Operational Cruise Plan - Laboratory for Microbial Oceanography

Center for Microbial Oceanography: Research and Education (C-MORE) and the University of Hawaii
Cruise Plan
Hawaii Ocean Experiment - Legacy I (HOE-Legacy I)
Dates: April 6-10th, 2015
Ship: R/V Kilo Moana, University of Hawaii
Master of the Vessel: Gray Drewry
Cruise ID: KM15-03
Chief Scientist: Tara Clemente
Co-Chief Scientist: Oscar Sosa
OTG Marine Technicians: Steve Tottori and Trevor Young
Kilo Moana phone number: 842-9817, cell 864-0065, satellite 001-870-336-956510
Marine Center phone number: 842-9813
Tara Clemente: 808-389-0544
Loading: April 2nd, 2015 @ 0900
Departure: April 6th, 2015 @ 0900 (Science personnel on board by 0800)
Arrival: April 10th, 2015 @ 0800
1.0 SCIENTIFIC OBJECTIVES
The objective of the cruise is to maintain a collection of hydrographic and biogeochemical data at the
Hawaii Ocean Time-series (HOT) station. Station ALOHA, is defined as a circle with a 6 nautical mile
radius centered at 22° 45'N, 158°W. Station ALOHA and will be occupied for a majority of the cruise.
1.1 SCIENTIFIC OPERATIONS
Station
ALOHA (sta. 2)
Activities
PIT and McLane Sediment traps, Primary Production array, CTD
operations, Hyperpro, misc. experiments.
Underway/continuous, ADCP, thermosalinograph, pCO2 system,
fluorometry, and meteorology.
1. Deployment of PIT Sediment trap array.
2. Deployment of bottom-moored sediment traps (2 x 4000m) in the vicinity of 22°51'N,
157°54W.
3. Deployment of Primary Production array.
4. CTD Operations
2.0 SCIENCE PERSONNEL
Participant
Frank Aylward (M)
Bob Bidigare (M)
Tara Clemente (F)
Maria del Carmin Muñoz Marin (F)
Ken Doggett (M)
Sara Ferron-Smith (F)
Eric Grabowski (M)
Christien Laber (M)
Morgan Linney (F)
Jozef Nissimov (M)
Sarah Searson (F)
Eric Shimabukuro (M)
Oscar Sosa (M)
William Truong (M)
Ger van den Engh (M)
Alice Vislova (F)
Blake Watkins (M)
Steve Tottori (M)
Trevor Young (M)
Title
Post-Doc
Scientist
Chief Scientist
Post-Doc
Research Associate
Post-Doc
Research Associate
Student
Research Associate
Post-Doc
Research Associate
Research Associate
Co-Chief Scientist
Intern
Scientist
Student
Marine Engineer
Marine Technician
Marine Technician
Affiliation
UH/CMORE
UH/CMORE
UH/SCOPE/CMORE
UCSC/CMORE/SCOPE
UH/CMORE
UH/CMORE
UH/CMORE
Rutgers University
UH/CMORE
Rutgers University
UH/CMORE
UH/SCOPE
UH/CMORE
UH/CMORE
MARCY
UH/CMORE
UH/CMORE
OTG
OTG
(19 science personnel: 13 male and 6 female)
3.0.
SUMMARY SCHEDULE
2 April
6 April
6 -9 April
10 April
Ship loading at 0900 hrs
Depart from Snug harbor at 0900 hrs. Science personnel on-board by 0800.
Safety briefing, orientation meeting 1000.
Station ALOHA operations.
Arrive back to Snug Harbor.
4.0 OPERATIONAL PLAN
4.1.
Station ALOHA (22°45'N, 158°W with 6 nm radius)
4.1.1. PIT Sediment trap array deployment RF Ch. 68, 157.425 MHz
Upon arrival to Station ALOHA, a floating sediment traps array will be deployed at a location
within Station ALOHA, to be determined en route to ALOHA by local current conditions. The
sediment trap array will be deployed from the stern, using the A-frame and large Sea-Mac
winch. Power requirement for the winch is 440 VAC, three phase at 10 amps. After deployment
we request that the bridge verify that the radio transmitters are functioning and directionally
correct. The PIT sediment trap array will be equipped with 2 ARGOS satellite transmitters
(platform #’s 3028, 60482), 2 strobe lights, and radio transmitters (RF Ch. 68, 157.425 MHz) and
will consist of 3 crosses 2 @150m and 1 @ another depth TBD. Daily positions of the array shall
be transmitted by email directly to the ship ([email protected], password: argosfix),
therefore the ship will not need to keep within sight of the array until the time of the recovery.
Assistance from the bridge is requested in plotting the drift track of the array. We request the
use of the ship's radio direction finder for locating the array before recovery.
NOTE: depending on the speed and distance this array travels, the time for recovery may shift to
maximize time for operations at Station ALOHA.
4.1.2.
Deployment of two McLane bottom-moored sediment traps in the vicinity of 22°51'N, 157°54W.
Two conical McLane sediment traps @ 4000m, glass floats and a mooring weight will be
deployed from the stern using the A-frame and large Sea –Mac winch (see attached diagram 1.)
Power requirement for the winch is 440 VAC, three phase at 10 amps. After deployment we
would like to triangulate the position of the deep-moored sediment traps.
4.1.3. On the first day at Station ALOHA (4/7), CTD casts will recommence.
4.1.4. Water column measurements
Vertical profiles of temperature, conductivity and dissolved oxygen will be made with the OTG
instrument package consisting of a Sea-Bird CTD attached to a 24-place rosette with 12 liter
Bullister sampling bottles. We will need the ship’s CTD winch and A-frame for these operations.
Water samples for biogeochemical measurements will be collected on each cast. There will be
an additional instrument to be included with the OTG CTD package –two Transmissometers with
a pump. Power requirement is 35-50 mA at 7-15V.
4.1.5. Primary Production Array deployment
A free drifting incubation array will be deployed once during this cruise, for 12- hour duration,
starting on the second day (4/7) of the cruise at Station ALOHA. We request the use of the Aframe for this operation and will also use the Sea-Mac winch. The array is equipped with two
ARGOS satellite transmitters (platform #’s 84857,60484) a strobe light and a radio transmitter
(RF Ch. 74, 157.725 MHz). Positions of the array shall be transmitted by email directly to the ship
([email protected], password: argosfix).
4.1.6. Hyperpro
The Hyperpro is a profiling unit with one up-looking and one down-looking hyperspectral
radiometer, a WET Labs ECO-BB2F triplet (measuring Chlorophyll-a fluorescence and
backscattering in the blue and red wavelengths), temperature and conductivity sensors. This
instrument also incorporates a ship mounted surface radiometer. The Hyperpro will be deployed
daily during the cruise. The Hyperpro is deployed from the stern through a small block hung
from the A-frame. The instrument is hand-lowered and retrieved. Each deployment will consist
of three profiles before the instrument is retrieved and take approximately 45 min to complete.
4.2
Acoustic Doppler Current Profiler
The ship’s acoustic Doppler current profiler (ADCP) will be in operation during the duration of
the cruise. The OTG electronics technicians will be in charge of the ADCP system.
Thermosalinograph, pCO2 system, Fluorometer, and meteorological system
4.3
The ship’s thermosalinograph, pCO2 system and fluorometer sampling the uncontaminated
seawater supply system will be in operation during the duration of the cruise while the ship is
outside of Snug Harbor. The ship’s meteorological system shall be in operation throughout the
cruise. Access to real-time underway data through the ship’s network will be required. The OTG
technicians will be in charge of the thermosalinograph, pCO2 system, Fluorometer, and
meteorological suite operations.
Whenever pumping of the ship’s tanks is needed, it must be conducted outside the circle that defines
station ALOHA (Sect. 1.0). To avoid disruptions in the schedule, this operation should be coordinated
with the chief scientist and co-chief scientist.
5.0
EQUIPMENT
5.1
The science party shall be bringing the following:
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
5.2
One 20 ft. laboratory van with assorted equipment for radioisotope and general use (Van #23)
One 20 ft laboratory van for radioisotope and flow cytometry use (Karl, FCM van)
All required chemicals and isotopes
Large vacuum waste containers
Liquid nitrogen dewars
Drifting PIT sediment trap array with strobe lights, satellite and radio transmitters, floats,
weights
Two McLane Sediment Traps with line, strobe lights, satellite and radio transmitters, floats,
weights
Primary Production array with strobe lights, satellite and radio transmitters, floats, weights
Polypropylene line
48 PIT Sediment traps and 4 crosses
Oxygen titration system
Desktop and laptop personal computers
Assorted tools
All required sampling bottles
Stand up Incubator
Deck incubation system
Pertinent MSDS
We will need the use of the following ship's equipment:
1. OTG’s 24-place rosette, and 24 12-l water sampling bottles
2. CTD Instrument package (OTG).
3. Two Transmissometers with pump on CTD instrument package.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
A-frame
A-frame block assembly
Winch and conducting wire for CTD operations (0.681)
Electric power for winches (440 VAC, 3 phase, 60 Amp breaker) and vans (208 VAC single phase
at 60 amps for lab van.
Radio direction finder
Space on upper deck for two laboratory vans
Space on upper deck for incubators
Hand-held VHF transceivers
Precision depth recorder
Shackles, sheaves, hooks and lines
Shipboard Acoustic Doppler Current Profiler
Thermosalinograph, pCO2 system, and Fluorometer
Meteorological suite
Copy machine
Grappling hooks and line
Laptop with Nobeltec charting software and GPS feed
Running fresh water and seawater hoses
Electronic mail system
GPS system
Uncontaminated seawater supply
Distilled, deionized water
Small capstan (~ 10 m/min)
Underway/on-station data acquisition system for meteorological instruments, ADCP,
thermosalinograph, fluorometer, pCO2 and access to real-time data through the network.
Large Sea-Mac winch (Mod. 1025 EHS). 60 Amp Hubbel plug/connector (440 VAC, 3 phase, 60
Amp breaker)
RV Kilo Moana
HOE-Legacy I
Date
Cast
4/7
S2C1
25m
S2C1
S2C3
CTD casts
April 6-10th, 2015
Target
Samples
Bottles
Depth
____________________________________________________________________________________________________________
Station ALOHA
OS (24@ 25m)
24
500 m
KB (Virus; 2 @ TBD, TBD, TBD, 55, 25 m, 1% Par); JZ(qPCR; 1 @ 5, 25, 45, 75, 100 m)
17
500 m
SF (2@ 5, 45, 75,100m & 4 @ 25);
12
500 m
KB(Virus; 2 @ TBD, TBD, TBD, 55, 25, 1% Par m); JZ(Diel; 2 @ TBD m);
23
Start Diel
4/7
S2C4
DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C5
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
23
SF pb DL (Diel; 200ml @ 5,25,45 m); SF (2@ 25m); EG ( Particulates 10@ 25m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m; 1 @ DCM)
S2C6
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
21
SF pb DL (Diel; 200ml @ 5,25,45 m); OS( 10@ 500m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C7
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
21
SF pb DL (Diel; 200ml @ 5,25,45 m) ; EG (Particulates 10@ 75m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C8
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
11
4/8
S2C9
500 m
KB(Virus; 2 @ TBD, TBD, TBD, 55, 25, 1% Par m); JZ(Diel; 2 @ TBD m);
23
DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM) SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C10
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
24
SF(4 @ 25m); SF pb DL (Diel; 200ml @ 5,25,45 m); EG (Particulates 10@ 350m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C11
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
23
SF pb DL (Diel; 200ml @ 5,25,45 m); SF (2 @ 25m); OS (10@ 500m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C12
500 m
KB(Virus; 2 @ TBD, TBD, TBD, 55, 25, 1% Par m); JZ(Diel; 2 @ TBD m);
23
DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM); SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C13
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
21
SF pb DL (Diel; 200ml @ 5,25,45 m); EG (Particulates 10@ 500m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C14
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
21
SF pb DL (Diel; 200ml @ 5,25,45 m); OS(10@ 500m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C15
1000 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
20
SF pb DL (Diel; 200ml @ 5,25,45 m); ML (1 @ 200,300,400,500,600,700,800,900,1000)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C16
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
SF pb DL (Diel; 200ml @ 5,25,45 m)
11
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
4/9
S2C17
500 m
KB(Virus; 2 @ TBD, TBD, TBD, 55, 25, 1% Par m); JZ(Diel; 2 @ TBD m);
23
DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM); SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C18
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
11
SF(4 @ 25m); SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C19
500 m
JZ(Diel; 2 @ TBD m); DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM)
18
SF pb DL (Diel; 200ml @ 5,25,45 m); SF (2@ 25m); ES (ESP-3 5@ 25m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
S2C20
500 m
KB(Virus; 2 @ TBD, TBD, TBD, 55, 25, 1% Par m); JZ(Diel; 2 @ TBD m);
DL (Diel; 500ml @ 1@5, 25,45,75,100,125,150,175m,DCM); SF pb DL (Diel; 200ml @ 5,25,45 m)
BB & KD pb DL (Diel; 2L @ 1@5, 25,45,75,100,125,150,175m,DCM)
Abbreviation users:
BB= Bob Bidigare, KB=Kay Bidle’s Group, KD= Ken Doggett, SF=Sara Ferron-Smith, EG=Eric Grabowski, DL=
Debbie Lindell, ML= Morgan Linney, ES= Eric Shimabukuro, OS= Oscar Sosa, JZ=Jon Zehr’s Group, Pb = piggy
back, i.e. shared Niskin bottle.
23
TIME
Mon.
4/6
Tues.
4/7
CTD Cast S2C1
0000
Wed.
4/8
Thurs.
4/9
CTD cast S2C8
CTD cast S2C16
CTD cast S2C9
CTD cast S2C17
Deploy Gas Array
CTD cast S2C10
CTD cast S2C18
Deploy bottommoored sed trap
Deploy Seaglider
Fri.
4/10
0100
CTD Cast S2C2
0200
0300
CTD Cast S2C3
0400
0500
0600
0700
Scientists onboard
0800
Scientists onboard
0900
Depart Snug
Transit ALOHA
Safety Briefing
Arrive at Snug
Harbor
CTD cast S2C11
CTD cast S2C19
CTD Cast S2C4
CTD cast S2C12
CTD cast S2C20
HyperPro
HyperPro
Recover Sediment
Traps
CTD cast S2C5
CTD cast S2C13
1800
CTD cast S2C6
CTD cast S2C14
1900
Recover
Gas Array
1000
1100
1200
1300
1400
1500
1600
1700
2000
Arrive ALOHA
2100
Deploy Sediment
Trap
2200
2300
CTD cast S2C7
CTD cast S2C15
Depart Station
ALOHA
18 (4/7/2015)
4000m depth