ACUSYST-MAXIMIZER®
ACUSYST-MAXIMIZER®
Flexible, pilot-scale bioreactor:
80L or 160L fed-batch bioreactor equivalent
depending on disposable size in use
ACUSYST-miniMAX
™
Diagnostic and therapeutic
uses
Produce MAbs, recombinant proteins or virus
Culture suspension
or adherent
Process-development,
pilot-scale bioreactor
cell lines
80L fed-batch bioreactor equivalent
Single-use disposable:
- Supplied sterile and ready-to-use
Produce MAbs, recombinant proteins or virus
- No
cleaning
or expense
Culture suspension
or adherent
cellvalidation
lines
- Fast turnaround time
Single-use disposable:
Diagnostic and therapeutic uses
ACUSYST-
miniMAX ™
Cell Culture System
• Supplied sterile and ready-to-use
In validation
use worldwide
• No cleaning
or expensefor
• Fast turnaround time
many years producing regulated
biologics with very high reliability!
In use worldwide for many years producing
regulated biologics
with up
veryto
high
reliability!
Produces
~1gm/day
from routine cell lines
Produces up to ~1/2 gm/day from routine cell
Runs routinely last for months—maximizing
lines
your
production
uptime
Runs routinely
last for months—maximizing
your production uptime
Hollow Fiber Advantages
Protein Production and
Concentration in the
Bioreactor!
Mother Nature knows this already! Capillaries efficiently
perfuse dense tissues. Hollow fiber capillaries are
Hollow
Fiber Advantages
semipermeable
membranes (<60KDa MWCO). Nutrients
Mother
Nature
knows
already!
and
wastes
freelythis
exchange.
Cells, growth supplements
Capillaries efficiently perfuse dense tissues.
andfiber
products
are retained.
Products are simultaneously
Hollow
capillaries
are semipermeable membranes (10KDa MWCO).
Lactate
cross
CROSS
section
SECTION
produced and concentrated! Growth supplements are
Nutrients and wastes freely exchange.
needed in very small amounts to save $$$ and create purer
Glucose
Glutamine
O2
CO2
NH4
Cells, growth supplements and
products
are retained.
supernatant
than in other culture methods.
Products are simultaneously produced
and concentrated!
Growth supplements are needed in very
small amounts to save $$$ and create purer
supernatant than in other culture methods.
Creating the next generation of therapeutic and diagnostic products
Protein Production and
Concentration in the Bioreactor!
Biovest.com
ACUSYST-MAXIMIZER®
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Additional Features
Save Time & Money
MAXIMIZER automatically controls:
Automation = Less labor
•
•
•
•
• e.g., DMEM:F12 (no supplement)
• e.g., DMEM:F12
or/
~125L / with 5% FBS
~2L
month
monthmedia
Various commercial
serum-free
FACILITY
~ 5L /
week
Hollow Fiber Means
High Cell Density!
~4x1011 viable cells uniformly
distributed throughout the bioreactor.
Specifications &
Requirements
54.4 kg
18–26°C ambient
100% CO2, 14–16 psi
70 cm W x 66 cm D x 52 cm H
100–230 VAC, 50/60 Hz, 500 watts
~125 to
250L /
month
~2 to
4L /
month
Use very little growth supplement!
MAXIMIZER
160L Fed-Batch Reactor
Simple, Bench-Top,
100% CO2,
Standard electricity
Large skid, multiple
gases, CIP, SIP, etc.
Complexity!
Oxygenator
Cascading pH controller. MAXIMIZER
produces concentrated and optionally
clarified supernatant. Go directly from
bioreactor to purification!
Maximizer uses two feed media:
~ 0.15 L
week
SEED TRAIN
0.4L Inoculum
Simple static
culture methods
8L Inoculum
Complex, Expensive,
Time-consuming
MEDIA COSTS
EXAMPLE
$66/gm
MAb produced
$300/gm
MAb produced
DOWNSTREAM
PROCESS
26L of Supernatant
Supernatant is ready
for purification!
160L of Supernatant
Concentration,
Clarification… Expense & time
TURNAROUND
EXPENSE
Runs last months
Maximizes uptime
Disposable = Rapid
turnaround
10-Day Runs
Frequent cleaning and
startup costs
Lower productivity
Inside CO2 Incubator
Support
Oxygenator
pH
Incubator temperature
EC Cycling (tissue perfusion rate)
Continuous or batch harvesting
You focus on the science. We’ll focus on providing robust, easy-to-operate,
and low-maintenance bioreactors. Our bioreactors are successfully operated
by technicians with routine cell culture experience, not chemical engineers.
Our technical support assists customers with achieving their success.
Operates inside CO2 incubator
Biovest provides training, IQ/OQ, field service, and preventive maintenance
for
its bioeactors.
~
30L / week
Curt Gleiter, Client Service - Instruments
8500 Evergreen Blvd. | Minneapolis, Minnesota 55433 USA
(763) 786-0302 ext. 215 | [email protected]
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