 1. For Research Use Only. Not for Use in Diagnostic Procedures. 2. The information of calibration curve in this Method Package is created by LCMS-8040. 3. Any calculated concentrations obtained using this system are estimated values only. Because true values may differ signiﬁcantly due to sample status and system conditions, the estimates obtained are not valid for legal or medical decisions. To obtain accurate quantitative values, a validated method using appropriate standards and quality control procedures must be used. 4. Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database. 5. It is the user’s responsibility to adopt appropriate quality control tests using standard samples to conﬁrm qualitative and semi-quantitative information obtained with this method package. © Shimadzu Corporation, 2014