A Bio Refining Centre of Excellence for Wales ADMIT BioSuccInnovate Adaptation and Mitigation through Bio-Succinate Innovation 12 partners; 4 Industrial & 7 academic, UK led with FR, NL, CHF & HU Dr David Bryant [email protected] What is Climate KIC? Bioeconomy Platform Overview Illustration by David Parkins KIC Value Proposition “Investment in public/private partnership projects to reduce carbon emissions & promote economic growth” The Ecosystem LCA & carbon stock management Xylitol C5 sugars C6 sugars Biomass lignin Biosuccinium New biotech process & product launched Improved climate change mitigation, greater market penetration & bioeconomic growth New biotech Polyurethane process & product precursors launched BioSuccInnovate Goals To demonstrate the production of biosuccinic acid from lignocellulosic sugars To determine the reduction of GHG emissions compared to Reverdias current process. To determine the techno-economic feasibility. Demonstrate the applicability of the CIMV biorefining process and provide commercial uplift. The biorefinery concept Feedstocks, logistics and pre-treatment processes for sustainable lignocellulosic biorefineries: A comprehensive review. Kurian et al., Renewable and sustainable energy reviews 25 (2013) 2015-219 Reverdia’s Cassano Facility for Biosuccinium Production Bio-Succinate market size Bio-succinate Market Growth & Potential What are the market sectors for Bio-SuccinniumTM? What are the market sectors for Bio-SuccinniumTM? Feedstock for BioSuccInnovate Can Bio-Succinate Mitigate Climate Change? New processing techniques Enterprise led PhD Innovation Team Life cycle and techno-economic assessment (with Uni of Geneva) Anna Suchy Lignin valorisation Greenhouse (with IBERS) funding CoPhenol C5 sugar David Walker valorisation, IB Greenhouse scale-up xylitol funding H2Oasis High Performance Computational protein modelling (with IBERS) Pretreatment and fermentation of xylose to xylitol David Walker Steam explosion rig by Cambi 30 L reaction vessel Flash tank Collection point High pressure boiler (to 35 bar) Pre-treated biomass from the flash tank SE is common scalable pretreatment for lignocellulosic biomass. Above material will liquefy in under 1 hour with standard cellulosic enzymes. Saccharification / Fermentation • Replace platform chemicals and biofuels traditionally produced from petrochemicals • Novel products Oral hygiene and obesity Roadmap for Xylitol Innovation • • Xylitol – a natural sweetener encompassing a $537 M dollar market Adding value to C5 sugar stream for enhanced commercial uptake of 2G lignocellulosic sugar platform Xylose reductase Substrate binding combinatorial design 5 aa full site saturation = 5^(20) combinations Need “smart” sensing genetic circuits and logic gates to select ara-ve. Need 2 of these strategies in place. Will use HPC modelling and redesign sensing components or “parts”. Identify xyl+ve. Enzyme Innovation Elenni Ioannou The market need : CIMV hemicellulose detoxification Improved biomass processing enzymes Enzymes EnZymovate High Performance Computing Functionally improved Chimeras US Market = $1.7 Bn by 2018 Economic or Environmental sustainability? Acknowledgements and thanks