NxtScript Reverse Transcriptase
Designed for High Thermostability
higher cDNA yield of full-length transcripts than wild type
M-MLV Reverse Transcriptase.
R everse transcribe difficult templates
The high thermostability of NxtScript allows reactions
up to 60°C to overcome RNA secondary structures
(e.g., in GC-rich templates).
Achieve high sensitivity
NxtScript lacks endonuclease activity and has lower RNase
activity than AMV reverse transcriptases.
Achieve high specificity
Take advantage of the wide temperature activity range
of NxtScript to reverse transcribe at the temperature that
is optimal for your RNA target.
Benefit from high quality standards and
multiple formats
Manufactured in an ISO13485 certified facility ensuring full
documentation and traceability. Customized manufacturing
and filling is available upon request.
High Thermostability
45  Undiluted
40  1:10
35 Crossing Point
NxtScript is a designed M-MLV reverse transcriptase with
high thermostability. The enzyme can be used to generate
first-strand cDNA from mRNA or total RNA for use in downstream applications such as two-step RT-PCR, RT-PCR for
detection of viral and mRNA targets (e.g. cancer biomarkers),
and generation of full-length cDNA libraries. Point mutations
increase the thermostability of the enzyme and support
 1:100
30  1:1000
25 20 15 10 5 0 45°C
50°C
55°C
60°C
Reverse Transcription Temperature [°C]
Figure 1: Reverse transcription at different temperatures and
subsequent qPCR show the high thermostability of NxtScript.
NxtScript Reverse Transcriptase is for further processing only.
NxtS
Mast cript
er
c o m i Mix
ng
soon
Ordering Information
Product
Catalog Number
Pack Size
NxtScript Reverse Transcriptase, 250 U/µl
07 051 166 103
custom fill
Origin
Mutant from Moloney Murine Leukemia Virus, expressed in E. coli
Unit of Measure
MU
Storage Buffer
Tris/HCl, 25 mmol/l; Glycerin 50% (v/v); NaCl, 100 mmol/l; DTT, 20
mmol/l; EDTA, 0.1 mmol/l; Triton X-100, 0.01% (v/v), pH 7.5
Appearance
Clear colorless solution
Concentration
≥ 250 U/µl
Endonucleases
Not detectable
RNases
Not detectable
Stability
12 months at -15 to -25°C
Related Products
Product
Catalog Number
Pack Size
AptaTaq DNA Polymerase, 5 U/µl
05 457 882 103
custom fill
AptaTaq DNA Polymerase, glycerol-free, 50 U/µl
05 187 605 103
custom fill
FastStart Taq DNA Polymerase, GMP Grade, 5 U/µl
04 659 163 103
5 KU
FastStart Taq DNA Polymerase, 5 U/µl
12 161 508 103
custom fill
FastStart Taq DNA Polymerase, 100 U/µl
04 433 785 103
custom fill
EagleTaq DNA Polymerase, 5 U/µl
05 206 944 190
05 206 952 190
1 kU (0.2 ml)
25 kU (5 ml)
HawkZ05 DNA Polymerase, 40 U/µl
05 230 322 190
05 230 349 190
5 kU (0.125 ml)
200 kU (5 ml)
HawkZ05 DNA Polymerase, 40 U/µl, low glycerol
06 668 577 190
06 668 569 190
5 kU (0.125 ml)
200 kU (5 ml)
Hot Start Polymerases
Hot Start Polymerases with RT Activity
NxtScript Reverse Transcriptase is for further processing only.
For more information, please visit
custombiotech.roche.com
Your Roche Custom Biotech Customer Service
Europe, Middle East, Africa, Latin America
Phone +49 621 759 8580
Fax +49 621 759 6385
[email protected]
United States
Phone +1 800 428 5433, ext. 14649 (toll-free)
Fax +1 317 521 4065
[email protected]
Japan Phone +81 3 5443 5285
Fax +81 3 5443 7934
[email protected]
Canada Phone +1 450 686 7050
Fax +1 450 686 7012
[email protected]
Asia Pacific Phone +65 6371 6638
Fax +65 6371 6601
[email protected]
Published by
Roche Diagnostics GmbH
Sandhofer Straße 116
68305 Mannheim
Germany
© 2014 Roche Diagnostics.
All rights reserved.
 1014