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HF MicroBrx™
Hollow Fiber R&D Tool
New to hollow fiber technology?
Testing many clones or media?
Pursuing R&D or feasibility projects?
Static culture product concentration low?
HF MicroBrx is our patented, micro-sized hollow fiber
bioreactor for your R&D projects.
HF MicroBrx is ideal for rapidly testing of numerous
variables at low-cost, unlike larger cell culture systems!
HF MicroBrx is simple to use — like T-Flasks — and only
needs a CO2 incubator.
Experience the advantages of hollow fiber cell culture:
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Culture suspension or adherent cells
Cells naturally grow to tissue-density
Product concentrates as it’s secreted
Product is not diluted in the feed media
Collect usable product — even from low-secreting cell lines!
Biovest’s HF MicroBrx™: 0.2 mL culture volume, 0.003 m2
Biovest’s large-scale hollow fiber bioreactor: 155 mL culture volume, 2.1 m2 surface area
HF MicroBrx™
Hollow Fiber R&D Tool
EC Port
(non cell-side)
HF MicroBrx Design
One Bioreactor … Two Compartments
HF MicroBrx, like our large-scale hollow fiber bioreactor systems,
has two compartments that are separated from one another
IC Port
(cell-side)
by the semi-permeable (<60 KDa MWCO) membrane (wall)
of the hollow fiber.
ExtraCapillary (EC) Space
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IC
Volume outside of the hollow fibers
Large volume, ~4.5 mL
Functions as reservoir of small nutrients
Cell-free (non cell-side of hollow fiber wall)
Product-free
Cell culture medium in EC may or may not
contain growth supplements
EC
Glucose
IntraCapillary (IC) Space
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Porous Hollow
Fiber Wall
Combined volume inside all of the hollow fibers
Small volume, ~0.2 mL
Contains cell culture (cell-side of hollow fiber wall)
Contains concentrated product
Cell culture medium in IC contains growth supplements
(unless protein-free adapted)
Lactate
O2
Ammonia
CO2
Sil
ico
ne
Tu
bin
g
Glutamine
Rapid Experiments!
Day 0
Day 1
Fill & rinse with media, Place in CO2 incubator
Inoculate (low- or high-density condition)
• 0.2 mL cells (5x106/mL) to model growth phase
• 0.2 mL cells (5x107/mL) to model production phase
Day 2,3 Optionally
• change EC medium (high-density inoculum)
• assay metabolites, pH, DO
Day 4 Collect Data
• harvest & count cells
• assay metabolites, pH, DO
• assay and test product
CO2
Exchange
O2
Diffusion
Specifications & Requirements
Sold sterile in 3-pack with needed supplies
~1˝ (2.5 cm) diameter, ~9˝ (23 cm) long
CO2 cell culture incubator
Select Journal Citations
Low-Density Evaluation
• good growth is ~ 2 – 5x expansion in 3 days
High-Density Evaluation
• correlate production dynamics to cell number
Gramer, M.J., Britton, T.B. (2002) Antibody Production by
a Hybridoma Cell Line at High Density is Limited by Two
Independent Mechanisms. Biotech. Bioeng., 79, 277-283.
Gramer, M.J., Britton, T.B. (2000) Selection and isolation
of cells for optimal growth in hollow fiber bioreactors.
Hybridoma., 19, 407-412.
Curt Gleiter, Client Service - Instruments
www.biovest.com
8500 Evergreen Blvd. | Minneapolis, Minnesota 55433 USA
(763) 786-0302 ext. 215 | [email protected]