E-Notebook CambridgeSoft Introduction to BioAssay Protocols CambridgeSoft Corp. Cambridge, MA USA www.cambridgesoft.com Kate Sydney Manager, Marketing Communications Presentation Outline What is BioAssay? How do I make BioAssay work? What is a protocol? What are the data sources for BioAssay? Demo How do I get access to BioAssay? Wh t sorts What t off licenses li to t BioAssay does CambridgeSoft sell? What is BioAssay? Import and storage of assay data Automated handling of assay workflows kfl Graphical G hi l outputs and dh heat maps Mathematical manipulation and analysis of assay information How do I make BioAssay Work? Example % Inhibition Experiment Workflow Accept data from plate reader Prompt for plate ID, notebook #, scientist name Use predefined plate format “80 compound screen” screen Find Fi d positive iti and negative control wells and calculate average Calculate % inhibition against raw data in wells Graph % Inhibition against Compound ID What is a protocol? A hierarchical representation t ti off your experiment workflow Another way of looking at it Plate fx Pos/Neg Ctrl Avg Y = Average (all wells with Well ID = empty or pos control) Graph %Inhibition Well fx %Inhibition Y = 100 * ((raw value – negative control average)/(positive control average – negative control average) Data sources for BioAssay Plate Setup Data yp of p g with. ((Ex: 96-well, • Defines what type plate yyou are working 384-well) Well Setup Data • Defines what substance is in each well of the plate. (Ex: Empty, compound, negative control, positive control) Protocol Setup Data • Defines what actions and calculations you want to take place upon import of data Raw Data • Can be virtually anything and can be in plate block format, columnar data or a custom format that you can define BIOASSAY DEMO How do I get access to BioAssay? What sorts of licenses to BioAssay does CambridgeSoft Sell? Enterprise p software capabilities p
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