Wakamine Potent whitening active Lighten your skin [De]pigmentation Lightening products are increasingly popular especially in Asia where they are part of the daily cosmetic habits. Some efficient ingredients may no longer be used in cosmetics: hydroquinone is now restricted to dermatology and kojic acid safety is more than questionable. New ingredients are therefore requested by the market. The pigmentation of the skin results from the presence of melanin in the epidermal keratinocytes. Melanin is synthesized in intracellular organoids (melanosomes) by specialized cells (melanocytes). Melanocytes transfer them to the keratinocytes. There are two types of melanin pigments: the eumelanin, which is black-brown and the pheomelanin which is yellow-orange. Tyrosinase is the key enzyme of these pigments’ synthesis. It leads to dopaquinone production. The dopaquinone reacts with cysteine entering the way of synthesis of the pheomelanin. If the quantity of cystein is weak in the cell, the dopaquinone oxidizes spontaneously in dopachrome and follows the way of the eumelanin synthesis. Therefore, tyrosinase is the key enzyme for pigmentation. Wakamine is a new active ingredient of marine origin, which inhibits tyrosinase and melanin production. Wakamine, an undaria pinnatifida extract Wakamine is extracted from a brown macro algae, Undaria Pinnatifida, stemming from the Laminaria family. This macro algae grows in fairly protected water such as bays. Undaria Pinnatifida originates from Japan where it is traditionally cultivated and used in Asian cooking, notably in Japan as Wakame. Accidentally introduced in France during the seventies, this macro algae is nowadays cultivated in Brittany and authorized in the food industry. Whitening effect Wakamine strongly inhibits melanin production (Ex vivo test) Epidermis are obtained from frozen skin coming from surgery of female patients. They are separated by incubation with NaBr 2N for 100 minutes at 37°C. They are fixed with a buffered formaldehyde solution, rinsed and treated with the mixture composed of the active to be tested and 1mg/ml L-DOPA solution during 4 hours at 37°C. After incubation, the epidermis are rinsed and viewed on a microscope. The pictures are quantified for melanin content. Wakamine inhibits L-Tyrosinase : -39% Control: melanocyte are visible Wakamine (100µg/ml) : melanocytes are slightly visible Wakamine induces a strong reduction of color skin (Ex vivo test) A solution of Wakamine 3% is applied on the skin explants with Whatmann paper patches. Explants are daily treated during 6 days. The explants are formol-fixed and paraffin-embedded. Melanin colouring on the biopsy sections was carried out according to the Masson’s technique. Color intensity is calculated by image analysis. Wakamine outperforms Arbutin with fast visible results INCI name Aspect Color Odor Preservative Water (and) Undaria Pinnatifida extract Clear liquid Light yellow Characteristic Phenoxyethanol Formulation lightening cream Phases Components % Xyliance Stearic Acid Macadamia ternifolia seed oil Dimethicone Aluminium Starch Octenylsuccinate Preservative 3 2 7 A B Water Glycerin C Sodium polyacrylate Ethylhexyl stearate trideceth-6 Approved by ECOCERT for ecological and organic cosmetics 3 2 qs qsp 100 2 0,5 D Wakamine 3 E Citric acid qs pH = 5,5 F Parfum qs Cosmetic uses Wakamine is recommended at 1-3 % in : • Skin lightening products • Age spots prevention 30 place de la Madeleine – 75008 PARIS (FRANCE) – Tel : +33 1 53 30 90 00 – Fax : +33 1 53 30 90 01 [email protected] – www.soliance.com WAK 0806/2-10/06
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