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Do you know what’s in
your master mix?
Only Roche manufactures the enzyme you’ve always trusted.
Introducing EagleTaq Universal Master Mix from Roche, a ready-to-use, 2x PCR
master mix for use in real-time PCR. It’s the only master mix available produced by
the original manufacturer with the core polymerase technology used within the
TaqMan® Universal PCR Master Mix* – the original Roche-manufactured enzyme that
Life Technologies resold as AmpliTaq Gold® DNA polymerase in the research field.
Switch to EagleTaq Universal Master Mix and maintain
the consistency and excellence you’re used to with these
key benefits:
Use of well-established protocols that generate
consistent results
Research continuity and quality
Avoid extensive revalidation of key protocols
Replicate the performance of established assays
Replicate the performance of established assays
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Application
EagleTaq Universal Master Mix (ROX) contains all the
reagents (except primers, probes, and templates)
needed to perform quantitative, real-time PCR hydrolysis
probe reactions
Contains a hot-start DNA polymerase which allows reaction
setup at ambient temperature
Suitable for all real-time instruments on which a ROX
reference dye is needed for quantitative analysis
Includes dUTP so that it may be used with Uracil-DNA
Glycosylase (UNG) to prevent false positives arising from
carryover contamination
With this robust reagent, any PCR protocol optimization
is minimized
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Additional product information
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Delta Rn
Cycle Number
0
Vendor X
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TaqMan 7900HT
Eagle Universal MMx
Human Reference cDNA 1ng
EagleTaq Universal Master Mix is manufactured on the
same site (RMS, Branchburg) as Life Technologies’ original
TaqMan® Universal PCR Master Mix
Available in the following pack sizes: 1mL, 5mL and 10x 5mL
Maintain consistent performance through
same cycling conditions
ETUMM x 0.5 ng
ABIMM x 0.5 ng
ETUMM x 50 pg
ABIMM x 50 pg
ETUMM x 5 pg
ABIMM x 5 pg
ETUMM x 500 fg
ABIMM x 500 fg
Average CI
0,0
10,0
20,0
30,0
ACTB Gene Expression Assy Master Mix Comparison Average Ct Values
Want to see how EagleTaq Universal Master Mix can enable consistent
and reliable results in your environment?
Learn more and request a free sample at lifescience.roche.com/campaigns/eagletaq today.
For general laboratory use.
* EagleTaq is the research field equivalent of Roche’s AmpliTaq Gold ® diagnostic
enzyme and is also the same Roche-manufactured enzyme that was sold by
Life Technologies Corporation as AmpliTaq Gold ® in the research field.
EAGLETAQ is a trademark of Roche. AMPLITAQ GOLD and TAQMAN are trademarks of
Roche and exclusively licensed to Life Technologies Corporation in the research field
and exclusively used by Roche in diagnostics.
Published by
Roche Diagnostics GmbH
Sandhofer Straße 116
68305 Mannheim
Germany
© 2014 Roche Diagnostics.
All rights reserved.