Plant Metabolomics and
Stable Isotopic Labeling
Workshop 2015
Mass Spectrometry Informed by Stable Isotope Methods th
5pm on Monday, July 20 to 24th 1 - 2pm on Friday Preceding Plant Biology 2015 at the Minneapolis Convention Center, (Annual meeting of the ASPB) which begins July 26th For college seniors, graduate students, faculty and scientists new to the area of plant metabolomics and proteomics Lead Instructors: Jerry D. Cohen, Adrian D. Hegeman, Paul G. Boswell, Stephen A. Brockman, Calvin P. Peters, Erin M. Jewett, Nathan D. Tivendale, Yuan Xu, and Dana M. Freund During this free 3½ day workshop, experienced instructors will give introductory and intermediate tutorials on the general workings and application of LC-‐ and GC-‐
mass spectrometry in the areas of plant metabolomics. Participants will also be introduced to on-‐line tools and other resources. Through hands-‐on demonstrations of typical metabolomic workflows, instructors will discuss the state of the art in metabolomics including current research being done at the University of Minnesota. Course Topics
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Registration for Plant Metabolomics Workshop 2015
Please go to http://goo.gl/forms/zDg86wfxXT to register by June 4th (hurry, space is limited to 15 individuals!) There is no registration fee for this workshop. Lunch and morning and afternoon snacks will be provided. Be sure to bring your own laptop! A limited number of SCHOLARSHIPS are available for transportation, room and board for special need students and postdocs. Please complete the Workshop Scholarship section of the registration form to apply for financial support. •
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General chromatography Gas chromatography Beginning and Intermediate HPLC Beginning and Intermediate Mass Spectrometry Stable isotope methods: Isotope dilution and tracer techniques Whole plant labeling methods Deuteration of polyphenolics Molecular turnover Beginning and Intermediate Metabolomics The workshop will be held in Alderman Hall on the St. Paul campus (1970 Folwell Avenue) WORKSHOP INFORMATION:
Meet the Instructors: Dr. Jerry D. Cohen Jerry Cohen earned his Ph.D. in Plant Biochemistry in 1979 from Michigan State University and did his postdoctoral work in biological mass spectrometry. His research career began with a focus on mass spectral techniques for quantification of plant hormones, metabolic pathways, as well as measurement of rates of metabolic turnover. He moved to his current position, professor and holder of the Gordon and Margaret Bailey Chair in the Department of Horticultural Science at the University of Minnesota, in 2000 and since that move has expanded into development of techniques for proteomic and metabolomic analyses. Dr. Adrian D. Hegeman Adrian Hegeman earned his doctorate in Mechanistic Enzymology at the University of Wisconsin–Madison in 2001. His current research in the Department of Horticultural Science at the University of Minnesota employs mass spectrometry for the high-‐throughput analysis of metabolites and proteins in plants. Ongoing projects involve using metabolic stable isotopic labeling of plants to gain novel insights into plant metabolism Dr. Paul G. Boswell Having earned his Ph.D. in the UMN Chemistry Department studying solution-‐phase chemical interactions, he is now a Research Assistant Professor in the University of Minnesota Horticultural Science Department. He is currently developing a new approach to harness HPLC retention for improved compound identification. Dr. Dana M. Freund Dana Freund earned her Ph.D. in Biochemistry at Colorado State University in 2013, where she conducted mass spectrometry-‐based proteomics research. Currently, she is a NSF postdoctoral fellow at the University of Minnesota in the Horticultural Science Department. Her research utilizes proteomics and metabolomics techniques measuring protein and metabolite turnover to investigate the regulation of plant metabolism by reversible post-‐translation modifications of proteins. Dr. Nathan D. Tivendale After earning his PhD in plant science and organic chemistry from the University of Tasmania in 2012, Nathan moved to the Cohen and Hegeman labs in 2013. Nathan’s research focuses on dynamic metabolic flux analysis in whole plants. The goal of Nathan’s research is to develop methods by which we can determine which fluxes are upregulated and downregulated under various conditions, which gives insight into the phenotypic response of the plant. Nathan is investigating the metabolic flux of a number of pathways, including amino acid biosynthesis and the TCA cycle, in the aquatic flowering plant Spirodela polyrhiza. Stephen A. Brockman Stephen is currently a Ph.D. student in the Hegeman lab at the University of Minnesota. His research is focused on the development and implementation of chromatography coupled mass spectrometry methodologies for the analysis of plants and fungi. Calvin P. Peters Calvin is a junior scientist who is developing in vivo isotopic labeling techniques for improving metabolic flux analysis. Currently, Calvin is engineering an automated multi-‐partition growth chamber to enable isotopic switching over time between a 12C and 13C label in parallel growth subsets. Yuan Xu Yuan is a Ph.D. student in Cohen lab in Plant Biological Sciences Graduate Program at University of Minnesota. Her research focuses on untargeted metabolomics study on abiotic stress responses of Arabidopsis thaliana. The goal of her research is to study the metabolic differences between control and stressed plants. Erin Jewett Erin is a Ph.D. student in the Hegeman lab in the Plant Biological Sciences Graduate program at the University of Minnesota. Her research focuses on metabolic flux analysis of central metabolism in three Brassica rapa morphotypes. During the workshop, we will be doing hands-‐on demonstrations. If you have samples that are applicable to our workshop topics and would like to run them while on campus, please contact us in advance. LODGING:
University of Minnesota Dorm Lodging: Please indicate interest on your registration form and we will contact you with the details. Payment will be required in advance. University Commons Hotel – Minneapolis: This hotel is conveniently located on the free University bus line called the Campus Connector. A bus comes by every 15 minutes and travels to the St. Paul campus where the workshop will be held. Reservations: 800-‐822-‐6757 http://www.commonshotel.com/ Radisson Hotel Roseville: This hotel is located less than 3 miles from the St. Paul campus and has a free shuttle service that can be scheduled. Reservations: 800-‐333-‐3333 http://www.radisson.com/roseville-‐hotel-‐mn-‐55113/mnroserd SPECIAL EVENTS AND ENTERTAINMENT:
Monday Evening, 5pm: Sign-‐in for the workshop and join us for wine tasting and dinner. Prepare to test your senses as you smell and taste metabolites! Wednesday Evening, 6pm: Drinks and dinner on the patio at Surly Brewing Co. in Minneapolis. Other Entertainment Opportunities in the Twin Cities: The University Commons Hotel is 3 minutes from downtown Minneapolis and provides easy access to several attractions, including the TCF Stadium, Target Field, and Mall of America. Once you arrive, there will be opportunities to join others for evening outings. This workshop is supported, in part, by NSF Plant Genome Research grants IOS-‐1238812 & IOS-‐1400818