Methods of Determination for Mycotoxins July 20th – July 24th 2015 College Park, MD, USA Course Description: The International Food Safety Training Laboratory (IFSTL) is hosting a 5-day training workshop focused on methods of mycotoxins in foods on July 20-24th, 2015. What You Will Cover: - Familiarize with the regulations concerning mycotoxins in food and fit-forpurpose laboratory methods for sample preparations, identification and quantification - Review the relevant compliance programs from FDA and USDA - Learn and practice traditional and modern laboratory methods to prepare samples and standards - Run ELISA plate and lateral flow methods, LC-fluorescense and LC-MS/MS analyses. How You Will Benefit: - Understand the FDA and USDA mycotoxin compliance program and the impact on public health and the importation of food products - Prepare samples and standards for mycotoxin analysis by validated methods - Perform ELISA tests and lateral flow techniques - Understand the methods development and analysis using LC equipped with fluorescence and mass spectrometry detection SeekingAccreditation? - Learn the steps necessary to gain formal recognition of technical competence in your laboratory - Increase confidence in testing and calibration data by fulfilling accreditation criteria Instructors: This workshop is led by Dr. Mary Trucksess, world-renown expert in mycotoxins and includes the participation of subject matter experts. Guest lecturers in past workshops have included: - Dr Timothy Norden (USDA) - Dr. Chris Maragos (USDA) - Dr. Kathy D’Ovidio (US FDA) Who should attend? - Alex Krynitsky (US FDA) - Dr Kai Zhang (US FDA) This course is designed for laboratory - Dr Kristina Williams (US FDA) scientists and managers involved in testing - Dr Garnett Wood (US FDA) food for domestic markets and export to the - Dr Paul Turner (UMD) United States. Laboratory Training in Fit-For-Purpose Analytical Techniques for Food Safety International Food Safety Training Laboratory (IFSTL) Morning Lecture Afternoon Laboratory Session Monday Welcome and Technology Transfer FDA Regulation and Compliance USDA/GIPSA program Lab: Safety and sample preparation Lab: Sample and standard preparation Spectrometric measurements Tuesday Sampling for mycotoxins Principles of antibody and antigen reactions Introduction to ELISA Lab: ELISA plate method for aflatoxins Lateral flow method for deoxynivalenol Wednesday Regulatory limits of mycotoxins Quality Assurance and Quality Control Lab: Immunoaffinity column clean up Lab: Fluorescence detection Lab: TLC Thursday Principles of mass spectrometry Quantitative analysis Identity and confirmation Lab: UPLC/MS/MS method Friday Data analysis Technology transfer discussion Results and discussion Attendees are required to participate in the hands-on laboratory work including sample preparation and sample analysis. All safety equipment will be provided Course Information Tuition 𝑅𝑒𝑔𝑖𝑠𝑡𝑟𝑎𝑡𝑖𝑜𝑛 𝐷𝑒𝑎𝑑𝑙𝑖𝑛𝑒 𝐿𝑎𝑛𝑔𝑢𝑎𝑔𝑒 $2,500 USD 𝐽𝑢𝑛𝑒 20, 2015 𝐸𝑛𝑔𝑙𝑖𝑠ℎ (Includes all course material and lunches) Travel Participants responsible for travel arrangements and costs Registration Procedure 1. Fill out a registration form at: http://ifstl.jifsan.umd.edu/upcomingClasses/ 2. You will receive a formal letter of invitation to support your visa application. Information about airports, hotels, and local transportaiton will also be provided. 3. Payment is due by the registration deadline and seatsare only reserved upon payment. JOINT INSTITUTE FOR FOOD SAFETY AND APPLIED NUTRITION (JIFSAN) University of Maryland 5201 Paint Branch Parkway 2134 Patapsco Building College Park, MD 20742 301.405.1342 ifstl.org