TYPE THE PAPER TITLE HERE TYPE THE PAPER TITLE HERE TYPE THE PAPER TITLE HERE (TIMES NEW ROMAN, BOLD, 14 FONT, CENTER ALIGNED, CAPS) Author 11☼, Author 22… (Type the Author(s)’s Name Here) (Times New Roman, Bold, 12 font, center aligned) 1 Type the Affiliation with address here (Times New Roman, Italic, 11 font) 2 Type the Affiliation with address here (Times New Roman, Italic, 11 font) E-mail: [email protected] (Times New Roman, 11 font,) ABSTRACT (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, CAPS) (Times New Roman, italic, 11 font, justified) the abstract should be to the point and informative. It should not exceed 250 words in length. It should briefly explain the purpose of the work, techniques and methods used, major findings with important data and conclusions. Keywords: five to six keywords (Times New Roman, italic, 11 font, justified) ☼ Corresponding author (Times New Roman, 11 font,) INTRODUCTION (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, CAPS) (Times New Roman, normal, 12 font, justified, ) (Detailed instruction about this section is given. After reading these instructions, please delete this paragraph and begin typing your text here. They are known to exhibit potent antibacterial (Kumar et al., 1983; Desai and Naik, 2004; Desai and Desai, 2005), antifungal ( Aliasghar et al., 2007), anticonvulsant, anti inflammatory activities. In addition some Schiff base show pharmacologically useful activities like anticancer (Carson et al., 1995), antihypertensive and hypnotic (Sridhar et al., 2002) activities. Azetidinones which are part of antibiotics structure are known to exhibit interesting biological activities. A large number of 3-chloro monocyclic β-lactam possesses powerful antibacterial, antimicrobial ,anti-inflammatory, anticonvulsant and antitubercular activities. They also function as enzyme inhibitors and are effective on the central nervous system. (Freddy and Mishra, 2004) they are the carbonyl derivatives of azetidines containing carbonyl group at the position-2. These are also known as 2-azetidinones or more commonly β-lactam. 1 Azetidinones or β-lactam chemistry is of great importance because of the use of β-lactam derivatives as antibacterial agents. MATERIAL AND METHODS / EXPERIMENTAL DETAILS / METHODOLOGY (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, CAPS) (Times New Roman, normal, 12 font, justified) (Detailed instruction about this section is given. After reading these instructions, please delete this paragraph and begin typing your text here. All the chemicals used were of pure grade (Merck and B.D.H). The melting points of all compounds were determined by open capillary method and were uncorrected. RESULTS AND DISCUSSION (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, CAPS) (Times New Roman, normal, 12 font, justified) (Detailed instruction about this section is given. After reading these instructions, please delete this paragraph and begin typing your text here (National Sample Survey Office, 2010). In a 1000ml beaker, a mixture of (20 gm, 0.146mole) of anthranilic acid, 700ml of warm water (400 c) and glacial acetic acid (11ml, 0.19mole) was stirred mechanically and allowed to cool to room temperature. A freshly prepared solution of (15gm, 0.185mole) of potassium cyanate in 50ml of water was then added drop wise with stirring over a period of 15 to 20 minutes. The resulting pasty mixture was stirred for 20 minutes and then (200gm, 5mole) of Sodium hydroxide was added slowly in small portion. During this addition the reaction mixture was kept below 400 c by cooling in a cold water bath. A clear solution was obtained momentarily, but in a sort time a fine granular precipitate of the hydrated mono sodium salt of benzoylene urea precipitate. After the mixture has cooled over night in an ice box, the precipitated sodium salt was collected on a Buckner funnel. The colorless salt was dissolved in 1 lit of hot water and the solution was filtered. The compound was precipitated by adding dilute sulfuric acid.(1:1) with vigorous stirring until the liquor was acidic to litmus the material was collected, washed with water and dried in an oven at 100 0C (Thornsberry & Stalons,1975). Each figure should have a caption. The caption should be concise and typed separately, not In the figure area. Figures should be self-explanatory. Information presented in the figure should not be repeated in the table. All symbols and abbreviations used in the illustrations should be defined clearly. Figure legends should be given below the figures. A sample figure is given below (UNICEF, 2011). 2 1.0 M HCl 0.5 M KOH 800 Corrosion rate (mdd) 700 600 500 400 300 200 100 0 0 1 2 3 4 5 System Fig. 1. Effect of different doses of aqueous extract of Inhibitors Table-1: Physical, chemical and biological properties of experimental soil (0-20 cm) Code No. S. aureus P.aeruginosa E. coli S. pyogenus 2a 100 200 100 62.5 2b 100 500 500 100 2c 125 200 62.5 200 2d 125 200 200 250 Ampicillin 250 100 100 100 Chloramphen icol 50 50 50 50 *Moisture content on oven dry weight basis Subheading Subheading (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED) second point heading. After the mixture has cooled over night in an ice box, the precipitated sodium salt was collected on a Buckner funnel. The colorless salt was dissolved in 1 lit of hot water and the solution was filtered. The compound was precipitated by adding dilute sulfuric acid.(1:1) with vigorous stirring until the liquor was acidic to litmus the material was collected, washed with water and dried in an oven at 100 0C Sub-subheading (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, underlined)third point heading. After the mixture has cooled over night in an ice box, the precipitated sodium salt was collected on a Buckner funnel. The colorless salt was dissolved in 1 lit of hot water and the solution was filtered. The compound was precipitated by adding dilute sulfuric acid.(1:1) with 3 vigorous stirring until the liquor was acidic to litmus the material was collected, washed with water and dried in an oven at 100 0C (Radetsky et al.;1986). Sub-sub-subheading (TIMES NEW ROMAN, ITALICS, 12 FONT, LEFT ALIGNED) fourth point heading. After the mixture has cooled over night in an ice box, the precipitated sodium salt was collected on a Buckner funnel. The colorless salt was dissolved in 1 lit of hot water and the solution was filtered. The compound was precipitated by adding dilute sulfuric acid.(1:1) with vigorous stirring until the liquor was acidic to litmus the material was collected, washed with water and dried in an oven at 100 0C CONCLUSION (TIMES NEW ROMAN, BOLD, 12 FONT, LEFT ALIGNED, CAPS) (Times New Roman, normal, 12 font, justified) (Detailed instruction about this section is given. After reading these instructions, please delete this paragraph and begin typing your text here. This should briefly state the major findings of the study. ACKNOWLEDGEMENTS (Times New Roman, normal, 12 font, justified) (Detailed instruction about this section is given. After reading these instructions, please delete this paragraph and begin typing your text here. A brief acknowledgement section may be given after the conclusion. All sources of funding should be declared as an acknowledgement. REFERENCES Aliasghar, J., Eric, de C., Khalili, D. and Brunel, J. M. (2007). Synthesis, Antibacterial, Antifungal and Antiviral Activity Evaluation of Some New bis-Schiff Bases of Isatin and Their Derivatives, Molecules,12, 1720-1730. Carson, C. F., Hammer, K. A. & Riley, T. V. (1995). Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca alternifolia (tea tree oil), Microbios, 82(332), 181-185. Desai, K. G. & Desai, K. R. (2005). Synthesis of biologically active 2-azetidinones under microwave irradiation, Indian Journel of Chemistry, 44(B), 2093-2096. Desai, K. R. & Naik. B. D. (2004). Synthesis of some heterocyclic Schiff base and azetidinone compounds and their antibacterial activity, Asian J Chem, 16(3-4), 1749-1752. Freddy, H. H. & Mishra, S. J. (2004). Synthesis of some azetidi-2-ones and thiazolidin-4- ones as potential antimicrobial Agents, Indian J. Heterocycl. Chem., 13, 197-200. Kumar.A, Gurtu.S, Agrawal J.C, Sinha J.N, Bhargava K.P and Shanker K. J. Indian Chem. Soc., 1983, LX, 608-609. 4 Radetsky, M., Wheeler, R. C., Roe, M. H. & Todd J. K. (1986). Microtiter broth dilution method for yeast susceptibility testing with validation by clinical outcome, journal of clinical Microbiology, 24(4), 600-606. Sridhar, S. K., Pandeya, S. N. and Stables, J.P. (2002). Anticonvulsant activity of hydrazones, Schiff and Mannich bases of isatin derivatives Europian Journal of Pharmaceutical Science,6 (3), 129-132. Thornsberry, C. & Stalons, D. R. (1975). Broth-dilution method for determining the antibiotic susceptibility of anaerobic bacteria Antimicrob. Agents Chemotherapy. January, 7(1), 15-21. National Sample Survey Office., (2010). NSSO 64th Round 2007-08, National Statistical Organisation, Ministry of Statistics and Programme Implementation, New Delhi: Government of India. UNICEF (2011). The Situation of Children in India – A Profile, May, Unicef, New Delhi 5
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