E-Notebook
CambridgeSoft
Introduction to
BioAssay
Protocols
CambridgeSoft Corp.
Cambridge, MA
USA
www.cambridgesoft.com
Kate Sydney
Manager, Marketing
Communications
Presentation Outline
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What is BioAssay?
How do I make BioAssay
work?
What is a protocol?
What are the data sources
for BioAssay?
Demo
How do I get access to
BioAssay?
Wh t sorts
What
t off licenses
li
to
t
BioAssay does
CambridgeSoft sell?
What is BioAssay?
Import and storage of
assay data
Automated handling of
assay workflows
kfl
Graphical
G
hi l outputs and
dh
heat
maps
Mathematical manipulation
and analysis of assay
information
How do I make BioAssay Work?
Example % Inhibition Experiment Workflow
Accept data
from plate
reader
Prompt for
plate ID,
notebook #,
scientist
name
Use predefined plate
format “80
compound
screen”
screen
Find
Fi
d positive
iti
and negative
control wells
and
calculate
average
Calculate %
inhibition
against raw
data in wells
Graph %
Inhibition
against
Compound
ID
What is a protocol?
A hierarchical
representation
t ti off
your experiment
workflow
Another way of looking at it
Plate
fx Pos/Neg Ctrl Avg
Y = Average (all wells with
Well ID = empty or pos
control)
Graph %Inhibition
Well
fx %Inhibition
Y = 100 * ((raw value – negative control
average)/(positive control average –
negative control average)
Data sources for BioAssay
Plate Setup Data
yp of p
g with. ((Ex: 96-well,
• Defines what type
plate yyou are working
384-well)
Well Setup Data
• Defines what substance is in each well of the plate. (Ex:
Empty, compound, negative control, positive control)
Protocol Setup Data
• Defines what actions and calculations you want to take place
upon import of data
Raw Data
• Can be virtually anything and can be in plate block format,
columnar data or a custom format that you can define
BIOASSAY DEMO
How do I get access to BioAssay?
What sorts of licenses to BioAssay does CambridgeSoft Sell?
Enterprise
p
software capabilities
p