Pharmed Group – Introduction to our Biologics Division February 2015 Westmeath • Kildare • Dublin • Belfast • Oxford ASC Kit AutoPRP Kit Westmeath • Kildare • Dublin • Belfast • Oxford What? Regenerative Medicine : Healing Trinity Scaffold Collagen & HA Stem Cells Bone Marrow & Adipose Growth Factors Peripheral Blood (PRP) Westmeath • Kildare • Dublin • Belfast • Oxford How? Chondrocytes Osteoblasts MSCs Westmeath • Kildare • Dublin • Belfast • Oxford Why? Westmeath • Kildare • Dublin • Belfast • Oxford Theatre Protocol + Patient will be consented for collection of Peripheral Blood & Bone Marrow Sample + Normal Arthroscopic / Mini-Arthrotomy Procedure Time : 50 mins–75 mins (No further added time) + Autologus extraction of cells are processed by a centrifugation process and this would be implanted via a HA (Hyaluronic Acid) based scaffold or via Fibrin Glue which are already used across surgical operations. Westmeath • Kildare • Dublin • Belfast • Oxford Step 1 Extract Bone Marrow or Blood Sample using 5ml Luer Lock Syringe (approx 25‐30ml) Step 2 Transfer sample into the ‘Separation Kit’ Step 3 Unscrew the bottom half to balance level Step 4 1st Centrifuge Spin 2nd Centrifuge Spin Step 5 Extracting the Plasma & Buffy Layer Cells are ready to be implanted! Westmeath • Kildare • Dublin • Belfast • Oxford Development Projects Westmeath • Kildare • Dublin • Belfast • Oxford Our last patient… • • Patient Name: Mr X Harris DOB: 23 / 05 / 1968 Hospital ID: 246 5567 3773 Reference File : G677HU78 After 2 years MRI show a complete filling of the defect with regenerated tissue. Newly formed matrix analysis with T2 mapping indicates that the tissue has characteristics Westmeath • Kildare • Dublin • Belfast • Oxford Thank You Westmeath • Kildare • Dublin • Belfast • Oxford